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hs578t  (ATCC)


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    Structured Review

    ATCC hs578t
    Hs578t, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 2336 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hs578t/Hs+578T/pm42882828-46-7-12
    Average 97 stars, based on 2336 article reviews
    hs578t - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Multiple Displacement Amplification:

    Article Title: Comprehensive multi-omics landscape exploration and functional characterization of TACSTD2 throughout pan-cancer
    Article Snippet: .. Cell lines and cultures Human breast cancer cell lines, including MCF‐10A, MDA‐MB‐231, BT549, MDA‐MB‐468, HS578T and MDA‐MB‐453, were obtained from the American Type Culture Collection. ..

    Article Title: Soluble N-terminal EphA2 fragment as a diagnostic biomarker and functional mediator of tumor progression in triple-negative breast cancer.
    Article Snippet: .. TNBC cell lines MDA-MB-231, BT549, Hs578T, and MDA-MB-468, along with other breast cancer cell lines and the non-tumorigenic mammary epithelial cell line 184B5, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. All cell lines were maintained under standard culture conditions recommended by ATCC and incubated at 37 ◦C in a humidified incubator with 5% CO2.

    Article Title: Squamous-state excursions activate APOBEC3A in cancer
    Article Snippet: .. For pan-cell line analysis of APOBEC3A and APOBEC3B expression, AU565, BT20,BT-474, Cama1, DU4475, HCC1395, HCC1428, HCC1500, HCC1599, HCC1806, HCC1937, HCC1954, HCC202, HCC2157, HCC2218, HCC38, HCC70, Hs578T, MCF10A, MCF10F, MCF12A, MCF7, MDA-MB-157, MDA-MB-175-VII, MDA-MB-231, MDA-MB-361, MDA-MB-415, MDA-MB-436, MDA-MB-453, MDA-MB-468, SKBR3, T47D, UACC893, and Zr-75-30 cells were obtained from ATCC and cultured according to ATCC recommendations. .. Poly(I:C) (200 ng/μL) was transfected using Lipofectamine 3000 (Invitrogen, L3000015) 24 hours before flow cytometry.

    Article Title: The cellular stress sensor HSPB1 regulates the membrane localization of amino acid transporter SLC7A5 in breast cancer.
    Article Snippet: .. MCF-7, T47-D, ZR-75-1, BT-474, AU565, HCC1569, MDA-MB 231, Hs578T, BT-549, and HEK293T cell lines were obtained from ATCC. .. MCF-7 cells were cultured in EMEM (Nacalai) supplemented with 10% FBS, 10 g/ml insulin, 1 mM sodium pyruvate, and penicillin- streptomycin (Nacalai).

    Article Title: A phenothiazine analog, PTF, suppresses breast cancer growth by inhibiting YAP signaling.
    Article Snippet: Cycloheximide, MG132, chloroquine (CQ), 3-methyladenine, and staurosporine were purchased from Sigma-Aldrich (St. Louis, MO, USA). .. The breast cancer cell lines MCF-7, MDA-MB-231, and Hs578T were originally established and distributed by the American Type Culture Collection (Manassas, VA, USA). .. These cells were expanded in DMEM/ F12 medium (Invitrogen, Carlsbad, CA, USA) formulated with 10 % fetal bovine serum (Gibco, Grand Island, NY, USA).

    Article Title: GAS5 Downregulation Promotes the Progression of Triple-Negative Breast Cancer by Regulating Tumor Cells-MDSCs Crosstalk.
    Article Snippet: .. TNBC cell lines MDA-MB-231, HCC1937, MDA-MB-468, and Hs578T were obtained from the American Type Culture Collection (ATCC). .. Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) and 1% penicillin-streptomycin in a 37 ◦C incubator with 5% CO2.

