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fsp27  (Bioss)


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    Structured Review

    Bioss fsp27
    Fsp27, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fsp27/CIDEC+Polyclonal+Antibody/pmc11071982-204-57-63
    Average 93 stars, based on 5 article reviews
    fsp27 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Interference of a mammalian circRNA regulates lipid metabolism reprogramming by targeting miR-24-3p/Igf2/PI3K-AKT-mTOR and Igf2bp2/Ucp1 axis
    Article Snippet: Briefly, the denatured protein was resolved on the SurePAGE gels (GenScript Corporation, Nanjing, China) and then transferred to a PVDF membrane, followed by sealing of the sealing fluid. .. The membranes were first incubated for 8 h at 4 °C with the matching primary antibodies, then with the secondary antibodies [Goat Anti-Rabbit or Anti-Mouse IgG H&L (HRP), Zen bioscience, Chengdu, China] for 1 h. The antibodies information is as follows: Igf2bp2 (11601-1-AP), Ucp1 (23673-1-AP), PCNA (10205-2-AP), and LPIN1 (27026-1-AP) were purchased from the Proteintech (Wuhan, China); Fsp27 (bs-6796R) was purchased from the Bioss (Beijing, China); PPARG (A0270), CEBPA (A0904), FABP4 (A0232), and SCD (A16429) were purchased from the Abclonal (Wuhan, China); and mTOR (380411), P-mTOR (381557), AKT (382804), P-AKT (310021), PI3K (251221), SREBP1 (347061), IL-1β (511369), TNF-α (346654), β-actin (380624), Igf2 (820814), FASN (31582), and IL-6 (347023) were purchased from Zen bioscience (Chengdu, China). .. The membranes were subjected to an ECL chemiluminescence reagent (HAKATA, Shanghai, China) to detect immunoreactivities.

    Article Title: Interference of a mammalian circRNA regulates lipid metabolism reprogramming by targeting miR-24-3p/Igf2/PI3K-AKT-mTOR and Igf2bp2/Ucp1 axis.
    Article Snippet: White adipose tissue (WAT) is important for regulating the whole systemic energy homeostasis.. Excessive WAT accumulation further contributes to the development of obesity and obesity-related illnesses.. More detailed mechanisms for WAT lipid metabolism reprogramming, however, are still elusive.



