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Proteintech glut4
Glut4, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 226 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glut4/GLUT4+Antibody/pm42915814-21-4-8
Average 96 stars, based on 226 article reviews
glut4 - by Bioz Stars, 2026-11
96/100 stars

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Related Articles

Incubation:

Article Title: MFG-E8-derived antioxidant peptides attenuate dexamethasone-induced skeletal muscle metabolic dysfunction via PI3K/Akt-GLUT4-PPARα signaling pathway.
Article Snippet: Oxidative stress and mitochondrial dysfunction are key features of sarcopenia-related skeletal muscle metabolic dysfunction.. This study aimed to identify MFG-E8-derived bioactive peptides with antioxidant and metabolic regulatory potential.. Integrated bioinformatics analysis highlighted oxidative phosphorylation, thermogenesis, ROS-related pathways and lipid metabolic regulation as major molecular features of sarcopenia-related metabolic dysfunction.

Article Title: Oral Rhizoma Coptis Alkaloids Nanoparticle for Treating Diabetes Through Regulating PI3K/Akt Pathways
Article Snippet: Protein concentration was determined using the BCA protein detection kit (Beyotime, Beijing, China). .. Total protein was separated by SDS-PAGE electrophoresis, transferred to a PVDF membrane blocked with 5% fat-free milk at 4 °C for 60 min, and incubated overnight with primary antibodies IL-1β (1:1000, abcam), IL-6 (1:1000, abcam), TNF-α (1:1000, abcam), IRS1 (1:1000, abcam), p-IRS1 (1:1000, Thermo Fisher Scientific), PI3K (1:2000, proteintech), p-PI3K (1:1000, Thermo Fisher Scientific), AKT (1:5000, proteintech), p-AKT (1:5000, proteintech), GLUT4 (1:2000, proteintech), and β-actin (1/10,000, proteintech). .. Matching horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000, proteintech) were used to evaluate protein expression.

Article Title: Oral Rhizoma Coptis Alkaloids Nanoparticle for Treating Diabetes Through Regulating PI3K/Akt Pathways.
Article Snippet: Protein concentration was determined using the BCA protein detection kit (Beyotime, Beijing, China). .. Total protein was separated by SDS-PAGE electrophoresis, transferred to a PVDF membrane blocked with 5% fat-free milk at 4 ◦C for 60 min, and incubated overnight with primary antibodies IL-1β (1:1000, abcam), IL-6 (1:1000, abcam), TNF-α (1:1000, abcam), IRS1 (1:1000, abcam), p-IRS1 (1:1000, Thermo https://doi.org/10.3390/pharmaceutics18030349 Fisher Scientific), PI3K (1:2000, proteintech), p-PI3K (1:1000, Thermo Fisher Scientific), AKT (1:5000, proteintech), p-AKT (1:5000, proteintech), GLUT4 (1:2000, proteintech), and β-actin (1/10,000, proteintech). .. Matching horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000, proteintech) were used to evaluate protein expression.

Article Title: T-2 toxin induces glycometabolic reprogramming in chondrocytes via a MALAT1/p38‑mediated ceRNA regulatory axis.
Article Snippet: T-2 toxin is a widely distributed dietary mycotoxin and a key environmental driver of Kashin-Beck disease (KBD), yet the molecular events linking exposure to cartilage injury remain incompletely defined.. We examined the chondrotoxic mechanism of T-2 toxin through a MALAT1/miR-15b-5p/MAP2K3 competing endogenous RNA (ceRNA) axis across cellular, animal and human levels.. Rat models of graded T-2 toxin exposure, combined with mRNA and miRNA sequencing identified affected pathways and candidate non-coding RNAs.

Western Blot:

Article Title: cTnIR193H restrictive cardiomyopathy mice satisfy high-energy metabolic demands through regulating glucose metabolism
Article Snippet: Proteins were extracted from mouse heart tissue using RIPA Lysis Buffer (Beyotime, P0013B, China). .. Western blotting analysis was used to detect the expression levels of GLUT4, CD36, PI3K, Akt, and p-Akt using specific antibodies (GLUT4, 1:2000, Proteintech, 66846-1-Ig, China; CD36, 1:500, Wanleibio, WL02390, China; PI3K, 1:1000, Wanleibio, WL03380, China; Akt, 1:500, Wanleibio, WL0003b, China; p-Akt, 1:500, Wanleibio, WLP001a, China). .. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000, ORIGENE, F004, United States) was used as an internal reference.

