antibodies against gfap (Proteintech)

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Proteintech antibodies against gfap
Antibodies Against Gfap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfap/GFAP+Polyclonal+antibody/pm42902480-466-20-24
Average 96 stars, based on 619 article reviews

antibodies against gfap - by Bioz Stars, 2026-11

96/100 stars

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Article Title: Acute trazodone treatment ameliorates behavioral abnormalities and improves subsequent short‐term memory in a lipopolysaccharide‐induced mouse model of acute brain dysfunction
Article Snippet: Membranes were blocked with 5% skim milk in phosphate‐buffered saline containing 0.1% Tween 20 (PBST) at 4°C overnight and incubated overnight at 4°C with primary antibodies diluted in Can Get Signal Solution 1 (TOYOBO, NKB‐101). .. In the cerebral cortex and hippocampus, the microglial marker ionized calcium‐binding adaptor molecule 1 (Iba1), the astrocyte marker glial fibrillary acidic protein (GFAP), the neuronal marker neuronal nuclei (NeuN), and the inflammatory cytokines IL‐6 and TNF‐α were quantified using primary antibodies against Iba1 (Proteintech, 81728‐1‐RR; 1:2000), GFAP (Proteintech, 16825‐1‐AP; 1:5000), NeuN (Abcam, ab128886; 1:1000), IL‐6 (Proteintech, 29444‐1‐AP; 1:1000), and TNF‐α (Proteintech, 26162‐1‐AP; 1:1000), with β‐actin (Proteintech, 20536‐1‐AP; 1:2000) used as a loading control. .. After washing with Tris‐buffered saline containing 0.1% Tween 20 (TBST; Bio‐Rad, 161‐0781), membranes were incubated with HRP‐conjugated anti‐rabbit IgG (Cell Signaling Technology, 7074; 1:2000) diluted in Can Get Signal Solution 2 (TOYOBO, NKB‐101) for 1 h at room temperature.

Article Title: Dual-responsive PDA-HP hydrogel enables mitochondria-targeted mild photothermal therapy for spinal cord repair
Article Snippet: .. Samples were then incubated overnight at 4 °C with the following primary antibodies: GFAP (astrocyte marker, 1:500, Proteintech, China), Iba1 (microglia marker, 1:500, Proteintech, China), Arg-1 (M2 phenotype marker, 1:500, Proteintech, China), iNOS (M1 phenotype marker, 1:500, Proteintech, China). .. After rinsing, sections were incubated with Alexa Fluor 488- or 594-conjugated secondary antibodies (1:1000, Proteintech, China) for 1 h at room temperature in the dark, followed by counterstaining with DAPI (1 μg/mL, 8 min).

Control:

Incubation:

Article Title: Behavioral and biological alterations following transplantation of ASD-associated gut microbiota in mice.
Article Snippet: .. Membranes were blocked with 5% non-fat milk for 1 h at room temperature, then incubated overnight at 4 ◦C with primary antibodies against IBA-1 (1:2000; Oasis, OB-PRB029), GFAP (1:5000;Oasis, OB-PRB005), andGAPDH (1:5000; Proteintech, 81640- 5-RR). ..

Article Title: Maresin1 Improves Angiogenesis and Cognitive Function by Regulating Perivascular Macrophages in Rats with Chronic Cerebral Hypoperfusion.
Article Snippet: 1 Department of Neurology, Hubei Provincial Clinical Research Center for Dementia and Cognitive Impairment, Zhongnan Hospital of Wuhan University, No.169 Donghu Street, Wuhan 430000, Hubei, China 2 Department of Neurology, Huanggang Central Hospital, Huanggang 438000, Hubei, China 3 Department of Neurology, Minda Hospital of Hubei Minzu University, No. 2 Wufengshan Road, Enshi 445000, Hubei, China Abstract Population aging imposes a substantial dementia burden.. Chronic cerebral hypoperfusion (CCH) serves as a crucial early pathological basis for Alzheimer’s disease (AD) and Vascular Dementia (VaD).. Our previous studies have demonstrated that macrophage-derived docosahexaenoic acid metabolite Maresin1 can ameliorate CCH-induced cognitive impairment, though the underlying angiogenesis-related mechanisms remain to be elucidated.

Article Title: Maternal arsenic exposure induces autism-like behaviors via astrocyte-mediated neuronal apoptosis: Protective effects of Taurine.
Article Snippet: .. Cells were then incubated overnight at 4◦C with the following primary antibodies, C3 (1:200, Proteintech, 21337–1-AP), GFAP (1:200, Proteintech, 60190–1-Ig), p-TrkA (1:200, Affinity, AF7184), p-Akt (1:200, Proteintech, 80455–1-RR), and p-Bad (1:200, Affinity, AF3472). .. After three washes with PBS, cells were mounted with antifade mounting medium containing DAPI (Beyotime, P0131–5ml).

Cell-Signaling:

Article Title: C/EBPβ/AEP pathway mediates synaptic impairments and depression-like phenotypes induced by chronic stress
Article Snippet: .. Antibodies to the following targets were used: Synapsin I (Cell Signaling Technology, 5297), PSD95 (ABclonal, A7889), Synaptophysin (Proteintech, 17785–1-AP), SNAP25 (ABclonal, A2234), Syntaxin (ABclonal, A19813), VAMP2 (ABclonal, A1249), C/EBPβ (Santa Cruz, sc-7962), phospho-C/EBPβ(Thr235) (Cell Signaling Technology, 3084), actived-AEP (Cell Signaling Technology, 93627), Iba-1 (Wako, 019-19741), GFAP (Proteintech, 60190-1-Ig), GAPDH (Proteintech, 60004–1‐Ig), Alexa Fluor 488‐conjugated goat anti‐mouse IgG (Thermo Fisher Scientific, A-11029), Alexa Fluor 594‐conjugated goat anti‐rabbit IgG (Thermo Fisher Scientific, A-32740), HRP‐conjugated goat anti‐mouse IgG (BIO‐RAD, 1706516), HRP‐conjugated goat anti‐rabbit IgG (BIO‐RAD, 1706515). .. The artificial cerebrospinal fluid is composed of the following components: 125mM/L NaCl (Sinopharm Chemical Reagent CO., Ltd, 10019308), 2.5mM/L KCl (Sinopharm Chemical Reagent CO., Ltd, 10016308), 1 3 Article in Press Cellular and Molecular Life Sciences https://doi.org/10.1007/s00018-026-06426-4 25mM/L Glucose (Sigma-Aldrich, G8270), 25mM/L NaHCO3 (Sinopharm Chemical Reagent CO., Ltd, 10018960), 1.25 Mm/L NaH2PO4 (Sinopharm Chemical Reagent CO., Ltd, 20040818), 2mM/L CaCl2 (SigmaAldrich, C4901) and 1mM/L MgCl2 (Sigma-Aldrich, M4880).

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