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Proteintech apaf1
Apaf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igf2bp3/IGF2BP3+Antibody/pm41787385-145-38-41
Average 96 stars, based on 208 article reviews

apaf1 - by Bioz Stars, 2026-09

96/100 stars

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Incubation:

Article Title: CircRNA‐TMEM71 Inhibits Human Aortic Smooth Muscle Cell Ferroptosis and Prevents Aortic Dissection Formation Through IGF2BP3‐Mediated Stability of FSP1
Article Snippet: Afterwards, using skim milk (Solarbio, China), membranes were blocked for 2 hours at room temperature. .. Incubation at 4 °C was performed with antibodies against caspase- 3 (Proteintech, China), Bcl- 2 (BCL2 apoptosis regulator, Proteintech), BAX (BCL2 associated X, apoptosis regulator, Proteintech), SLC7A11 (solute carrier family 7 member 11, Proteintech), GPX4 (Proteintech), FSP1 (Proteintech), and IGF2BP3 (Proteintech) overnight. .. GAPDH (Proteintech) was used as an endogenous reference protein, The antibody information required for Western blotting analysis is listed in Table S3.

Article Title: The METTL3-IGF2BP3 axis drives osteosarcoma progression by enhancing ID1 mRNA stability
Article Snippet: Equal protein samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (IEVH85R, Millipore), which were blocked with 5% DAB for 1 h at room temperature. .. After that, membranes were incubated overnight at 4 °C with primary antibodies against ID1 (1 : 1,000, 18475-1-AP, Proteintech), IGF2BP3 (1 : 10,000, 81805-1-RR, Proteintech), METTL3 (1 : 1,000, #86132, Cell Signaling Technology), and GAPDH (1 : 10,000, 10494-1-AP, Proteintech). .. Following washing three times with Tris-buffered saline with Tween-20 (TBST), incubation was continued with HRP-conjugated goat anti-rabbit IgG secondary antibody (1 : 1,000, A0208, Beyotime) for 2 h at room temperature.

Article Title: LncRNA EGFR-AS1 inhibits ferroptosis to reduce radiosensitivity of cervical cancer through the m6A/IGF2BP3/APAF1 axis.
Article Snippet: .. Subsequently, the 198 sections were incubated with primary antibodies against ACSL4 199 (ab155282, Abcam), and GPX4 (ab125066, Abcam) IGF2BP3 (14642- 200 1-AP, Proteintech), and APAF1 (sc-65891, Santa Cruz) over night at 201 4 °C. ..

Article Title: Development and external validation of a mitophagy-related diagnostic model for biliary atresia based on cellular infiltration patterns
Article Snippet: .. Next, sections were incubated overnight at 4 °C with the following primary antibodies: anti-human ALDH2 (HUABIO, Hangzhou, China; M1509-1, 1:800), NEDD4L (Proteintech, Wuhan, China; 13690-1-AP, 1:800), and IGF2BP3 (Proteintech, Wuhan, China; 14642-1-AP, 1:800). .. Then, sections were incubated with horseradish peroxidase (HRP)-labeled secondary antibody (Abcam, Cambridge, UK; ab6721) for 30 min at room temperature followed by detection using DAB substrate and hematoxylin.

Article Title: The METTL3-IGF2BP3 axis drives osteosarcoma progression by enhancing ID1 mRNA stability.
Article Snippet: Equal protein samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (IEVH85R, Millipore), which were blocked with 5% DAB for 1 hour at room temperature. .. After that, membranes were incubated overnight at 4°C with primary antibodies against ID1 (1 : 1,000, 18475-1-AP, Proteintech), IGF2BP3 (1 : 10,000, 81805-1-RR, Proteintech), METTL3 (1 : 1,000, #86132, Cell Signaling Technology), and GAPDH (1 : 10,000, 10494- 1-AP, Proteintech). .. Following washing three times with Tris-buffered saline with Tween-20 (TBST), incubation was continued with HRPconjugated goat anti-rabbit IgG secondary antibody (1 : 1,000, A0208, Beyotime) for 2 hours at room temperature.

other:

Article Title: LncRNA EGFR-AS1 inhibits ferroptosis to reduce radiosensitivity of cervical cancer through the m6A/IGF2BP3/APAF1 axis.
Article Snippet: Western blot 294 Cervical cancer cells were lysed by RIPA buffer (Thermo), allowing 295 for the extraction of total protein.