Analysis:

Article Title: cTnIR193H restrictive cardiomyopathy mice satisfy high-energy metabolic demands through regulating glucose metabolism
Article Snippet: Proteins were extracted from mouse heart tissue using RIPA Lysis Buffer (Beyotime, P0013B, China). .. Western blotting analysis was used to detect the expression levels of GLUT4, CD36, PI3K, Akt, and p-Akt using specific antibodies (GLUT4, 1:2000, Proteintech, 66846-1-Ig, China; CD36, 1:500, Wanleibio, WL02390, China; PI3K, 1:1000, Wanleibio, WL03380, China; Akt, 1:500, Wanleibio, WL0003b, China; p-Akt, 1:500, Wanleibio, WLP001a, China). .. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000, ORIGENE, F004, United States) was used as an internal reference.

Expressing:

Article Title: cTnIR193H restrictive cardiomyopathy mice satisfy high-energy metabolic demands through regulating glucose metabolism
Article Snippet: Proteins were extracted from mouse heart tissue using RIPA Lysis Buffer (Beyotime, P0013B, China). .. Western blotting analysis was used to detect the expression levels of GLUT4, CD36, PI3K, Akt, and p-Akt using specific antibodies (GLUT4, 1:2000, Proteintech, 66846-1-Ig, China; CD36, 1:500, Wanleibio, WL02390, China; PI3K, 1:1000, Wanleibio, WL03380, China; Akt, 1:500, Wanleibio, WL0003b, China; p-Akt, 1:500, Wanleibio, WLP001a, China). .. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000, ORIGENE, F004, United States) was used as an internal reference.

SDS Page:

Article Title: Oral Rhizoma Coptis Alkaloids Nanoparticle for Treating Diabetes Through Regulating PI3K/Akt Pathways
Article Snippet: Protein concentration was determined using the BCA protein detection kit (Beyotime, Beijing, China). .. Total protein was separated by SDS-PAGE electrophoresis, transferred to a PVDF membrane blocked with 5% fat-free milk at 4 °C for 60 min, and incubated overnight with primary antibodies IL-1β (1:1000, abcam), IL-6 (1:1000, abcam), TNF-α (1:1000, abcam), IRS1 (1:1000, abcam), p-IRS1 (1:1000, Thermo Fisher Scientific), PI3K (1:2000, proteintech), p-PI3K (1:1000, Thermo Fisher Scientific), AKT (1:5000, proteintech), p-AKT (1:5000, proteintech), GLUT4 (1:2000, proteintech), and β-actin (1/10,000, proteintech). .. Matching horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000, proteintech) were used to evaluate protein expression.

Article Title: Oral Rhizoma Coptis Alkaloids Nanoparticle for Treating Diabetes Through Regulating PI3K/Akt Pathways.
Article Snippet: Protein concentration was determined using the BCA protein detection kit (Beyotime, Beijing, China). .. Total protein was separated by SDS-PAGE electrophoresis, transferred to a PVDF membrane blocked with 5% fat-free milk at 4 ◦C for 60 min, and incubated overnight with primary antibodies IL-1β (1:1000, abcam), IL-6 (1:1000, abcam), TNF-α (1:1000, abcam), IRS1 (1:1000, abcam), p-IRS1 (1:1000, Thermo https://doi.org/10.3390/pharmaceutics18030349 Fisher Scientific), PI3K (1:2000, proteintech), p-PI3K (1:1000, Thermo Fisher Scientific), AKT (1:5000, proteintech), p-AKT (1:5000, proteintech), GLUT4 (1:2000, proteintech), and β-actin (1/10,000, proteintech). .. Matching horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000, proteintech) were used to evaluate protein expression.

Electrophoresis:

Article Title: Oral Rhizoma Coptis Alkaloids Nanoparticle for Treating Diabetes Through Regulating PI3K/Akt Pathways
Article Snippet: Protein concentration was determined using the BCA protein detection kit (Beyotime, Beijing, China). .. Total protein was separated by SDS-PAGE electrophoresis, transferred to a PVDF membrane blocked with 5% fat-free milk at 4 °C for 60 min, and incubated overnight with primary antibodies IL-1β (1:1000, abcam), IL-6 (1:1000, abcam), TNF-α (1:1000, abcam), IRS1 (1:1000, abcam), p-IRS1 (1:1000, Thermo Fisher Scientific), PI3K (1:2000, proteintech), p-PI3K (1:1000, Thermo Fisher Scientific), AKT (1:5000, proteintech), p-AKT (1:5000, proteintech), GLUT4 (1:2000, proteintech), and β-actin (1/10,000, proteintech). .. Matching horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000, proteintech) were used to evaluate protein expression.

