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hs21  (ATCC)


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    ATCC hs21
    Bacterial strains used for analysis and validation of the capsule multiplex PCR.
    Hs21, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hs21/product/ATCC
    Average 90 stars, based on 1 article reviews
    hs21 - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Updated Campylobacter jejuni Capsule PCR Multiplex Typing System and Its Application to Clinical Isolates from South and Southeast Asia"

    Article Title: Updated Campylobacter jejuni Capsule PCR Multiplex Typing System and Its Application to Clinical Isolates from South and Southeast Asia

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0144349

    Bacterial strains used for analysis and validation of the capsule multiplex PCR.
    Figure Legend Snippet: Bacterial strains used for analysis and validation of the capsule multiplex PCR.

    Techniques Used: Biomarker Discovery, Multiplex Assay

    Summary of the primer sequences included in the C . jejuni capsule multiplex typing scheme.
    Figure Legend Snippet: Summary of the primer sequences included in the C . jejuni capsule multiplex typing scheme.

    Techniques Used: Multiplex Assay, Sequencing

    Summary of the C . jejuni capsule multiplex PCR expected results.
    Figure Legend Snippet: Summary of the C . jejuni capsule multiplex PCR expected results.

    Techniques Used: Multiplex Assay

    Capsule multiplex PCR results among all C . jejuni isolates from different population group from South and South-East Asia. In bold are the five most prevalent CPS types in their respective group. Number in parentheses represent the number of strain in the category.
    Figure Legend Snippet: Capsule multiplex PCR results among all C . jejuni isolates from different population group from South and South-East Asia. In bold are the five most prevalent CPS types in their respective group. Number in parentheses represent the number of strain in the category.

    Techniques Used: Multiplex Assay



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    Bacterial strains used for analysis and validation of the capsule multiplex PCR.
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    <t>The</t> <t>HS21/45</t> plasmid and the <t>CAR</t> insert were then ligated and subjected to sequencing to verify the correct orientation. ( C ) The vav -CAR plasmid was then prepared using an endotoxin free kit, digested with HindIII and ( D ) the transgene microinjected into C57BL/6 fertilized oocytes. Not to scale.
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    <t>The</t> <t>HS21/45</t> plasmid and the <t>CAR</t> insert were then ligated and subjected to sequencing to verify the correct orientation. ( C ) The vav -CAR plasmid was then prepared using an endotoxin free kit, digested with HindIII and ( D ) the transgene microinjected into C57BL/6 fertilized oocytes. Not to scale.
    Liquid Chromatographer Assembly Of Hs21 1 Uv Detector, Bt 22b Peristaltic Pump, Th2000 Gradient Generator, And 3057 Recorder, supplied by Shanghai QingPu HuXi, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Bacterial strains used for analysis and validation of the capsule multiplex PCR.

    Journal: PLoS ONE

    Article Title: Updated Campylobacter jejuni Capsule PCR Multiplex Typing System and Its Application to Clinical Isolates from South and Southeast Asia

    doi: 10.1371/journal.pone.0144349

    Figure Lengend Snippet: Bacterial strains used for analysis and validation of the capsule multiplex PCR.

    Article Snippet: ATCC 43447 , HS21 , [ ] .

    Techniques: Biomarker Discovery, Multiplex Assay

    Summary of the primer sequences included in the C . jejuni capsule multiplex typing scheme.

    Journal: PLoS ONE

    Article Title: Updated Campylobacter jejuni Capsule PCR Multiplex Typing System and Its Application to Clinical Isolates from South and Southeast Asia

    doi: 10.1371/journal.pone.0144349

    Figure Lengend Snippet: Summary of the primer sequences included in the C . jejuni capsule multiplex typing scheme.

    Article Snippet: ATCC 43447 , HS21 , [ ] .

    Techniques: Multiplex Assay, Sequencing

    Summary of the C . jejuni capsule multiplex PCR expected results.

