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hcmecs  (PromoCell)


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    Structured Review

    PromoCell hcmecs
    Hcmecs, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hcmecs/Human+Cardiac+Microvascular+Endothelial+Cells/pmc13170363-103-0-2
    Average 95 stars, based on 144 article reviews
    hcmecs - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria
    Article Snippet: Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria.
    Article Snippet: Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Endothelial RRM2B-dependent mitochondrial DNA signalling drives doxorubicin-cardiotoxicity
    Article Snippet: Human endothelial cell lines HUVECs (pooled donors, C2519A, Lonza) and HCMECs (Caucasian male, C-12285 PromoCell) were used between passage 3 and 6.. Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery.
    Article Snippet: Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier. .. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2. .. The cells were cultured at 37 °C in a humidified chamber with 5% CO2.

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery
    Article Snippet: The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2.. The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .

    In Vitro:

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria
    Article Snippet: Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria.
    Article Snippet: Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Endothelial RRM2B-dependent mitochondrial DNA signalling drives doxorubicin-cardiotoxicity
    Article Snippet: Human endothelial cell lines HUVECs (pooled donors, C2519A, Lonza) and HCMECs (Caucasian male, C-12285 PromoCell) were used between passage 3 and 6.. Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery.
    Article Snippet: Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier. .. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2. .. The cells were cultured at 37 °C in a humidified chamber with 5% CO2.

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery
    Article Snippet: The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2.. The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .

    Western Blot:

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria
    Article Snippet: Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria.
    Article Snippet: Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Endothelial RRM2B-dependent mitochondrial DNA signalling drives doxorubicin-cardiotoxicity
    Article Snippet: Human endothelial cell lines HUVECs (pooled donors, C2519A, Lonza) and HCMECs (Caucasian male, C-12285 PromoCell) were used between passage 3 and 6.. Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery.
    Article Snippet: Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier. .. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2. .. The cells were cultured at 37 °C in a humidified chamber with 5% CO2.

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery
    Article Snippet: The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2.. The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .

    Comparison:

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria
    Article Snippet: Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria.
    Article Snippet: Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Endothelial RRM2B-dependent mitochondrial DNA signalling drives doxorubicin-cardiotoxicity
    Article Snippet: Human endothelial cell lines HUVECs (pooled donors, C2519A, Lonza) and HCMECs (Caucasian male, C-12285 PromoCell) were used between passage 3 and 6.. Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery.
    Article Snippet: Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier. .. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2. .. The cells were cultured at 37 °C in a humidified chamber with 5% CO2.

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery
    Article Snippet: The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2.. The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .

    Expressing:

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria
    Article Snippet: Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria.
    Article Snippet: Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Endothelial RRM2B-dependent mitochondrial DNA signalling drives doxorubicin-cardiotoxicity
    Article Snippet: Human endothelial cell lines HUVECs (pooled donors, C2519A, Lonza) and HCMECs (Caucasian male, C-12285 PromoCell) were used between passage 3 and 6.. Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery.
    Article Snippet: Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier. .. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2. .. The cells were cultured at 37 °C in a humidified chamber with 5% CO2.

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery
    Article Snippet: The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2.. The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .

    Quantitative RT-PCR:

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria
    Article Snippet: Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria.
    Article Snippet: Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Endothelial RRM2B-dependent mitochondrial DNA signalling drives doxorubicin-cardiotoxicity
    Article Snippet: Human endothelial cell lines HUVECs (pooled donors, C2519A, Lonza) and HCMECs (Caucasian male, C-12285 PromoCell) were used between passage 3 and 6.. Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery.
    Article Snippet: Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier. .. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2. .. The cells were cultured at 37 °C in a humidified chamber with 5% CO2.

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery
    Article Snippet: The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2.. The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .

    Immunostaining:

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria
    Article Snippet: Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria.
    Article Snippet: Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Endothelial RRM2B-dependent mitochondrial DNA signalling drives doxorubicin-cardiotoxicity
    Article Snippet: Human endothelial cell lines HUVECs (pooled donors, C2519A, Lonza) and HCMECs (Caucasian male, C-12285 PromoCell) were used between passage 3 and 6.. Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery.
    Article Snippet: Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier. .. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2. .. The cells were cultured at 37 °C in a humidified chamber with 5% CO2.

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery
    Article Snippet: The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2.. The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .

    MANN-WHITNEY:

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria
    Article Snippet: Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria.
    Article Snippet: Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Endothelial RRM2B-dependent mitochondrial DNA signalling drives doxorubicin-cardiotoxicity
    Article Snippet: Human endothelial cell lines HUVECs (pooled donors, C2519A, Lonza) and HCMECs (Caucasian male, C-12285 PromoCell) were used between passage 3 and 6.. Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery.
    Article Snippet: Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier. .. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2. .. The cells were cultured at 37 °C in a humidified chamber with 5% CO2.

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery
    Article Snippet: The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2.. The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .

    Control:

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria
    Article Snippet: Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria.
    Article Snippet: Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Endothelial RRM2B-dependent mitochondrial DNA signalling drives doxorubicin-cardiotoxicity
    Article Snippet: Human endothelial cell lines HUVECs (pooled donors, C2519A, Lonza) and HCMECs (Caucasian male, C-12285 PromoCell) were used between passage 3 and 6.. Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery.
    Article Snippet: Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier. .. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2. .. The cells were cultured at 37 °C in a humidified chamber with 5% CO2.

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery
    Article Snippet: The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2.. The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .

    Cell Culture:

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria
    Article Snippet: Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria.
    Article Snippet: Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Endothelial RRM2B-dependent mitochondrial DNA signalling drives doxorubicin-cardiotoxicity
    Article Snippet: Human endothelial cell lines HUVECs (pooled donors, C2519A, Lonza) and HCMECs (Caucasian male, C-12285 PromoCell) were used between passage 3 and 6.. Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery.
    Article Snippet: Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier. .. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2. .. The cells were cultured at 37 °C in a humidified chamber with 5% CO2.

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery
    Article Snippet: The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2.. The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .

    Transfection:

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria
    Article Snippet: Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria.
    Article Snippet: Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Endothelial RRM2B-dependent mitochondrial DNA signalling drives doxorubicin-cardiotoxicity
    Article Snippet: Human endothelial cell lines HUVECs (pooled donors, C2519A, Lonza) and HCMECs (Caucasian male, C-12285 PromoCell) were used between passage 3 and 6.. Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery.
    Article Snippet: Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier. .. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2. .. The cells were cultured at 37 °C in a humidified chamber with 5% CO2.

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery
    Article Snippet: The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2.. The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .

    Negative Control:

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria
    Article Snippet: Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria.
    Article Snippet: Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Endothelial RRM2B-dependent mitochondrial DNA signalling drives doxorubicin-cardiotoxicity
    Article Snippet: Human endothelial cell lines HUVECs (pooled donors, C2519A, Lonza) and HCMECs (Caucasian male, C-12285 PromoCell) were used between passage 3 and 6.. Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery.
    Article Snippet: Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier. .. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2. .. The cells were cultured at 37 °C in a humidified chamber with 5% CO2.

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery
    Article Snippet: The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2.. The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .

    Proliferation Assay:

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria
    Article Snippet: Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria.
    Article Snippet: Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Endothelial RRM2B-dependent mitochondrial DNA signalling drives doxorubicin-cardiotoxicity
    Article Snippet: Human endothelial cell lines HUVECs (pooled donors, C2519A, Lonza) and HCMECs (Caucasian male, C-12285 PromoCell) were used between passage 3 and 6.. Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery.
    Article Snippet: Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier. .. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2. .. The cells were cultured at 37 °C in a humidified chamber with 5% CO2.

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery
    Article Snippet: The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2.. The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .

    Enzyme-linked Immunosorbent Assay:

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria
    Article Snippet: Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria.
    Article Snippet: Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Endothelial RRM2B-dependent mitochondrial DNA signalling drives doxorubicin-cardiotoxicity
    Article Snippet: Human endothelial cell lines HUVECs (pooled donors, C2519A, Lonza) and HCMECs (Caucasian male, C-12285 PromoCell) were used between passage 3 and 6.. Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery.
    Article Snippet: Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier. .. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2. .. The cells were cultured at 37 °C in a humidified chamber with 5% CO2.

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery
    Article Snippet: The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2.. The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .

    Membrane:

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria
    Article Snippet: Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experiments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria.
    Article Snippet: Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.Experi ments were performed in serum-free endothelial cell media supplemented only with 1% PS.. HCMECs from PromoCell (C-12285) were used up to passage 8.. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061).

    Article Title: Endothelial RRM2B-dependent mitochondrial DNA signalling drives doxorubicin-cardiotoxicity
    Article Snippet: Human endothelial cell lines HUVECs (pooled donors, C2519A, Lonza) and HCMECs (Caucasian male, C-12285 PromoCell) were used between passage 3 and 6.. Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .Cells were cultured onto 0.2% −0.5% gelatin-coated plates with Endothelial Cell Growth Medium 2 Kit and Endothelial Cell Growth Medium MV 2 Kit supplemented with 2% and 5% foetal calf serum at 37 °C in a humidified atmosphere and 5% CO 2 and 5% O 2 .