    Article Title: MPP8 Facilitates DNA DSB Repair via NHEJ to Promote Chemoresistance of TNBC Independent of Methyl-H3K9 Binding.
    Article Snippet: .. Experimental Section .1 Cell Lines, Antibodies, and Inhibitors DA-MD-231, Hs578T, MDA-MB-468, MCF7, U-2 OS, HeLa-S3, 93T (ATCC), as well as MDA-MB-231-D3H2LN (Xenogen) and HEJ and HR reporter 293–140c and 293–97A [ 23 ] cells were ultured in DMEM with 10% FBS and penicillin/streptomycin Corning). .. MPP8 antibody was affinity-purified from rabbit erum against purified antigens (1-188aa) [ 8 ] while other antiodies were from commercial sources, including Cell Signaling PARP1, cleaved Caspase3, γH2AX, KU70, KU80, Lamin B, LIG4, LF), Abcam (Ki67, LDHB), Sigma (Actin, Flag-M2), Santa Cruz XLF, HA), Proteintech (GAPDH, XRCC4), Upstate ( γH2AX), eneTex (LIG4) and JacksonImmuno (IgG and secondary antiodies).

    Expressing:

    Article Title: Squamous-state excursions activate APOBEC3A in cancer
    Article Snippet: .. For pan-cell line analysis of APOBEC3A and APOBEC3B expression, AU565, BT20,BT-474, Cama1, DU4475, HCC1395, HCC1428, HCC1500, HCC1599, HCC1806, HCC1937, HCC1954, HCC202, HCC2157, HCC2218, HCC38, HCC70, Hs578T, MCF10A, MCF10F, MCF12A, MCF7, MDA-MB-157, MDA-MB-175-VII, MDA-MB-231, MDA-MB-361, MDA-MB-415, MDA-MB-436, MDA-MB-453, MDA-MB-468, SKBR3, T47D, UACC893, and Zr-75-30 cells were obtained from ATCC and cultured according to ATCC recommendations. .. Poly(I:C) (200 ng/μL) was transfected using Lipofectamine 3000 (Invitrogen, L3000015) 24 hours before flow cytometry.

    Cell Culture:

    Article Title: Squamous-state excursions activate APOBEC3A in cancer
    Article Snippet: .. For pan-cell line analysis of APOBEC3A and APOBEC3B expression, AU565, BT20,BT-474, Cama1, DU4475, HCC1395, HCC1428, HCC1500, HCC1599, HCC1806, HCC1937, HCC1954, HCC202, HCC2157, HCC2218, HCC38, HCC70, Hs578T, MCF10A, MCF10F, MCF12A, MCF7, MDA-MB-157, MDA-MB-175-VII, MDA-MB-231, MDA-MB-361, MDA-MB-415, MDA-MB-436, MDA-MB-453, MDA-MB-468, SKBR3, T47D, UACC893, and Zr-75-30 cells were obtained from ATCC and cultured according to ATCC recommendations. .. Poly(I:C) (200 ng/μL) was transfected using Lipofectamine 3000 (Invitrogen, L3000015) 24 hours before flow cytometry.



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    CEP exerts cytotoxicity in TNBC cells. (A) Chemical structure of CEP. (B) CEP inhibited TNBC cell viability. The effect of CEP treatment on TNBC cell viability after 24, 48 and 72 h. (C) CEP inhibited clonogenicity of MDA-MB-231 and <t>Hs578T</t> cells. n=3; *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control. CEP, cepharanthine; TNBC, triple-negative breast cancer.
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    human breast cancer cells hs578t  (ATCC)
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    Cell viability (MTT assay) of HUVEC (A) and <t>HS578T</t> cells (B) treated for 48 h with commercial DTX or the DTX:γ-CD complex. Results expressed as mean ± SD ( n = 6). Statistical analysis by two-way ANOVA plus Tukey–Kramer post hoc: * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001.
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    Cell viability (MTT assay) of HUVEC (A) and <t>HS578T</t> cells (B) treated for 48 h with commercial DTX or the DTX:γ-CD complex. Results expressed as mean ± SD ( n = 6). Statistical analysis by two-way ANOVA plus Tukey–Kramer post hoc: * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001.
    Hs578t Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    CEP exerts cytotoxicity in TNBC cells. (A) Chemical structure of CEP. (B) CEP inhibited TNBC cell viability. The effect of CEP treatment on TNBC cell viability after 24, 48 and 72 h. (C) CEP inhibited clonogenicity of MDA-MB-231 and Hs578T cells. n=3; *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control. CEP, cepharanthine; TNBC, triple-negative breast cancer.

