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antifabp5  (R&D Systems)


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    Structured Review

    R&D Systems antifabp5
    Antifabp5, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fabp5/Human%2FMouse%2FRat+FABP5%2FE-FABP+Antibody/pm41865123-142-14-15
    Average 95 stars, based on 36 article reviews
    antifabp5 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    other:

    Article Title: Fatty acid binding protein 5 suppression attenuates obesity-induced hepatocellular carcinoma by promoting ferroptosis and intratumoral immune rewiring.
    Article Snippet: Due to the rise in overnutrition, the incidence of obesity-induced hepatocellular carcinoma (HCC) will continue to escalate; however, our understanding of the obesity to HCC developmental axis is limited.. We constructed a single-cell atlas to interrogate the dynamic transcriptomic changes during hepatocarcinogenesis in mice.. Here we identify fatty acid binding protein 5 (FABP5) as a driver of obesity-induced HCC.

    Staining:

    Article Title: Resolved Psoriasis with Abundant Oleic Acid in Stratum Corneum Exhibits Lower T-Cell-Driven IL-17 Signature.
    Article Snippet: Relapses of psoriasis involve T cells that stem and survive in the skin.. Inherited from previous flares, the tissue-resident memory T cells are epidermal IL-17eproducing CD8þ and IL-22eproducing CD4þ T cells.. Because the capacity of resident memory T cells to take in fatty acids is essential for their residence and function, the surface composition of fatty acids may affect underlying T-cell populations.

    Article Title: Intracellular Lipid Accumulation Drives the Differentiation of Decidual Polymorphonuclear Myeloid-Derived Suppressor Cells via Arachidonic Acid Metabolism
    Article Snippet: .. An Fc receptor blocking solution (BioLegend, San Diego, CA, USA) was added prior to staining with the following antibodies: CD45, CD11b, CD66b, pSTAT3 (BD Biosciences, San Jose, CA, USA), CD33, HLA-DR, CD15, CD3, CD4, CD8, arginase 1 (ARG1), IL6Rα, IFN-γ (BioLegend), inducible nitric oxide synthase (iNOS) (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and FABP5 (R&D Systems). .. The mouse IgG1 and rat IgG2a isotype controls were used (BioLegend).

    Blocking Assay:

    Article Title: Intracellular Lipid Accumulation Drives the Differentiation of Decidual Polymorphonuclear Myeloid-Derived Suppressor Cells via Arachidonic Acid Metabolism
    Article Snippet: .. An Fc receptor blocking solution (BioLegend, San Diego, CA, USA) was added prior to staining with the following antibodies: CD45, CD11b, CD66b, pSTAT3 (BD Biosciences, San Jose, CA, USA), CD33, HLA-DR, CD15, CD3, CD4, CD8, arginase 1 (ARG1), IL6Rα, IFN-γ (BioLegend), inducible nitric oxide synthase (iNOS) (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and FABP5 (R&D Systems). .. The mouse IgG1 and rat IgG2a isotype controls were used (BioLegend).

    Article Title: Fatty acid-binding protein 7 triggers α-synuclein oligomerization in glial cells and oligodendrocytes associated with oxidative stress.
    Article Snippet: We previously show that fatty acid-binding protein 3 (FABP3) triggers α-synuclein (Syn) accumulation and induces dopamine neuronal cell death in Parkinson disease mouse model.. But the role of fatty acid-binding protein 7 (FABP7) in the brain remains unclear.. In this study we investigated whether FABP7 was involved in synucleinopathies.

