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human telomerase reverse transcriptase  (ATCC)


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    ATCC human telomerase reverse transcriptase
    Human Telomerase Reverse Transcriptase, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/htert/hTERT+Lung+Fibroblast/pm42864328-234-10-26
    Average 94 stars, based on 30 article reviews
    human telomerase reverse transcriptase - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    In Vitro:

    Article Title: Different kinetics of humoral response against individual antigens in the ovine model of Toxoplasma gondii infection and its pathological association
    Article Snippet: .. RH RHΔhxgprt ( ) and Me49 ( ) T. gondii strains were grown in standard tachyzoite conditions in vitro : hTERT (ATCC ® CRL-4001, USA) monolayers were infected with tachyzoites and incubated with Dulbecco’s modified Eagle medium (DMEM, Invitrogen) supplemented with 1% fetal bovine serum (FBS, Internegocios S.A., Argentina) and penicillin (10,000 units/ml)-streptomycin (10 mg/ml) solution (Gibco, Argentina) at 37°C and 5% CO 2 . ..

    Article Title: Different kinetics of humoral response against individual antigens in the ovine model of Toxoplasma gondii infection and its pathological association
    Article Snippet: .. RH RHDhxgprt (26) and Me49 (23) T. gondii strains were grown in standard tachyzoite conditions in vitro: hTERT (ATCC® CRL-4001, USA) monolayers were infected with tachyzoites and incubated with Dulbecco’s modified Eagle medium (DMEM, Invitrogen) supplemented with 1% fetal bovine serum (FBS, Internegocios S.A., Argentina) and penicillin (10,000 units/ml)streptomycin (10 mg/ml) solution (Gibco, Argentina) at 37°C and 5% CO2. ..

    Infection:

    Article Title: Different kinetics of humoral response against individual antigens in the ovine model of Toxoplasma gondii infection and its pathological association
    Article Snippet: .. RH RHΔhxgprt ( ) and Me49 ( ) T. gondii strains were grown in standard tachyzoite conditions in vitro : hTERT (ATCC ® CRL-4001, USA) monolayers were infected with tachyzoites and incubated with Dulbecco’s modified Eagle medium (DMEM, Invitrogen) supplemented with 1% fetal bovine serum (FBS, Internegocios S.A., Argentina) and penicillin (10,000 units/ml)-streptomycin (10 mg/ml) solution (Gibco, Argentina) at 37°C and 5% CO 2 . ..

    Article Title: Different kinetics of humoral response against individual antigens in the ovine model of Toxoplasma gondii infection and its pathological association
    Article Snippet: .. RH RHDhxgprt (26) and Me49 (23) T. gondii strains were grown in standard tachyzoite conditions in vitro: hTERT (ATCC® CRL-4001, USA) monolayers were infected with tachyzoites and incubated with Dulbecco’s modified Eagle medium (DMEM, Invitrogen) supplemented with 1% fetal bovine serum (FBS, Internegocios S.A., Argentina) and penicillin (10,000 units/ml)streptomycin (10 mg/ml) solution (Gibco, Argentina) at 37°C and 5% CO2. ..

    Incubation:

    Article Title: Different kinetics of humoral response against individual antigens in the ovine model of Toxoplasma gondii infection and its pathological association
    Article Snippet: .. RH RHΔhxgprt ( ) and Me49 ( ) T. gondii strains were grown in standard tachyzoite conditions in vitro : hTERT (ATCC ® CRL-4001, USA) monolayers were infected with tachyzoites and incubated with Dulbecco’s modified Eagle medium (DMEM, Invitrogen) supplemented with 1% fetal bovine serum (FBS, Internegocios S.A., Argentina) and penicillin (10,000 units/ml)-streptomycin (10 mg/ml) solution (Gibco, Argentina) at 37°C and 5% CO 2 . ..

    Article Title: Different kinetics of humoral response against individual antigens in the ovine model of Toxoplasma gondii infection and its pathological association
    Article Snippet: .. RH RHDhxgprt (26) and Me49 (23) T. gondii strains were grown in standard tachyzoite conditions in vitro: hTERT (ATCC® CRL-4001, USA) monolayers were infected with tachyzoites and incubated with Dulbecco’s modified Eagle medium (DMEM, Invitrogen) supplemented with 1% fetal bovine serum (FBS, Internegocios S.A., Argentina) and penicillin (10,000 units/ml)streptomycin (10 mg/ml) solution (Gibco, Argentina) at 37°C and 5% CO2. ..

