esrrb  (Proteintech)

Journal: International Journal of Molecular Sciences

Article Title: Calcium and Cadmium Activate ESRRB to Mediate Cell Stemness and Pluripotency

doi: 10.3390/ijms27010231

Figure Lengend Snippet: Effects of calcium and cadmium on ESRRB-regulated genes in HEK293T cells. ( A – E ), Effects of calcium on the induction of stemness core transcription factor genes ESRRB , SOX2 , KLF4 , NANOG , and OCT4 . ( F – I ), Effects of calcium on the induction of SFRP2 , PRL , IGF1 , and DPT . ( J – N ), Effects of cadmium on the induction of stemness core transcription factor genes ESRRB , SOX2 , KLF4 , NANOG , and OCT4 . ( O – R ), Effects of cadmium on the induction of SFRP2 , PRL , IGF1 , and DPT . Data are expressed as fold change (mean ± SEM); n = 3 to 7; statistical significance is defined as a p value of <0.05, p value is marked above if statistically significant.

Article Snippet: EsRRB Taqman probe , Thermo Fisher Scientific, Waltham, MA, USA , Hs01584024_m1.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Calcium and Cadmium Activate ESRRB to Mediate Cell Stemness and Pluripotency

doi: 10.3390/ijms27010231

Figure Lengend Snippet: Effects of calcium and cadmium on ESRRB-regulated genes in MDA-MB-453 cells. ( A – E ), Effects of calcium and cadmium on the induction of stemness core transcription factor genes ESRRB , SOX2 , KLF4 , NANOG , and OCT4 . ( F – H ), Effects of calcium and cadmium on the induction of ESR1 , IGF1 , and DPT ; data are expressed as fold change (mean ± SEM); n = 5 to 7. ( I ), Effects of extracellular calcium treatment on the concentration of intracellular calcium. Data are expressed as calcium concentration in nM (mean ± SEM); n = 3. Statistical significance is defined as a p value of <0.05, p value is marked above if statistically significant.

Article Snippet: EsRRB Taqman probe , Thermo Fisher Scientific, Waltham, MA, USA , Hs01584024_m1.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Calcium and Cadmium Activate ESRRB to Mediate Cell Stemness and Pluripotency

doi: 10.3390/ijms27010231

Figure Lengend Snippet: Effects of calcium and cadmium on ESRRB localization in MDA-MB-453 cells. ( A – D ), Immunofluorescence representative images of ESRRB in control ( A ), calcium ( B ), cadmium ( C ) and GSK4716 ( D ) treated cells. The second row of panels contains the zoomed-in images marked in yellow boxes in the top panels; red is ESRRB staining; blue is nuclear staining with DAPI; green is actin staining with phalloidin; bottom panels are merged IF pictures; the scale bar represents 25 μm.

Article Snippet: EsRRB Taqman probe , Thermo Fisher Scientific, Waltham, MA, USA , Hs01584024_m1.

Techniques: Immunofluorescence, Control, Staining

Journal: International Journal of Molecular Sciences

Article Title: Calcium and Cadmium Activate ESRRB to Mediate Cell Stemness and Pluripotency

doi: 10.3390/ijms27010231

Figure Lengend Snippet: Effects of calcium and cadmium on the recruitment of ESRRB and RNA polymerase 2 to enhancers of ESRRB-regulated genes in MDA-MB-453 cells. ( A – O ), Effect of calcium, cadmium, and GSK4716 on the binding of ESRRB to the enhancer of ESRRB ( A – C ), SOX2 ( D – F ), KLF4 upstream enhancer ( G – I ) and intron enhancer ( J – L ), and ESR1 ( M – O ). ( A , D , G , J , M ) illustrate the location of ERREs (estrogen-related receptor response elements) in the enhancer of target genes; the consensus sequences identical to classic ERRE (TNAAGGTCA) are highlighted in gray; the arrows indicate the transcription start site of each gene. ( B , E , H , K , N ) are the fold change in the binding of ESRRB to ERRE normalized to control (ChIP). Data are expressed as fold change in control in % input (mean ± SEM); n = 3. ( C , F , I , L , O ) are the fold change in the recruitment of Pol2 to ERRE to control (re-ChIP; first ESRRB pulldown then Pol2 pulldown). Data are expressed as fold change in control in % input (mean ± SEM); n = 2. Statistical significance is defined as a p value of <0.05, p value is marked above if statistically significant.

