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hek-blue detection  (InvivoGen)


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    InvivoGen hek-blue detection
    Hek Blue Detection, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 2781 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hek/HEK-Blue+Detection/custom%40hb-det3%4042571814
    Average 97 stars, based on 2781 article reviews
    hek-blue detection - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Design, synthesis and biological evaluation of novel pyrimidine and quinazoline-based small-molecule Toll-like receptor 8 antagonists.
    Article Snippet: .. Human Embryonic Kidney (HEK)-Blue Null1, HEK-Blue hTLR2TLR1, HEK-Blue hTLR2-TLR6, HEK-Blue hTLR4, HEK-Blue hTLR5, HEKBlue hTLR7, HEK-Blue hTLR8, and HEK-Blue hTLR9 cells (InvivoGen, Toulouse, France) were cultured in Dulbecco's modified Eagle's medium (PAN-Biotech, Aidenbach, Germany) containing 10% (v/v) heat inactivated fetal bovine serum (FBS; S0615), 100 U/mL penicillin, 100 mg/mL streptomycin (P4333), 2 mM L-glutamine (G7513) (all from Sigma Aldrich, Taufkirchen, Germany), 100 μg/mL normocin and selective antibiotics HEK-Blue Selection (hTLR2-TLR1, hTLR2-TLR6, hTLR4), 100 μg/mL zeocin (Null1) and 100 μg/mL zeocin with 10 μg/mL (hTLR7, hTLR9) resp. 30 μg/mL (hTLR5, hTLR8) blasticidin (all from InvivoGen, Toulouse, France). .. THP1-Dual TLR4/MD-2/CD14 cells (InvivoGen, Toulouse, France) were cultured in RPMI-1640 medium supplemented with 10% FBS, penicillin (100U/mL), streptomycin (100 μg/mL), L-glutamine (2 mM), HEPES (25 mM), normocin (100 μg/ mL) and selective antibiotics (blasticidin: 10 μg/mL, zeocin: 100 μg/mL) following the manufacturer's instructions.

    Modification:

    Article Title: Design, synthesis and biological evaluation of novel pyrimidine and quinazoline-based small-molecule Toll-like receptor 8 antagonists.
    Article Snippet: .. Human Embryonic Kidney (HEK)-Blue Null1, HEK-Blue hTLR2TLR1, HEK-Blue hTLR2-TLR6, HEK-Blue hTLR4, HEK-Blue hTLR5, HEKBlue hTLR7, HEK-Blue hTLR8, and HEK-Blue hTLR9 cells (InvivoGen, Toulouse, France) were cultured in Dulbecco's modified Eagle's medium (PAN-Biotech, Aidenbach, Germany) containing 10% (v/v) heat inactivated fetal bovine serum (FBS; S0615), 100 U/mL penicillin, 100 mg/mL streptomycin (P4333), 2 mM L-glutamine (G7513) (all from Sigma Aldrich, Taufkirchen, Germany), 100 μg/mL normocin and selective antibiotics HEK-Blue Selection (hTLR2-TLR1, hTLR2-TLR6, hTLR4), 100 μg/mL zeocin (Null1) and 100 μg/mL zeocin with 10 μg/mL (hTLR7, hTLR9) resp. 30 μg/mL (hTLR5, hTLR8) blasticidin (all from InvivoGen, Toulouse, France). .. THP1-Dual TLR4/MD-2/CD14 cells (InvivoGen, Toulouse, France) were cultured in RPMI-1640 medium supplemented with 10% FBS, penicillin (100U/mL), streptomycin (100 μg/mL), L-glutamine (2 mM), HEPES (25 mM), normocin (100 μg/ mL) and selective antibiotics (blasticidin: 10 μg/mL, zeocin: 100 μg/mL) following the manufacturer's instructions.

