primary human lecs hmvec-dly  (Lonza)

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Enhancement of Lymphangiogenesis In Vitro via the Regulations of HIF-1 α Expression and Nuclear Translocation by Deoxyshikonin

doi: 10.1155/2013/148297

Figure Lengend Snippet: Effects of deoxyshikonin on cord formation of HMVEC-dLy and HMVEC-d on Matrigel. (a), (c) Photographs of cord formation of HMVEC-dLy and HMVEC-d on Matrigel after incubation with or without 0.8 μ M deoxyshikonin at 2 to 6 h (at ×400 magnification). (b), (d) The relative length of cords was measured using an Angiogenesis Image Analyzer. Data are the mean ± SD ( n = 3); * P < 0.05, ** P < 0.01 compared with the control.

Article Snippet: Human dermal lymphatic microvascular endothelial cells (HMVEC-dLy) and human dermal microvascular endothelial cells (HMVEC-d) were obtained from Takara Bio Inc. (Shiga, Japan).

Techniques: Incubation

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Enhancement of Lymphangiogenesis In Vitro via the Regulations of HIF-1 α Expression and Nuclear Translocation by Deoxyshikonin

doi: 10.1155/2013/148297

Figure Lengend Snippet: Effect of deoxyshikonin on VEGF-C, -A, and -D mRNA levels and regulation of HIF-1 α during cord formation of HMVEC-dLy on Matrigel. Cells were exposed with or without 0.8 μ M deoxyshikonin for 6 h and then underwent experiments. (a) The relative length of cords was measured using an Angiogenesis Image Analyzer. (b), (c) VEGF-C, -A, and -D and also HIF-1 α mRNA levels were detected by real-time PCR. (d) The HIF-1 α protein level was determined by immunoprecipitation and Western blotting. The results were analyzed by scanning and Scion Image software. (e) HIF-1 α nuclear translocation as determined by immunofluorescence microscopy. Similar results were obtained in three independent experiments; * P < 0.05, ** P < 0.01 compared with their control.

Article Snippet: Human dermal lymphatic microvascular endothelial cells (HMVEC-dLy) and human dermal microvascular endothelial cells (HMVEC-d) were obtained from Takara Bio Inc. (Shiga, Japan).

Techniques: Real-time Polymerase Chain Reaction, Immunoprecipitation, Western Blot, Software, Translocation Assay, Immunofluorescence, Microscopy

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Enhancement of Lymphangiogenesis In Vitro via the Regulations of HIF-1 α Expression and Nuclear Translocation by Deoxyshikonin

doi: 10.1155/2013/148297

Figure Lengend Snippet: Deoxyshikonin regulates VEGF-C mRNA levels and cord formation of HMVEC-dLy via HIF-1 α . After transfection with control siRNA or siRNA for HIF-1 α , HMVEC-dLy cells were seeded on Matrigel and incubated with or without 0.8 μ M deoxyshikonin for 2–6 h, and then cells were subjected to real-time PCR and cord formation assay. (a) HIF-1 α mRNA levels. (b) VEGF-C mRNA levels. (c) Relative length of cord formations. Similar results were obtained in three independent experiments; * P < 0.05, ** P < 0.01 compared with the control.

Article Snippet: Human dermal lymphatic microvascular endothelial cells (HMVEC-dLy) and human dermal microvascular endothelial cells (HMVEC-d) were obtained from Takara Bio Inc. (Shiga, Japan).

Techniques: Transfection, Incubation, Real-time Polymerase Chain Reaction, Tube Formation Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Enhancement of Lymphangiogenesis In Vitro via the Regulations of HIF-1 α Expression and Nuclear Translocation by Deoxyshikonin

doi: 10.1155/2013/148297

Figure Lengend Snippet: Deoxyshikonin promoted wound healing in vitro by inducing the HMVEC-dLy migration ability. After growing the HMVEC-dLy in culture inserts that create a cell-free gap, migration of cells to fill the gap was monitored at regular intervals. (a) Photograph of cell migration into the gap from 0 to 24 h in deoxyshikonin treatment and control group. (b) The migration ability of the cells was measured using Leica LAS EZ software and the migration distances calculated (mm). (c) The effects of deoxyshikonin on cell proliferation were determined by a proliferation assay and the data are plotted as percentages of control cell proliferation. Data are the mean ± SD ( n = 3); * P < 0.05, ** P < 0.01 compared with the control.

Article Snippet: Human dermal lymphatic microvascular endothelial cells (HMVEC-dLy) and human dermal microvascular endothelial cells (HMVEC-d) were obtained from Takara Bio Inc. (Shiga, Japan).

Techniques: In Vitro, Migration, Software, Proliferation Assay