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hct 116 atcc  (ATCC)


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    Structured Review

    ATCC hct 116 atcc
    Hct 116 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 17830 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hct/HCT+116/pmc13224021-38-11-12
    Average 99 stars, based on 17830 article reviews
    hct 116 atcc - by Bioz Stars, 2026-08
    99/100 stars

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    Validation of CL16-RhoA binding by LC-MS/MS experiments (A) Workflow of LC-MS/MS experiments to detect RhoA-CL16 engagement in <t>HCT116</t> cells. CL16-treated cells were harvested, digested and analyzed by LC-MS/MS. (B) Representative MS/MS showing CL16 modification on RhoA Cys16 in HCT116 cells. (C) Workflow of LC-MS/MS experiments using CL16-alkyne (a CL16-molecular probe) to study target profile of CL16. (D) Volcano plot revealing protein targets of CL16 identified by CL16-molecular probe. Statistical analyses were performed by two-tailed Student’s t-test by MS Excel. (E) Venn diagram summarizing the protein targets of CL16 identified in (A) and (C), highlighting RhoA as the primary target.
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    Hirschmann hct 9 grid partitions
    Hirschmann‐CPAK <t>(HCT‐9)</t> reading grid. CPAK, Coronal Plane Alignment of the Knee; FMA, femoral mechanical angle; HCT‐9, Hirschmann–CPAK Translational grid; TMA, tibial mechanical angle.
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    Validation of CL16-RhoA binding by LC-MS/MS experiments (A) Workflow of LC-MS/MS experiments to detect RhoA-CL16 engagement in HCT116 cells. CL16-treated cells were harvested, digested and analyzed by LC-MS/MS. (B) Representative MS/MS showing CL16 modification on RhoA Cys16 in HCT116 cells. (C) Workflow of LC-MS/MS experiments using CL16-alkyne (a CL16-molecular probe) to study target profile of CL16. (D) Volcano plot revealing protein targets of CL16 identified by CL16-molecular probe. Statistical analyses were performed by two-tailed Student’s t-test by MS Excel. (E) Venn diagram summarizing the protein targets of CL16 identified in (A) and (C), highlighting RhoA as the primary target.

    Journal: STAR Protocols

    Article Title: Protocol to identify covalent inhibitors targeting RhoA Cys16

    doi: 10.1016/j.xpro.2026.104494

    Figure Lengend Snippet: Validation of CL16-RhoA binding by LC-MS/MS experiments (A) Workflow of LC-MS/MS experiments to detect RhoA-CL16 engagement in HCT116 cells. CL16-treated cells were harvested, digested and analyzed by LC-MS/MS. (B) Representative MS/MS showing CL16 modification on RhoA Cys16 in HCT116 cells. (C) Workflow of LC-MS/MS experiments using CL16-alkyne (a CL16-molecular probe) to study target profile of CL16. (D) Volcano plot revealing protein targets of CL16 identified by CL16-molecular probe. Statistical analyses were performed by two-tailed Student’s t-test by MS Excel. (E) Venn diagram summarizing the protein targets of CL16 identified in (A) and (C), highlighting RhoA as the primary target.

    Article Snippet: Human: HCT116 (Wildtype/48Y/Male) , ATCC , #CCL-247.

    Techniques: Biomarker Discovery, Binding Assay, Liquid Chromatography with Mass Spectroscopy, Tandem Mass Spectroscopy, Modification, Two Tailed Test

    Hirschmann‐CPAK (HCT‐9) reading grid. CPAK, Coronal Plane Alignment of the Knee; FMA, femoral mechanical angle; HCT‐9, Hirschmann–CPAK Translational grid; TMA, tibial mechanical angle.

    Journal: Journal of Experimental Orthopaedics

    Article Title: Speaking the same language? A direct cartography between functional knee phenotypes and CPAK

    doi: 10.1002/jeo2.70801

    Figure Lengend Snippet: Hirschmann‐CPAK (HCT‐9) reading grid. CPAK, Coronal Plane Alignment of the Knee; FMA, femoral mechanical angle; HCT‐9, Hirschmann–CPAK Translational grid; TMA, tibial mechanical angle.

    Article Snippet: The HCT‐9 grid partitions the space of Hirschmann functional phenotypes (FMA, TMA) into a 3 × 3 matrix ranging from A1 to C3 according to CPAK‐derived thresholds applied to the anatomical axes.

    Techniques: