Journal: Cellular & Molecular Biology Letters

Article Title: The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs

doi: 10.1186/s11658-026-00867-2

Figure Lengend Snippet: Enhanced cell growth of PDLSCs facilitated by GIPRA/MAPK/ERK axis. a Western blot assay showing the expression of Ki-67, PCNA, and CCND3 affected by GIPRA as well as inhibition of cAMP/PKA/CREB, MAPK/ERK, Wnt/β-catenin, or JAK/STAT3 signaling pathways. The interpretation of bubble colors in the line chart is illustrated in Fig. a. b CCK-8 assay indicating the cell growth enhanced by GIPRA, but suppressed by ERK Inh ( n = 5 in each group). The interpretation of line colors in the line chart is illustrated in Fig. b. c Venn diagram showing the intersection of differentially binding genes by p-CREB, p-ERK1/2, TCF4, and p-STAT3 (log FC > 1, P < 0.05) compared between GIPRA and NC group. d The bubble plot displays the top ten functions of cell division and cell cycle (GO biological process) associated with the 5994 (red color highlighted) specifically binding genes by p-ERK1/2 in c . e – g IGV showing the binding peaks of p-ERK1/2 on MKI67 ( e ), PCNA ( f ), and CCND3 genes ( g ). The differential binding peaks of p-ERK1/2 on these gene promoters are highlighted by blue boxes. h – k Line plot illustrates the distribution of ChIP-seq signal intensity of p-ERK1/2 ( h ), p-CREB ( i ), TCF4 ( j ), and p-STAT3 ( k ) across the transcription start sites (±3 kb) of differentially binding genes in PDLSCs treated with GIPRA as well as GIPRA plus ERK Inh. NC, normal PDLSCs as negative control; Inh., inhibitor

Article Snippet: The in vitro treatment conditions for the small-molecule compound were as follows: GLP1RA semaglutide acetate (10 nM, 24 h), GIPRA (Pro3) GIP (0.5 nM, 24 h), GLP-1R/GIPR agonist-1 (10 nM, 24 h) also termed as 2GRA in this study, BGM0504 (2 nM, 24 h, BrightGene Pharmaceutical Co., Ltd., China) [ ], CREB inhibitor 666-15 (1 μM, 2 h, HY-101120, MCE), Akt inhibitor MK-2206 (5 μM, 24 h, HY-108232, MCE), ERK1/2 inhibitor SCH772984 (0.5 μM, 24 h, HY-50846, MCE), β-catenin inhibitor IN-3 (5 μM, 24 h, HY-147007, MCE), STAT3-IN-14 (5 μM, 2 h, HY-N10472, MCE).

Techniques: Western Blot, Expressing, Inhibition, Protein-Protein interactions, CCK-8 Assay, Binding Assay, ChIP-sequencing, Negative Control

Journal: Journal of Animal Science and Biotechnology

Article Title: Dietary β-hydroxy-β-methyl butyrate supplementation improves intestinal health and growth performance in Tibetan sheep lambs via modulating small intestinal microbiota

doi: 10.1186/s40104-025-01345-z

Figure Lengend Snippet: Effects of HMB on the PLCβ/ERK signaling pathway in the ileum of Tibetan lambs. A – B Representative protein blots of PLCβ1 and ERK1/2 ( n = 3). C – D The proportion of Ki67-positive cells in the basal region of ileal crypts ( n = 6). HMB was supplemented at 0 mg/kg (CON group) and 715 mg/kg (M-HMB) of diet. ERK1/2: extracellular signal-related kinase 1/2, PLCβ1: phospholipase C beta 1. Data are represented as means ± SEM. * P < 0.05

Article Snippet: The primary antibodies used include PLCβ1 (AB_2838688, Affinity, USA), p-ERK1/2 (bs-3016R, Bioss, China), ERK1/2 (bs-2637R, Bioss, China), and GAPDH (60004-1-Ig, Proteintech, China).

Techniques:

Journal: Journal of Animal Science and Biotechnology

Article Title: Dietary β-hydroxy-β-methyl butyrate supplementation improves intestinal health and growth performance in Tibetan sheep lambs via modulating small intestinal microbiota

doi: 10.1186/s40104-025-01345-z

Figure Lengend Snippet: Effects of HMB on the PLCβ/ERK signaling pathway in the ileum of Tibetan lambs. A – B Representative protein blots of PLCβ1 and ERK1/2 ( n = 3). C – D The proportion of Ki67-positive cells in the basal region of ileal crypts ( n = 6). HMB was supplemented at 0 mg/kg (CON group) and 715 mg/kg (M-HMB) of diet. ERK1/2: extracellular signal-related kinase 1/2, PLCβ1: phospholipase C beta 1. Data are represented as means ± SEM. * P < 0.05

Article Snippet: The primary antibodies used include PLCβ1 (AB_2838688, Affinity, USA), p-ERK1/2 (bs-3016R, Bioss, China), ERK1/2 (bs-2637R, Bioss, China), and GAPDH (60004-1-Ig, Proteintech, China).

Techniques: