Journal: International Journal of Molecular Sciences

Article Title: Reduced IQGAP2 Promotes Bladder Cancer through Regulation of MAPK/ERK Pathway and Cytokines

doi: 10.3390/ijms232113508

Figure Lengend Snippet: Expressions of IQGAP2 in bladder cancer samples and cell lines. ( A ) The transcription level of IQGAP2 was significantly decreased in normal tissue compared to tumor tissue in different types of cancers, including bladder cancer (Oncomine database). ( B ) The expression levels of IQGAP2 in TCGA tumor tissues and their corresponding normal tissues showed reduced expressions in bladder cancer (Timer). ( C ) The expressions of IQGAP2 in cancer cell lines derived from the urinary tract were low compared to other tumor entities (CCLE) (* p < 0.05). ( D ) The mRNA levels of IQGAP2 in bladder cancer cell lines were decreased compared to urothelial cell line SV-HUC-1. Bar chart presents the means of replicates ± SEM from three different experiments; * p ≤ 0.05, ** p ≤ 0.01, and *** p ≤ 0.001; Student’s t -test (two-tailed, unpaired) was used for comparison of means. ( E ) The protein levels of IQGAP2 were also decreased in bladder cancer cell lines compared to the urothelial cell line SV-HUC-1 and was lost in T24.

Article Snippet: The normal urothelium cell line of SV-HUC-1 was purchased from amsbio, Germany.

Techniques: Expressing, Derivative Assay, Two Tailed Test