    Article Title: Fatty acid binding protein 5 suppression attenuates obesity-induced hepatocellular carcinoma by promoting ferroptosis and intratumoral immune rewiring
    Article Snippet: Livers were cryosectioned (10 μm) in the transverse axis and sections were washed with 0.1% Triton X-100 (Sigma; T8787) in phosphate-buffered saline (PBS-T). .. Sections were incubated at 4 °C overnight with FABP5 (R&D systems; AF1476), AFP (Protein-tech; 14550–1-AP), CD68 (Bio-Rad; MCA1957) primary antibodies or Phalloidin-Alexa Fluor 647 membrane dye (Thermo Fisher; A22287) (1:1,000 dilution) after blocking with blocking buffer (5% donkey serum and 0.5% BSA in PBS-T) for 1 h at room temperature (RT). .. Following overnight incubation, sections were stained with Alexa Fluor secondary antibodies (Jackson) for 1 h at RT and immersed in a mounting medium with nuclear fluorescent dye 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Vector Laboratories; H-1200).

    Incubation:

    Article Title: Fatty acid-binding protein 7 triggers α-synuclein oligomerization in glial cells and oligodendrocytes associated with oxidative stress.
    Article Snippet: We previously show that fatty acid-binding protein 3 (FABP3) triggers α-synuclein (Syn) accumulation and induces dopamine neuronal cell death in Parkinson disease mouse model.. But the role of fatty acid-binding protein 7 (FABP7) in the brain remains unclear.. In this study we investigated whether FABP7 was involved in synucleinopathies.

    Article Title: Genetic or pharmacologic blockade of mPGES-2 attenuates renal lipotoxicity and diabetic kidney disease by targeting Rev-Erbα/FABP5 signaling.
    Article Snippet: .. The sections were then incubated with the indicated primary antibody for 12 h at 4 C. The primary antibodies usedwere as follows: FABP5 (AF1476, R&D Systems), ADRP/perilipin 2 (15294-1-AP, Proteintech), Wilms tumor protein (ab89901, Abcam), nephrin (AF3159, R&D systems), synaptopodin (sc-515842, Santa Cruz), and TIM-1/KIM-1/HAVCR (NBP1-76701SS, NOVUS). .. Detection was performed using the following secondary antibodies; Alexa Fluor 488 goat anti-rabbit IgG (AB150077, Abcam), Alexa Fluor 488 donkey anti-mouse IgG (AB150109, Abcam), Alexa Fluor 555 donkey anti-rabbit IgG (AB150074, Abcam) and Alexa Fluor 594 donkey anti-mouse IgG (AB150108, Abcam).

    Article Title: Fatty acid binding protein 5 suppression attenuates obesity-induced hepatocellular carcinoma by promoting ferroptosis and intratumoral immune rewiring
    Article Snippet: Livers were cryosectioned (10 μm) in the transverse axis and sections were washed with 0.1% Triton X-100 (Sigma; T8787) in phosphate-buffered saline (PBS-T). .. Sections were incubated at 4 °C overnight with FABP5 (R&D systems; AF1476), AFP (Protein-tech; 14550–1-AP), CD68 (Bio-Rad; MCA1957) primary antibodies or Phalloidin-Alexa Fluor 647 membrane dye (Thermo Fisher; A22287) (1:1,000 dilution) after blocking with blocking buffer (5% donkey serum and 0.5% BSA in PBS-T) for 1 h at room temperature (RT). .. Following overnight incubation, sections were stained with Alexa Fluor secondary antibodies (Jackson) for 1 h at RT and immersed in a mounting medium with nuclear fluorescent dye 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Vector Laboratories; H-1200).

    Wilms Tumor Assay:

    Article Title: Genetic or pharmacologic blockade of mPGES-2 attenuates renal lipotoxicity and diabetic kidney disease by targeting Rev-Erbα/FABP5 signaling.
    Article Snippet: .. The sections were then incubated with the indicated primary antibody for 12 h at 4 C. The primary antibodies usedwere as follows: FABP5 (AF1476, R&D Systems), ADRP/perilipin 2 (15294-1-AP, Proteintech), Wilms tumor protein (ab89901, Abcam), nephrin (AF3159, R&D systems), synaptopodin (sc-515842, Santa Cruz), and TIM-1/KIM-1/HAVCR (NBP1-76701SS, NOVUS). .. Detection was performed using the following secondary antibodies; Alexa Fluor 488 goat anti-rabbit IgG (AB150077, Abcam), Alexa Fluor 488 donkey anti-mouse IgG (AB150109, Abcam), Alexa Fluor 555 donkey anti-rabbit IgG (AB150074, Abcam) and Alexa Fluor 594 donkey anti-mouse IgG (AB150108, Abcam).