    Article Title: Mannose derivatives of ferrocene-containing desmuramyl peptides: Synthesis, characterization and in vitro immunological evaluation.
    Article Snippet: Muramyl dipeptide is a bona fide nucleotide-binding oligomerization-domain-containing protein 2 (NOD2) agonist with well-established adjuvant properties.. Replacement of its N-acetylmuramyl moiety and installation of lipophilic aromatic fragments have been utilized to afford desmuramyl peptides with improved pharmacological properties.. Herein, we have constructed mannosylated desmuramyl peptides conjugated with ferrocene via amide or ester bonds incorporating linkers of different lengths.

    Selection:

    Article Title: Design, synthesis and biological evaluation of novel pyrimidine and quinazoline-based small-molecule Toll-like receptor 8 antagonists.
    Article Snippet: .. Human Embryonic Kidney (HEK)-Blue Null1, HEK-Blue hTLR2TLR1, HEK-Blue hTLR2-TLR6, HEK-Blue hTLR4, HEK-Blue hTLR5, HEKBlue hTLR7, HEK-Blue hTLR8, and HEK-Blue hTLR9 cells (InvivoGen, Toulouse, France) were cultured in Dulbecco's modified Eagle's medium (PAN-Biotech, Aidenbach, Germany) containing 10% (v/v) heat inactivated fetal bovine serum (FBS; S0615), 100 U/mL penicillin, 100 mg/mL streptomycin (P4333), 2 mM L-glutamine (G7513) (all from Sigma Aldrich, Taufkirchen, Germany), 100 μg/mL normocin and selective antibiotics HEK-Blue Selection (hTLR2-TLR1, hTLR2-TLR6, hTLR4), 100 μg/mL zeocin (Null1) and 100 μg/mL zeocin with 10 μg/mL (hTLR7, hTLR9) resp. 30 μg/mL (hTLR5, hTLR8) blasticidin (all from InvivoGen, Toulouse, France). .. THP1-Dual TLR4/MD-2/CD14 cells (InvivoGen, Toulouse, France) were cultured in RPMI-1640 medium supplemented with 10% FBS, penicillin (100U/mL), streptomycin (100 μg/mL), L-glutamine (2 mM), HEPES (25 mM), normocin (100 μg/ mL) and selective antibiotics (blasticidin: 10 μg/mL, zeocin: 100 μg/mL) following the manufacturer's instructions.

    Article Title: From Sea to Cell: Ascophyllum nodosum and Fucus vesiculosus Extracts Attenuate NF-κB-Mediated Inflammation and Protect Intestinal Barrier Integrity—A Comprehensive Analysis Applying In Vitro and In Vivo Models
    Article Snippet: .. The culture medium was supplemented with 10% heat-inactivated FBS, penicillin–streptomycin (100 U/mL-100 μg/mL), 100 μg/mL NormocinTM (InvivoGen), and the appropriate selection antibiotics: 1× HEK-Blue Selection for HEK-Blue hTLR4 cells or 100 μg/mL Zeocin for HEK-Blue null2 cells. ..

    Produced:

    Article Title: Utilizing vaccines to treat cancer and enhance the success rate of cancer immunotherapy
    Article Snippet: Toll-Like Receptor (TLR) Signaling Assay The HEK-Blue-mTLR7 cell line (Invivogen), engineered to express mouse TLR7 (mTLR7), and its parental cell line, Null2-k (Invivogen), which does not express any mouse TLRs, were propagated and utilized to determine TLR7 activity, as per the manufacturer's instructions. .. In HEK-Blue-mTLR7 cells, secreted embryonic alkaline phosphatase (SEAP) is produced upon stimulation with a TLR7 ligand and real-time detection of SEAP is conducted utilizing HEK-Blue Detection medium (Invivogen) (7). .. Briefly, treatments and controls were plated in triplicate: 20 μl of active (MOI=1) or heat-inactivated influenza A/PR8/1934/H1N1 virus, positive control TLR7 agonist, CL264 (50 μg/ml, Invivogen), and negative control, PBS (1×).

