Journal: PLoS ONE

Article Title: Allergen-Induced Dermatitis Causes Alterations in Cutaneous Retinoid-Mediated Signaling in Mice

doi: 10.1371/journal.pone.0071244

Figure Lengend Snippet: Systemic and topical OVA sensitization results in inflammation, disturbs epidermal barrier homeostasis, and induces PPARδ target gene expression in skin.

Article Snippet: FABP5 protein levels were determined in protein lysates prepared from whole mouse skin according to the protocol indicated in using the rabbit FABP5 polyclonal antibody purchased from ProteinTech (Chicago, IL).

Techniques: Targeted Gene Expression, Binding Assay

Journal: PLoS ONE

Article Title: Allergen-Induced Dermatitis Causes Alterations in Cutaneous Retinoid-Mediated Signaling in Mice

doi: 10.1371/journal.pone.0071244

Figure Lengend Snippet: ( a ) IL-4 serum levels after systemic with or without additional topical OVA sensitization (n = 8). ( b ) ATRA levels in mouse skin determined by HPLC MS-MS method upon systemic (i.p.) and systemic plus topical (i.p.+e.c.) OVA sensitization (n = 3/group). ( c ) Ratio of Fabp5 vs. Crabp2 expression in the skin of OVA-treated mice (n = 6/group) compared to control mice (PBS i.p.). Data are presented as mean values ± SEM. Statistical significance ( p ) is based on one-way ANOVA followed by Tukey’s multiple comparison test for gene expression results and ELISA data. For HPLC MS-MS results, significance was determined using Student’s t -test.

Article Snippet: FABP5 protein levels were determined in protein lysates prepared from whole mouse skin according to the protocol indicated in using the rabbit FABP5 polyclonal antibody purchased from ProteinTech (Chicago, IL).

Techniques: Tandem Mass Spectroscopy, Expressing, Control, Comparison, Gene Expression, Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Allergen-Induced Dermatitis Causes Alterations in Cutaneous Retinoid-Mediated Signaling in Mice

doi: 10.1371/journal.pone.0071244

Figure Lengend Snippet: ( a ) Fabp5 protein levels in the skin of mice with allergen-induced dermatitis. 150 µg proteins were loaded per lane and beta-actin was used as control for even protein loading. ( b ) Immunohistochemical analysis of Fabp5 protein expression in five-micrometer back skin sections of OVA-sensitized mice. ( c ) ATRA-induced nuclear receptor-mediated signaling pathways depending on the predominant cellular transport protein.

Article Snippet: FABP5 protein levels were determined in protein lysates prepared from whole mouse skin according to the protocol indicated in using the rabbit FABP5 polyclonal antibody purchased from ProteinTech (Chicago, IL).

Techniques: Control, Immunohistochemical staining, Expressing, Protein-Protein interactions

Journal: Cancers

Article Title: FABP5 Inhibition against PTEN -Mutant Therapy Resistant Prostate Cancer.

doi: 10.3390/cancers16010060

Figure Lengend Snippet: Figure 3. (A), Left: Cell viability curves of the indicated prostate cancer cell lines treated with increasing concentrations of SBFI-103 for 72 h (n = 3 biologically independent samples). Data are mean ± s.d. Right-top: cell viability curves of RCaP cells treated with increasing concentrations of SBFI-103 for the indicated time points (n = 4 biologically independent samples). Right-bottom: percent cell death indicated by propidium iodide uptake of RCaP cells treated with increasing concentrations of SBFI-103 (n = 4 biologically independent samples). Data are mean ± s.d. (B). Left, cell viability curves of RCaP cells treated with increasing concentrations of 3 TAME inhibitors of FABP5 for 72 h (n = 3 biologically independent samples). Right: chemical structures and IC50 values of the TAME inhibitors.

