Journal: Open Medicine

Article Title: ARAF regulates malignant progression of bladder cancer through the p38MAPK pathway

doi: 10.1515/med-2026-1422

Figure Lengend Snippet: Expression levels of ARAF in bladder cancer cells. (A) mRNA expression of ARAF in different cell lines. (B and C) Protein expression of ARAF in different cell lines. Data are presented as mean ± SD from three independent biological replicates. Statistical analysis was performed using one-way ANOVA. ***p<0.001, ****p<0.0001 vs. SV-HUC-1.

Article Snippet: The human ureter epithelial immortalized cell line SV-HUC-1 (CL-0222) and human bladder cancer cell lines T24 (CL-0227) and J82 (CL-0125) were purchased from Wuhan Procell Biological Company.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Ribosome biogenesis programs define a three-gene RBscore with prognostic relevance in bladder cancer

doi: 10.3389/fimmu.2026.1810132

Figure Lengend Snippet: Experimental validation of RBscore signature gene expression. (A) RT-qPCR of PIN4, POP4, and PRKDC mRNA in SV-HUC-1 and T24 cells. (B) RT-qPCR of PIN4, POP4, and PRKDC mRNA in paired BLCA and adjacent normal tissues. (C) Western blot analysis and quantification of PIN4, POP4, and PRKDC protein in SV-HUC-1 and T24 cells. (D) Representative IHC images of PRKDC in BLCA and adjacent normal tissues. (E) Quantification of PRKDC IHC scores in BLCA and adjacent normal tissues. * P < 0.05, *** P < 0.001. BLCA, bladder cancer; RBscore, ribosome biogenesis related score; IHC, immunohistochemistry.

Article Snippet: Human bladder urothelial SV-HUC-1 cells (Procell, China) and bladder cancer T24 cells (Procell, China) were cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 1% penicillin–streptomycin at 37 °C in a humidified incubator with 5% CO 2 .

Techniques: Biomarker Discovery, Gene Expression, Quantitative RT-PCR, Western Blot, Immunohistochemistry