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery.
    Article Snippet: Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier. .. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2. .. The cells were cultured at 37 °C in a humidified chamber with 5% CO2.

    Article Title: Transcriptional changes of the extracellular matrix in chronic thromboembolic pulmonary hypertension govern right ventricle remodeling and recovery
    Article Snippet: The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.The HCFs were purchased from ScienCell Research Laboratories (catalog number 6300) and cultured on poly-L-lysine-coated dishes in fibroblast medium as recommended by the supplier.. HCMECs were purchased from PromoCell (catalog number C-12285) and maintained in Endothelial Cell Basal Medium MV2, supplemented with SupplementPack Endothelial Cell GM MV2.. The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .The cells were cultured at 37 °C in a humidified chamber with 5% CO 2 .



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    (A) Schematic of a primary cilium, depicting its core compartments (basal body, transition zone, axoneme, ciliary tip) and the localization of key ciliary proteins ARL13B and RPGRIP1L. Intraflagellar transport (IFT) complexes (IFT-A and IFT-B) facilitate cargo trafficking along the axoneme. Adapted from Smith et al. (2020). (B, C) Immunofluorescence images confirming primary cilia (ARL13B, red) in human cerebral microvascular endothelial cells <t>(hCMEC/D3)</t> (B) and mouse bEnd.3 cells (C) . Nuclei are counterstained with DAPI (blue). Scale bars, 50 µm. (D) Primary cilia (ARL13B, red) in a monolayer of human umbilical vein endothelial cells (HUVECs). Nuclei are stained with DAPI (blue). Scale bar, 50 µm. (E) Primary cilia (ARL13B, red) on endothelial tubules (white arrowheads) and fibroblasts (yellow arrowheads) in a HUVEC–human dermal fibroblast (HDF) organotypic coculture. L, lumen. Nuclei are stained with DAPI (blue). Scale bar: 15 µm. (F, G) Endothelial primary cilia in E13.5 mouse embryonic brain vessels. Cilia (arrows, ARL13B, red) are visible on endothelial cells lining the lumen of a superficial vessel (F) and on intraparenchymal vessels (G) . Endomucin (green (F), brown (G)) marks endothelial cells; nuclei are stained with DAPI (blue). Scale bars, 50 µm (F) and 10 µm (G) .
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    Image Search Results


    (A) Schematic of a primary cilium, depicting its core compartments (basal body, transition zone, axoneme, ciliary tip) and the localization of key ciliary proteins ARL13B and RPGRIP1L. Intraflagellar transport (IFT) complexes (IFT-A and IFT-B) facilitate cargo trafficking along the axoneme. Adapted from Smith et al. (2020). (B, C) Immunofluorescence images confirming primary cilia (ARL13B, red) in human cerebral microvascular endothelial cells (hCMEC/D3) (B) and mouse bEnd.3 cells (C) . Nuclei are counterstained with DAPI (blue). Scale bars, 50 µm. (D) Primary cilia (ARL13B, red) in a monolayer of human umbilical vein endothelial cells (HUVECs). Nuclei are stained with DAPI (blue). Scale bar, 50 µm. (E) Primary cilia (ARL13B, red) on endothelial tubules (white arrowheads) and fibroblasts (yellow arrowheads) in a HUVEC–human dermal fibroblast (HDF) organotypic coculture. L, lumen. Nuclei are stained with DAPI (blue). Scale bar: 15 µm. (F, G) Endothelial primary cilia in E13.5 mouse embryonic brain vessels. Cilia (arrows, ARL13B, red) are visible on endothelial cells lining the lumen of a superficial vessel (F) and on intraparenchymal vessels (G) . Endomucin (green (F), brown (G)) marks endothelial cells; nuclei are stained with DAPI (blue). Scale bars, 50 µm (F) and 10 µm (G) .

    Journal: bioRxiv

    Article Title: Microfluidic analysis reveals ROCK2 regulation of endothelial cilia is essential for blood vessel lumen formation and vascular integrity