Article Snippet: Standard western blotting procedures were followed, and the following antibodies were used: β-ACTIN (Sigma–Aldrich, A3854), FABP5 (Cell Signaling Technology (Danvers, MA, USA), 39926), AR (Santa Cruz Biotechnology (Dallas, TX, USA), sc-7305), FKBP5 (Cell Signaling Technology, 12210S), NKX3.1 (Santa Cruz Biotechnology, sc-393190), β-Tubulin (Cell Signaling Technology, 2146), and Vinculin (Cell Signaling Technology, 13901).

Techniques:

Journal: Cancers

Article Title: FABP5 Inhibition against PTEN -Mutant Therapy Resistant Prostate Cancer.

doi: 10.3390/cancers16010060

Figure Lengend Snippet: Figure 5. (A), Immunohistochemistry staining for MMP9 in tumors from 12 animals (vehicle, n = 4; SBFI-103 at 20 mg/kg, n = 4; SBFI-103 at 40 mg/kg, n = 4). Scale: panel width = 450 µm. (B), Quan- tification of MMP9-positive cancer cell fractions for the three trial arms. Individual data points show fraction of MMP9-positive cells per field divided by RFP-positive cells per field (see also Supplementary Figure S3B). Bars show mean ± s.d. p values were calculated using two-way ANOVA with Tukey’s post hoc test. ** p < 0.01, **** p < 0.0001. (C), fraction of change in total animal weights over trial period. (D), Model for inhibition of FABP5-mediated survival and its blockade with SBFI compounds. Green, active FABP5, red SBFI-inactivated FABP5. FA denotes a fatty acid ligand.

Article Snippet: Standard western blotting procedures were followed, and the following antibodies were used: β-ACTIN (Sigma–Aldrich, A3854), FABP5 (Cell Signaling Technology (Danvers, MA, USA), 39926), AR (Santa Cruz Biotechnology (Dallas, TX, USA), sc-7305), FKBP5 (Cell Signaling Technology, 12210S), NKX3.1 (Santa Cruz Biotechnology, sc-393190), β-Tubulin (Cell Signaling Technology, 2146), and Vinculin (Cell Signaling Technology, 13901).

Techniques: Immunohistochemistry, Staining, Inhibition

Journal: iScience

Article Title: Astrocytic FABP5 mediates retrograde endocannabinoid transport at central synapses

doi: 10.1016/j.isci.2025.112342

Figure Lengend Snippet:

Article Snippet: Hippocampal sections (30 μm) were incubated with the following primary antisera: rabbit anti-FABP5 (BioVendor R&D, RD181060100), goat anti-FABP5 (R&D Systems Inc, #AF1476), mouse anti-NeuN (Millipore, MAB377), rabbit anti-NeuN (Abcam, #AB104225), and mouse anti-s100β (Sigma, #S2532).

Techniques: Virus, Recombinant, Software, Imaging, Transfection, Real-time Polymerase Chain Reaction, SYBR Green Assay, Mass Spectrometry, Control, Microscopy

Journal: International Journal of Biological Sciences

Article Title: Macrophage NEDD4L restrains liver fibrosis by preventing scar-associated macrophage expansion via ubiquitination of phospho-SMAD3

