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Promega fugene6 transfection reagent
Fugene6 Transfection Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fugene6/fugene+6/pmc11385518__mmc2-194-10-13
Average 90 stars, based on 1 article reviews
fugene6 transfection reagent - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Cell Culture:

Article Title: Dual role of circulating and mucosal Vδ1 T cells in the control of and contribution to persistent HIV-1 infection.
Article Snippet: .. Generation of HIV-1 viral stock HEK293T cells (ATCC) were seeded and cultured to 50–80% confluency prior to transfection with Fugene6 (Promega) and HIVJR-CSF plasmidDNA (NIHAIDSReagent Program) at a ratio of 3:1. ..

Article Title: Molecular dynamics guided identification of a brighter variant of superfolder Green Fluorescent Protein with increased photobleaching resistance.
Article Snippet: The LifeAct-sfGFP fusion gene was synthesised by Twist Biosciences (see Supporting Information for sequence) and placed in their pTWIST CMV mammalian expression vector. .. Human cervical carcinoma (HeLa) cells (ATCC, UK)were cultured directly onto live cell dishes (Mattek, USA), and allowed to adhere overnight before transfecting using Fugene6 (Promega) according tomanufacturer’s instruction using 1 μg ofDNAper 3 μL Fugene in a volumeof 100 μl ofOptiMEM(Life Technologies,UK). .. Before imaging, cells were washed and the media replaced with Fluorobrite DMEM containing 10% FBS (Life Technologies, UK).

Article Title: Molecular dynamics guided identification of a brighter variant of superfolder Green Fluorescent Protein with increased photobleaching resistance
Article Snippet: The LifeAct-sfGFP fusion gene was synthesised by Twist Biosciences (see Supporting Information for sequence) and placed in their pTWIST CMV mammalian expression vector. .. Human cervical carcinoma (HeLa) cells (ATCC, UK) were cultured directly onto live cell dishes (Mattek, USA), and allowed to adhere overnight before transfecting using Fugene6 (Promega) according to manufacturer’s instruction using 1 μg of DNA per 3 μL Fugene in a volume of 100 μl of OptiMEM (Life Technologies, UK). .. Before imaging, cells were washed and the media replaced with Fluorobrite DMEM containing 10% FBS (Life Technologies, UK).

Transfection:

Article Title: Dual role of circulating and mucosal Vδ1 T cells in the control of and contribution to persistent HIV-1 infection.
Article Snippet: .. Generation of HIV-1 viral stock HEK293T cells (ATCC) were seeded and cultured to 50–80% confluency prior to transfection with Fugene6 (Promega) and HIVJR-CSF plasmidDNA (NIHAIDSReagent Program) at a ratio of 3:1. ..

Article Title: Inherited deficiency of DIAPH1 identifies a DNA double strand break repair pathway regulated by γ-actin.
Article Snippet: .. DR-GFP U-2 OS reporter cells (obtained from Jeremy Stark)71 were transfected with the indicated siRNAs in conjunction with plasmids expressing RFP and the I-SceI endonuclease using FuGene6 (Promega). ..

Article Title: TRPML2 in distinct states reveals the activation and modulation principles of the TRPML family.
Article Snippet: HEK293 cells were seeded onto 6-well plates at a density of 300 k cells per well in DMEM low glucose (Sigma) with 5% FCS (Gemini Bio) and 1% penicillin-streptomycin (Gibco). .. 24 h later, the cells were transfected with either the construct of interest, or empty vector as a control using Fugene6 (Promega). .. 48 h after the transfection, the cells were harvested and rinsed by PBS and lysed with Pierce RIPA buffer (Thermo Scientific) with the addition of EDTA-free protease inhibitor cocktail (Roche) and benzonase (Millipore Sigma).

Article Title: Methods of treating Danon disease
Article Snippet: .. Human fibroblast cells were infected with retrovirus produced in L293 cells transfected with pMXs plasmid DNA (encoding Oct3/4, Sox2, Klf4, and c-Myc, respectively): pUMVC: pCMV-VSV-G (8:8:1) together with FuGENE6 (Promega) according to the manufacturer's protocol. ..