    Membrane:

    Article Title: Fatty acid binding protein 5 suppression attenuates obesity-induced hepatocellular carcinoma by promoting ferroptosis and intratumoral immune rewiring
    Article Snippet: Livers were cryosectioned (10 μm) in the transverse axis and sections were washed with 0.1% Triton X-100 (Sigma; T8787) in phosphate-buffered saline (PBS-T). .. Sections were incubated at 4 °C overnight with FABP5 (R&D systems; AF1476), AFP (Protein-tech; 14550–1-AP), CD68 (Bio-Rad; MCA1957) primary antibodies or Phalloidin-Alexa Fluor 647 membrane dye (Thermo Fisher; A22287) (1:1,000 dilution) after blocking with blocking buffer (5% donkey serum and 0.5% BSA in PBS-T) for 1 h at room temperature (RT). .. Following overnight incubation, sections were stained with Alexa Fluor secondary antibodies (Jackson) for 1 h at RT and immersed in a mounting medium with nuclear fluorescent dye 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Vector Laboratories; H-1200).



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    NPL, DCTPP1, and PLTP significantly affected M2 macrophage polarization and CRC cells tumorigenesis (A) Overlap between M2Gs and M2 macrophage marker genes. (B) Relative expression of CTSD , TUBB , <t>FABP5</t> , PLTP , NPL , and DCTPP1 in M0-like, M1-like, and M2-like macrophages. (C) Immunohistochemical staining of CD163, PLTP, NPL, and DCTPP1 in colorectal cancer tissues, n = 8. (D) Schematic of THP-1-derived M2-like macrophage polarization. (E and F) Proliferation and apoptosis of HCT116 cells cultured with conditioned medium from Ctrl or siNDP macrophages, n = 3. (G and H) Schematic and representative images of Transwell migration and Matrigel invasion assays. (I) Representative flow cytometry plots of CD11b and CD163 expression in macrophages under the indicated treatments, n = 3. (J and K) Ki67 immunofluorescence and quantification in HCT116 cells cultured with conditioned media from the indicated groups, n = 3. Ctrl, control siRNA; PMA-ctrl, PMA-differentiated macrophages without IL-10; siNDP, equal-molar mixture of siRNAs targeting NPL, DCTPP1, and PLTP. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. Scale bars, 100 μm unless otherwise indicated.
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    NPL, DCTPP1, and PLTP significantly affected M2 macrophage polarization and CRC cells tumorigenesis (A) Overlap between M2Gs and M2 macrophage marker genes. (B) Relative expression of CTSD , TUBB , <t>FABP5</t> , PLTP , NPL , and DCTPP1 in M0-like, M1-like, and M2-like macrophages. (C) Immunohistochemical staining of CD163, PLTP, NPL, and DCTPP1 in colorectal cancer tissues, n = 8. (D) Schematic of THP-1-derived M2-like macrophage polarization. (E and F) Proliferation and apoptosis of HCT116 cells cultured with conditioned medium from Ctrl or siNDP macrophages, n = 3. (G and H) Schematic and representative images of Transwell migration and Matrigel invasion assays. (I) Representative flow cytometry plots of CD11b and CD163 expression in macrophages under the indicated treatments, n = 3. (J and K) Ki67 immunofluorescence and quantification in HCT116 cells cultured with conditioned media from the indicated groups, n = 3. Ctrl, control siRNA; PMA-ctrl, PMA-differentiated macrophages without IL-10; siNDP, equal-molar mixture of siRNAs targeting NPL, DCTPP1, and PLTP. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. Scale bars, 100 μm unless otherwise indicated.
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    Lipid-associated macrophages in the normal mouse aorta and five mouse models of aortic dissection (A) Representative immunofluorescence images of aortic sections from control mice and five AD mouse models. Scale bars, 200 μm (low magnification) and 20 μm (high magnification). (B) Quantification of <t>FABP5</t> + CD68 + macrophages normalized to total CD68 + macrophages across different models. Data are presented as mean ± SD. Statistical significance was assessed using one-way ANOVA with multiple comparisons. ∗∗∗∗ p < 0.0001 and ∗∗ p < 0.01; ns, not significant.
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    Lipid-associated macrophages in the normal mouse aorta and five mouse models of aortic dissection (A) Representative immunofluorescence images of aortic sections from control mice and five AD mouse models. Scale bars, 200 μm (low magnification) and 20 μm (high magnification). (B) Quantification of <t>FABP5</t> + CD68 + macrophages normalized to total CD68 + macrophages across different models. Data are presented as mean ± SD. Statistical significance was assessed using one-way ANOVA with multiple comparisons. ∗∗∗∗ p < 0.0001 and ∗∗ p < 0.01; ns, not significant.
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    Image Search Results