Article Title: METTL3-mediated m 6 A modification of CACNA1E promotes osteosarcoma progression and chemoresistance by enhancing WNT7B-mediated Ca 2+ signaling
Article Snippet: Antibodies used were as follows: antibodies of CACNA1E (24697–1-AP), GAPDH (10494–1-AP), IGF2BP1 (22803–1-AP), IGF2BP2 (24744–1-AP), IGF2BP3 (14642–1-AP), YTHDF1 (17479–1-AP), YTHDC1 (14392–1-AP), and FLAG (20543–1-AP) from Proteintech (Shanghai, China); antibodies of N-cadherin (ab76011), Vimentin (ab8069), E-cadherin (ab231303), METTL3 (ab195352), WNT7B (ab227607), and P-gp (ab129450) from Abcam (Cambridge, MA, USA); CAMK2A antibody (#11945) from CST (Danvers, MA, USA); antibodies of CAMK2N (SAB4300530) and PLCB2 (HPA041298) from Sigma-Aldrich (St. Louis, MO, USA); antibody of PLCB4 (PA5-100855) from ThermoFisher.

Image Search Results

Journal: BMC Musculoskeletal Disorders

Article Title: The METTL3-IGF2BP3 axis drives osteosarcoma progression by enhancing ID1 mRNA stability

doi: 10.1186/s12891-026-09527-0

Figure Lengend Snippet: Identification of OS-specific RNA-binding proteins regulating ID1 and mechanistic predictions. A : Volcano plot of DEGs in the GSE253548 dataset. B : Venn diagram intersecting ENCORI-predicted RBPs with DEGs. C : Feature selection by LASSO regression. D : SVM-RFE algorithm-derived feature genes. E : Variable importance ranking from random forest analysis. F : Venn diagram overlapping feature genes identified by LASSO, SVM-RFE, and random forest. G : IGF2BP3 expression profile in the GSE253548 dataset. H : RT-qPCR analysis of IGF2BP3 mRNA levels. I : Western blot detection of IGF2BP3 protein expression. J : SRAMP-predicted m 6 A modification site on ID1 mRNA. K : Secondary structure of the m 6 A-modified site at 782 nt on ID1 mRNA. L : MeRIP-qPCR quantification of ID1 m 6 A modification levels. M : RM2Target-predicted m 6 A-mediated interaction between IGF2BP3 and ID1. N : Spearman correlation of IGF2BP3 and ID1 expression. Data are presented as mean ± SEM. ** P < 0.01, *** P < 0.001, **** P < 0.0001. Statistical analyses were performed using independent-samples t -test or one-way ANOVA followed by Tukey’s post hoc test. All experiments were performed in triplicate.

Article Snippet: Following lysis of infected MG-63 cells, supernatants were incubated overnight at 4 °C with pre-blocked Protein A/G agarose beads conjugated to anti-IGF2BP3 antibody (81805-1-RR, Proteintech), anti-METTL3 (#86132, Cell Signaling Technology, Danvers, MA, USA), or control IgG (A7016, Beyotime).