    Journal: Molecular Medicine Reports

    Article Title: Cepharanthine inhibits lysosomes and induces apoptosis in triple-negative breast cancer cells

    doi: 10.3892/mmr.2026.13899

    Figure Lengend Snippet: CEP exerts cytotoxicity in TNBC cells. (A) Chemical structure of CEP. (B) CEP inhibited TNBC cell viability. The effect of CEP treatment on TNBC cell viability after 24, 48 and 72 h. (C) CEP inhibited clonogenicity of MDA-MB-231 and Hs578T cells. n=3; *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control. CEP, cepharanthine; TNBC, triple-negative breast cancer.

    Article Snippet: The TNBC cell lines MDA-MB-231 (cat. no. CL-0150; Procell Life Science & Technology Co., Ltd.) and Hs578T (cat. no. CL-0114; Procell Life Science & Technology Co., Ltd.) were cultured in high-glucose DMEM medium (cat. no. C11965500BT; Gibco; Thermo Fisher Scientific, Inc.), supplemented with 10% fetal bovine serum (cat. no. 164210; Procell Life Science & Technology Co., Ltd.) and 1% penicillin/streptomycin (cat. no. 15140122; Gibco; Thermo Fisher Scientific, Inc.).

    Techniques: Control

    CEP induces apoptosis in triple-negative breast cancer cells. Flow cytometric quantification of apoptosis induced by CEP in (A) MDA-MB-231 and (B) Hs578T cells (n=3; 48 h). **P<0.01 and ***P < 0.001 vs. control. CEP, cepharanthine; ns, not significant.

    Journal: Molecular Medicine Reports

    Article Title: Cepharanthine inhibits lysosomes and induces apoptosis in triple-negative breast cancer cells

    doi: 10.3892/mmr.2026.13899

    Figure Lengend Snippet: CEP induces apoptosis in triple-negative breast cancer cells. Flow cytometric quantification of apoptosis induced by CEP in (A) MDA-MB-231 and (B) Hs578T cells (n=3; 48 h). **P<0.01 and ***P < 0.001 vs. control. CEP, cepharanthine; ns, not significant.

    Article Snippet: The TNBC cell lines MDA-MB-231 (cat. no. CL-0150; Procell Life Science & Technology Co., Ltd.) and Hs578T (cat. no. CL-0114; Procell Life Science & Technology Co., Ltd.) were cultured in high-glucose DMEM medium (cat. no. C11965500BT; Gibco; Thermo Fisher Scientific, Inc.), supplemented with 10% fetal bovine serum (cat. no. 164210; Procell Life Science & Technology Co., Ltd.) and 1% penicillin/streptomycin (cat. no. 15140122; Gibco; Thermo Fisher Scientific, Inc.).

    Techniques: Control

    CEP induces ΔΨm loss in triple-negative breast cancer cells. (A) MDA-MB-231 (10 µM; n=3; 24 h) and (B) Hs578T cells (4 µM; n=3; 24 h). Scale bar, 100 µM. CEP, cepharanthine.

    Journal: Molecular Medicine Reports

    Article Title: Cepharanthine inhibits lysosomes and induces apoptosis in triple-negative breast cancer cells

    doi: 10.3892/mmr.2026.13899

    Figure Lengend Snippet: CEP induces ΔΨm loss in triple-negative breast cancer cells. (A) MDA-MB-231 (10 µM; n=3; 24 h) and (B) Hs578T cells (4 µM; n=3; 24 h). Scale bar, 100 µM. CEP, cepharanthine.

