immunohistochemical staining for ki67  (Proteintech)

Journal: The Journal of Comparative Neurology

Article Title: The Molecular Architecture of Somatic Spines of the Lateral Septum

doi: 10.1002/cne.70195

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Article Snippet: Synaptopodin , IHC: 1:250 , Rabbit , Synaptic Systems , 163002.

Techniques:

Journal: The Journal of Comparative Neurology

Article Title: The Molecular Architecture of Somatic Spines of the Lateral Septum

doi: 10.1002/cne.70195

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Article Snippet: Synaptopodin , IHC: 1:200 , Guinea pig , Synaptic Systems , 163004.

Techniques:

Journal: bioRxiv

Article Title: Impaired astrocyte-to-neuron cholesterol trafficking drives synaptic dysfunction in Rett syndrome

doi: 10.64898/2026.07.29.741426

Figure Lengend Snippet: ( A ) Representative images of primary branches from WT and Mecp2 HET neurons, treated for 24 hours with cholesterol or with vehicle (0.1 μg/ml), and immunostained for Synapsin1/2 (Syn1/2; green), Shank2 (red) and Map2 (white). Scale bar=5 µm. ( B-D ) Violin plots indicate the median (dashed line) and 25 th and 75 th percentiles (dotted lines) of Synapsin1/2 (B), Shank2 (C) and colocalized puncta number (D). Values for puncta number are expressed as percentages with respect to untreated WT neurons. n=57/69. Statistical analysis was performed by 2-way Anova, followed by Sidak post-hoc test; **p<0.01, ***p<0.001. ( E ) Representative images of axon initial segment (AIS) stained with anti-AnkyrinG (AnkG; red). Scale bar=20 µm. ( F,G ) Violin plots indicate the median (dashed line) and 25 th and 75 th percentiles (dotted lines) of AIS length in WT and Mecp2 HET neurons (at DIV14) after cholesterol supplementation or treated with vehicle (F). The frequency distribution of values of AIS length is shown in (G). n=70/exp.groups. ( H ) Violin plots indicate the median (dashed line) and 25 th and 75 th percentiles (dotted lines) of the distance of AIS from the soma in WT and Mecp2 HET neurons (at DIV14) after cholesterol supplementation or treated with vehicle. n=70/exp.groups. *p<0.05, by 2-way Anova, followed by Sidak post-hoc test;

Article Snippet: An immunostaining with AnkyrinG (#386005, Synaptic System; 1:1000) was performed to detect axon initial segment (AIS) in cortical neurons.

Techniques: Staining

Journal: Nature Communications

Article Title: Low-intensity pulsed ultrasound-mediated nose-to-brain co-delivery of β-blockers and aPD-L1 enhances glioblastoma immunotherapy

doi: 10.1038/s41467-026-76103-4

Figure Lengend Snippet: a was created by Figdraw. a , b Treatment schedule of the GL261 mouse brain tumor model (by figdraw.com). The detailed treatment sequence and time points. c , d Brain images of each group of mice obtained by T2 magnetic resonance imaging (MRI) with different treatments, where tumors are circled in white dashed lines. e-g Immunohistochemical images of tumor sections were examined at 15 days (Ki67 and PCNA, n = 5). h Survival curves of GBM tumor-bearing mice in different treatment groups ( n = 10). Survival analysis was calculated using the log-rank test. i Body weight changes of GBM tumor-bearing mice in different treatment groups ( n = 10). j , k Evaluation of antitumor effects of different treatments. n = 5 samples per group for data in ( c-f ) and ( i-k ). Data are expressed as the mean ± SD. Statistical significances were determined using one-way ANOVA with Fisher’s LSD post‑hoc test. Comparisons were performed between different groups in ( c-f ) and ( i-k ). Not significant (NS) is P ≥ 0.05, and significant P values are shown. n = 10 samples per group for data in ( h ). Survival analysis was compared using the log-rank test. Source data are provided as a file.

Article Snippet: Immunohistochemical staining utilized PCNA (AC058, PCNA Mouse mAb, Abclonal) and Ki67 (A26755PM, Ki67 Rabbit PolymAb®, Abclonal) antibodies, with technical assistance from Nanjing Youmeng Biotechnology Co., Ltd. Tumor sections underwent immunofluorescence staining using a TUNEL assay kit (Beyotime Biotech, Shanghai, China).

Techniques: Sequencing, Magnetic Resonance Imaging, Immunohistochemical staining