mouse α human igg3 hinge (SouthernBiotech)

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SouthernBiotech mouse α human igg3 hinge

B cell−extrinsic elevation of circulating plasmablasts and increase in IgE isotype switching in the patient. (A) Flow plots of main B cell subsets (top row) and antigen-experienced B cell subsets (bottom row) in one healthy control (left panels) and the patient (right panels). Representative of two independent analyses. (Data shown in rows 1 and 2, column 1 are identical to data in , row 1, column 4 and 5.) (B) Gate frequencies for antigen-experienced B cell subsets of five healthy controls (HCs) and the patient. Representative of two independent analyses. (C) Fraction of IgE class-switched cells among antigen-experienced B cell subsets of three healthy controls and the patient. The individual points for healthy control and patient represent one each of three sampling methods (conventional Ficoll prep, separator tubes, and whole-blood RBC lysis) from one experiment. (D) Schematic overview of iGB culture setup (created with BioRender). (E) Cell counts for one representative of two similar experiments. (F) IgM, IgG1, <t>IgG3,</t> and IgE secretion in cultures, normalized to cell count. Full gating strategies are shown in . Statistics were calculated using the unpaired t test. * = P < 0.05. aMBC_PC, aMBC/plasma cell; DN, double-negative; MBC_only, conventional memory B cell; SWmem, switched memory; T1, T2, T3, transitional 1, 2, 3 subsets.

Mouse α Human Igg3 Hinge, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg3/Mouse+Anti-Human+IgG3+Hinge-UNLB/pmc12955778-336-35-39
Average 93 stars, based on 9 article reviews

mouse α human igg3 hinge - by Bioz Stars, 2026-09

93/100 stars

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1) Product Images from "Oncostatin M receptor deficiency as a novel candidate genetic cause of autosomal recessive hyper-IgE syndrome"

Article Title: Oncostatin M receptor deficiency as a novel candidate genetic cause of autosomal recessive hyper-IgE syndrome

Journal: Journal of Human Immunity

doi: 10.70962/jhi.20250119

Figure Legend Snippet: B cell−extrinsic elevation of circulating plasmablasts and increase in IgE isotype switching in the patient. (A) Flow plots of main B cell subsets (top row) and antigen-experienced B cell subsets (bottom row) in one healthy control (left panels) and the patient (right panels). Representative of two independent analyses. (Data shown in rows 1 and 2, column 1 are identical to data in , row 1, column 4 and 5.) (B) Gate frequencies for antigen-experienced B cell subsets of five healthy controls (HCs) and the patient. Representative of two independent analyses. (C) Fraction of IgE class-switched cells among antigen-experienced B cell subsets of three healthy controls and the patient. The individual points for healthy control and patient represent one each of three sampling methods (conventional Ficoll prep, separator tubes, and whole-blood RBC lysis) from one experiment. (D) Schematic overview of iGB culture setup (created with BioRender). (E) Cell counts for one representative of two similar experiments. (F) IgM, IgG1, IgG3, and IgE secretion in cultures, normalized to cell count. Full gating strategies are shown in . Statistics were calculated using the unpaired t test. * = P < 0.05. aMBC_PC, aMBC/plasma cell; DN, double-negative; MBC_only, conventional memory B cell; SWmem, switched memory; T1, T2, T3, transitional 1, 2, 3 subsets.

Techniques Used: Control, Sampling, Red Blood Cell Lysis, Cell Characterization, Clinical Proteomics

Incubation:

Article Title: Induction of Autoimmune Myocarditis in Diversity Outbred Mice
Article Snippet: Plates were washed with 1× PBS containing 0.05% Tween-20 (Polysorbate 20) (Fisher Scientific, Waltham, MA, USA; cat. no. BP337500 ) and blocked with 200 μL of assay buffer (1× PBS containing 2% bovine serum albumin and 5% normal goat serum) for 1.5 h at room temperature. .. Serum samples diluted 1:100 were then added in duplicates and incubated at 37 °C for 1 h. After washing, horseradish peroxide (HRP)-labeled goat anti-mouse total Ig, IgM, IgG1, IgG2a, IgG2b, and IgG3 (cat. no. 5300-05), as well as IgG2c (cat. no. 1079-05), diluted 1:500 in assay buffer, were added as secondary antibodies (Southern Biotech, Birmingham, AL, USA). .. The plates were incubated at room temperature for 2 h, followed by washing and the addition of 100 μL of 1× tetramethylbenzidine substrate solution (Rockland Immunochemicals, Pottstown, PA, USA; cat. no. TMBE-RED-1000).

Article Title: Vaccination-induced neutralizing antibodies in immunocompetent hosts correlate with protection against rickettsiae
Article Snippet: .. Isotype-specific detection was performed by 1 h of incubation with horseradish peroxidase (HRP)-conjugated monoclonal antibodies to mouse IgG (1030-05, SouthernBiotech), IgG1 (1071-05, SouthernBiotech), IgG2a (1080-05, SouthernBiotech), IgG2b (1091-05, SouthernBiotech), IgG3 (1101-05, SouthernBiotech), or IgA (1040-05, SouthernBiotech) diluted 1:5000 in 3% BSA. .. After incubation, plates were washed five times with wash buffer and incubated with 100 μL of TMB substrate solution (34028, Thermo Fisher Scientific).