    Modification:

    Article Title: Different kinetics of humoral response against individual antigens in the ovine model of Toxoplasma gondii infection and its pathological association
    Article Snippet: .. RH RHΔhxgprt ( ) and Me49 ( ) T. gondii strains were grown in standard tachyzoite conditions in vitro : hTERT (ATCC ® CRL-4001, USA) monolayers were infected with tachyzoites and incubated with Dulbecco’s modified Eagle medium (DMEM, Invitrogen) supplemented with 1% fetal bovine serum (FBS, Internegocios S.A., Argentina) and penicillin (10,000 units/ml)-streptomycin (10 mg/ml) solution (Gibco, Argentina) at 37°C and 5% CO 2 . ..

    Article Title: Different kinetics of humoral response against individual antigens in the ovine model of Toxoplasma gondii infection and its pathological association
    Article Snippet: .. RH RHDhxgprt (26) and Me49 (23) T. gondii strains were grown in standard tachyzoite conditions in vitro: hTERT (ATCC® CRL-4001, USA) monolayers were infected with tachyzoites and incubated with Dulbecco’s modified Eagle medium (DMEM, Invitrogen) supplemented with 1% fetal bovine serum (FBS, Internegocios S.A., Argentina) and penicillin (10,000 units/ml)streptomycin (10 mg/ml) solution (Gibco, Argentina) at 37°C and 5% CO2. ..

    Article Title: TOP2B modulates DNA supercoiling and chromatin contacts during transcriptional induction
    Article Snippet: .. MCF7 cells (European Collection of Authenticated Cell Cultures, 86012803) were cultured in minimum essential medium (MEM) with Earle’s salts supplemented with penicillin (50 U/ml), streptomycin (50 μg/ml), 2 mM l -glutamine, 10% fetal bovine serum (FBS; Sigma-Aldrich), and nonessential amino acids. hTERT–RPE-1 cells (American Type Culture Collection, CRL-4000) were culture in Dulbecco’s modified Eagle’s medium F12 supplemented with penicillin (50 U/ml), streptomycin (50 μg/ml), 2 mM l -glutamine, and 10% FBS (Sigma-Aldrich). .. Cell maintenance was conducted in HEPA class-100 incubators (Thermo Fisher Scientific) at 37°C with 5% CO 2 .

    Article Title: Centromeric α-satellite DNA is a hotspot of genotoxic damage, incomplete repair, and cytoplasmic mislocalization
    Article Snippet: .. hTERT-immortalized human fibroblasts (CHON-002, ATCC CRL-2847; BJ-5ta, ATCC CRL-4001) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin-streptomycin (Gibco). ..

    Cell Culture:

    Article Title: TOP2B modulates DNA supercoiling and chromatin contacts during transcriptional induction
    Article Snippet: .. MCF7 cells (European Collection of Authenticated Cell Cultures, 86012803) were cultured in minimum essential medium (MEM) with Earle’s salts supplemented with penicillin (50 U/ml), streptomycin (50 μg/ml), 2 mM l -glutamine, 10% fetal bovine serum (FBS; Sigma-Aldrich), and nonessential amino acids. hTERT–RPE-1 cells (American Type Culture Collection, CRL-4000) were culture in Dulbecco’s modified Eagle’s medium F12 supplemented with penicillin (50 U/ml), streptomycin (50 μg/ml), 2 mM l -glutamine, and 10% FBS (Sigma-Aldrich). .. Cell maintenance was conducted in HEPA class-100 incubators (Thermo Fisher Scientific) at 37°C with 5% CO 2 .

    Article Title: Centromeric α-satellite DNA is a hotspot of genotoxic damage, incomplete repair, and cytoplasmic mislocalization
    Article Snippet: .. hTERT-immortalized human fibroblasts (CHON-002, ATCC CRL-2847; BJ-5ta, ATCC CRL-4001) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin-streptomycin (Gibco). ..

    other:

    Article Title: hTERT-immortalized mesenchymal stem cell-derived EV treatment reduces ZIKV-induced cortical neuronal death, infection, and exosome-mediated transmission.
    Article Snippet: The hTERT-immortalized mesenchymal stem cell-derived EVs (product “ATCC SCRC-4000-EXM”) were obtained in four vials (100 μL each) and were aliquoted at ~616 μg of protein per vial.



