hgastrin i (Tocris)
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hgastrin i
Hgastrin I, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hgastrin/Gastrin+I+(human)/bio_rxiv__64898__2026__04__18__719329-262-39-41
Average 96 stars, based on 172 article reviews
Hgastrin I, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hgastrin/Gastrin+I+(human)/bio_rxiv__64898__2026__04__18__719329-262-39-41
Average 96 stars, based on 172 article reviews
hgastrin i - by Bioz Stars,
2026-11
96/100 stars
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Cell Culture:Article Title: Development of Gemcitabine-Modified miRNA Mimics as Cancer Therapeutics for Pancreatic Ductal Adenocarcinoma Article Snippet: .. In brief, organoid cells were cultured with Corning TM Matrigel TM GFR Membrane Matrix (Corning Incorporated, Corning, NY, USA) and incubated at 37°C in Human Complete Feeding Medium (hCPLT) consisting of the following: Advanced DMEM/F12 (Thermo Fisher Scientific, Waltham, MA, USA), HEPES (final concentration, 10 mmol/L) (Thermo Fisher Scientific, Waltham, MA, USA), GlutaMAX (final concentration, 1x) (Thermo Fisher Scientific, Waltham, MA, USA), A 83-01 (final concentration, 500 nmol/L) (Tocris Bioscience, Bristol, UK), hEGF (final concentration, 50 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), mNoggin (final concentration, 100 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), hFGF10 (final concentration, 100 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), Article Title: Development of gemcitabine-modified miRNA mimics as cancer therapeutics for pancreatic ductal adenocarcinoma Article Snippet: .. In brief, organoid cells were cultured with Corning Matrigel GFR Membrane Matrix (Corning Incorporated, Corning, NY, USA) and incubated at 37°C in Human Complete Feeding Medium (hCPLT) consisting of the following: Advanced DMEM/F12 (Thermo Fisher Scientific, Waltham, MA, USA), HEPES (final concentration, 10 mmol/L) (Thermo Fisher Scientific, Waltham, MA, USA), GlutaMAX (final concentration, 1x) (Thermo Fisher Scientific, Waltham, MA, USA), A 83-01 (final concentration, 500 nmol/L) (Tocris Bioscience, Bristol, UK), hEGF (final concentration, 50 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), mNoggin (final concentration, 100 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), hFGF10 (final concentration, 100 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), Article Title: KRAS G 12C -inhibitor-based combination therapies for pancreatic cancer: insights from drug screening. Article Snippet: .. For propagation, PDOs were cultured in cultivation media consisting of Advanced DMEM/F-12 medium (#12634028; Gibco) supplemented with 10 mM HEPES (#15630080; Gibco), 19-GlutaMAX (#35050061; Gibco), and 0.1% BSA (#A9576; SigmaAldrich), 10% R-spondin1-Conditioned medium (Rspondin1-Conditioned medium overexpressing cell line HEK293T), 19-B27 (#17504001; Thermo-Fischer), 10 nM Nicotinamide (#N0636; Sigma-Aldrich), 1.25 mM N-acetylcysteine (#A9165; Sigma-Aldrich), 100 lg mL 1 Primocin (#ant-pm-2; Invivogen), 100 ng mL 1 mNoggin (#250-38; Peprotech), 100 ng mL 1 hFGF10 (#100- 26; Peprotech), 10 nM Membrane:Article Title: Development of Gemcitabine-Modified miRNA Mimics as Cancer Therapeutics for Pancreatic Ductal Adenocarcinoma Article Snippet: .. In brief, organoid cells were cultured with Corning TM Matrigel TM GFR Membrane Matrix (Corning Incorporated, Corning, NY, USA) and incubated at 37°C in Human Complete Feeding Medium (hCPLT) consisting of the following: Advanced DMEM/F12 (Thermo Fisher Scientific, Waltham, MA, USA), HEPES (final concentration, 10 mmol/L) (Thermo Fisher Scientific, Waltham, MA, USA), GlutaMAX (final concentration, 1x) (Thermo Fisher Scientific, Waltham, MA, USA), A 83-01 (final concentration, 500 nmol/L) (Tocris Bioscience, Bristol, UK), hEGF (final concentration, 50 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), mNoggin (final concentration, 100 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), hFGF10 (final concentration, 100 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), Article Title: Development of gemcitabine-modified miRNA mimics as cancer therapeutics for pancreatic ductal adenocarcinoma Article Snippet: .. In brief, organoid cells were cultured with Corning Matrigel GFR Membrane Matrix (Corning Incorporated, Corning, NY, USA) and incubated at 37°C in Human Complete Feeding Medium (hCPLT) consisting of the following: Advanced DMEM/F12 (Thermo Fisher Scientific, Waltham, MA, USA), HEPES (final concentration, 10 mmol/L) (Thermo Fisher Scientific, Waltham, MA, USA), GlutaMAX (final concentration, 1x) (Thermo Fisher Scientific, Waltham, MA, USA), A 83-01 (final concentration, 500 nmol/L) (Tocris Bioscience, Bristol, UK), hEGF (final concentration, 50 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), mNoggin (final concentration, 100 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), hFGF10 (final concentration, 100 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), Incubation:Article Title: Development of Gemcitabine-Modified miRNA Mimics as Cancer Therapeutics for Pancreatic Ductal Adenocarcinoma Article Snippet: .. In brief, organoid cells were cultured with Corning TM Matrigel TM GFR Membrane Matrix (Corning Incorporated, Corning, NY, USA) and incubated at 37°C in Human Complete Feeding Medium (hCPLT) consisting of the following: Advanced DMEM/F12 (Thermo Fisher Scientific, Waltham, MA, USA), HEPES (final concentration, 10 mmol/L) (Thermo Fisher Scientific, Waltham, MA, USA), GlutaMAX (final concentration, 1x) (Thermo Fisher Scientific, Waltham, MA, USA), A 83-01 (final concentration, 500 nmol/L) (Tocris Bioscience, Bristol, UK), hEGF (final concentration, 50 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), mNoggin (final concentration, 100 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), hFGF10 (final concentration, 100 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), Article Title: Development of gemcitabine-modified miRNA mimics as cancer therapeutics for pancreatic ductal adenocarcinoma Article Snippet: .. In brief, organoid cells were cultured with Corning Matrigel GFR Membrane Matrix (Corning Incorporated, Corning, NY, USA) and incubated at 37°C in Human Complete Feeding Medium (hCPLT) consisting of the following: Advanced DMEM/F12 (Thermo Fisher Scientific, Waltham, MA, USA), HEPES (final concentration, 10 mmol/L) (Thermo Fisher Scientific, Waltham, MA, USA), GlutaMAX (final concentration, 1x) (Thermo Fisher Scientific, Waltham, MA, USA), A 83-01 (final concentration, 500 nmol/L) (Tocris Bioscience, Bristol, UK), hEGF (final concentration, 50 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), mNoggin (final concentration, 100 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), hFGF10 (final concentration, 100 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), Concentration Assay:Article Title: Development of Gemcitabine-Modified miRNA Mimics as Cancer Therapeutics for Pancreatic Ductal Adenocarcinoma Article Snippet: .. In brief, organoid cells were cultured with Corning TM Matrigel TM GFR Membrane Matrix (Corning Incorporated, Corning, NY, USA) and incubated at 37°C in Human Complete Feeding Medium (hCPLT) consisting of the following: Advanced DMEM/F12 (Thermo Fisher Scientific, Waltham, MA, USA), HEPES (final concentration, 10 mmol/L) (Thermo Fisher Scientific, Waltham, MA, USA), GlutaMAX (final concentration, 1x) (Thermo Fisher Scientific, Waltham, MA, USA), A 83-01 (final concentration, 500 nmol/L) (Tocris Bioscience, Bristol, UK), hEGF (final concentration, 50 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), mNoggin (final concentration, 100 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), hFGF10 (final concentration, 100 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), Article Title: Development of gemcitabine-modified miRNA mimics as cancer therapeutics for pancreatic ductal adenocarcinoma Article Snippet: .. In brief, organoid cells were cultured with Corning Matrigel GFR Membrane Matrix (Corning Incorporated, Corning, NY, USA) and incubated at 37°C in Human Complete Feeding Medium (hCPLT) consisting of the following: Advanced DMEM/F12 (Thermo Fisher Scientific, Waltham, MA, USA), HEPES (final concentration, 10 mmol/L) (Thermo Fisher Scientific, Waltham, MA, USA), GlutaMAX (final concentration, 1x) (Thermo Fisher Scientific, Waltham, MA, USA), A 83-01 (final concentration, 500 nmol/L) (Tocris Bioscience, Bristol, UK), hEGF (final concentration, 50 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), mNoggin (final concentration, 100 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), hFGF10 (final concentration, 100 ng/mL) (Thermo Fisher Scientific, Waltham, MA, USA), other:Article Title: TP63-Mediated Enhancer Reprogramming Drives the Squamous Subtype of Pancreatic Ductal Adenocarcinoma Article Snippet: Organoids:Article Title: Preclinical Screening of Splice-Switching Antisense Oligonucleotides in PDAC Organoids Article Snippet: For organoids dissociation, cells were treated with TrypLE Express (Thermo Fisher) and then resuspend with Matrigel (Corning). .. Human Complete Feeding Medium (hCPLT) (H+++B minus BSA, Wnt3a-conditioned medium [1x, cell line from ATCC], R-spondin1-conditioned medium [1x, cell line from Trevigen], B27 supplement [1x, Thermo Fisher], Nicotinamide [10 mM, Sigma-Aldrich], N-acetylcysteine [1.25 mM, Sigma-Aldrich], mNoggin [100 ng/mL, Peprotech], hEGF [50 ng/mL, Peprotech], hFGF [100 ng/mL, Peprotech], |