doi: 10.7150/ijbs.126649

Figure Lengend Snippet: Myeloid cell-specific knockout of Nedd4l promotes scar-associated macrophage (SAM) differentiation during liver fibrosis. (A) Analysis of Nedd4l expression in liver macrophages from patients with liver fibrosis by analyzing single-cell sequencing database (Liver Cell Atlas: https://singlecell.broadinstitute.org ). (B) Analysis of Nedd4l expression in SAMs from patients with liver fibrosis by analyzing liver single-cell sequencing database (Liver Cell Atlas: https://singlecell.broadinstitute.org ). (C) Representative immunofluorescence images of IBA1 (red), FABP5 (green), and DAPI (white) (Scale bar: 10 μm) in the livers of CCl 4 -treated Nedd4l f/f and Nedd4l ΔMye mice. The number of positive cells was quantified. (D) The hepatic expression of SAM-related profibrotic genes were analyzed by RT-qPCR in liver tissues from oil or CCl 4 -treated Nedd4l f/f and Nedd4l ΔMye mice. (E) Representative images of CD163 staining (Scale bar: 100 μm) and TLR1 staining (Scale bar: 200 μm) from CCl 4 -treated Nedd4l f/f and Nedd4l ΔMye mice are shown. The number of positive cells was quantified. (F) Representative immunofluorescence images of IBA1 (red), FABP5 (green), and DAPI (white) (Scale bar: 10 μm) in the livers of CDAHFD-fed Nedd4l f/f and Nedd4l ΔMye mice are shown. The number of positive cells was quantified. (G) The hepatic expression of SAM-related profibrotic genes were analyzed by RT-qPCR in liver tissues from CDAHFD-fed Nedd4l f/f and Nedd4l ΔMye mice. (H) Representative images of FABP5 staining (Scale bar: 100 μm), TREM2 staining (Scale bar: 100 μm), CD9 staining (Scale bar: 100 μm), and CD206 staining (Scale bar: 100 μm) from CDAHFD-fed Nedd4l f/f and Nedd4l ΔMye mice are shown. The number of positive cells was quantified. Values represent mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The following primary antibodies were used: NEDD4L (Cell Signaling Technology, catalog 4013, Danvers, MA, USA), IBA1 (catalog No. MABN92, Merck KGaA, Darmstadt, Germany), CD206 (ABclonal Technology, catalog A21014, Wuhan, China), FABP5 (ABclonal Technology, catalog A27255, Wuhan, China).

Techniques: Knock-Out, Expressing, Single Cell, Sequencing, Immunofluorescence, Quantitative RT-PCR, Staining

Journal: Developmental biology

Article Title: Dynamic regulation of retinoic acid-binding proteins in developing, adult and neoplastic skin reveals roles for beta-catenin and Notch signalling.

doi: 10.1016/j.ydbio.2008.08.034

Figure Lengend Snippet: Fig. 2. Expression patterns of FABP5 in developing and adult skin. (a–e, g, k, l, m) Embryonic or postnatal back skin labelled with FABP5 (green) and K14 (red; a–e, l, m) or Ki67 (red; g, k) antibodies. (f) Embryonic whisker follicle labelled with FABP5 and counterstained with DAPI. (h) qRT-PCR data showing relative amounts of FABP5 mRNA in back skin derived from embryonic and neonatal mice. Each error bar represents the standard error of the mean of values from 3 mice. (i, j) Wholemounts of back or tail epidermis labelled with FABP5 (green) and K14 (red) antibodies. (n) Back skin immunolabelled with FABP5 (green) and CRABP1 (red) antibodies. Embryonic and postnatal ages are indicated. Dashed lines demarcate (a–d) the epidermal–dermal interface or (f, g, i) hair follicles. Arrows in panels d, f, g, i and j indicate examples of FABP5-expressing cell clusters at the base of primary hair follicles, which represent developing SG. Arrow in panel k points to a population of FABP5-negative/Ki67-positive cells at the base of SG, likely to represent SG progenitors. Scale bars=50 μm (a–f, l, n); 25 μm (k, m); or 150 μm (g, i, j).

Article Snippet: Primary antibodies used were: rabbit anti-mouse K14 (Covance), mouse anti-mouse K14, mouse anti-CRABP1 (Sigma), rabbit anti-CRABP2 (Proteintech, Inc.), biotinylated goat anti-mouse FABP5 (R and D Systems), and rat antimouse Ki67 (DakoCytomation).

Techniques: Expressing, Whisker Assay, Quantitative RT-PCR, Derivative Assay

Journal: Developmental biology

Article Title: Dynamic regulation of retinoic acid-binding proteins in developing, adult and neoplastic skin reveals roles for beta-catenin and Notch signalling.

doi: 10.1016/j.ydbio.2008.08.034

Figure Lengend Snippet: Fig. 3. Expression patterns of RA-binding proteins in skin papillomas and SCC. (a–c) papillomas and panels (d–f) SCC were labelled with panels (a, d) CRABP1 (green) and K14 (red) antibodies; (b, e) CRABP2 (green) and K14 (red) antibodies; (c, f) FABP5 (green) and K14 (red) antibodies. Dashed lines in panels a–c highlight two remnant hair follicles and their associated SG. Scale bars=100 μm.

