g6pc2  (Bioss)

Journal: Molecular Medicine Reports

Article Title: Possible association of G6PC2 and MUC6 induced by low‑dose‑rate irradiation in mouse intestine with inflammatory bowel disease

doi: 10.3892/mmr.2024.13251

Figure Lengend Snippet: GO term enrichment analysis of DEGs 24 h after 10 mGy radiation exposure.

Article Snippet: Primary antibodies against ATP1A4 (diluted 1:1,000; cat. no. STJ117451; St John's Laboratory Ltd.), G6PC2 (diluted 1:1,000; cat. no. bs-13386R; BIOSS), MUC6 (diluted 1:1,000; cat. no. 224329; United States Biological) and TRPV6 (diluted 1:1,000; cat. no. ACC-036; Alomone Labs) were incubated at 4°C with consistent rocking overnight.

Techniques: Activation Assay, Cell Differentiation, Migration

Journal: Molecular Medicine Reports

Article Title: Possible association of G6PC2 and MUC6 induced by low‑dose‑rate irradiation in mouse intestine with inflammatory bowel disease

doi: 10.3892/mmr.2024.13251

Figure Lengend Snippet: Common upregulated genes.

Article Snippet: Primary antibodies against ATP1A4 (diluted 1:1,000; cat. no. STJ117451; St John's Laboratory Ltd.), G6PC2 (diluted 1:1,000; cat. no. bs-13386R; BIOSS), MUC6 (diluted 1:1,000; cat. no. 224329; United States Biological) and TRPV6 (diluted 1:1,000; cat. no. ACC-036; Alomone Labs) were incubated at 4°C with consistent rocking overnight.

Techniques:

Journal: Molecular Medicine Reports

Article Title: Possible association of G6PC2 and MUC6 induced by low‑dose‑rate irradiation in mouse intestine with inflammatory bowel disease

doi: 10.3892/mmr.2024.13251

Figure Lengend Snippet: LDR IR increases G6PC2 and MUC6 mRNA and protein expression immediately exposure. (A) Schematic diagram of the experimental procedure. In experiment 1, mice irradiated with sham (0 Gy) or 10 mGy were sacrificed for tissue sampling at 1 or 24 h following LDR IR. Red square indicates radiation exposure time; black circles indicate the times of tissue collection from test animals. Functional enrichment analysis of DEGs. (B) Changes in mRNA expression of four selected genes in the jejunum of mice with LDR IR. Protein expression validation by western blot analysis and temporal changes in protein levels of upregulated genes in 10 mGy-irradiated jejunum. (C) Representative western blotting of ATP1A4, G6PC2, MUC6 and TRPV6 in the jejunum. (D) Bar graphs; expression was normalized to β-actin. Data are expressed as the mean ± SEM (n=3 mice per group). *P<0.05 and **P<0.01 vs. the sham group. LDR IR, low-dose-rate irradiation; G6PC2, glucose-6-phosphatase catalytic subunit 2; MUC6, mucin 6; DEGs, differentially expressed genes; ATP1A4, Na + /K + transporting subunit alpha 4; TRPV6, transient receptor potential cation channel subfamily V member 6.

Article Snippet: Primary antibodies against ATP1A4 (diluted 1:1,000; cat. no. STJ117451; St John's Laboratory Ltd.), G6PC2 (diluted 1:1,000; cat. no. bs-13386R; BIOSS), MUC6 (diluted 1:1,000; cat. no. 224329; United States Biological) and TRPV6 (diluted 1:1,000; cat. no. ACC-036; Alomone Labs) were incubated at 4°C with consistent rocking overnight.

Techniques: Expressing, Irradiation, Sampling, Functional Assay, Western Blot

Journal: Molecular Medicine Reports

Article Title: Possible association of G6PC2 and MUC6 induced by low‑dose‑rate irradiation in mouse intestine with inflammatory bowel disease

doi: 10.3892/mmr.2024.13251

Figure Lengend Snippet: G6PC2 mRNA increases in low dose of LDR IR and repeated LDR IR exposure conditions in the jejunum. (A) Schematic diagram of the experimental procedure. In experiment 2, mice exposed to LDR IR with 0, 10, 100, or 500 mGy or HDR IR with 500 mGy, then sacrificed at 1 or 24 h following IR. Red square indicates radiation exposure time; black circles indicate the times of tissue collection from test animals. Changes in the mRNA expression of G6PC2 in mice exposed to LDR IR. Dose-related changes in G6PC2 mRNA expression level in (B) jejunum and (C) colon. (D) Schematic diagram of the experimental procedure. In experiment 3, mice irradiated with sham, 10 mGy once, or 10 mGy three times were sacrificed for tissue sampling at 1 or 24 h following LDR IR. Red square indicates radiation exposure time; black circles indicate the times of tissue collection from test animals. (E) Repetition-related changes in G6PC2 mRNA expression level in jejunum. Data are expressed as the mean ± SEM (n=3 mice per group). *P<0.05, **P<0.01 and ***P<0.01 vs. the sham group. G6PC2, glucose-6-phosphatase catalytic subunit 2; LDR IR, low-dose-rate irradiation; HDR IR, high-dose-rate irradiation.

