Journal: Frontiers in Immunology
Article Title: Quantitative bead–based multiplex assay for simultaneous determination of IgG concentrations of pertussis toxin, filamentous hemagglutinin, pertactin, diphtheria, tetanus, Haemophilus influenzae b, and hepatitis B in human serum samples
doi: 10.3389/fimmu.2025.1587567
Figure Lengend Snippet: Dilution linearity of assay in high titre sera samples and MRS for PT, FHA, PRN, DT, TT, and Hep B antigens. (A–F) represents the dilution linearity of four samples for PT, FHA, PRN, DT, TT, and Hep B antigens. The x-axis represents the sample’s dilutions, and the y-axis represents the concentration observed in mIU/ml. (G) represents the dilution linearity data for MRS. Sera samples and MRS shows no loss of dilution integrity over the dilution range. PT, pertussis toxin; FHA, filamentous haemagglutinin; PRN, pertactin; DT, diphtheria toxoid; TT, tetanus toxoid; Hep B, hepatitis B; MRS, multiplex reference standard.
Article Snippet: The commercial ELISA kits for quantification of IgG against PT and FHA antigens were procured from Euroimmun, Germany.
Techniques: Concentration Assay, Multiplex Assay