Expressing:

Article Title: Inherited deficiency of DIAPH1 identifies a DNA double strand break repair pathway regulated by γ-actin.
Article Snippet: .. DR-GFP U-2 OS reporter cells (obtained from Jeremy Stark)71 were transfected with the indicated siRNAs in conjunction with plasmids expressing RFP and the I-SceI endonuclease using FuGene6 (Promega). ..

Construct:

Article Title: Enhanced engraftment and immunomodulatory effects of integrin alpha-2-overexpressing mesenchymal stromal cells in lipopolysaccharide-induced acute lung injury.
Article Snippet: .. The mEmerald-integrin-alpha-2 or mEmerald-N1 construct (Addgene, Massachusetts, Watertown, USA) was mixed with FuGENE6 (Promega, Madison, WI, USA) in Opti MEM medium (Thermo Fisher Scientific). ..

Article Title: TRPML2 in distinct states reveals the activation and modulation principles of the TRPML family.
Article Snippet: HEK293 cells were seeded onto 6-well plates at a density of 300 k cells per well in DMEM low glucose (Sigma) with 5% FCS (Gemini Bio) and 1% penicillin-streptomycin (Gibco). .. 24 h later, the cells were transfected with either the construct of interest, or empty vector as a control using Fugene6 (Promega). .. 48 h after the transfection, the cells were harvested and rinsed by PBS and lysed with Pierce RIPA buffer (Thermo Scientific) with the addition of EDTA-free protease inhibitor cocktail (Roche) and benzonase (Millipore Sigma).

Plasmid Preparation:

Article Title: TRPML2 in distinct states reveals the activation and modulation principles of the TRPML family.
Article Snippet: HEK293 cells were seeded onto 6-well plates at a density of 300 k cells per well in DMEM low glucose (Sigma) with 5% FCS (Gemini Bio) and 1% penicillin-streptomycin (Gibco). .. 24 h later, the cells were transfected with either the construct of interest, or empty vector as a control using Fugene6 (Promega). .. 48 h after the transfection, the cells were harvested and rinsed by PBS and lysed with Pierce RIPA buffer (Thermo Scientific) with the addition of EDTA-free protease inhibitor cocktail (Roche) and benzonase (Millipore Sigma).

Article Title: Methods of treating Danon disease
Article Snippet: .. Human fibroblast cells were infected with retrovirus produced in L293 cells transfected with pMXs plasmid DNA (encoding Oct3/4, Sox2, Klf4, and c-Myc, respectively): pUMVC: pCMV-VSV-G (8:8:1) together with FuGENE6 (Promega) according to the manufacturer's protocol. ..

Control:

Article Title: TRPML2 in distinct states reveals the activation and modulation principles of the TRPML family.
Article Snippet: HEK293 cells were seeded onto 6-well plates at a density of 300 k cells per well in DMEM low glucose (Sigma) with 5% FCS (Gemini Bio) and 1% penicillin-streptomycin (Gibco). .. 24 h later, the cells were transfected with either the construct of interest, or empty vector as a control using Fugene6 (Promega). .. 48 h after the transfection, the cells were harvested and rinsed by PBS and lysed with Pierce RIPA buffer (Thermo Scientific) with the addition of EDTA-free protease inhibitor cocktail (Roche) and benzonase (Millipore Sigma).

Infection:

Article Title: Methods of treating Danon disease
Article Snippet: .. Human fibroblast cells were infected with retrovirus produced in L293 cells transfected with pMXs plasmid DNA (encoding Oct3/4, Sox2, Klf4, and c-Myc, respectively): pUMVC: pCMV-VSV-G (8:8:1) together with FuGENE6 (Promega) according to the manufacturer's protocol. ..

Produced:

Article Title: Methods of treating Danon disease
Article Snippet: .. Human fibroblast cells were infected with retrovirus produced in L293 cells transfected with pMXs plasmid DNA (encoding Oct3/4, Sox2, Klf4, and c-Myc, respectively): pUMVC: pCMV-VSV-G (8:8:1) together with FuGENE6 (Promega) according to the manufacturer's protocol. ..



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