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htlr5  (InvivoGen)


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    InvivoGen htlr5
    Htlr5, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/htlr5/HEK-Blue+hTLR5+cells/pm41962328-330-12-21
    Average 95 stars, based on 79 article reviews
    htlr5 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Design, synthesis and biological evaluation of novel pyrimidine and quinazoline-based small-molecule Toll-like receptor 8 antagonists.
    Article Snippet: .. Human Embryonic Kidney (HEK)-Blue Null1, HEK-Blue hTLR2TLR1, HEK-Blue hTLR2-TLR6, HEK-Blue hTLR4, HEK-Blue hTLR5, HEKBlue hTLR7, HEK-Blue hTLR8, and HEK-Blue hTLR9 cells (InvivoGen, Toulouse, France) were cultured in Dulbecco's modified Eagle's medium (PAN-Biotech, Aidenbach, Germany) containing 10% (v/v) heat inactivated fetal bovine serum (FBS; S0615), 100 U/mL penicillin, 100 mg/mL streptomycin (P4333), 2 mM L-glutamine (G7513) (all from Sigma Aldrich, Taufkirchen, Germany), 100 μg/mL normocin and selective antibiotics HEK-Blue Selection (hTLR2-TLR1, hTLR2-TLR6, hTLR4), 100 μg/mL zeocin (Null1) and 100 μg/mL zeocin with 10 μg/mL (hTLR7, hTLR9) resp. 30 μg/mL (hTLR5, hTLR8) blasticidin (all from InvivoGen, Toulouse, France). .. THP1-Dual TLR4/MD-2/CD14 cells (InvivoGen, Toulouse, France) were cultured in RPMI-1640 medium supplemented with 10% FBS, penicillin (100U/mL), streptomycin (100 μg/mL), L-glutamine (2 mM), HEPES (25 mM), normocin (100 μg/ mL) and selective antibiotics (blasticidin: 10 μg/mL, zeocin: 100 μg/mL) following the manufacturer's instructions.

    Article Title: Discovery of Novel Isoxazole-Based Small-Molecule Toll-Like Receptor 8 Antagonists.
    Article Snippet: Toll-like receptor 8 (TLR8) recognizes viral and bacterial RNA, initiating inflammatory responses that are crucial for innate immunity.. Dysregulated TLR8 signaling contributes to autoimmune diseases, including systemic lupus erythematosus and rheumatoid arthritis, driving chronic inflammation and tissue damage.. Therefore, targeting TLR8 has gained attention as a promising therapeutic strategy.

    Modification:

    Article Title: Design, synthesis and biological evaluation of novel pyrimidine and quinazoline-based small-molecule Toll-like receptor 8 antagonists.
    Article Snippet: .. Human Embryonic Kidney (HEK)-Blue Null1, HEK-Blue hTLR2TLR1, HEK-Blue hTLR2-TLR6, HEK-Blue hTLR4, HEK-Blue hTLR5, HEKBlue hTLR7, HEK-Blue hTLR8, and HEK-Blue hTLR9 cells (InvivoGen, Toulouse, France) were cultured in Dulbecco's modified Eagle's medium (PAN-Biotech, Aidenbach, Germany) containing 10% (v/v) heat inactivated fetal bovine serum (FBS; S0615), 100 U/mL penicillin, 100 mg/mL streptomycin (P4333), 2 mM L-glutamine (G7513) (all from Sigma Aldrich, Taufkirchen, Germany), 100 μg/mL normocin and selective antibiotics HEK-Blue Selection (hTLR2-TLR1, hTLR2-TLR6, hTLR4), 100 μg/mL zeocin (Null1) and 100 μg/mL zeocin with 10 μg/mL (hTLR7, hTLR9) resp. 30 μg/mL (hTLR5, hTLR8) blasticidin (all from InvivoGen, Toulouse, France). .. THP1-Dual TLR4/MD-2/CD14 cells (InvivoGen, Toulouse, France) were cultured in RPMI-1640 medium supplemented with 10% FBS, penicillin (100U/mL), streptomycin (100 μg/mL), L-glutamine (2 mM), HEPES (25 mM), normocin (100 μg/ mL) and selective antibiotics (blasticidin: 10 μg/mL, zeocin: 100 μg/mL) following the manufacturer's instructions.

    Article Title: Discovery of Novel Isoxazole-Based Small-Molecule Toll-Like Receptor 8 Antagonists.
    Article Snippet: Toll-like receptor 8 (TLR8) recognizes viral and bacterial RNA, initiating inflammatory responses that are crucial for innate immunity.. Dysregulated TLR8 signaling contributes to autoimmune diseases, including systemic lupus erythematosus and rheumatoid arthritis, driving chronic inflammation and tissue damage.. Therefore, targeting TLR8 has gained attention as a promising therapeutic strategy.

    Selection:

    Article Title: Design, synthesis and biological evaluation of novel pyrimidine and quinazoline-based small-molecule Toll-like receptor 8 antagonists.
    Article Snippet: .. Human Embryonic Kidney (HEK)-Blue Null1, HEK-Blue hTLR2TLR1, HEK-Blue hTLR2-TLR6, HEK-Blue hTLR4, HEK-Blue hTLR5, HEKBlue hTLR7, HEK-Blue hTLR8, and HEK-Blue hTLR9 cells (InvivoGen, Toulouse, France) were cultured in Dulbecco's modified Eagle's medium (PAN-Biotech, Aidenbach, Germany) containing 10% (v/v) heat inactivated fetal bovine serum (FBS; S0615), 100 U/mL penicillin, 100 mg/mL streptomycin (P4333), 2 mM L-glutamine (G7513) (all from Sigma Aldrich, Taufkirchen, Germany), 100 μg/mL normocin and selective antibiotics HEK-Blue Selection (hTLR2-TLR1, hTLR2-TLR6, hTLR4), 100 μg/mL zeocin (Null1) and 100 μg/mL zeocin with 10 μg/mL (hTLR7, hTLR9) resp. 30 μg/mL (hTLR5, hTLR8) blasticidin (all from InvivoGen, Toulouse, France). .. THP1-Dual TLR4/MD-2/CD14 cells (InvivoGen, Toulouse, France) were cultured in RPMI-1640 medium supplemented with 10% FBS, penicillin (100U/mL), streptomycin (100 μg/mL), L-glutamine (2 mM), HEPES (25 mM), normocin (100 μg/ mL) and selective antibiotics (blasticidin: 10 μg/mL, zeocin: 100 μg/mL) following the manufacturer's instructions.