Article Title: Induction of Autoimmune Myocarditis in Diversity Outbred Mice.
Article Snippet: Plates were washed with 1× PBS containing 0.05% Tween-20 (Polysorbate 20) (Fisher Scientific, Waltham, MA, USA; cat. no. BP337500) and blocked with 200 μL of assay buffer (1× PBS containing 2% bovine serum albumin and 5% normal goat serum) for 1.5 h at room temperature. .. Serum samples diluted 1:100 were then added in duplicates and incubated at 37 ◦C for 1 h. After washing, horseradish peroxide (HRP)-labeled goat anti-mouse total Ig, IgM, IgG1, IgG2a, IgG2b, and IgG3 (cat. no. 5300-05), as well as IgG2c (cat. no. 1079-05), diluted 1:500 in assay buffer, were added as secondary antibodies (Southern Biotech, Birmingham, AL, USA). .. The plates were incubated at room temperature for 2 h, followed by washing and the addition of 100 μL of 1× tetramethylbenzidine substrate solution (Rockland Immunochemicals, Pottstown, PA, USA; cat. no. TMBE-RED-1000).

Binding Assay:

Article Title: Deletion of Ptpn2 in B cells promotes autoimmunity via TLR and JAK/STAT signaling
Article Snippet: Between all steps, the plates were washed 4 times with PBS containing 0.05%Tween-20 (wt/vol.) (Sigma-Aldrich). .. Horseradish peroxidase–conjugated goat anti-mouse IgM, IgA, IgG, IgG1, IgG2b, IgG2c, or IgG3 (Southern Biotech, 5300-05) was added to the cells, followed by development with 3,3’,5,5’-tetramethylbenzidine (TMB) (Invitrogen, 002023), used to detect antibody binding. .. Reactions were stopped using 1M HCL (Sigma-Aldrich, H9892).

Article Title: Deletion of Ptpn2 in B cells promotes autoimmunity via TLR and JAK/STAT signaling.
Article Snippet: Between all steps, the plates were washed 4 times with PBS containing 0.05%Tween-20 (wt/vol.) (Sigma-Aldrich). .. Horseradish peroxidase–conjugated goat anti-mouse IgM, IgA, IgG, IgG1, IgG2b, IgG2c, or IgG3 (Southern Biotech, 5300-05) was added to the cells, followed 1 4 R E S E A R C H A R T I C L E JCI Insight 2025;10(24):e196144 https://doi.org/10.1172/jci.insight.196144 by development with 3,3’,5,5’-tetramethylbenzidine (TMB) (Invitrogen, 002023), used to detect antibody binding. .. Reactions were stopped using 1M HCL (Sigma-Aldrich, H9892).

Bioprocessing:

Article Title:
Article Snippet: 3 nature portfolio | rep orting sum m ary M arch 2021 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples For immunolocalization in human biopsy samples, the following antibodies were used: collagen IV (goat, 1:400, Southern Biotech 1340-01), human IgG (Cy2 or Cy3 donkey anti-human IgG, 1:200; Jackson ImmunoResearch Laboratories ), C2 (mouse, 1:10; Santa Cruz, sc373809), C4b (rabbit, 1:500; Abcam ab181241), CFB (rabbit, 1:20; Proteintech 10170-1-AP), C3b (mouse, 1:1000; Abcam ab11871), C1q (rabbit, 1:1500; Dako A0136), C1q (goat serum, 1:400; Complement Technology A200), MBL (rabbit, 1:800; Abcam ab190834). .. For the localization of human IgG subclasses, monoclonal antibodies against IgG1 (mouse, 1:100, SouthernBiotech, 9052-01, clone no. 4E3), IgG2 (mouse, 1:40000, SouthernBiotech, 9080-01, clone no. HP6014), IgG3 (mouse, 1:1000, SouthernBiotech, 9210-01, clone no. HP6050), and IgG4 (mouse, 1:4000, SouthernBiotech, 9200-09, clone no. HP6025) were used. .. For immunolocalization in mice, the following antibodies were used: nephrin (guinea pig, 1:200; Acris Antibodies BP5030), neph1 (guinea pig, 1:200; kindly provided by Florian Grahammer), laminin (rabbit, 1:1000; Sigma-Aldrich L9393), WGA-rhodamin (1:400; Vector-Laboratories), THSD7A (goat, 1:200; Santa Cruz Biotechnology sc163455 or rabbit, 1:200; Sigma-Aldrich HPA000923), C1q (goat serum, 1:400; Complement Technology A200), C3 (FITC goat anti-C3, 1:100; Cappel 55500), C4d (rabbit, 1:50; Hycultec HP8033), C5b-9 (rabbit, 1:200; Abcam ab55811), CFB (goat, 1:50; Complement Technology A235), CFH (goat, 1:100; Complement Technology A237), MBL (rabbit, 1:800; Abcam ab190834), murine IgG (Cy2 donkey anti-mouse IgG H+L, 1:200; Jackson ImmunoResearch Laboratories), murine IgG subclasses (IgG1, IgG2a, IgG2b, IgG3) (goat, 1:5000; Rockland 610-101-040, 610-101-041, 610-101-042, 610-101-043, respectively), synaptopodin (guinea pig, 1:200; Synaptic Systems 163004 or rabbit, 1:400; Santa Cruz 50459), DACH-1 (rabbit, 1:100; Sigma-Aldrich HPA012672), 8-oxoguanine (goat, 1:600; Abcam ab10802).

other:

Article Title: Low-dose tamoxifen ameliorates ovariectomy-induced metabolic and immune dysfunction.
Article Snippet: Following washing, HRP-conjugated goat anti-mouse IgG, IgG1, or IgG3 (SouthernBiotech, Cat. No. 1030–05, 1070–05, and 1100–05, respectively) was applied for 1 h at room temperature in the dark.

Article Title: Automated endpoint titer ELISAs for high-throughput immunogenicity evaluations using a BioMek i7 liquid handler
Article Snippet: , IgG3 , HRP-Goat anti-Mouse IgG3 , Southern Biotech 1100–05 , 1:4000.

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