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    Image Search Results


    hTERT protects against endothelial dysfunction. (A) Enlarged images of DiI-oxLDL in HUVEC cells under hTERT overexpression (hTERT Oe) and hTERT knockdown (hTERT Kd) conditions. Vec Control represents respective vector controls. Corresponding full size raw images are given in . (B) Quantification of DiI-oxLDL fluorescence intensity under different conditions. (C) Calcein stained THP1 monocyte (shown in green by arrows) attached to HUVEC cells transfected with hTERT overexpression and hTERT knockdown plasmids. (D) Quantification of cell counts for monocyte attachment assay. (E) Trans endothelial resistance (TEER) measured from HUVEC cells grown on transwell plates using voltohm meter. (F) Barrier breach quantification performed using HUVEC cells cultured on transwell plates using 40 kDa FITC dextran. (G) Enlarged Immunofluorescence images showing HUVEC cells expressing ZO-1, white arrows indicate tight junctions. Corresponding full size raw images are given in (H) ZO-1 quantification. (I) Schema showing rescue effects of hTERT (Created using BioRender.com). All data are representative of n = 3 biological replicates. Data are presented as Mean ± SEM. Scale bars =20 µm (A) , 400 µm (C) , 100 µm (G) Unpaired two-tailed t-test, * p < 0.05, ** p < 0.005, *** p < 0.0005, **** p < 0.00005.

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: hTERT associates with Rab7A to enhance autophagy and rescue oxLDL induced endothelial dysfunction

    doi: 10.3389/fcvm.2026.1882233

    Figure Lengend Snippet: hTERT protects against endothelial dysfunction. (A) Enlarged images of DiI-oxLDL in HUVEC cells under hTERT overexpression (hTERT Oe) and hTERT knockdown (hTERT Kd) conditions. Vec Control represents respective vector controls. Corresponding full size raw images are given in . (B) Quantification of DiI-oxLDL fluorescence intensity under different conditions. (C) Calcein stained THP1 monocyte (shown in green by arrows) attached to HUVEC cells transfected with hTERT overexpression and hTERT knockdown plasmids. (D) Quantification of cell counts for monocyte attachment assay. (E) Trans endothelial resistance (TEER) measured from HUVEC cells grown on transwell plates using voltohm meter. (F) Barrier breach quantification performed using HUVEC cells cultured on transwell plates using 40 kDa FITC dextran. (G) Enlarged Immunofluorescence images showing HUVEC cells expressing ZO-1, white arrows indicate tight junctions. Corresponding full size raw images are given in (H) ZO-1 quantification. (I) Schema showing rescue effects of hTERT (Created using BioRender.com). All data are representative of n = 3 biological replicates. Data are presented as Mean ± SEM. Scale bars =20 µm (A) , 400 µm (C) , 100 µm (G) Unpaired two-tailed t-test, * p < 0.05, ** p < 0.005, *** p < 0.0005, **** p < 0.00005.

    Article Snippet: 2 μg of plasmid DNA – pWZL-hTERT for overexpression (A kind gift from William Hahn, Addgene plasmid # 22396) transEDIT pCLIP-gRNA-hTERT for hTERT knockdown along with respective vector controls were used for transfection.

    Techniques: Over Expression, Knockdown, Control, Plasmid Preparation, Fluorescence, Staining, Transfection, Cell Culture, Immunofluorescence, Expressing, Two Tailed Test

    hTERT regulates autophagy kinetics. (A–D) Western blots showing expression of autophagy related proteins under 24 h of 100 mM Trehalose (activator) and 5 µM chloroquine (inhibitor) treatment in HUVEC cells with hTERT overexpression (hTERT Oe) and hTERT knockdown (hTERT Kd). Vec control represents respective vector controls used in transfection. Data are representative of three independent replicates. (E,G) Fluorescent images of HUVEC cells transfected with mCherry-GFP-LC3 dual reporter plasmid under different conditions and quantification of yellow and red puncta (as indicated by colored bars) to assess autophagy flux. (F,H) Live cell imaging of lysosomes in HUVEC cells using Nanolive™ 3D cell explorer and quantifications across different time points. All data are representative of n = 3 biological replicates. Unpaired two-tailed t-test ** p < 0.005, **** p < 0.00005. Data are presented as Mean ± SEM, Scale bar =75 µm (E) Scale bar =20 µm (F) .