Article Snippet: Primary antibodies used were: rabbit anti-mouse K14 (Covance), mouse anti-mouse K14, mouse anti-CRABP1 (Sigma), rabbit anti-CRABP2 (Proteintech, Inc.), biotinylated goat anti-mouse FABP5 (R and D Systems), and rat antimouse Ki67 (DakoCytomation).

Techniques: Expressing, Binding Assay

Journal: Developmental biology

Article Title: Dynamic regulation of retinoic acid-binding proteins in developing, adult and neoplastic skin reveals roles for beta-catenin and Notch signalling.

doi: 10.1016/j.ydbio.2008.08.034

Figure Lengend Snippet: Fig. 4. RA is a positive regulator of CRABP1, CRABP2 and FABP5 expression. (a, b) Acetone- or RA-treated back skin immunolabelled with Ki67 and counterstained with haematoxylin. (c–e) qRT-PCR data showing the relative amounts of specific mRNAs in back skin of acetone-treated or RA-treated mice. Each error bar represents the standard error of the mean of values derived from five mice. (f–k) Acetone or RA-treated back skin labelled with K14 (red) and CRABP1 (green; f, i), CRABP2 (green; g, j), or FABP5 (green; h, k). Scale bars=150 μm.

Article Snippet: Primary antibodies used were: rabbit anti-mouse K14 (Covance), mouse anti-mouse K14, mouse anti-CRABP1 (Sigma), rabbit anti-CRABP2 (Proteintech, Inc.), biotinylated goat anti-mouse FABP5 (R and D Systems), and rat antimouse Ki67 (DakoCytomation).

Techniques: Expressing, Quantitative RT-PCR, Derivative Assay

Journal: Developmental biology

Article Title: Dynamic regulation of retinoic acid-binding proteins in developing, adult and neoplastic skin reveals roles for beta-catenin and Notch signalling.

doi: 10.1016/j.ydbio.2008.08.034

Figure Lengend Snippet: Fig. 5. Inhibition of Wnt/β-catenin signalling leads to upregulation of RA-binding-protein expression. (a–d) Back skin of wild type and K14ΔNLef1 (ΔNLef1) mice labelled with antibodies against panels a, c FABP5 (green) and CRABP1 (red), or panels b, d CRABP2 (green) and K14 (red), and counterstained with DAPI. Ages of mice in days are shown. (e, f) Wholemounts of tail epidermis labelled with anti-FABP5. Dashed lines (c, d) demarcate remnant hair follicles from which cysts have developed. Scale bars=100 μm (a–d), or 250 μm (e, f). (g) qRT-PCR data showing the relative amounts of CRABP1, CRABP2 or FABP5 mRNA in back skin derived from wild type and ΔNLef1 mice. Each error bar represents the standard error of the mean of values derived from four mice.

Article Snippet: Primary antibodies used were: rabbit anti-mouse K14 (Covance), mouse anti-mouse K14, mouse anti-CRABP1 (Sigma), rabbit anti-CRABP2 (Proteintech, Inc.), biotinylated goat anti-mouse FABP5 (R and D Systems), and rat antimouse Ki67 (DakoCytomation).