Techniques: RNA Binding Assay, Selection, Derivative Assay, Expressing, Quantitative RT-PCR, Western Blot, Modification

IGF2BP3 regulates ID1 to modulate malignant behaviors in OS Cells. A - B : RT-qPCR analysis of IGF2BP3 expression in engineered cells. C : RT-qPCR detection of ID1 mRNA levels. D : Western blot analysis of ID1 protein expression. E : RIP-qPCR demonstrating IGF2BP3-ID1 mRNA interaction, with RIP efficiency validated by Western blot. F : Schematic of ID1-WT/MUT CDS and their dual-luciferase reporter constructs. G : Dual-luciferase reporter gene assay results. H : RT-qPCR for ID1 mRNA expression in cells at different time points (0, 2, 4, 6 h) after actinomycin D treatment. I : Western blot for IGF2BP3 and ID1 expression in cells. J : CCK-8 assay assessing cellular proliferation. K : Colony formation capacity quantified across experimental groups. L : Scratch assay evaluating cell migration ability, scale bar = 400 μm. M : Transwell invasion assay with representative images, scale bar = 200 μm. Data are presented as mean ± SEM. ns P > 0.05, **** P < 0.0001. Statistical analyses were performed using independent-samples t-test or one-way ANOVA followed by Tukey’s post hoc test. All experiments were performed in triplicate.

Journal: BMC Musculoskeletal Disorders

Article Title: The METTL3-IGF2BP3 axis drives osteosarcoma progression by enhancing ID1 mRNA stability

doi: 10.1186/s12891-026-09527-0

Figure Lengend Snippet: IGF2BP3 regulates ID1 to modulate malignant behaviors in OS Cells. A - B : RT-qPCR analysis of IGF2BP3 expression in engineered cells. C : RT-qPCR detection of ID1 mRNA levels. D : Western blot analysis of ID1 protein expression. E : RIP-qPCR demonstrating IGF2BP3-ID1 mRNA interaction, with RIP efficiency validated by Western blot. F : Schematic of ID1-WT/MUT CDS and their dual-luciferase reporter constructs. G : Dual-luciferase reporter gene assay results. H : RT-qPCR for ID1 mRNA expression in cells at different time points (0, 2, 4, 6 h) after actinomycin D treatment. I : Western blot for IGF2BP3 and ID1 expression in cells. J : CCK-8 assay assessing cellular proliferation. K : Colony formation capacity quantified across experimental groups. L : Scratch assay evaluating cell migration ability, scale bar = 400 μm. M : Transwell invasion assay with representative images, scale bar = 200 μm. Data are presented as mean ± SEM. ns P > 0.05, **** P < 0.0001. Statistical analyses were performed using independent-samples t-test or one-way ANOVA followed by Tukey’s post hoc test. All experiments were performed in triplicate.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Luciferase, Construct, Reporter Gene Assay, CCK-8 Assay, Wound Healing Assay, Migration, Transwell Invasion Assay

The METTL3-IGF2BP3 axis mediates m 6 A-dependent regulation of ID1 expression and stability. A : METTL3 expression in GSE253548 dataset. B - C : METTL3 expression in cell lines via RT-qPCR (B) and Western blot (C). D : RM2Target prediction of METTL3-ID1 interaction. E : Spearman correlation between METTL3 and ID1 expression. F - G : METTL3 expression validation in engineered cells. H : MeRIP-qPCR analysis of ID1 m6A modification levels. I : RIP-qPCR analysis of the interaction between METTL3 and ID1 mRNA, with the efficiency of the RIP assay validated by Western blot. J : RIP-qPCR detection of IGF2BP3-ID1 mRNA interaction. K : MeRIP-qPCR analysis of ID1 m6A modification levels in MG-63 cells with endogenous METTL3 knockdown. L : RIP-qPCR analysis of the interaction between IGF2BP3 and ID1 mRNA in MG-63 cells with endogenous METTL3 knockdown. M - N : ID1 mRNA (M) and protein (N) expression under indicated treatments. O : RNA stability assay under actinomycin D treatment (0, 2, 4, 6 h). Data are presented as mean ± SEM. ns P > 0.05, *** P < 0.001, **** P < 0.0001. Statistical analyses were performed using independent-samples t-test or one-way ANOVA followed by Tukey’s post hoc test. All experiments were performed in triplicate.

