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hbmscs  (PromoCell)


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    PromoCell hbmscs
    Hbmscs, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbmscs/product/PromoCell
    Average 96 stars, based on 196 article reviews
    hbmscs - by Bioz Stars, 2026-02
    96/100 stars

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    Interaction between DLX6-AS1 and miR-141-3p. A Predicted target sites. B Dual-luciferase reporter validation of the interaction. C Correlation analysis of serum DLX6-AS1 and miR-141-3p levels. D , E Expression of chondrogenic differentiation markers D , DLX6-AS1, and miR-141-3p E during hBMSCs chondrogenic differentiation. Data are presented as mean ± SD. Statistical analysis was performed using Student’s t-test or one-way ANOVA (n = 3). hBMSCs, Human bone marrow mesenchymal stem cells. * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: Serum DLX6-AS1 and miR-141-3p as early diagnostic biomarkers and regulators of delayed fracture healing (DFH)

    doi: 10.1186/s13018-025-06478-5

    Figure Lengend Snippet: Interaction between DLX6-AS1 and miR-141-3p. A Predicted target sites. B Dual-luciferase reporter validation of the interaction. C Correlation analysis of serum DLX6-AS1 and miR-141-3p levels. D , E Expression of chondrogenic differentiation markers D , DLX6-AS1, and miR-141-3p E during hBMSCs chondrogenic differentiation. Data are presented as mean ± SD. Statistical analysis was performed using Student’s t-test or one-way ANOVA (n = 3). hBMSCs, Human bone marrow mesenchymal stem cells. * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: Human bone marrow mesenchymal stem cells (hBMSCs) were obtained from ATCC (USA).

    Techniques: Luciferase, Biomarker Discovery, Expressing

    Effect of DLX6-AS1/miR-141-3p on hBMSCs functions. A qRT-PCR analysis of DLX6-AS1 and miR-141-3p after si-DLX6-AS1 treatment with or without miR-inhibition. B Proliferation level. C Apoptosis rate. D Expression of chondrogenic differentiation markers SOX9, COL2A1, and ACAN. Data are presented as mean ± SD. Statistical analysis was performed using Student’s t-test or one-way ANOVA (n = 3). hBMSCs, Human bone marrow mesenchymal stem cells. * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: Serum DLX6-AS1 and miR-141-3p as early diagnostic biomarkers and regulators of delayed fracture healing (DFH)

    doi: 10.1186/s13018-025-06478-5

    Figure Lengend Snippet: Effect of DLX6-AS1/miR-141-3p on hBMSCs functions. A qRT-PCR analysis of DLX6-AS1 and miR-141-3p after si-DLX6-AS1 treatment with or without miR-inhibition. B Proliferation level. C Apoptosis rate. D Expression of chondrogenic differentiation markers SOX9, COL2A1, and ACAN. Data are presented as mean ± SD. Statistical analysis was performed using Student’s t-test or one-way ANOVA (n = 3). hBMSCs, Human bone marrow mesenchymal stem cells. * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: Human bone marrow mesenchymal stem cells (hBMSCs) were obtained from ATCC (USA).

    Techniques: Quantitative RT-PCR, Inhibition, Expressing

    VEGFA is a binding target of miR-141-3p. A Predicted binding sequence. B Dual-luciferase assay validation. C VEGFA expression levels in NFH (n = 96) vs DFH (n = 96) patients. D , E Correlation analyses between VEGFA and miR-141-3p or DLX6-AS1 (n = 96). Data are presented as mean ± SD. Statistical analysis was performed using Student’s t-test or one-way ANOVA. hBMSCs, Human bone marrow mesenchymal stem cells. NFH, normal fracture healing; DFH, delayed fracture healing. ** P < 0.01, *** P < 0.001

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: Serum DLX6-AS1 and miR-141-3p as early diagnostic biomarkers and regulators of delayed fracture healing (DFH)

    doi: 10.1186/s13018-025-06478-5

    Figure Lengend Snippet: VEGFA is a binding target of miR-141-3p. A Predicted binding sequence. B Dual-luciferase assay validation. C VEGFA expression levels in NFH (n = 96) vs DFH (n = 96) patients. D , E Correlation analyses between VEGFA and miR-141-3p or DLX6-AS1 (n = 96). Data are presented as mean ± SD. Statistical analysis was performed using Student’s t-test or one-way ANOVA. hBMSCs, Human bone marrow mesenchymal stem cells. NFH, normal fracture healing; DFH, delayed fracture healing. ** P < 0.01, *** P < 0.001

    Article Snippet: Human bone marrow mesenchymal stem cells (hBMSCs) were obtained from ATCC (USA).