    Article Title: Discovery of Novel Isoxazole-Based Small-Molecule Toll-Like Receptor 8 Antagonists.
    Article Snippet: Toll-like receptor 8 (TLR8) recognizes viral and bacterial RNA, initiating inflammatory responses that are crucial for innate immunity.. Dysregulated TLR8 signaling contributes to autoimmune diseases, including systemic lupus erythematosus and rheumatoid arthritis, driving chronic inflammation and tissue damage.. Therefore, targeting TLR8 has gained attention as a promising therapeutic strategy.

    Activity Assay:

    Article Title: Massively Parallel Screening of Toll/Interleukin-1 Receptor (TIR)-Derived Peptides Reveals Multiple Toll-Like Receptors (TLRs)-Targeting Immunomodulatory Peptides.
    Article Snippet: The absorbance was measured at 450 nm using the Multiskan SkyHigh Spectrophotometer (ThermoFisher Scientific). .. Secreted Embryonic Alkaline Phosphatase (SEAP) Activity Assay: HEKBlueTM hTLR2/1, hTLR3, hTLR4, hTLR5, hTLR7, hTLR8, and hTLR9 (InvivoGen) cells were seeded in a 96-well plate (Costar) at a density of 1 × 105 cells per well. ..



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    A The vector map of recombinant protein constructs: FMS-like tyrosine kinase-3 ligand extracellular domain (Flt3L/F), Vibrio vulnificus flagellin B (FlaB/B). The fusion protein was generated by combining F and B with B position at the C-terminal (FB) or the N-terminal (BF). B Characterization of the recombinant proteins by sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) and subsequent Western blot analysis using mouse anti-FlaB serum or rabbit anti-Flt3L antibody. C Determination of TLR5-dependent NF-κB stimulating activity of B, FB, and BF in various protein concentrations using HEK-Blue™ hTLR5 cells ( n = 3 biological replicates). D Total cell number and the number of cell cluster formations of BMDCs after 9 days of cultures supplemented with B, F, and FB. Floating cells were collected and counted using a Vi-Cell Blue machine ( n = 7 biological replicates), while cell clusters were visualized with an EMOS5000 machine ( n = 5 biological replicates). E Representative flow histogram of CD103 expression on CD11c + MHCII hi population and quantification data of CD103 + DCs generation by recombination protein in a dose-dependent manner using BMDCs ( n = 3 biological replicates). F Representative levels of CFSE in CD8 + T cells from cultures with the indicated DCs, the individual peaks of CFSE dilution gated, and quantitative proliferation index of CD8 + T cell. For all sections: **** P < 0.0001; *** P < 0.001; ** P < 0.01; * P < 0.05.

    Journal: NPJ Vaccines

    Article Title: Antigen cross-presentation potentiating cancer vaccine adjuvant for T cell expansion and synergy with anti-PD-1

    doi: 10.1038/s41541-026-01376-1

    Figure Lengend Snippet: A The vector map of recombinant protein constructs: FMS-like tyrosine kinase-3 ligand extracellular domain (Flt3L/F), Vibrio vulnificus flagellin B (FlaB/B). The fusion protein was generated by combining F and B with B position at the C-terminal (FB) or the N-terminal (BF). B Characterization of the recombinant proteins by sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) and subsequent Western blot analysis using mouse anti-FlaB serum or rabbit anti-Flt3L antibody. C Determination of TLR5-dependent NF-κB stimulating activity of B, FB, and BF in various protein concentrations using HEK-Blue™ hTLR5 cells ( n = 3 biological replicates). D Total cell number and the number of cell cluster formations of BMDCs after 9 days of cultures supplemented with B, F, and FB. Floating cells were collected and counted using a Vi-Cell Blue machine ( n = 7 biological replicates), while cell clusters were visualized with an EMOS5000 machine ( n = 5 biological replicates). E Representative flow histogram of CD103 expression on CD11c + MHCII hi population and quantification data of CD103 + DCs generation by recombination protein in a dose-dependent manner using BMDCs ( n = 3 biological replicates). F Representative levels of CFSE in CD8 + T cells from cultures with the indicated DCs, the individual peaks of CFSE dilution gated, and quantitative proliferation index of CD8 + T cell. For all sections: **** P < 0.0001; *** P < 0.001; ** P < 0.01; * P < 0.05.

    Article Snippet: To assess the TLR5-stimulating activities of the recombinant fusion proteins, we evaluated the TLR5-dependent NF-κB-stimulating activity of the recombinant protein using HEK-BlueTM hTLR5 cells (hκb-help-5, InvivoGen, USA) and HEK-BlueTM Detection (hb-det2, InvivoGen, USA) assay systems following the manufacturer’s instructions.

    Techniques: Plasmid Preparation, Recombinant, Construct, Generated, SDS Page, Western Blot, Activity Assay, Expressing