array ifc (fluidigm)

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fluidigm array ifc
Array Ifc, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 716 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifcs/96%2E96+Dynamic+Array+IFC+for+Gene+Expression/pm41915123-70-9-12
Average 95 stars, based on 716 article reviews

array ifc - by Bioz Stars, 2026-09

95/100 stars

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Software:

Article Title: Genetic bases of resistance to the rice hoja blanca disease deciphered by a quantitative trait locus approach.
Article Snippet: Genetic bases of resistance to RHB disease | 3 D ow nloaded from https://academ ic.oup.com /g3journal/article/13/12/jkad223/7284431 by guest on 09 January 2024 specific PCR that was conducted in 48 × 48 integrated fluidic circuits (IFCs) of the Fluidigm genotyping platform. .. Fluorescent images of the IFCs were acquired in an EP1 reader and analyzed with the Fluidigm Genotyping Analysis Software. ..

Article Title: HBeeID: a molecular tool that identifies honey bee subspecies from different geographic populations.
Article Snippet: Fluidigm’s high-throughput SNP genotyping platform (Juno Genotyping IFC (Integrated Fluidic Circuits) using SNP Type Assays) incorporates pre-amplification and genotyping on integrated IFC’s. .. Sample preparation and pre-amplification is done through thermal cycling and IFCs are transferred to the instruments, BioMark or EP1 reader for capturing fluorescent images, these are then analyzed by the Fluidigm Genotyping Analysis software to generate SNP calls. .. The subset of SNPs, determined to differentiate the 15 HB populations, with 200 SNP-free flanking bases on either side of the SNP loci were sent to the Fluidigm Assay Design Group for the design of SNP primers.

Article Title: HBeeID: a molecular tool that identifies honey bee subspecies from different geographic populations
Article Snippet: Fluidigm’s high-throughput SNP genotyping platform (Juno Genotyping IFC (Integrated Fluidic Circuits) using SNP Type Assays) incorporates pre-amplification and genotyping on integrated IFC’s. .. Sample preparation and pre-amplification is done through thermal cycling and IFCs are transferred to the instruments, BioMark or EP1 reader for capturing fluorescent images, these are then analyzed by the Fluidigm Genotyping Analysis software to generate SNP calls. .. The subset of SNPs, determined to differentiate the 15 HB populations, with 200 SNP-free flanking bases on either side of the SNP loci were sent to the Fluidigm Assay Design Group for the design of SNP primers.

Article Title: Genetic bases of resistance to the rice hoja blanca disease deciphered by a quantitative trait locus approach
Article Snippet: The amplicons were used as the template for allele-specific PCR that was conducted in 48 × 48 integrated fluidic circuits (IFCs) of the Fluidigm genotyping platform. .. Fluorescent images of the IFCs were acquired in an EP1 reader and analyzed with the Fluidigm Genotyping Analysis Software. ..

Binding Assay:

Article Title: Impact of Differentiated Macrophage-Like Cells on the Transcriptional Toxicity Profile of CuO Nanoparticles in Co-Cultured Lung Epithelial Cells
Article Snippet: DNA suspension buffer, PCR certified water, and TE buffer were obtained from Teknova (Hollister, USA). .. The 2× Assay Loading Reagent, 20× DNA Binding Dye, and IFCs were purchased from Fluidigm (San Francisco, USA). .. The 2× SsoFast EvaGreen Supermix was provided by Bio-Rad (Munich, Germany), and the 2× TaqMan PreAmp Master Mix was bought from Applied Biosystems (Darmstadt, Germany).

Control:

Article Title: Absolute Quantification of mRNA Isoforms in Adult Stem Cells Using Microfluidic Digital PCR
Article Snippet: .. GE 48.48 Dynamic Array Reagent kit with control line fluid, 10 IFCs (Fluidigm, catalog number: 85000800) (contains the control line fluid, 20× GE Sample Loading Reagent) 2. .. TE buffer, pH 8.0 (Teknova, catalog number: T0224) 3.

Gene Expression:

Article Title: Characterization of In Vitro 3D Cell Model Developed from Human Hepatocellular Carcinoma (HepG2) Cell Line
Article Snippet: The PreAmp GrandMasterMix (TA05-50) was obtained from TATAA Biocenter AB (Göteborg, Sweden). .. GE 48.48 Dynamic Array Sample & Assay loading Reagent Kit—10 IFCs (85000821) and 48.48 Dynamic Array: Gene expression chip were obtained from Fluidigm (South San Francisco). .. Anti-Ki-67-FITC (130-107-586) antibodies and REA Control (I)-FITC (131-104-611) were obtained from Miltenyi Biotec (Bergisch Gladbach, Germany).

Sample Prep:

other:

Article Title: Therapeutic compounds and uses thereof
Article Snippet: Specific target amplification was performed using 1.25 μl cDNA, Taqman assays (Life Technologies cat #4331182) at a final concentration of 0.2×, and Taqman Preamp Master Mix (Life Technologies cat #4488593) and subsequently diluted according to the protocol for Fluidigm qPCR (Fluidigm Corp).

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