    Journal: PLoS ONE

    Article Title: Updated Campylobacter jejuni Capsule PCR Multiplex Typing System and Its Application to Clinical Isolates from South and Southeast Asia

    doi: 10.1371/journal.pone.0144349

    Figure Lengend Snippet: Summary of the C . jejuni capsule multiplex PCR expected results.

    Article Snippet: ATCC 43447 , HS21 , [ ] .

    Techniques: Multiplex Assay

    Capsule multiplex PCR results among all C . jejuni isolates from different population group from South and South-East Asia. In bold are the five most prevalent CPS types in their respective group. Number in parentheses represent the number of strain in the category.

    Journal: PLoS ONE

    Article Title: Updated Campylobacter jejuni Capsule PCR Multiplex Typing System and Its Application to Clinical Isolates from South and Southeast Asia

    doi: 10.1371/journal.pone.0144349

    Figure Lengend Snippet: Capsule multiplex PCR results among all C . jejuni isolates from different population group from South and South-East Asia. In bold are the five most prevalent CPS types in their respective group. Number in parentheses represent the number of strain in the category.

    Article Snippet: ATCC 43447 , HS21 , [ ] .

    Techniques: Multiplex Assay

    The HS21/45 plasmid and the CAR insert were then ligated and subjected to sequencing to verify the correct orientation. ( C ) The vav -CAR plasmid was then prepared using an endotoxin free kit, digested with HindIII and ( D ) the transgene microinjected into C57BL/6 fertilized oocytes. Not to scale.

    Journal: PLoS ONE

    Article Title: Expression of a Chimeric Antigen Receptor in Multiple Leukocyte Lineages in Transgenic Mice

    doi: 10.1371/journal.pone.0140543

    Figure Lengend Snippet: The HS21/45 plasmid and the CAR insert were then ligated and subjected to sequencing to verify the correct orientation. ( C ) The vav -CAR plasmid was then prepared using an endotoxin free kit, digested with HindIII and ( D ) the transgene microinjected into C57BL/6 fertilized oocytes. Not to scale.

    Article Snippet: The HS21/45 DNA plasmid containing the CAR insert ( vav -CAR) was prepared using an Endofree kit (Qiagen, Netherlands) to isolate endotoxin-free DNA.

    Techniques: Plasmid Preparation, Sequencing

    Genomic DNA from WT, heterozygous and homozygous vav-CAR mice was amplified using primers specific for the endogenous gene flanking the transgene insertion point (WT primers) or within the transgene itself (CAR primers). Amplified DNA was then analyzed on a 1% agarose gel. (A) F38 mice, lane L 1 kb Plus DNA ladder, lanes 1–2 wild type C57BL/6 mice, lanes 3–6 homozygous mice, lanes 7–10 heterozygous mice. (B) F9 mice, lane L Promega 100 bp ladder, lanes 1–2 wild type C57BL/6 mice, lanes 3–4 heterozygous mice, lanes 5–6 homozygous mice.

    Journal: PLoS ONE

    Article Title: Expression of a Chimeric Antigen Receptor in Multiple Leukocyte Lineages in Transgenic Mice

    doi: 10.1371/journal.pone.0140543

    Figure Lengend Snippet: Genomic DNA from WT, heterozygous and homozygous vav-CAR mice was amplified using primers specific for the endogenous gene flanking the transgene insertion point (WT primers) or within the transgene itself (CAR primers). Amplified DNA was then analyzed on a 1% agarose gel. (A) F38 mice, lane L 1 kb Plus DNA ladder, lanes 1–2 wild type C57BL/6 mice, lanes 3–6 homozygous mice, lanes 7–10 heterozygous mice. (B) F9 mice, lane L Promega 100 bp ladder, lanes 1–2 wild type C57BL/6 mice, lanes 3–4 heterozygous mice, lanes 5–6 homozygous mice.

    Article Snippet: The HS21/45 DNA plasmid containing the CAR insert ( vav -CAR) was prepared using an Endofree kit (Qiagen, Netherlands) to isolate endotoxin-free DNA.

    Techniques: Amplification, Agarose Gel Electrophoresis