    Article Snippet: The TNBC cell lines MDA-MB-231 (cat. no. CL-0150; Procell Life Science & Technology Co., Ltd.) and Hs578T (cat. no. CL-0114; Procell Life Science & Technology Co., Ltd.) were cultured in high-glucose DMEM medium (cat. no. C11965500BT; Gibco; Thermo Fisher Scientific, Inc.), supplemented with 10% fetal bovine serum (cat. no. 164210; Procell Life Science & Technology Co., Ltd.) and 1% penicillin/streptomycin (cat. no. 15140122; Gibco; Thermo Fisher Scientific, Inc.).

    Techniques:

    CEP upregulates NOXA and downregulates Bcl-2 expression in triple-negative breast cancer cells. CEP upregulated NOXA and downregulated Bcl-2 expression in (A) MDA-MB-231 cells (n=3; 24 h) and (B) Hs578T cells (n=3; 24 h). *P<0.05, **P<0.01 and ***P<0.001 vs. control. CEP, cepharanthine; NOXA, phorbol-12-myristate-13-acetate-induced protein 1; ns, not significant.

    Journal: Molecular Medicine Reports

    Article Title: Cepharanthine inhibits lysosomes and induces apoptosis in triple-negative breast cancer cells

    doi: 10.3892/mmr.2026.13899

    Figure Lengend Snippet: CEP upregulates NOXA and downregulates Bcl-2 expression in triple-negative breast cancer cells. CEP upregulated NOXA and downregulated Bcl-2 expression in (A) MDA-MB-231 cells (n=3; 24 h) and (B) Hs578T cells (n=3; 24 h). *P<0.05, **P<0.01 and ***P<0.001 vs. control. CEP, cepharanthine; NOXA, phorbol-12-myristate-13-acetate-induced protein 1; ns, not significant.

    Article Snippet: The TNBC cell lines MDA-MB-231 (cat. no. CL-0150; Procell Life Science & Technology Co., Ltd.) and Hs578T (cat. no. CL-0114; Procell Life Science & Technology Co., Ltd.) were cultured in high-glucose DMEM medium (cat. no. C11965500BT; Gibco; Thermo Fisher Scientific, Inc.), supplemented with 10% fetal bovine serum (cat. no. 164210; Procell Life Science & Technology Co., Ltd.) and 1% penicillin/streptomycin (cat. no. 15140122; Gibco; Thermo Fisher Scientific, Inc.).

    Techniques: Expressing, Control

    CEP treatment activates TFEB in triple-negative breast cancer cells. (A) CEP triggers TFEB nuclear translocation in MDA-MB-231 cells (10 µM; 24 h). (B) CEP triggers TFEB nuclear translocation in Hs578T cells (4 µM; 24 h). Scale bar, 25 µm; n=5. **P<0.01 vs. control. TFEB, transcription factor EB; CEP, cepharanthine.

    Journal: Molecular Medicine Reports

    Article Title: Cepharanthine inhibits lysosomes and induces apoptosis in triple-negative breast cancer cells

    doi: 10.3892/mmr.2026.13899

    Figure Lengend Snippet: CEP treatment activates TFEB in triple-negative breast cancer cells. (A) CEP triggers TFEB nuclear translocation in MDA-MB-231 cells (10 µM; 24 h). (B) CEP triggers TFEB nuclear translocation in Hs578T cells (4 µM; 24 h). Scale bar, 25 µm; n=5. **P<0.01 vs. control. TFEB, transcription factor EB; CEP, cepharanthine.

    Article Snippet: The TNBC cell lines MDA-MB-231 (cat. no. CL-0150; Procell Life Science & Technology Co., Ltd.) and Hs578T (cat. no. CL-0114; Procell Life Science & Technology Co., Ltd.) were cultured in high-glucose DMEM medium (cat. no. C11965500BT; Gibco; Thermo Fisher Scientific, Inc.), supplemented with 10% fetal bovine serum (cat. no. 164210; Procell Life Science & Technology Co., Ltd.) and 1% penicillin/streptomycin (cat. no. 15140122; Gibco; Thermo Fisher Scientific, Inc.).