    NPL, DCTPP1, and PLTP significantly affected M2 macrophage polarization and CRC cells tumorigenesis (A) Overlap between M2Gs and M2 macrophage marker genes. (B) Relative expression of CTSD , TUBB , FABP5 , PLTP , NPL , and DCTPP1 in M0-like, M1-like, and M2-like macrophages. (C) Immunohistochemical staining of CD163, PLTP, NPL, and DCTPP1 in colorectal cancer tissues, n = 8. (D) Schematic of THP-1-derived M2-like macrophage polarization. (E and F) Proliferation and apoptosis of HCT116 cells cultured with conditioned medium from Ctrl or siNDP macrophages, n = 3. (G and H) Schematic and representative images of Transwell migration and Matrigel invasion assays. (I) Representative flow cytometry plots of CD11b and CD163 expression in macrophages under the indicated treatments, n = 3. (J and K) Ki67 immunofluorescence and quantification in HCT116 cells cultured with conditioned media from the indicated groups, n = 3. Ctrl, control siRNA; PMA-ctrl, PMA-differentiated macrophages without IL-10; siNDP, equal-molar mixture of siRNAs targeting NPL, DCTPP1, and PLTP. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. Scale bars, 100 μm unless otherwise indicated.

    Journal: iScience

    Article Title: M2 macrophage associated genes shape prognosis and tumor progression in human colorectal cancer

    doi: 10.1016/j.isci.2026.116230

    Figure Lengend Snippet: NPL, DCTPP1, and PLTP significantly affected M2 macrophage polarization and CRC cells tumorigenesis (A) Overlap between M2Gs and M2 macrophage marker genes. (B) Relative expression of CTSD , TUBB , FABP5 , PLTP , NPL , and DCTPP1 in M0-like, M1-like, and M2-like macrophages. (C) Immunohistochemical staining of CD163, PLTP, NPL, and DCTPP1 in colorectal cancer tissues, n = 8. (D) Schematic of THP-1-derived M2-like macrophage polarization. (E and F) Proliferation and apoptosis of HCT116 cells cultured with conditioned medium from Ctrl or siNDP macrophages, n = 3. (G and H) Schematic and representative images of Transwell migration and Matrigel invasion assays. (I) Representative flow cytometry plots of CD11b and CD163 expression in macrophages under the indicated treatments, n = 3. (J and K) Ki67 immunofluorescence and quantification in HCT116 cells cultured with conditioned media from the indicated groups, n = 3. Ctrl, control siRNA; PMA-ctrl, PMA-differentiated macrophages without IL-10; siNDP, equal-molar mixture of siRNAs targeting NPL, DCTPP1, and PLTP. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. Scale bars, 100 μm unless otherwise indicated.

