a32961 hoechst 33342 medchemexpress  (MedChemExpress)

Journal: Poultry Science

Article Title: Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolin

doi: 10.1016/j.psj.2026.106920

Figure Lengend Snippet: Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) Hoechst 33258 staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.

Article Snippet: 300 μL of Hoechst 33258 dye solution (Beyotime Biotechnology, China) was added to each well to cover the cells, and the cells were stained in an incubator for 20 min (37 °C, 5 % CO 2 ).

Techniques: Staining, Flow Cytometry

Journal: JBMR Plus

Article Title: Crosstalk with bone marrow adipocytes, but not osteoblasts, drives a cortical bone resorption phenotype in female mice with adult-onset deletion of the glucocorticoid receptor in Osterix-expressing cells

doi: 10.1093/jbmrpl/ziag090

Figure Lengend Snippet: Validation of automated cell quantification. (A) BMSCs were isolated from 12-wk-old CD-1 mice ( n = 3 biological replicates per group), seeded, and treated with dexamethasone for 14 d. At day 14, cells were fixed, stained with Oil Red O (ORO) and counterstained with Hoechst. Quantification was performed using a Cytation 5 plate reader and Gen5 software. (B) Linear regression model evaluating the impact of different fluorescence intensities for comparing automated quantification of ORO positive cells vs manual quantification. (C) Manual and automated total cell counts of osteogenic cultures treated with, and without dexamethasone. There were no statistically significant differences between the quantification methods. (D) Manual and automated ratio of ORO positive cells to total cells. Both methods were able to detect glucocorticoid-induced lipid storage. (E and F) GR-CKO BMSC-derived osteoblasts had increased lipid storage compared to osteoblasts derived from WT littermates ( n = 3 biological replicates per genotype and sex). Bar charts show group mean ± SE. Each data point represents one biological replicate. p -values for variables assessed by 2-factor analysis of variance (ANOVA), Fisher’s LSD post-hoc testing, and t -tests are shown.

Article Snippet: To visualize and quantify lipid storage, cells were stained with 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY; MedChemExpress #HY-W090090) or Oil Red O (ORO; Sigma-Aldrich #O1391) to detect lipids and Hoechst (1 μg; Fisher Scientific #H3570) to highlight the nucleus and permit accurate quantification of total cell number.

Techniques: Biomarker Discovery, Isolation, Staining, Software, Fluorescence, Derivative Assay