Techniques: Inhibition, Binding Assay, Expressing, Quantitative RT-PCR, Derivative Assay

Journal: Developmental biology

Article Title: Dynamic regulation of retinoic acid-binding proteins in developing, adult and neoplastic skin reveals roles for beta-catenin and Notch signalling.

doi: 10.1016/j.ydbio.2008.08.034

Figure Lengend Snippet: Fig. 6. Effects of β-catenin on expression of RA-binding proteins. (a–k) Back skin from wild type and K14ΔNβ-cateninER (Beta-catenin ER) mice treated for 7 or 14 days with 4OHT. (a, c, e, g, h) Labelling with CRABP1 (red) and FABP5 (green). (b, d, f, i) Labelling with CRABP2. (a–f) DAPI nuclear counterstain is shown in blue. (j, k) Labelling with Ki67 antibody (red) and FABP5 (green; j) or CRABP2 (green; k). In panels f–k, dashed lines demarcate ectopic follicles (EF) and their associated DP. In panels g, h, and i, finer dotted lines demarcate the original hair follicles (HF) and SG. Scale bars=100 μm (a–f); 50 μm (g–i); or 165 μm (j, k). (l) qRT-PCR data showing the relative amounts of CRABP1, CRABP2 or FABP5 mRNA in back skin derived from 4OHT-treated wild type and β-catenin ER mice. Each error bar represents the standard error of the mean of values derived from 3 mice.

Article Snippet: Primary antibodies used were: rabbit anti-mouse K14 (Covance), mouse anti-mouse K14, mouse anti-CRABP1 (Sigma), rabbit anti-CRABP2 (Proteintech, Inc.), biotinylated goat anti-mouse FABP5 (R and D Systems), and rat antimouse Ki67 (DakoCytomation).

Techniques: Expressing, Binding Assay, Quantitative RT-PCR, Derivative Assay

Journal: Developmental biology

Article Title: Dynamic regulation of retinoic acid-binding proteins in developing, adult and neoplastic skin reveals roles for beta-catenin and Notch signalling.

doi: 10.1016/j.ydbio.2008.08.034

Figure Lengend Snippet: Fig. 7. Notch signalling positively regulates expression of RA-binding proteins. (a) qRT- PCR data showing the relative amounts of CRABP1, CRABP2 or FABP5 mRNA in back skin derived from 4OHT-treated wild type and Notch ER mice. Each error bar represents the standard error of the mean of values derived from 3 mice. (b–e) Back skin from wild type and K14NICDOPER (Notch ER) mice treated with 4OHT for 14 days. (b, d) labelled with FABP5 (green) and CRABP1 (red) antibodies and counterstained with DAPI. (c, e) Labelled with CRABP2 (green) and K14 (red) antibodies and counterstained with DAPI. Scale bars=100 μm.

Article Snippet: Primary antibodies used were: rabbit anti-mouse K14 (Covance), mouse anti-mouse K14, mouse anti-CRABP1 (Sigma), rabbit anti-CRABP2 (Proteintech, Inc.), biotinylated goat anti-mouse FABP5 (R and D Systems), and rat antimouse Ki67 (DakoCytomation).

Techniques: Expressing, Binding Assay, Quantitative RT-PCR, Derivative Assay

Journal: Developmental biology

Article Title: Dynamic regulation of retinoic acid-binding proteins in developing, adult and neoplastic skin reveals roles for beta-catenin and Notch signalling.

doi: 10.1016/j.ydbio.2008.08.034

Figure Lengend Snippet: Fig. 8. Schematic summary of the dynamic regulation of RA-binding proteins in skin. (a) Changes in patterns of CRABP1, CRABP2 and FABP5 expression between E13.5 and P5 stages of skin development. Drawing is based on Fuchs, 2008. (b) Changes in patterns of CRABP1, CRABP2 and FABP5 expression in response to epidermal expression of ΔNLef1, β-catenin ER or Notch ER transgenes. Note that the expression patterns in β-catenin- induced anagen follicles are the same as in wild type anagen follicles.

Article Snippet: Primary antibodies used were: rabbit anti-mouse K14 (Covance), mouse anti-mouse K14, mouse anti-CRABP1 (Sigma), rabbit anti-CRABP2 (Proteintech, Inc.), biotinylated goat anti-mouse FABP5 (R and D Systems), and rat antimouse Ki67 (DakoCytomation).