Journal: BMC Musculoskeletal Disorders

Article Title: The METTL3-IGF2BP3 axis drives osteosarcoma progression by enhancing ID1 mRNA stability

doi: 10.1186/s12891-026-09527-0

Figure Lengend Snippet: The METTL3-IGF2BP3 axis mediates m 6 A-dependent regulation of ID1 expression and stability. A : METTL3 expression in GSE253548 dataset. B - C : METTL3 expression in cell lines via RT-qPCR (B) and Western blot (C). D : RM2Target prediction of METTL3-ID1 interaction. E : Spearman correlation between METTL3 and ID1 expression. F - G : METTL3 expression validation in engineered cells. H : MeRIP-qPCR analysis of ID1 m6A modification levels. I : RIP-qPCR analysis of the interaction between METTL3 and ID1 mRNA, with the efficiency of the RIP assay validated by Western blot. J : RIP-qPCR detection of IGF2BP3-ID1 mRNA interaction. K : MeRIP-qPCR analysis of ID1 m6A modification levels in MG-63 cells with endogenous METTL3 knockdown. L : RIP-qPCR analysis of the interaction between IGF2BP3 and ID1 mRNA in MG-63 cells with endogenous METTL3 knockdown. M - N : ID1 mRNA (M) and protein (N) expression under indicated treatments. O : RNA stability assay under actinomycin D treatment (0, 2, 4, 6 h). Data are presented as mean ± SEM. ns P > 0.05, *** P < 0.001, **** P < 0.0001. Statistical analyses were performed using independent-samples t-test or one-way ANOVA followed by Tukey’s post hoc test. All experiments were performed in triplicate.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Biomarker Discovery, Modification, Knockdown, Stability Assay

The METTL3-IGF2BP3 axis mediates ID1-dependent malignant behaviors in OS cells. A : CCK-8 assay assessing cell proliferation. B : Colony formation assay quantifying clonogenic capacity. C : Scratch wound healing assay evaluating migration, scale bar = 400 μm. D : Transwell invasion assay, scale bar = 200 μm. E : Cell proliferation evaluated by CCK8 assay. F : Colony formation assay to assess colony numbers. G : Scratch assay to evaluate the migration ability of cells, scale bar = 400 μm. H : Transwell assay to assess the invasion ability of cells, scale bar = 200 μm. Data are presented as mean ± SEM. ** P < 0.01, *** P < 0.001, **** P < 0.0001. Statistical analyses were performed using independent-samples t-test or one-way ANOVA followed by Tukey’s post hoc test. All experiments were performed in triplicate.

Journal: BMC Musculoskeletal Disorders

Article Title: The METTL3-IGF2BP3 axis drives osteosarcoma progression by enhancing ID1 mRNA stability

doi: 10.1186/s12891-026-09527-0

Figure Lengend Snippet: The METTL3-IGF2BP3 axis mediates ID1-dependent malignant behaviors in OS cells. A : CCK-8 assay assessing cell proliferation. B : Colony formation assay quantifying clonogenic capacity. C : Scratch wound healing assay evaluating migration, scale bar = 400 μm. D : Transwell invasion assay, scale bar = 200 μm. E : Cell proliferation evaluated by CCK8 assay. F : Colony formation assay to assess colony numbers. G : Scratch assay to evaluate the migration ability of cells, scale bar = 400 μm. H : Transwell assay to assess the invasion ability of cells, scale bar = 200 μm. Data are presented as mean ± SEM. ** P < 0.01, *** P < 0.001, **** P < 0.0001. Statistical analyses were performed using independent-samples t-test or one-way ANOVA followed by Tukey’s post hoc test. All experiments were performed in triplicate.

Techniques: CCK-8 Assay, Colony Assay, Wound Healing Assay, Migration, Transwell Invasion Assay, Transwell Assay

Effects of METTL3 and IGF2BP3 inhibitors on ID1-mediated malignant behaviors of OS cells. A : RT-qPCR analysis of ID1 expression in each group of cells. B : WB analysis of ID1 expression in each group of cells. C : RT-qPCR analysis of ID1 mRNA levels at different time points (0, 2, 4, 6 h) following actinomycin D treatment. D : CCK-8 assay assessing cell proliferation. E : Colony formation assay evaluating colony numbers in each group. F : Wound healing assay assessing cell migration ability; scale bar = 400 μm. G : Transwell assay assessing cell invasion ability; scale bar = 200 μm. Data are presented as mean ± SEM. **** P < 0.0001. Statistical analyses were performed using one-way ANOVA followed by Tukey’s post hoc test. All cell experiments were repeated three times.