    doi: 10.64898/2026.05.27.728336

    Figure Lengend Snippet: (A) Schematic of a primary cilium, depicting its core compartments (basal body, transition zone, axoneme, ciliary tip) and the localization of key ciliary proteins ARL13B and RPGRIP1L. Intraflagellar transport (IFT) complexes (IFT-A and IFT-B) facilitate cargo trafficking along the axoneme. Adapted from Smith et al. (2020). (B, C) Immunofluorescence images confirming primary cilia (ARL13B, red) in human cerebral microvascular endothelial cells (hCMEC/D3) (B) and mouse bEnd.3 cells (C) . Nuclei are counterstained with DAPI (blue). Scale bars, 50 µm. (D) Primary cilia (ARL13B, red) in a monolayer of human umbilical vein endothelial cells (HUVECs). Nuclei are stained with DAPI (blue). Scale bar, 50 µm. (E) Primary cilia (ARL13B, red) on endothelial tubules (white arrowheads) and fibroblasts (yellow arrowheads) in a HUVEC–human dermal fibroblast (HDF) organotypic coculture. L, lumen. Nuclei are stained with DAPI (blue). Scale bar: 15 µm. (F, G) Endothelial primary cilia in E13.5 mouse embryonic brain vessels. Cilia (arrows, ARL13B, red) are visible on endothelial cells lining the lumen of a superficial vessel (F) and on intraparenchymal vessels (G) . Endomucin (green (F), brown (G)) marks endothelial cells; nuclei are stained with DAPI (blue). Scale bars, 50 µm (F) and 10 µm (G) .

    Article Snippet: The following cell lines were cultured in a humidified atmosphere at 37°C with 5% CO 2 . hCMEC/D3 (human cerebral microvascular endothelial cells) (ATCC® CRL-3245TM) were cultured in EndoGRO-MV Complete Media (Merck, SCME004) supplemented with 1 ng/ml basic Fibroblast Growth Factor (bFGF; PeproTech, 100-18B) and used between passages 30-35 for ciliogenesis assays. bEnd.3 (mouse brain endothelial cells) (ATCC® CRL-2299TM) were cultured in DMEM (Gibco, 11995065) supplemented with 10% (v/v) heat-inactivated foetal bovine serum (FBS) (Merck, F9665), 100 U/ml penicillin, 100 μg/ml streptomycin, and 2 mM L-glutamine (all from Gibco). hTERT RPE-1 (human retinal pigment epithelial cells) (ATCC® CRL-4000TM) were cultured in DMEM/F12 (Gibco, 11330032) supplemented with 10% FBS, penicillin/streptomycin, and L-glutamine.

    Techniques: Immunofluorescence, Staining

    RS218 infection induces cell death and tight junction disruption in vitro . (A) Relative LDH release in RS218-infected hCMEC/D3 cells pretreated with calcium chelator BAPTA-AM (10 μM), necroptosis inhibitor Necrostatin-1 (10 μM), caspase-1 inhibitor VX-765 (10 μM), or caspase-3 inhibitor Z-DEVD-FMK (20 μM). (B) LDH release in BV2 cells and murine peritoneal macrophages (PMs) infected with RS218 at MOI = 0.1, 1, 10, and 100 for 1 h and 2 h. (C) Immunofluorescence staining of tight junction protein ZO-1 in hCMEC/D3 cells following RS218 infection. Representative images show DAPI (blue), ZO-1 (green), and merged channels in control and infected cells. Scale bars, 50 μm. Bar graphs show ZO-1 positive area and fluorescence intensity in infected cells relative to controls ( n = 3).

    Journal: Virulence

    Article Title: Integrated transcriptomic and proteomic analysis reveals inflammatory activation and blood-brain barrier disruption during meningitis-associated extraintestinal pathogenic Escherichia coli infection

    doi: 10.1080/21505594.2026.2670939

    Figure Lengend Snippet: RS218 infection induces cell death and tight junction disruption in vitro . (A) Relative LDH release in RS218-infected hCMEC/D3 cells pretreated with calcium chelator BAPTA-AM (10 μM), necroptosis inhibitor Necrostatin-1 (10 μM), caspase-1 inhibitor VX-765 (10 μM), or caspase-3 inhibitor Z-DEVD-FMK (20 μM). (B) LDH release in BV2 cells and murine peritoneal macrophages (PMs) infected with RS218 at MOI = 0.1, 1, 10, and 100 for 1 h and 2 h. (C) Immunofluorescence staining of tight junction protein ZO-1 in hCMEC/D3 cells following RS218 infection. Representative images show DAPI (blue), ZO-1 (green), and merged channels in control and infected cells. Scale bars, 50 μm. Bar graphs show ZO-1 positive area and fluorescence intensity in infected cells relative to controls ( n = 3).

    Article Snippet: For inhibitor experiments, hCMEC/D3 cells were pretreated for 1 h with BAPTA-AM (10 μM), Necrostatin-1 (10 μM), VX-765 (10 μM), or Z-DEVD-FMK (20 μM) (all from MedChemExpress, USA) prior to RS218 infection.

    Techniques: Infection, Disruption, In Vitro, Immunofluorescence, Staining, Control, Fluorescence