human hair follicle dermal papilla cells  (PromoCell)

Journal: Bioengineering

Article Title: A Novel Approach to Pattern Dermal Papilla Spheroids in Dermal–Epidermal Composites Using Non-Adherent Microwell Arrays

doi: 10.3390/bioengineering12121281

Figure Lengend Snippet: Representative human dermal papilla cell spheroids formed using the hanging drop method. Top row: Here, 0.1 mg/mL Corning Rat Tail Collagen I was added to a 10 µL suspension of 3000 HDPCs and cultured for 24 h. Bottom row: No collagen was added to the cells. Scale bar = 100 µm.

Article Snippet: Human dermal papilla cells (HDPCs) (PromoCell, Heidelberg, Germany) were cultured in complete Follicle Dermal Papilla Cell Growth Medium (HDP-M) (PromoCell, Heidelberg, Germany) supplemented with 1% Penicillin–Streptomycin (Gibco, Grand Island, NY, USA), 0.1% Gentamicin (Gibco), and 0.2% Amphotericin (Gibco).

Techniques: Suspension, Cell Culture

Journal: Bioengineering

Article Title: A Novel Approach to Pattern Dermal Papilla Spheroids in Dermal–Epidermal Composites Using Non-Adherent Microwell Arrays

doi: 10.3390/bioengineering12121281

Figure Lengend Snippet: Singularized HDPC response to culture in egg crate-designed microwell arrays fabricated from different non-adherent substrate materials. Stereolithography 3D printed stamps with an egg crate microwell pattern were used to mold 2% agarose ( A ), 5% agarose ( B ), and PDMS ( C ) substrate materials. HDPCs at 3000 cells per microwell were added to the arrays, cultured for 24 h, and imaged using light microscopy. The softer 2% and 5% agarose hydrogel substrate materials did not result in spheroid formation but did effectively pattern the singularized HDPCs. The stiffer silicone elastomer PDMS microwell arrays did result in HDPC spheroid formation and patterning when seeded with singularized HDPCs. Scale bars = 200 µm.

Article Snippet: Human dermal papilla cells (HDPCs) (PromoCell, Heidelberg, Germany) were cultured in complete Follicle Dermal Papilla Cell Growth Medium (HDP-M) (PromoCell, Heidelberg, Germany) supplemented with 1% Penicillin–Streptomycin (Gibco, Grand Island, NY, USA), 0.1% Gentamicin (Gibco), and 0.2% Amphotericin (Gibco).

Techniques: Cell Culture, Light Microscopy

Journal: Bioengineering

Article Title: A Novel Approach to Pattern Dermal Papilla Spheroids in Dermal–Epidermal Composites Using Non-Adherent Microwell Arrays

doi: 10.3390/bioengineering12121281

Figure Lengend Snippet: Distance between HDPC spheroids over time. Quantification of the distance of patterned HDPC spheroids formed in PDMS microwell arrays (1 d), transferred to the collagen matrix dermal compartment (3 d), cultured in epidermalization media (5 d), and transitioned to the air–liquid interface. The average distance of patterned spheroids decreases as the collagen matrix contracts and is reorganized by HDPCs. Scale bars = 200 µm. Note that the 1 d image on the top left is the same image shown in C. Significance comparing microwell spheroid distance at 1 day to later timepoints is denoted at statistical levels: *** p < 0.001.

Article Snippet: Human dermal papilla cells (HDPCs) (PromoCell, Heidelberg, Germany) were cultured in complete Follicle Dermal Papilla Cell Growth Medium (HDP-M) (PromoCell, Heidelberg, Germany) supplemented with 1% Penicillin–Streptomycin (Gibco, Grand Island, NY, USA), 0.1% Gentamicin (Gibco), and 0.2% Amphotericin (Gibco).

Techniques: Cell Culture

Journal: bioRxiv

Article Title: Basement membrane components define the microenvironment of aggregated fibroblasts in the skin and support their aggregation in vitro

doi: 10.1101/2025.08.27.672757

Figure Lengend Snippet: (A) Schematic of the spheroid invasion assay. DP spheroids, each composed of 300 HFDPCs, were placed on an ECM gel layer and overlaid with an additional ECM gel layer. The spheroids were cultured and observed every 12 hours. (B) Phase-contrast images of DP spheroids cultured in collagen I gel (top) and Matrigel (bottom). The colored squares indicate regions magnified in the bottom right images. Scale bar: 100 µm. (C) Binary-masked images highlighting the spheroid area. (D) Line graphs showing the changes in spheroid area over time in collagen I gel (red) and Matrigel (green). (E) Box plots showing the relative spheroid area at 12 h, normalized to the initial time point (1 h). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Two-tailed unpaired t -tests were used: * p <0.05, ** p <0.01, and *** p <0.001. n = 9 wells. (F and G) Box plots showing the relative spheroid area with integrin blocking antibodies in collagen I gel (F) and Matrigel (G). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Statistical comparisons were performed using the Kruskal‒Wallis test followed by Dunn’s post hoc test. Adjusted p -values from the Benjamini‒Hochberg method are shown: * p <0.05, ** p <0.01, *** p <0.001. n = 9 wells pooled from 3 independent experiments.

Article Snippet: Primary human follicle DP cells (HFDPCs) were purchased from PromoCell (Heidelberg, Germany) and cultured in Follicle Papillae Growth Medium (PromoCell) at 37°C, 5% CO 2 and 100% humidity.

Techniques: Invasion Assay, Cell Culture, Two Tailed Test, Blocking Assay