Article Title: Oral Rhizoma Coptis Alkaloids Nanoparticle for Treating Diabetes Through Regulating PI3K/Akt Pathways.
Article Snippet: Protein concentration was determined using the BCA protein detection kit (Beyotime, Beijing, China). .. Total protein was separated by SDS-PAGE electrophoresis, transferred to a PVDF membrane blocked with 5% fat-free milk at 4 ◦C for 60 min, and incubated overnight with primary antibodies IL-1β (1:1000, abcam), IL-6 (1:1000, abcam), TNF-α (1:1000, abcam), IRS1 (1:1000, abcam), p-IRS1 (1:1000, Thermo https://doi.org/10.3390/pharmaceutics18030349 Fisher Scientific), PI3K (1:2000, proteintech), p-PI3K (1:1000, Thermo Fisher Scientific), AKT (1:5000, proteintech), p-AKT (1:5000, proteintech), GLUT4 (1:2000, proteintech), and β-actin (1/10,000, proteintech). .. Matching horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000, proteintech) were used to evaluate protein expression.

Membrane:

Article Title: Oral Rhizoma Coptis Alkaloids Nanoparticle for Treating Diabetes Through Regulating PI3K/Akt Pathways
Article Snippet: Protein concentration was determined using the BCA protein detection kit (Beyotime, Beijing, China). .. Total protein was separated by SDS-PAGE electrophoresis, transferred to a PVDF membrane blocked with 5% fat-free milk at 4 °C for 60 min, and incubated overnight with primary antibodies IL-1β (1:1000, abcam), IL-6 (1:1000, abcam), TNF-α (1:1000, abcam), IRS1 (1:1000, abcam), p-IRS1 (1:1000, Thermo Fisher Scientific), PI3K (1:2000, proteintech), p-PI3K (1:1000, Thermo Fisher Scientific), AKT (1:5000, proteintech), p-AKT (1:5000, proteintech), GLUT4 (1:2000, proteintech), and β-actin (1/10,000, proteintech). .. Matching horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000, proteintech) were used to evaluate protein expression.

Article Title: Oral Rhizoma Coptis Alkaloids Nanoparticle for Treating Diabetes Through Regulating PI3K/Akt Pathways.
Article Snippet: Protein concentration was determined using the BCA protein detection kit (Beyotime, Beijing, China). .. Total protein was separated by SDS-PAGE electrophoresis, transferred to a PVDF membrane blocked with 5% fat-free milk at 4 ◦C for 60 min, and incubated overnight with primary antibodies IL-1β (1:1000, abcam), IL-6 (1:1000, abcam), TNF-α (1:1000, abcam), IRS1 (1:1000, abcam), p-IRS1 (1:1000, Thermo https://doi.org/10.3390/pharmaceutics18030349 Fisher Scientific), PI3K (1:2000, proteintech), p-PI3K (1:1000, Thermo Fisher Scientific), AKT (1:5000, proteintech), p-AKT (1:5000, proteintech), GLUT4 (1:2000, proteintech), and β-actin (1/10,000, proteintech). .. Matching horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000, proteintech) were used to evaluate protein expression.

Blocking Assay:

Article Title: T-2 toxin induces glycometabolic reprogramming in chondrocytes via a MALAT1/p38‑mediated ceRNA regulatory axis.
Article Snippet: T-2 toxin is a widely distributed dietary mycotoxin and a key environmental driver of Kashin-Beck disease (KBD), yet the molecular events linking exposure to cartilage injury remain incompletely defined.. We examined the chondrotoxic mechanism of T-2 toxin through a MALAT1/miR-15b-5p/MAP2K3 competing endogenous RNA (ceRNA) axis across cellular, animal and human levels.. Rat models of graded T-2 toxin exposure, combined with mRNA and miRNA sequencing identified affected pathways and candidate non-coding RNAs.



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