Article Snippet: EsRRB Taqman probe , Thermo Fisher Scientific, Waltham, MA, USA , Hs01584024_m1.

Techniques: Binding Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Calcium and Cadmium Activate ESRRB to Mediate Cell Stemness and Pluripotency

doi: 10.3390/ijms27010231

Figure Lengend Snippet: Identification of potential metal interaction sites on the LBD of ESRRB. ( A ), Chimeric GAL4-ESRRBsf-LBD construct. Created in BioRender. Shi, X. (2025) https://BioRender.com/a4hn9su (accessed on 16 October 2025). ( B ), Effect of cadmium and GSK4716 on WT LBD and GAL-4 plasmid. ( C ), Three-dimensional structure of ESRRB-LBD (6LN4); red dots represent H 2 O molecules. ( D ), Predicted interaction sites of calcium or cadmium on helix 4, 10,11 and 12; purple dots represent Ca 2+ or Cd 2+ at potential interaction sites. ( E ), CAT activity of GAL4-ESRRBsf-LBD mutants. ( F , I , L , N ) show the amino acid residues of predicted sites in 3D structure. ( G , H , J , K , M , O , P ), Effects of cadmium and GSK4716 on GAL-4-ESRRB-LBD mutants. Data are expressed as fold change (mean ± SEM); n ≥ 3; statistical significance is defined as a p value of <0.05, p value is marked above if statistically significant. LBD refers to ligand binding domain.

Article Snippet: EsRRB Taqman probe , Thermo Fisher Scientific, Waltham, MA, USA , Hs01584024_m1.

Techniques: Construct, Plasmid Preparation, Activity Assay, Ligand Binding Assay

Journal: Acta physiologica (Oxford, England)

Article Title: Fam163a knockdown and mitochondrial stress in the arcuate nucleus of hypothalamus reduce AgRP neuron activity and differentially regulate mitochondrial dynamics in mice.

doi: 10.1111/apha.70020

Figure Lengend Snippet: FIGURE 3 Fam163a knockdown and rotenone reduce the firing rate of ARCAgRP neurons. (A and D) Representative immunostaining against FAM163A. White arrows depict the co-expression of GFP and FAM163A (Scale bars: [A and C] 50 μm and [B and D] 20 μm). (E) GFP expression in AgRP neurons containing brain slice of AgRP-Cre mouse. (Representative image depicting Cell-attached loose-seal recording from an AgRP neuron; recording pipette is highlighted with red-dashed lines, Scale bar: 15 μm). (F and H) Representative loose- seal recording traces for each group. (G) Effect of Fam163a KD on firing rate of AgRP neurons (n = 3–4 mice for each group; Neg. Ctr. n = 70 neurons, Fam163a KD n = 63 neurons) (I) Effect of rotenone on firing rate of AgRP neurons (n = 3–4 mice for each group; DMSO n = 46 neurons, rotenoe n = 52 neurons) (Data are mean ± standard deviation. Statistical analysis: Mann–Whitney U test; *p = 0.017; **p = 0.0062).

Article Snippet: Each Agrp- ires- cre mouse also received an injection of AAV pCAG- FLEX- EGFP- WPRE (AddGene, 51502- AAV9) to label AgRP neurons for electrophysiological recordings.113,114

Techniques: Knockdown, Immunostaining, Expressing, Slice Preparation, Transferring, Standard Deviation, MANN-WHITNEY