19672 1ap  (Proteintech)

Journal: Poultry Science

Article Title: Construction and mechanism analysis of the sex reversal model of chicken gonadal somatic cells

doi: 10.1016/j.psj.2025.105435

Figure Lengend Snippet: Molecular and cellular characterization of male and female gonadal somatic cells. A. Identification of the mRNA expression level of sex-related genes in male and female gonadal somatic cells by qRT-PCR. * P < 0.05, significant difference; ** P < 0.01, extremely significant difference. B. Detection of the expression changes of sex-related genes in male and female gonadal somatic cells by Western blot. C. Observation of the expression of FOXL2 and SOX9 in male and female gonadal somatic cells by immunofluorescence. Anti- FOXL2 / SOX9 conjugated to FITC was shown as a green fluorescence signal. Nucleus staining with DAPI was shown as a blue signal. Scale bars: 500 μm (upper), 100 μm (lower).

Article Snippet: The male gonadal somatic cells were incubated with SOX9 antibody (1:200, SOX9 Recombinant Rabbit Monoclonal Antibody [SN74-09], Huaan Biotech, diluted in 3 % BSA) and the female gonadal somatic cells were incubated with FOXL2 antibody (262C1a) (1:50, FOXL2 antibody [262C1a]; sc-81275, Santa Cruz).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence, Fluorescence, Staining

Journal: Poultry Science

Article Title: Construction and mechanism analysis of the sex reversal model of chicken gonadal somatic cells

doi: 10.1016/j.psj.2025.105435

Figure Lengend Snippet: Flow cytometry analysis of the expression of FOXL2 and SOX9 in male and female gonadal somatic cells after treatment with FAD (50 nM) or E2 (50 nM, 100 nM). A. The expression level of FOXL2 and SOX9 in male and female gonadal somatic cells after treatment with FAD (50 nM) or E2 (50 nM, 100 nM) for 3d, 4d, or 13d. The horizontal axis represents the green fluorescence signal (FITC) of Anti- FOXL2 conjugated to FITC. The vertical axis represents the red fluorescence signal (PE) of Anti- SOX9 conjugated to ABflo® 594. N = 3. B. Statistical analysis of the expression of SOX9 in female gonadal cells treated with 50 nM FAD for 3 days. C. Statistical analysis of the expression of SOX9 in female gonadal cells treated with 50 nM FAD for 13 days. D. Statistical analysis of the expression of FOXL2 in female gonadal cells treated with 50 nM E2 for 3 days or 100 nM E2 for 4 days. E. Statistical analysis of the expression of FOXL2 in female gonadal cells treated with 50 nM FAD or 100 nM E2 for 13 days. B-E. Statistical analysis of protein expression relative to the corresponding Blank group. ** P < 0.01, *** P < 0.001, **** P < 0.0001; n = 3. All statistical comparisons were performed using Student’s t-test against the respective Blank group.

Article Snippet: The male gonadal somatic cells were incubated with SOX9 antibody (1:200, SOX9 Recombinant Rabbit Monoclonal Antibody [SN74-09], Huaan Biotech, diluted in 3 % BSA) and the female gonadal somatic cells were incubated with FOXL2 antibody (262C1a) (1:50, FOXL2 antibody [262C1a]; sc-81275, Santa Cruz).

Techniques: Flow Cytometry, Expressing, Fluorescence