    Article Snippet: FABP5 , ABclonal , Cat# A27255; RRID: AB_3740927.

    Techniques: Marker, Expressing, Immunohistochemical staining, Staining, Derivative Assay, Cell Culture, Migration, Flow Cytometry, Immunofluorescence, Control

    Lipid-associated macrophages in the normal mouse aorta and five mouse models of aortic dissection (A) Representative immunofluorescence images of aortic sections from control mice and five AD mouse models. Scale bars, 200 μm (low magnification) and 20 μm (high magnification). (B) Quantification of FABP5 + CD68 + macrophages normalized to total CD68 + macrophages across different models. Data are presented as mean ± SD. Statistical significance was assessed using one-way ANOVA with multiple comparisons. ∗∗∗∗ p < 0.0001 and ∗∗ p < 0.01; ns, not significant.

    Journal: iScience

    Article Title: Cross-species scRNA-seq finds ideal mouse models for aortic dissection mechanisms

    doi: 10.1016/j.isci.2026.115147

    Figure Lengend Snippet: Lipid-associated macrophages in the normal mouse aorta and five mouse models of aortic dissection (A) Representative immunofluorescence images of aortic sections from control mice and five AD mouse models. Scale bars, 200 μm (low magnification) and 20 μm (high magnification). (B) Quantification of FABP5 + CD68 + macrophages normalized to total CD68 + macrophages across different models. Data are presented as mean ± SD. Statistical significance was assessed using one-way ANOVA with multiple comparisons. ∗∗∗∗ p < 0.0001 and ∗∗ p < 0.01; ns, not significant.

    Article Snippet: Briefly, arterial sections were dewaxed and hydrated for antigen repair and 3% BSA closure, and then incubated with primary antibodies against α-SMA (1:1000, ab124964, Abcam), CD68 (1:500, GB113109 , Servicebio), FABP5 (1:500, 12348-1-AP, Protein-tech), CD31 (1:2000, ab182981, Abcam), PDGFRA (1:500, ab203491, Abcam) overnight at 4°C.

    Techniques: Dissection, Immunofluorescence, Control

    Lipid-associated VSMCs in the normal mouse aorta and five mouse models of aortic dissection (A) Representative immunofluorescence images of aortic sections from control mice and five AD mouse models. Scale bars, 200 μm (low magnification) and 20 μm (high magnification). (B) Quantification of FABP5 + VSMCs normalized to total αSMA + VSMCs across different models. Data are presented as mean ± SD. Statistical significance was assessed using one-way ANOVA with multiple comparisons. ∗∗∗∗ p < 0.0001; ns, not significant.

    Journal: iScience

    Article Title: Cross-species scRNA-seq finds ideal mouse models for aortic dissection mechanisms

    doi: 10.1016/j.isci.2026.115147

    Figure Lengend Snippet: Lipid-associated VSMCs in the normal mouse aorta and five mouse models of aortic dissection (A) Representative immunofluorescence images of aortic sections from control mice and five AD mouse models. Scale bars, 200 μm (low magnification) and 20 μm (high magnification). (B) Quantification of FABP5 + VSMCs normalized to total αSMA + VSMCs across different models. Data are presented as mean ± SD. Statistical significance was assessed using one-way ANOVA with multiple comparisons. ∗∗∗∗ p < 0.0001; ns, not significant.

    Article Snippet: Briefly, arterial sections were dewaxed and hydrated for antigen repair and 3% BSA closure, and then incubated with primary antibodies against α-SMA (1:1000, ab124964, Abcam), CD68 (1:500, GB113109 , Servicebio), FABP5 (1:500, 12348-1-AP, Protein-tech), CD31 (1:2000, ab182981, Abcam), PDGFRA (1:500, ab203491, Abcam) overnight at 4°C.

    Techniques: Dissection, Immunofluorescence, Control