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    Figure 1: Depletion of TMEM135 decreases lipid accumulation in the liver of HFD mice and FFA-treated primary hepatocytes and AML12 cells. (AeC) WT and TMEM135KO mice were fed either NCD or HFD for 22 weeks (n ¼ 6 per group). (A) Oil Red O staining of liver tissue section (Original magnification x100). (B) Immunoblots and densitometry for PLIN2, <t>FSP27,</t> and b-actin from the liver tissue of mice. Data represent mean SD, **p < 0.01, *p < 0.05, Two-way ANOVA. (C) Hepatic TG levels. Data represent mean SD, **p < 0.01, Two-way ANOVA. (DeF) Primary hepatocytes were isolated from the livers of WT and TMEM135KO mice and treated with FFA for 12 h. (D) Oil Red O staining and quantification of LD area (Original magnification x40, scale bar 20 mm). More than 100 cells per group in each experiment were measured. Data represent mean SD, ****p < 0.0001, Two-way ANOVA. (E) Immunoblots and densitometry for PLIN2 and b-actin. Data represent mean SD, ****p < 0.0001, ***p < 0.001, Two- way ANOVA. (F) Quantification of TG levels. Data represent mean SD, ****p < 0.0001, Two-way ANOVA. (GeI) sh-Scramble and sh-TMEM135 cells were treated with FFA for 12 h. (G) Oil Red O staining and quantification of LD area (Original magnification x100, scale bar 20 mm). More than 100 cells per group in each experiment were measured. Data represent mean SD, ****p < 0.0001, **p < 0.01, Two-way ANOVA. (H) Immunoblots and densitometry for PLIN2 and b-actin. Data represent mean SD, ****p < 0.0001, Two-way ANOVA. (I) Quantification of TG levels. Data represent mean SD, ****p < 0.0001, **p < 0.01, Two-way ANOVA. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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    Figure 1: Depletion of TMEM135 decreases lipid accumulation in the liver of HFD mice and FFA-treated primary hepatocytes and AML12 cells. (AeC) WT and TMEM135KO mice were fed either NCD or HFD for 22 weeks (n ¼ 6 per group). (A) Oil Red O staining of liver tissue section (Original magnification x100). (B) Immunoblots and densitometry for PLIN2, <t>FSP27,</t> and b-actin from the liver tissue of mice. Data represent mean SD, **p < 0.01, *p < 0.05, Two-way ANOVA. (C) Hepatic TG levels. Data represent mean SD, **p < 0.01, Two-way ANOVA. (DeF) Primary hepatocytes were isolated from the livers of WT and TMEM135KO mice and treated with FFA for 12 h. (D) Oil Red O staining and quantification of LD area (Original magnification x40, scale bar 20 mm). More than 100 cells per group in each experiment were measured. Data represent mean SD, ****p < 0.0001, Two-way ANOVA. (E) Immunoblots and densitometry for PLIN2 and b-actin. Data represent mean SD, ****p < 0.0001, ***p < 0.001, Two- way ANOVA. (F) Quantification of TG levels. Data represent mean SD, ****p < 0.0001, Two-way ANOVA. (GeI) sh-Scramble and sh-TMEM135 cells were treated with FFA for 12 h. (G) Oil Red O staining and quantification of LD area (Original magnification x100, scale bar 20 mm). More than 100 cells per group in each experiment were measured. Data represent mean SD, ****p < 0.0001, **p < 0.01, Two-way ANOVA. (H) Immunoblots and densitometry for PLIN2 and b-actin. Data represent mean SD, ****p < 0.0001, Two-way ANOVA. (I) Quantification of TG levels. Data represent mean SD, ****p < 0.0001, **p < 0.01, Two-way ANOVA. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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    Figure 1: Depletion of TMEM135 decreases lipid accumulation in the liver of HFD mice and FFA-treated primary hepatocytes and AML12 cells. (AeC) WT and TMEM135KO mice were fed either NCD or HFD for 22 weeks (n ¼ 6 per group). (A) Oil Red O staining of liver tissue section (Original magnification x100). (B) Immunoblots and densitometry for PLIN2, <t>FSP27,</t> and b-actin from the liver tissue of mice. Data represent mean SD, **p < 0.01, *p < 0.05, Two-way ANOVA. (C) Hepatic TG levels. Data represent mean SD, **p < 0.01, Two-way ANOVA. (DeF) Primary hepatocytes were isolated from the livers of WT and TMEM135KO mice and treated with FFA for 12 h. (D) Oil Red O staining and quantification of LD area (Original magnification x40, scale bar 20 mm). More than 100 cells per group in each experiment were measured. Data represent mean SD, ****p < 0.0001, Two-way ANOVA. (E) Immunoblots and densitometry for PLIN2 and b-actin. Data represent mean SD, ****p < 0.0001, ***p < 0.001, Two- way ANOVA. (F) Quantification of TG levels. Data represent mean SD, ****p < 0.0001, Two-way ANOVA. (GeI) sh-Scramble and sh-TMEM135 cells were treated with FFA for 12 h. (G) Oil Red O staining and quantification of LD area (Original magnification x100, scale bar 20 mm). More than 100 cells per group in each experiment were measured. Data represent mean SD, ****p < 0.0001, **p < 0.01, Two-way ANOVA. (H) Immunoblots and densitometry for PLIN2 and b-actin. Data represent mean SD, ****p < 0.0001, Two-way ANOVA. (I) Quantification of TG levels. Data represent mean SD, ****p < 0.0001, **p < 0.01, Two-way ANOVA. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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    Image Search Results


    Figure 1: Depletion of TMEM135 decreases lipid accumulation in the liver of HFD mice and FFA-treated primary hepatocytes and AML12 cells. (AeC) WT and TMEM135KO mice were fed either NCD or HFD for 22 weeks (n ¼ 6 per group). (A) Oil Red O staining of liver tissue section (Original magnification x100). (B) Immunoblots and densitometry for PLIN2, FSP27, and b-actin from the liver tissue of mice. Data represent mean SD, **p < 0.01, *p < 0.05, Two-way ANOVA. (C) Hepatic TG levels. Data represent mean SD, **p < 0.01, Two-way ANOVA. (DeF) Primary hepatocytes were isolated from the livers of WT and TMEM135KO mice and treated with FFA for 12 h. (D) Oil Red O staining and quantification of LD area (Original magnification x40, scale bar 20 mm). More than 100 cells per group in each experiment were measured. Data represent mean SD, ****p < 0.0001, Two-way ANOVA. (E) Immunoblots and densitometry for PLIN2 and b-actin. Data represent mean SD, ****p < 0.0001, ***p < 0.001, Two- way ANOVA. (F) Quantification of TG levels. Data represent mean SD, ****p < 0.0001, Two-way ANOVA. (GeI) sh-Scramble and sh-TMEM135 cells were treated with FFA for 12 h. (G) Oil Red O staining and quantification of LD area (Original magnification x100, scale bar 20 mm). More than 100 cells per group in each experiment were measured. Data represent mean SD, ****p < 0.0001, **p < 0.01, Two-way ANOVA. (H) Immunoblots and densitometry for PLIN2 and b-actin. Data represent mean SD, ****p < 0.0001, Two-way ANOVA. (I) Quantification of TG levels. Data represent mean SD, ****p < 0.0001, **p < 0.01, Two-way ANOVA. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Molecular metabolism

    Article Title: TMEM135 deficiency improves hepatic steatosis by suppressing CD36 in a SIRT1-dependent manner.

    doi: 10.1016/j.molmet.2024.102080

    Figure Lengend Snippet: Figure 1: Depletion of TMEM135 decreases lipid accumulation in the liver of HFD mice and FFA-treated primary hepatocytes and AML12 cells. (AeC) WT and TMEM135KO mice were fed either NCD or HFD for 22 weeks (n ¼ 6 per group). (A) Oil Red O staining of liver tissue section (Original magnification x100). (B) Immunoblots and densitometry for PLIN2, FSP27, and b-actin from the liver tissue of mice. Data represent mean SD, **p < 0.01, *p < 0.05, Two-way ANOVA. (C) Hepatic TG levels. Data represent mean SD, **p < 0.01, Two-way ANOVA. (DeF) Primary hepatocytes were isolated from the livers of WT and TMEM135KO mice and treated with FFA for 12 h. (D) Oil Red O staining and quantification of LD area (Original magnification x40, scale bar 20 mm). More than 100 cells per group in each experiment were measured. Data represent mean SD, ****p < 0.0001, Two-way ANOVA. (E) Immunoblots and densitometry for PLIN2 and b-actin. Data represent mean SD, ****p < 0.0001, ***p < 0.001, Two- way ANOVA. (F) Quantification of TG levels. Data represent mean SD, ****p < 0.0001, Two-way ANOVA. (GeI) sh-Scramble and sh-TMEM135 cells were treated with FFA for 12 h. (G) Oil Red O staining and quantification of LD area (Original magnification x100, scale bar 20 mm). More than 100 cells per group in each experiment were measured. Data represent mean SD, ****p < 0.0001, **p < 0.01, Two-way ANOVA. (H) Immunoblots and densitometry for PLIN2 and b-actin. Data represent mean SD, ****p < 0.0001, Two-way ANOVA. (I) Quantification of TG levels. Data represent mean SD, ****p < 0.0001, **p < 0.01, Two-way ANOVA. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: CD36 antibody (#NB400-144) and FSP27 (#NB100-430) were bought from Novus Biological (Minneapolis, MN, USA).

    Techniques: Staining, Western Blot, Isolation