    Adjuvant:

    Article Title: A novel multi-epitope vaccine induces protective and therapeutic immunity against Helicobacter pylori
    Article Snippet: .. To evaluate the adjuvant function of flagellin arms in the MEU protein in vitro, the HEK-Blue-Luci hTLR5 cells (InvivoGen, France) were employed, expressing the human TLR5 receptor and an inducible secretory embryonic alkaline phosphatase (SEAP) under an NF-κB-inducible promoter. ..

    In Vitro:

    Article Title: A novel multi-epitope vaccine induces protective and therapeutic immunity against Helicobacter pylori
    Article Snippet: .. To evaluate the adjuvant function of flagellin arms in the MEU protein in vitro, the HEK-Blue-Luci hTLR5 cells (InvivoGen, France) were employed, expressing the human TLR5 receptor and an inducible secretory embryonic alkaline phosphatase (SEAP) under an NF-κB-inducible promoter. ..

    Expressing:

    Article Title: A novel multi-epitope vaccine induces protective and therapeutic immunity against Helicobacter pylori
    Article Snippet: .. To evaluate the adjuvant function of flagellin arms in the MEU protein in vitro, the HEK-Blue-Luci hTLR5 cells (InvivoGen, France) were employed, expressing the human TLR5 receptor and an inducible secretory embryonic alkaline phosphatase (SEAP) under an NF-κB-inducible promoter. ..



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    Image Search Results


    MiR-136-3p content in cell culture medium and uptake of extracellular miR-136-3p into cultured myotubes. MiRNA content in (A) human myotubes and (B) human pancreatic islets culture media. Results were first normalized using RNU1A1 and then presented in relation to miR-23a-3p content for n = 4 different donors for myotubes cultures and n = 4 donors for human islets. Left panel (C) shows bright-field image of cultured human myotubes and right panel (C) shows a representative fluorescence image of cultured human myotubes with cells exposed to human serum-derived EVs loaded with Cy3-miR-136-3p. Cy3 fluorescence (red) is detected in the whole cytoplasm of the human myotubes. (D) Representative fluorescence image of human myotubes exposed to HEK293 culture medium with EVs loaded with Cy3-miR-136-3p (red). (E) Representative image of human myotubes exposed to EVs loaded with TexasRed-labeled with a control RNA (orange). Nuclear Hoechst staining is shown in blue. Scale bar = 100 μm. EVs = extracellular vesicles; HEK293 = human embryonic kidney; miR = microRNA; Rel = relative; RNU1A1 = U1 small nuclear RNA.

    Journal: Journal of Sport and Health Science

    Article Title: Exercise training-induced extracellular miR-136-3p modulates glucose uptake and myogenesis through targeting of NRDC in human skeletal muscle

    doi: 10.1016/j.jshs.2025.101091

    Figure Lengend Snippet: MiR-136-3p content in cell culture medium and uptake of extracellular miR-136-3p into cultured myotubes. MiRNA content in (A) human myotubes and (B) human pancreatic islets culture media. Results were first normalized using RNU1A1 and then presented in relation to miR-23a-3p content for n = 4 different donors for myotubes cultures and n = 4 donors for human islets. Left panel (C) shows bright-field image of cultured human myotubes and right panel (C) shows a representative fluorescence image of cultured human myotubes with cells exposed to human serum-derived EVs loaded with Cy3-miR-136-3p. Cy3 fluorescence (red) is detected in the whole cytoplasm of the human myotubes. (D) Representative fluorescence image of human myotubes exposed to HEK293 culture medium with EVs loaded with Cy3-miR-136-3p (red). (E) Representative image of human myotubes exposed to EVs loaded with TexasRed-labeled with a control RNA (orange). Nuclear Hoechst staining is shown in blue. Scale bar = 100 μm. EVs = extracellular vesicles; HEK293 = human embryonic kidney; miR = microRNA; Rel = relative; RNU1A1 = U1 small nuclear RNA.

