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farnesoid x receptor fxr  (Proteintech)


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    Structured Review

    Proteintech farnesoid x receptor fxr
    Farnesoid X Receptor Fxr, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fxr/NR1H4+Antibody/pm42884675-67-2-14
    Average 95 stars, based on 107 article reviews
    farnesoid x receptor fxr - by Bioz Stars, 2026-11
    95/100 stars

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    Tissue:

    Article Title: Hyodeoxycholic acid relieves neuropathic pain by activating farnesoid X receptor signaling
    Article Snippet: .. Tissue sections of each group were blocked with 1 % bovine serum albumin and 10 % donkey serum at room temperature for 1 h and then incubated at 4 °C overnight with primary antibodies for neuronal nuclear protein (NeuN) (1:500, Abcam, ab104224), ionized calcium-binding adapter molecule 1 (IBA-1) (1:500, Abcam, ab5076), glial fibrillary acidic protein (GFAP) (1:500, Millipore, MAB360), FXR (1:200, Proteintech, 25055–1-AP), MMP-2 (1:200, Proteintech, 10373-2-AP), MMP-9 (1:200, Proteintech, 10375-2-AP), PPAR-γ (1:200, Proteintech, 16643–1-AP), NLRP3 (1:200, Immunoway, YT5382), and cluster of differentiation 86 (CD86) (1:200, CST, #91882). ..

    Incubation:

    Article Title: Hyodeoxycholic acid relieves neuropathic pain by activating farnesoid X receptor signaling
    Article Snippet: .. Tissue sections of each group were blocked with 1 % bovine serum albumin and 10 % donkey serum at room temperature for 1 h and then incubated at 4 °C overnight with primary antibodies for neuronal nuclear protein (NeuN) (1:500, Abcam, ab104224), ionized calcium-binding adapter molecule 1 (IBA-1) (1:500, Abcam, ab5076), glial fibrillary acidic protein (GFAP) (1:500, Millipore, MAB360), FXR (1:200, Proteintech, 25055–1-AP), MMP-2 (1:200, Proteintech, 10373-2-AP), MMP-9 (1:200, Proteintech, 10375-2-AP), PPAR-γ (1:200, Proteintech, 16643–1-AP), NLRP3 (1:200, Immunoway, YT5382), and cluster of differentiation 86 (CD86) (1:200, CST, #91882). ..

    Article Title: Quercetin Ameliorates Glucose Metabolism Dysregulation in CUMS Model Rats via the Bile Acid-FXR/TGR5 Axis.
    Article Snippet: .. Membranes were blocked for 2 h at ambient temperature in TBST with 5% (w/v) skim milk and then incubated overnight at 4°C with primary antibodies against the following: FXR (1 : 5000, Proteintech, 25055-1-AP, Wuhan, China), CYP family 7 subfamily A member 1 (CYP7A1) (1 : 5000, Proteintech, 18054-1-AP, Wuhan, China), TGR5 (1 : 1000, Signalway Antibody, #47886, MD, U.S.A.), small heterodimer partner (SHP) (1 : 1000, OriGene, TA332442, Rockville, MD, U.S.A.), and fibroblast growth factor 15 (FGF15) (1 : 1000, Affinity Biosciences, DF2651, Jiangsu, China). .. After three 10 min washes with TBST (Servicebio, G0004, Wuhan, China), blots were probed using secondary antibodies (1 : 10000, Li-cor Biosciences Co., Ltd., 926-32211, Lincoln, NE, U.S.A.) for 1 h at room temperature.

    Article Title: Hexafluoropropylene oxide trimer acid (HFPO-TA) exposure predisposes to MASLD through reprogramming hepatic epigenome and transcriptome.
    Article Snippet: .. The membranes were incubated at room temperature for 1 h with primary antibody against PPARα (1:3000, Proteintech) and FXR (1:3000, Proteintech), followed by incubation with HRP‐conjugated secondary antibodies (1:5000, Proteintech) for 1 h. The protein bands were visualized using ECL reagents and imaged with the Tanon 5200 Multi system (Shanghai, China). .. Statistical analysis was carried out GraphPad Prism (v 9.0.0).

    Article Title: 20(S/R)-ginsenoside Rh1 improves type 2 diabetes via gut microbiota-modulated bile acid signaling and FXR/TGR5-dependent GLP-1 enhancement.
    Article Snippet: .. Frozen ileal Section (5 μm thick) were blocked with 5 % Bovine Serum Albumin (BSA) for 1 h, followed by incubation with primary antibodies against TGR5 (1:100) and FXR (1:100; Proteintech, Wuhan, China) overnight at 4 °C. .. Following primary antibody incubation, the sections were washed and subsequently incubated with Alexa Fluor 594 secondary antibodies (1:250; Invitrogen, Carlsbad, CA, USA) for 1 h at room temperature.

    Article Title: FXR overexpression promotes liver regeneration through inducing FGF2 activation in APAP-induced acute liver injury.
    Article Snippet: Background and purpose Acetaminophen (APAP)-induced hepatotoxicity represents a critical medical emergency due to the rapid progression.. To date, practical and feasible early interventions are limited.. This study aimed to explore whether farnesoid X receptor (FXR, NR1H4) alleviates APAP-induced acute liver injury and whether this effect is mediated by fibroblast growth factor 2 (FGF2) activation to promote liver regeneration.



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