    Techniques: Binding Assay, Sequencing, Luciferase, Biomarker Discovery, Expressing

    Functional role of the miR-141-3p/VEGFA axis in hBMSCs. A miR-141-3p and VEGFA abundance after transfection. B Proliferation assessed. C Apoptosis analysis. D Expression of chondrogenic differentiation markers after indicated treatments. Data are presented as mean ± SD. Statistical analysis was performed using Student’s t-test or one-way ANOVA (n = 3). hBMSCs, Human bone marrow mesenchymal stem cells. * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: Serum DLX6-AS1 and miR-141-3p as early diagnostic biomarkers and regulators of delayed fracture healing (DFH)

    doi: 10.1186/s13018-025-06478-5

    Figure Lengend Snippet: Functional role of the miR-141-3p/VEGFA axis in hBMSCs. A miR-141-3p and VEGFA abundance after transfection. B Proliferation assessed. C Apoptosis analysis. D Expression of chondrogenic differentiation markers after indicated treatments. Data are presented as mean ± SD. Statistical analysis was performed using Student’s t-test or one-way ANOVA (n = 3). hBMSCs, Human bone marrow mesenchymal stem cells. * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: Human bone marrow mesenchymal stem cells (hBMSCs) were obtained from ATCC (USA).

    Techniques: Functional Assay, Transfection, Expressing

    A , Schematic illustrating the approach to tuning alginate hydrogel mechanical properties. B , Measurements of hydrogel elastic modulus one day after crosslinking. C , D , Quantifications of stress relaxation half-times across hydrogel matrices. E , Representative images of alkaline phosphatase staining (left) indicating osteogenic differentiation of hBMSCs. Quantification of ALP-positive cells across hydrogel conditions (right). F , Gene expression data for early osteogenic markers from cells cultured across hydrogel conditions. G , Representative micrographs of cells cultured across hydrogel conditions stained for paxillin, β1 integrin, and nuclei (left) with quantifications of both paxillin intensity and colocalization of paxillin with β1 integrin (right). Statistical significance was determined by one-way analysis of variance (ANOVA) followed by Dunnett’s multiple testing correction. n = 3 replicates per condition. * indicates p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: bioRxiv

    Article Title: Matrix stiffness and stress relaxation regulate osteogenesis through histone demethylases KDM4B and KDM6B

    doi: 10.1101/2025.07.16.665229

    Figure Lengend Snippet: A , Schematic illustrating the approach to tuning alginate hydrogel mechanical properties. B , Measurements of hydrogel elastic modulus one day after crosslinking. C , D , Quantifications of stress relaxation half-times across hydrogel matrices. E , Representative images of alkaline phosphatase staining (left) indicating osteogenic differentiation of hBMSCs. Quantification of ALP-positive cells across hydrogel conditions (right). F , Gene expression data for early osteogenic markers from cells cultured across hydrogel conditions. G , Representative micrographs of cells cultured across hydrogel conditions stained for paxillin, β1 integrin, and nuclei (left) with quantifications of both paxillin intensity and colocalization of paxillin with β1 integrin (right). Statistical significance was determined by one-way analysis of variance (ANOVA) followed by Dunnett’s multiple testing correction. n = 3 replicates per condition. * indicates p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: Primary human BMSCs (hBMSCs) were obtained from Millipore Sigma (SCC034) and cultured according to established protocols ( Huebsch et al ., 2010 ; Darnell et al ., 2017 ).

    Techniques: Staining, Gene Expression, Cell Culture