fgg  (Proteintech)

Journal: Frontiers in Immunology

Article Title: Cryofibrinogen-associated glomerulonephritis with paraproteinemia

doi: 10.3389/fimmu.2025.1576917

Figure Lengend Snippet: Cryoprecipitates detection. (A) The precipitates were detected in patient’s plasma but not in serum under 4°C. (B) The precipitates dissolved under 37°C. (C, D) Monoclonal IgG λ was detected in precipitates coexisting with weak fibrinogen (IFE). (E) Crystal-like precipitates with straight column shape at vertical section and tubules with spiral shape at cross-section (10000×). (F) Gold labeled fibrinogen scattered within the crystal area (IEM, 40000×). (G) Genetic analysis identifed heterozygous c.719-27G>C variant in intron 4 of FGB gene. (H) The relative mRNA expression level of FGB was more than two times higher in the patient comparing with her daughter, followed by FGG and FGA . (I) Treatment and follow-up of fibrinogen level and kidney injury. FGB : fibrinogen beta chain; FGG , fibrinogen gamma chain; FGA , fibrinogen alpha chain; Scr, serum creatinine; ALB, albumin; CTX, cyclophosphamide; Pred, prednisone; UPCR, urinary protein-to-creatinine ratio; Fib, fibrinogen, MIg, monoclonal immunoglobulin.

Article Snippet: The quantitative analysis of fibrinogen alpha chain (FGA), fibrinogen beta chain (FGB), and fibrinogen gamma chain (FGG) mRNA was performed using qRT-PCR on an Applied Biosystems QuantStudio 5 Real-Time PCR System.

Techniques: Clinical Proteomics, Labeling, Variant Assay, Expressing

Journal: Frontiers in Immunology

Article Title: Cryofibrinogen-associated glomerulonephritis with paraproteinemia

doi: 10.3389/fimmu.2025.1576917

Figure Lengend Snippet: The interaction of MIg with fibrinogen. (A) The patient’s serum (first admission without MIg) mixed with plasma from a health control showed granular cyroprecipitates under 4°C. (B) Wholly dissolved mixed blood of panel (A) under 37°C. (C) IFE detection of the mixed blood from panel B showing negative MIg. (D) Serum of the patient (second admission with MIg) mixed with plasma from a health control showed gelatinous cyroprecipitates under 4°C. (E) Partially dissolved mixed blood of panel D under 37°C. (F) IFE detection of blood in panel (E) showed MIg (IgG λ). (G) IFE was negative when IgG including MIg in the serum of her second admission was extracted. (H) The patient’s serum after extraction was mixed with plasma from a health control, and no cryoprecipitates were detected under 4°C. (I) The molecular docking revealed a robust interaction between MIg and fibrinogen. Grey, IgG Fab fragment; Purple, fibrinogen alpha chain; Yellow, the residues involved in the interface; Green, fibrinogen beta chain; Light pink, fibrinogen gamma chain. IFE, immunofixation electrophoresis; MIg, monoclonal immunoglobulin.

Article Snippet: The quantitative analysis of fibrinogen alpha chain (FGA), fibrinogen beta chain (FGB), and fibrinogen gamma chain (FGG) mRNA was performed using qRT-PCR on an Applied Biosystems QuantStudio 5 Real-Time PCR System.

Techniques: Clinical Proteomics, Control, Extraction, Electrophoresis

Journal: Scientific Reports

Article Title: Effect of electromagnetic field radiation on transcriptomic profile and DNA methylation level in pig conceptuses during the peri-implantation period

doi: 10.1038/s41598-025-98918-9

Figure Lengend Snippet: Taq Man Probes used in the validation of the NGS results experiment.

Article Snippet: FGG , Ss04329014_s1.

Techniques: Biomarker Discovery

Journal: Scientific Reports

Article Title: Effect of electromagnetic field radiation on transcriptomic profile and DNA methylation level in pig conceptuses during the peri-implantation period

doi: 10.1038/s41598-025-98918-9

Figure Lengend Snippet: Primers used for quantitative methylation-specific PCR.

Article Snippet: FGG , Ss04329014_s1.

Techniques: Methylation, Amplification