Journal: BMC Musculoskeletal Disorders

Article Title: The METTL3-IGF2BP3 axis drives osteosarcoma progression by enhancing ID1 mRNA stability

doi: 10.1186/s12891-026-09527-0

Figure Lengend Snippet: Effects of METTL3 and IGF2BP3 inhibitors on ID1-mediated malignant behaviors of OS cells. A : RT-qPCR analysis of ID1 expression in each group of cells. B : WB analysis of ID1 expression in each group of cells. C : RT-qPCR analysis of ID1 mRNA levels at different time points (0, 2, 4, 6 h) following actinomycin D treatment. D : CCK-8 assay assessing cell proliferation. E : Colony formation assay evaluating colony numbers in each group. F : Wound healing assay assessing cell migration ability; scale bar = 400 μm. G : Transwell assay assessing cell invasion ability; scale bar = 200 μm. Data are presented as mean ± SEM. **** P < 0.0001. Statistical analyses were performed using one-way ANOVA followed by Tukey’s post hoc test. All cell experiments were repeated three times.

Techniques: Quantitative RT-PCR, Expressing, CCK-8 Assay, Colony Assay, Wound Healing Assay, Migration, Transwell Assay

The METTL3-IGF2BP3 axis mediates ID1-dependent tumor growth in vivo. A : Western blot analysis of METTL3 and ID1 expression in tumor tissues from each group of mice. B : Western blot analysis of IGF2BP3 and ID1 expression in tumor tissues from each group of mice. C - D : Changes in tumor volume in each group of mice. E - F : Representative images of tumors from each group of mice. G - H : Tumor weights in each group of mice. I - J : Representative immunohistochemical images of Ki67 staining and quantification of Ki67-positive area percentage in tumor tissues from each group of mice, scale bar = 100 μm. N = 6. Data are presented as mean ± SEM. ns P > 0.05, *** P < 0.001, **** P < 0.0001. Statistical analyses were performed using one-way ANOVA followed by Tukey’s post hoc test.

Journal: BMC Musculoskeletal Disorders

Article Title: The METTL3-IGF2BP3 axis drives osteosarcoma progression by enhancing ID1 mRNA stability

doi: 10.1186/s12891-026-09527-0

Figure Lengend Snippet: The METTL3-IGF2BP3 axis mediates ID1-dependent tumor growth in vivo. A : Western blot analysis of METTL3 and ID1 expression in tumor tissues from each group of mice. B : Western blot analysis of IGF2BP3 and ID1 expression in tumor tissues from each group of mice. C - D : Changes in tumor volume in each group of mice. E - F : Representative images of tumors from each group of mice. G - H : Tumor weights in each group of mice. I - J : Representative immunohistochemical images of Ki67 staining and quantification of Ki67-positive area percentage in tumor tissues from each group of mice, scale bar = 100 μm. N = 6. Data are presented as mean ± SEM. ns P > 0.05, *** P < 0.001, **** P < 0.0001. Statistical analyses were performed using one-way ANOVA followed by Tukey’s post hoc test.

Techniques: In Vivo, Western Blot, Expressing, Immunohistochemical staining, Staining

Mechanistic diagram of METTL3-IGF2BP3-mediated ID1 in promoting osteosarcoma progression

Journal: BMC Musculoskeletal Disorders

Article Title: The METTL3-IGF2BP3 axis drives osteosarcoma progression by enhancing ID1 mRNA stability

doi: 10.1186/s12891-026-09527-0

Figure Lengend Snippet: Mechanistic diagram of METTL3-IGF2BP3-mediated ID1 in promoting osteosarcoma progression

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