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Structured Review

Proteintech membranes
Membranes, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hdac6/HDAC6+Antibody/10__1016_slash_j__lddd__2026__100552-102-8-24
Average 95 stars, based on 97 article reviews
membranes - by Bioz Stars, 2026-11
95/100 stars

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Related Articles

Incubation:

Article Title: Targeting ADAMTS1/HDAC6 alleviates TGF-β1/SMAD2-associated cardiac fibrosis in cardiac fibrosis post-myocardial infarction.
Article Snippet: .. Primary antibodies against VIM (10366-1-AP, 1:200, Proteintech), HDAC6 (12834-1-AP, 1:50, Proteintech), ADAMTS1 (12749-1-AP, 1:50, Proteintech), FN (AWA10327, 1:50, Abiowell), Collagen I (ab138492, 1:200, Abcam), and p-SMAD2 (ab280888, 1:50, Abcam) were incubated at 4 °C overnight. .. Goat anti-mouse IgG (H+L) (AWS0003a, Abiowell) or goat anti-rabbit IgG (H+L) (AWS0006a/AWS0005a, Abiowell) (50–100 μL) was incubated for 90 min at 37 °C.

Article Title: Targeting ADAMTS1/HDAC6 alleviates TGF-β1/SMAD2-associated cardiac fibrosis in cardiac fibrosis post-myocardial infarction
Article Snippet: .. Membrane was initially probed with primary antibodies against ADAMTS1 (ab236497, 1:2000, Abcam) and HDAC6 (128,341-AP, 1:1000, Proteintech), followed by incubation with matching secondary antibodies. ..

Article Title: Targeting ADAMTS1/HDAC6 alleviates TGF-β1/SMAD2-associated cardiac fibrosis in cardiac fibrosis post-myocardial infarction
Article Snippet: .. The blocked membranes were incubated overnight at 4 °C with the following primary antibodies: ADAMTS1 (ab236497, 1:2000, Abcam), Collagen I (ab270993, 1:1000, Abcam), FN ( AWA00143 , 1:1000, Abiowell), SMAD2 (12,570–1-AP, 1:5000, Proteintech), p-SMAD2 (ab280888, 1:1000, Abcam), α-SMA (ab5694, 1 μg/ml, Abcam), HDAC6 (128,341-AP, 1:1000, Proteintech), TGF-β1 ( AWA00083 , 1:1000, Abiowell), and GAPDH (10,494–1-AP, 1:5000, Proteintech) at 4 °C overnight. .. The membrane was then incubated with HRP-conjugated goat anti-mouse/rabbit IgG (SA00001-1/SA00001-2, 1:5000/1:6000, Proteintech) for 1.5 h. Protein bands were visualized by incubating membranes with enhanced ECL reagent (AWB0005, Abiowell) for 1 min, followed by imaging with a chemiluminescence imaging system.

Article Title: Targeting ADAMTS1/HDAC6 alleviates TGF-β1/SMAD2-associated cardiac fibrosis in cardiac fibrosis post-myocardial infarction.
Article Snippet: .. Membrane was initially probed with primary antibodies against ADAMTS1 (ab236497, 1:2000, Abcam) and HDAC6 (128341-AP, 1:1000, Proteintech), followed by incubation with matching secondary antibodies. ..

Membrane:

Article Title: Targeting ADAMTS1/HDAC6 alleviates TGF-β1/SMAD2-associated cardiac fibrosis in cardiac fibrosis post-myocardial infarction
Article Snippet: .. Membrane was initially probed with primary antibodies against ADAMTS1 (ab236497, 1:2000, Abcam) and HDAC6 (128,341-AP, 1:1000, Proteintech), followed by incubation with matching secondary antibodies. ..

Article Title: Targeting ADAMTS1/HDAC6 alleviates TGF-β1/SMAD2-associated cardiac fibrosis in cardiac fibrosis post-myocardial infarction.
Article Snippet: .. Membrane was initially probed with primary antibodies against ADAMTS1 (ab236497, 1:2000, Abcam) and HDAC6 (128341-AP, 1:1000, Proteintech), followed by incubation with matching secondary antibodies. ..

Cell-Signaling:

Article Title: THBS1 upregulation by KLF7 in hypopharyngeal squamous cell carcinoma contributes to lung metastasis through the p38 MAPK signaling
Article Snippet: After incubation with 5% BSA at room temperature for 2 h, the membranes were maintained with the primary antibody as indicated at 4°C overnight and then incubated with the secondary antibody at room temperature for 1 h. Protein bands were visualized with a High Sensitivity ECL Kit (HY-K2005, MedChemExpress). .. The primary antibodies used were as follows: anti-THBS1 (1:250, A2125, BioAb), anti-E-cadherin (1:1000, 3195, Cell Signaling Technologies), anti-N-cadherin (1:100, PA5-19486m Invitrogen), anti-Phospho-p38 MAPK (Thr180/Tyr182) (1:1000, 4511, Cell Signaling Technologies), anti-total p38 (1:1000, 9212, Cell Signaling Technologies), anti- p -ATF2 (Thr71) (1:1000, 44-295G, Invitrogen), anti-p-MK2 (Thr334) (1:1000, 3007, Cell Signaling Technologies), anti-KLF7 (1:1000, CSB- PA126129 , Cusabio), anti-Slug (1:1000, 12129-1-AP, ProteinTech Group), anti-Vimentin (1:20000, 10366-1-AP, ProteinTech Group), anti-HDAC6 (1:1000, 12834-1-AP, ProteinTech Group), and anti-GAPDH (1:20000, AC001, BioAb). .. ChIP assays were performed using the SimpleChIP Enzymatic ChIP Kit (9003, Cell Signaling Technologies).



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