Article Snippet: Primary antibodies against ATP1A4 (diluted 1:1,000; cat. no. STJ117451; St John's Laboratory Ltd.), G6PC2 (diluted 1:1,000; cat. no. bs-13386R; BIOSS), MUC6 (diluted 1:1,000; cat. no. 224329; United States Biological) and TRPV6 (diluted 1:1,000; cat. no. ACC-036; Alomone Labs) were incubated at 4°C with consistent rocking overnight.

Techniques: Expressing, Irradiation, Sampling

Journal: Molecular Medicine Reports

Article Title: Possible association of G6PC2 and MUC6 induced by low‑dose‑rate irradiation in mouse intestine with inflammatory bowel disease

doi: 10.3892/mmr.2024.13251

Figure Lengend Snippet: DSS and LDR IR did not alter jejunal morphology but increased G6PC2 and MUC6 mRNA expression. (A) Schematic diagram of the experimental procedure. In experiment 4, DSS-induced IBD model mice irradiated with 10 mGy three times were sacrificed for tissue sampling at 1 or 24 h following LDR IR. Red square indicates radiation exposure time; gray square indicates DSS treatment period; black circles indicate the times of tissue collection from test animals. (B) Representative images of hematoxylin and eosin-stained jejunal sections harvested from vehicle- or DSS-treated mice at 24 h after 10 mGy 3 times LDR IR. Changes in the mRNA expression of (C) G6PC2 and (D) MUC6 in the jejunum of mice exposed to DSS and LDR IR. Data are expressed as the mean ± SEM (n=3 mice per group). *P<0.05, **P<0.01 and ***P<0.001 vs. the sham group; ## P<0.01 and ### P<0.001 vs. the DSS group; ++ P<0.01 and +++ P<0.001 vs. the 10 mGy X3 times group. DSS, dextran sodium sulfate; LDR IR, low-dose-rate irradiation; G6PC2, glucose-6-phosphatase catalytic subunit 2; MUC6, mucin 6; IBD, inflammatory bowel disease.

Article Snippet: Primary antibodies against ATP1A4 (diluted 1:1,000; cat. no. STJ117451; St John's Laboratory Ltd.), G6PC2 (diluted 1:1,000; cat. no. bs-13386R; BIOSS), MUC6 (diluted 1:1,000; cat. no. 224329; United States Biological) and TRPV6 (diluted 1:1,000; cat. no. ACC-036; Alomone Labs) were incubated at 4°C with consistent rocking overnight.

Techniques: Expressing, Irradiation, Sampling, Staining

Journal: Frontiers in Endocrinology

Article Title: A molecular signature for the G6PC3/SLC37A2/SLC37A4 interactors in glioblastoma disease progression and in the acquisition of a brain cancer stem cell phenotype

doi: 10.3389/fendo.2023.1265698

Figure Lengend Snippet: Increased gene expression of G6PC3 , SLC37A2 , and SLC37A4 in clinically-annotated glioblastoma tumor tissues. In silico analysis of transcript levels was performed for (A) three members of the G6PC family (G6PC1, G6PC2, G6PC3) and (B) four members of the SLC37A family (SLC37A1, SLC37A2, SLC37A3, SLC37A4) using RNA extracted from clinical samples from glioblastoma (GBM) and low-grade glioma (LGG) (red boxes) and compared to healthy tissue (grey boxes). (* p < 0.05).

Article Snippet: The following QuantiTect primer sets were provided by QIAGEN: G6PC1 (Hs_G6PC_1_SG QT00031913), G6PC2 (Hs_G6PC2_va.1_SG QT01664152), G6PC3 (Hs_G6PC3_1_SG QT00033453), SLC37A1 (SLC37A1_1_SG QT00073094), SLC37A2 (Hs_SLC37A2_1_SG QT00056203), SLC37A3 (Hs_SLC37A3_1_SG QT00057148), SLC37A4 (Hs_SLC37A4_1_SG QT00024325), GAPDH (Hs_GAPDH_2_SG QT01192646), β-actin (Hs_Actb_2_SG QT01680476) and PPIA (Hs_PPIA_4_SG QT01866137).

Techniques: Expressing, In Silico