    Article Snippet: Human embryonic kidney (HEK293) cells were obtained from American Type Culture Collection (ATCC) and cultured in high-glucose (4.5 g/L) Dulbecco's Modified Eagle Medium (DMEM; Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 10% (vol/vol) FBS.

    Techniques: Cell Culture, Fluorescence, Derivative Assay, Labeling, Control, Staining

    NRDC is a direct target of miR-136-3p in human myotubes. Skeletal muscle NRDC mRNA is responsive to training and inactivity. (A) Tissue mRNA expression of NRDC from the Human Protein Atlas database showing enriched expression of NRDC in human skeletal muscle. (B) The miR-136-3p target site in the NRDC gene is highly conserved in mammals. (C) Luciferase activity in HEK293 cells co-transfected the NRDC 3’UTR and miR-136-3p with or without anti-miR136-3p inhibitors. miR-136-3p transfection downregulates NRDC (D) mRNA and (E) representative image of protein abundance in human myotubes. (F) Publicly available data ( GSE14413 ) showing NRDC mRNA expression in human skeletal muscle of healthy young participants after 6 weeks of endurance training ( n = 8). (G) Publicly available data ( GSE120862 ) showing NRDC mRNA expression in human skeletal muscle of healthy young participants after 2 months of aerobic training ( n = 10). (H) Publicly available data ( GSE14901 ) showing NRDC mRNA expression in human skeletal muscle of healthy young participants after 14 days of immobilization ( n = 24). * p < 0.05, ** p < 0.005. GSE = gene set enrichment; HEK293 = human embryonic kidney; miR = microRNA; NC = negative control; NRDC = nardilysin convertase; nTPM = normalized transcripts per million; si NRDC = small interfering RNA of NRDC ; UTR = untranslated region.

    Journal: Journal of Sport and Health Science

    Article Title: Exercise training-induced extracellular miR-136-3p modulates glucose uptake and myogenesis through targeting of NRDC in human skeletal muscle

    doi: 10.1016/j.jshs.2025.101091

    Figure Lengend Snippet: NRDC is a direct target of miR-136-3p in human myotubes. Skeletal muscle NRDC mRNA is responsive to training and inactivity. (A) Tissue mRNA expression of NRDC from the Human Protein Atlas database showing enriched expression of NRDC in human skeletal muscle. (B) The miR-136-3p target site in the NRDC gene is highly conserved in mammals. (C) Luciferase activity in HEK293 cells co-transfected the NRDC 3’UTR and miR-136-3p with or without anti-miR136-3p inhibitors. miR-136-3p transfection downregulates NRDC (D) mRNA and (E) representative image of protein abundance in human myotubes. (F) Publicly available data ( GSE14413 ) showing NRDC mRNA expression in human skeletal muscle of healthy young participants after 6 weeks of endurance training ( n = 8). (G) Publicly available data ( GSE120862 ) showing NRDC mRNA expression in human skeletal muscle of healthy young participants after 2 months of aerobic training ( n = 10). (H) Publicly available data ( GSE14901 ) showing NRDC mRNA expression in human skeletal muscle of healthy young participants after 14 days of immobilization ( n = 24). * p < 0.05, ** p < 0.005. GSE = gene set enrichment; HEK293 = human embryonic kidney; miR = microRNA; NC = negative control; NRDC = nardilysin convertase; nTPM = normalized transcripts per million; si NRDC = small interfering RNA of NRDC ; UTR = untranslated region.

    Article Snippet: Human embryonic kidney (HEK293) cells were obtained from American Type Culture Collection (ATCC) and cultured in high-glucose (4.5 g/L) Dulbecco's Modified Eagle Medium (DMEM; Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 10% (vol/vol) FBS.

    Techniques: Expressing, Luciferase, Activity Assay, Transfection, Quantitative Proteomics, Negative Control, Small Interfering RNA