    Techniques: Translocation Assay, Control

    CEP does not induce LMP, increase lysosomal pH or destabilize lysosomal membrane-bound enzymes in triple-negative breast cancer cells. (A) CEP does not induce LMP, as evidenced by a dispersion of dextran, in MDA-MB-231 (10 µM; 24 h) and Hs578T cells (4 µM; 24 h). Scale bar, 25 µm. (B) CEP does not elevate lysosomal pH in the MDA-MB-231 (10 µM; 24 h) and Hs578T cell lines (4 µM; 24 h). Scale bar, 25 µm. (C) CEP does not induce lysosomal membrane-bound enzymes degradation in the MDA-MB-231 (10 µM; n=3; 24 h) and Hs578T cell lines (4 µM; n=3; 24 h). CEP, cepharanthine; ASAH1, acid ceramidase; SMPD1, sphingomyelin phosphodiesterase; ns, not significant.

    Journal: Molecular Medicine Reports

    Article Title: Cepharanthine inhibits lysosomes and induces apoptosis in triple-negative breast cancer cells

    doi: 10.3892/mmr.2026.13899

    Figure Lengend Snippet: CEP does not induce LMP, increase lysosomal pH or destabilize lysosomal membrane-bound enzymes in triple-negative breast cancer cells. (A) CEP does not induce LMP, as evidenced by a dispersion of dextran, in MDA-MB-231 (10 µM; 24 h) and Hs578T cells (4 µM; 24 h). Scale bar, 25 µm. (B) CEP does not elevate lysosomal pH in the MDA-MB-231 (10 µM; 24 h) and Hs578T cell lines (4 µM; 24 h). Scale bar, 25 µm. (C) CEP does not induce lysosomal membrane-bound enzymes degradation in the MDA-MB-231 (10 µM; n=3; 24 h) and Hs578T cell lines (4 µM; n=3; 24 h). CEP, cepharanthine; ASAH1, acid ceramidase; SMPD1, sphingomyelin phosphodiesterase; ns, not significant.

    Article Snippet: The TNBC cell lines MDA-MB-231 (cat. no. CL-0150; Procell Life Science & Technology Co., Ltd.) and Hs578T (cat. no. CL-0114; Procell Life Science & Technology Co., Ltd.) were cultured in high-glucose DMEM medium (cat. no. C11965500BT; Gibco; Thermo Fisher Scientific, Inc.), supplemented with 10% fetal bovine serum (cat. no. 164210; Procell Life Science & Technology Co., Ltd.) and 1% penicillin/streptomycin (cat. no. 15140122; Gibco; Thermo Fisher Scientific, Inc.).

    Techniques: Membrane, Dispersion

    Cell viability (MTT assay) of HUVEC (A) and HS578T cells (B) treated for 48 h with commercial DTX or the DTX:γ-CD complex. Results expressed as mean ± SD ( n = 6). Statistical analysis by two-way ANOVA plus Tukey–Kramer post hoc: * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001.

    Journal: ACS Omega

    Article Title: Complexation of the Antineoplastic Docetaxel with γ‑Cyclodextrin Significantly Improves Its Solubility and In Vitro Safety

    doi: 10.1021/acsomega.5c10596

    Figure Lengend Snippet: Cell viability (MTT assay) of HUVEC (A) and HS578T cells (B) treated for 48 h with commercial DTX or the DTX:γ-CD complex. Results expressed as mean ± SD ( n = 6). Statistical analysis by two-way ANOVA plus Tukey–Kramer post hoc: * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001.

    Article Snippet: Human umbilical vein endothelial cells (HUVEC, ATCC PCS-100–010) and human breast cancer cells HS578T (ATCC HTB-126) were used in this study.

    Techniques: MTT Assay