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: hTERT associates with Rab7A to enhance autophagy and rescue oxLDL induced endothelial dysfunction

    doi: 10.3389/fcvm.2026.1882233

    Figure Lengend Snippet: hTERT regulates autophagy kinetics. (A–D) Western blots showing expression of autophagy related proteins under 24 h of 100 mM Trehalose (activator) and 5 µM chloroquine (inhibitor) treatment in HUVEC cells with hTERT overexpression (hTERT Oe) and hTERT knockdown (hTERT Kd). Vec control represents respective vector controls used in transfection. Data are representative of three independent replicates. (E,G) Fluorescent images of HUVEC cells transfected with mCherry-GFP-LC3 dual reporter plasmid under different conditions and quantification of yellow and red puncta (as indicated by colored bars) to assess autophagy flux. (F,H) Live cell imaging of lysosomes in HUVEC cells using Nanolive™ 3D cell explorer and quantifications across different time points. All data are representative of n = 3 biological replicates. Unpaired two-tailed t-test ** p < 0.005, **** p < 0.00005. Data are presented as Mean ± SEM, Scale bar =75 µm (E) Scale bar =20 µm (F) .

    Article Snippet: 2 μg of plasmid DNA – pWZL-hTERT for overexpression (A kind gift from William Hahn, Addgene plasmid # 22396) transEDIT pCLIP-gRNA-hTERT for hTERT knockdown along with respective vector controls were used for transfection.

    Techniques: Western Blot, Expressing, Over Expression, Knockdown, Control, Plasmid Preparation, Transfection, Live Cell Imaging, Two Tailed Test

    hTERT associates with Rab7A to regulate autophagy. (A) Immunofluorescence images showing expression of hTERT and Rab7A under 40 µg/mL oxLDL treatment of HUVEC cells (B) Enlarged images showing colocalization of hTERT and Rab7A upon oxLDL treatment indicated by arrows. (C) Graph indicates quantification of colocalization using Pearson's correlation plugin in Fiji software. (D,E) Co-immunoprecipitation experiments showing association of hTERT with autophagy related proteins at basal (D) oxLDL treated condition (E,F) Densitometric analysis of hTERT and Rab7A shown in western blots in (E) (G) Enlarged images of DiI-oxLDL in HUVEC cells under Rab7DN, hTERT overexpression (hTERT Oe) and hTERT knockdown (hTERT Kd) conditions. (H) Quantification of DiI-oxLDL fluorescence intensity under different conditions (I) Schema showing possible association step of hTERT in autophagy regulation (Created using BioRender.com). Scale bar=100 µm (A) , 50 µm (B,F) , Data are representative of n = 3 biological replicates, Data are presented as Mean ± SEM, Unpaired two-tailed t-test, * p < 0.05, ** p < 0.005, *** p < 0.0005.

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: hTERT associates with Rab7A to enhance autophagy and rescue oxLDL induced endothelial dysfunction

    doi: 10.3389/fcvm.2026.1882233

    Figure Lengend Snippet: hTERT associates with Rab7A to regulate autophagy. (A) Immunofluorescence images showing expression of hTERT and Rab7A under 40 µg/mL oxLDL treatment of HUVEC cells (B) Enlarged images showing colocalization of hTERT and Rab7A upon oxLDL treatment indicated by arrows. (C) Graph indicates quantification of colocalization using Pearson's correlation plugin in Fiji software. (D,E) Co-immunoprecipitation experiments showing association of hTERT with autophagy related proteins at basal (D) oxLDL treated condition (E,F) Densitometric analysis of hTERT and Rab7A shown in western blots in (E) (G) Enlarged images of DiI-oxLDL in HUVEC cells under Rab7DN, hTERT overexpression (hTERT Oe) and hTERT knockdown (hTERT Kd) conditions. (H) Quantification of DiI-oxLDL fluorescence intensity under different conditions (I) Schema showing possible association step of hTERT in autophagy regulation (Created using BioRender.com). Scale bar=100 µm (A) , 50 µm (B,F) , Data are representative of n = 3 biological replicates, Data are presented as Mean ± SEM, Unpaired two-tailed t-test, * p < 0.05, ** p < 0.005, *** p < 0.0005.

    Article Snippet: 2 μg of plasmid DNA – pWZL-hTERT for overexpression (A kind gift from William Hahn, Addgene plasmid # 22396) transEDIT pCLIP-gRNA-hTERT for hTERT knockdown along with respective vector controls were used for transfection.

    Techniques: Immunofluorescence, Expressing, Software, Co-Immunoprecipitation Assay, Analysis, Western Blot, Over Expression, Knockdown, Fluorescence, Two Tailed Test