Journal: Reviews in Cardiovascular Medicine

Article Title: Enhancer of Zeste Homolog 2 Inhibition Attenuates Atrial Fibrillation Post-Myocardial Infarction Through Reduced Atrial Fibrosis

doi: 10.31083/RCM51343

Figure Lengend Snippet: MI induces atrial fibrosis and upregulates the EZH2/H3K27me3 signaling in mice . (A,B) Representative Masson staining images (A) and quantitative analysis (B) of atrial fibrosis in the sham and MI groups ( n = 5). Scale bar = 0.04 mm under 20× field of view. (C–E) Western blot analysis and quantitative analysis of the expression levels of collagen I, α-SMA, and TGF-β1 in the atria of mice from the Sham and MI groups ( n = 6). (F) qPCR analysis of the expression levels of Ezh2 mRNA in the atria of mice from the Sham and MI groups ( n = 6). (G,H) Western blot analysis and quantitative analysis of the expression levels of EZH2 ( n = 6) and H3K27me3 ( n = 9) in the atria of mice from the Sham and MI groups. Sham, Sham operation group; MI, myocardial infarction group; α-SMA, alpha-smooth muscle actin; TGF-β1, transforming growth factor-beta1; EZH2, Enhancer of Zeste Homolog 2; H3K27me3, trimethylated Histone H3 at Lysine 27. Values are displayed as mean ± standard error of the mean.

Article Snippet: The antibodies utilized in this study comprised the following: Enhancer of Zeste Homolog 2 (EZH2, 5246S, CST, USA), trimethylated Histone H3 at Lysine 27 (H3K27me3, 9733S, CST, USA), Histone 3 (H3, 9715S, CST, USA), collagen I (14695-1-AP, Proteintech, USA), Transforming Growth Factor-beta1 (TGF-β1, 81746-2-RR, Proteintech, USA), Alpha-Smooth Muscle Actin (α-SMA, 14395-1-AP, Proteintech, USA), and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH, 60004-1-lg, Proteintech, USA).

Techniques: Staining, Analysis, Western Blot, Expressing

Journal: Reviews in Cardiovascular Medicine

Article Title: Enhancer of Zeste Homolog 2 Inhibition Attenuates Atrial Fibrillation Post-Myocardial Infarction Through Reduced Atrial Fibrosis

doi: 10.31083/RCM51343

Figure Lengend Snippet: EZH2 inhibitor GSK126 ameliorates post-MI AF . (A–C) Western blot analysis and quantitative analysis of the expression levels of EZH2 and H3K27me3 in the atria of mice from the MI + Vehicle and MI + GSK126 groups ( n = 6). (D) Representative ECG of mice in the MI + Vehicle and MI + GSK126 groups under resting state and AF-induced state. (E–H) Quantitative analysis of P wave duration (E) and PR interval (F) under resting state, and comparison of AF induction rate (G) and duration time (H) under AF-induced state in the two groups ( n = 7). MI + Vehicle, myocardial infarction with vehicle control group; MI + GSK126, myocardial infarction group treated with GSK126; EZH2, Enhancer of Zeste Homolog 2; H3K27me3, trimethylated Histone H3 at Lysine 27. Values are displayed as mean ± standard error of the mean, except for (G) where values are n .

Article Snippet: The antibodies utilized in this study comprised the following: Enhancer of Zeste Homolog 2 (EZH2, 5246S, CST, USA), trimethylated Histone H3 at Lysine 27 (H3K27me3, 9733S, CST, USA), Histone 3 (H3, 9715S, CST, USA), collagen I (14695-1-AP, Proteintech, USA), Transforming Growth Factor-beta1 (TGF-β1, 81746-2-RR, Proteintech, USA), Alpha-Smooth Muscle Actin (α-SMA, 14395-1-AP, Proteintech, USA), and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH, 60004-1-lg, Proteintech, USA).

Techniques: Western Blot, Analysis, Expressing, Comparison, Control

Journal: Reviews in Cardiovascular Medicine

Article Title: Enhancer of Zeste Homolog 2 Inhibition Attenuates Atrial Fibrillation Post-Myocardial Infarction Through Reduced Atrial Fibrosis

doi: 10.31083/RCM51343

Figure Lengend Snippet: Inhibition of EZH2 attenuates atrial fibrosis post-MI . (A,B) Representative Masson staining images (A) and quantitative analysis (B) of atrial fibrosis in the MI + Vehicle and MI + GSK126 groups ( n = 5). Scale bar = 0.04 mm under 20× field of view. (C–E) Western blot analysis and quantitative analysis of the expression levels of collagen I, α-SMA, and TGF-β1 in the atria of mice from the MI + Vehicle and MI + GSK126 groups ( n = 6). MI + Vehicle, myocardial infarction with vehicle control group; MI + GSK126, myocardial infarction group treated with GSK126; EZH2, Enhancer of Zeste Homolog 2; α-SMA, alpha-smooth muscle actin; TGF-β1, transforming growth factor-beta1. Values are displayed as mean ± standard error of the mean.

Article Snippet: The antibodies utilized in this study comprised the following: Enhancer of Zeste Homolog 2 (EZH2, 5246S, CST, USA), trimethylated Histone H3 at Lysine 27 (H3K27me3, 9733S, CST, USA), Histone 3 (H3, 9715S, CST, USA), collagen I (14695-1-AP, Proteintech, USA), Transforming Growth Factor-beta1 (TGF-β1, 81746-2-RR, Proteintech, USA), Alpha-Smooth Muscle Actin (α-SMA, 14395-1-AP, Proteintech, USA), and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH, 60004-1-lg, Proteintech, USA).

Techniques: Inhibition, Staining, Analysis, Western Blot, Expressing, Control

Journal: BMC Cancer

Article Title: Investigation of the significance and role of androgen receptor expression in basal/squamous type urothelial carcinoma cell line J82 cells

doi: 10.1186/s12885-026-16533-x

Figure Lengend Snippet: Detection of protein-protein interaction between AR and EZH2 by co-IP-western blotting

Article Snippet: The dilutions of primary antibodies used in this study were as follows: FLAG (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 1:10000; GAPDH (Proteintech, Tokyo, Japan), 1:20000; p21 (Proteintech, Tokyo, Japan), 1:10000; EZH2 (Proteintech, Tokyo, Japan), 1:10000.

Techniques: Co-Immunoprecipitation Assay, Western Blot