Techniques: Binding Assay, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Isolation and Characterization of Squamous Cell Carcinoma-Derived Stem-like Cells: Role in Tumor Formation

doi: 10.3390/ijms141019540

Figure Lengend Snippet: RAD and NRAD-derived tumor characterization. ( A ) Mitotic Index representing the number of cells undergoing mitosis over total cells were counted in RAD and NRAD-derived tumors ** p < 0.01; ( B ) Survivin and Ki67 expression in RAD and NRAD-derived tumors by immunohistochemistry. Scale bar = 200 μm; ( C ) K10, E-FABP and involucrin expression in RAD and NRAD-derived tumors by immunohistochemistry. Scale bar = 200 μm.

Article Snippet: Membranes containing protein were incubated overnight at 4 °C with the following antibodies: anti-survivin rabbit polyclonal (1:1000; Novus Biologicals, Bloomington, MN, USA), or goat anti-human FABP5 (1:1000; R&D Systems, Inc. Minneapolis, MN, USA), or mouse mAB anti-human involucrin (1:6000; Sigma-Aldrich, St. Louis, MO, USA), or anti-human β 1 -integrin (1:500; Santa Cruz Biotechnology, inc. Santa Cruz, CA, USA) or anti-human β-actin (1:5000; Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Derivative Assay, Expressing, Immunohistochemistry

Journal: Journal of Inflammation Research

Article Title: Serum Proteomic Analysis Revealed Biomarkers for Eosinophilic Chronic Rhinosinusitis with Nasal Polyps Pathophysiology

doi: 10.2147/JIR.S444280

Figure Lengend Snippet: The Up-Regulated and Down-Regulated Top 5 Proteins Between the Two Groups

Article Snippet: Mannose receptor C-type 1 (MRC1) kits (Cat: CSB-E09961h), cadherin 13 (CDH13) kits (Cat: CSB-E13817h), cluster of differentiation 5 antigen-like (CD5L) kits (Cat: CSB-E13423h), matrix metalloproteinase-2 (MMP2) kits (Cat: CSB-E04675h), plasma protease C1 inhibitor (SERPING1) kits (Cat: CSB-EL021086HU), insulin-like growth factor-binding protein 5 (IGFBP5) kits (Cat: CSB-EL010901HU), tripartite motif-containing protein 28 (TRIM28) kits (Cat: CSB-EL024502HU), and fatty acid-binding protein 5 (FABP5) kits (Cat: CSB-EL007946HU) were purchased from Cusabio (Wuhan, China).

Techniques: Clinical Proteomics

Journal: Journal of Inflammation Research

Article Title: Serum Proteomic Analysis Revealed Biomarkers for Eosinophilic Chronic Rhinosinusitis with Nasal Polyps Pathophysiology

doi: 10.2147/JIR.S444280

Figure Lengend Snippet: Validation of the top 5 up and down-regulated proteins in an independent validation cohort. ( A – E ) comparison of serum MRC1, CDH13, LTA4H, CD5L and MMP2 concentrations between the eCRSwNP and neCRSwNP groups. ( F – J ) comparison of serum SERPING1, IGFBP5, TRIM28, CHL1 and FABP5 levels between the eCRSwNP and neCRSwNP groups. *P<0.05; **P<0.01; ***P<0.001.

Article Snippet: Mannose receptor C-type 1 (MRC1) kits (Cat: CSB-E09961h), cadherin 13 (CDH13) kits (Cat: CSB-E13817h), cluster of differentiation 5 antigen-like (CD5L) kits (Cat: CSB-E13423h), matrix metalloproteinase-2 (MMP2) kits (Cat: CSB-E04675h), plasma protease C1 inhibitor (SERPING1) kits (Cat: CSB-EL021086HU), insulin-like growth factor-binding protein 5 (IGFBP5) kits (Cat: CSB-EL010901HU), tripartite motif-containing protein 28 (TRIM28) kits (Cat: CSB-EL024502HU), and fatty acid-binding protein 5 (FABP5) kits (Cat: CSB-EL007946HU) were purchased from Cusabio (Wuhan, China).

Techniques: Biomarker Discovery, Comparison