erk1 Search Results


erk1 2  (NSJ Bioreagents)
91
NSJ Bioreagents erk1 2
Erk1 2, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p erk  (Huabio Inc)
86
Huabio Inc p erk
P Erk, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit monoclonal  (Abcam)
99
Abcam rabbit monoclonal
Rabbit Monoclonal, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho erk1  (R&D Systems)
93
R&D Systems phospho erk1
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alphalisa surefire ultra perk 1 2  (Revvity)
90
Revvity alphalisa surefire ultra perk 1 2
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dyc1018b  (R&D Systems)
94
R&D Systems dyc1018b
Dyc1018b, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti tnf α mab 1825 12  (R&D Systems)
90
R&D Systems anti tnf α mab 1825 12
Anti Tnf α Mab 1825 12, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary anti erk1/2  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology primary anti erk1/2
Primary Anti Erk1/2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti erk1 erk2  (R&D Systems)
93
R&D Systems anti erk1 erk2
Anti Erk1 Erk2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p erk1 erk2  (R&D Systems)
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R&D Systems p erk1 erk2
P Erk1 Erk2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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erk1  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology erk1
Figure 7 70Z-Cbl expression does not constitutively activate MAP kinases <t>ERK1</t> and ERK2. (a) Analysis of MAP kinase activation by electrophoretic mobility shift in SDS-polyacrylamide gels. NIH3T3 cells expressing the human EGFR and HA-tagged c-Cbl, 70Z-Cbl or v-Cbl constructs were grown in media containing 0.5% FCS for 24 h. Cells were left quiescent (EGF7) or were stimulated with 1 ng/ml of EGF for 2 min at 378C (EGF+) before lysis and immunoblotting of cytoplasmic lysates with ERK antibodies. The activation of the two forms of MAP kinase is indicated by the phosphorylation-mediated shift in mobility of p42 (ERK2) and p44 (ERK1). (b) Analysis of MEK activation by immunoblotting with a phospho-speci®c MEK antibody. (c) Analysis of MEK protein levels by immunoblotting with a polyclonal MEK antibody
Erk1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ab 144p rabbit anti p erk  (Proteintech)
96
Proteintech ab 144p rabbit anti p erk
Figure 7 70Z-Cbl expression does not constitutively activate MAP kinases <t>ERK1</t> and ERK2. (a) Analysis of MAP kinase activation by electrophoretic mobility shift in SDS-polyacrylamide gels. NIH3T3 cells expressing the human EGFR and HA-tagged c-Cbl, 70Z-Cbl or v-Cbl constructs were grown in media containing 0.5% FCS for 24 h. Cells were left quiescent (EGF7) or were stimulated with 1 ng/ml of EGF for 2 min at 378C (EGF+) before lysis and immunoblotting of cytoplasmic lysates with ERK antibodies. The activation of the two forms of MAP kinase is indicated by the phosphorylation-mediated shift in mobility of p42 (ERK2) and p44 (ERK1). (b) Analysis of MEK activation by immunoblotting with a phospho-speci®c MEK antibody. (c) Analysis of MEK protein levels by immunoblotting with a polyclonal MEK antibody
Ab 144p Rabbit Anti P Erk, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 7 70Z-Cbl expression does not constitutively activate MAP kinases ERK1 and ERK2. (a) Analysis of MAP kinase activation by electrophoretic mobility shift in SDS-polyacrylamide gels. NIH3T3 cells expressing the human EGFR and HA-tagged c-Cbl, 70Z-Cbl or v-Cbl constructs were grown in media containing 0.5% FCS for 24 h. Cells were left quiescent (EGF7) or were stimulated with 1 ng/ml of EGF for 2 min at 378C (EGF+) before lysis and immunoblotting of cytoplasmic lysates with ERK antibodies. The activation of the two forms of MAP kinase is indicated by the phosphorylation-mediated shift in mobility of p42 (ERK2) and p44 (ERK1). (b) Analysis of MEK activation by immunoblotting with a phospho-speci®c MEK antibody. (c) Analysis of MEK protein levels by immunoblotting with a polyclonal MEK antibody

Journal: Oncogene

Article Title: Tyrosine kinase activity of the EGF receptor is enhanced by the expression of oncogenic 70Z-Cbl.

doi: 10.1038/sj.onc.1201468

Figure Lengend Snippet: Figure 7 70Z-Cbl expression does not constitutively activate MAP kinases ERK1 and ERK2. (a) Analysis of MAP kinase activation by electrophoretic mobility shift in SDS-polyacrylamide gels. NIH3T3 cells expressing the human EGFR and HA-tagged c-Cbl, 70Z-Cbl or v-Cbl constructs were grown in media containing 0.5% FCS for 24 h. Cells were left quiescent (EGF7) or were stimulated with 1 ng/ml of EGF for 2 min at 378C (EGF+) before lysis and immunoblotting of cytoplasmic lysates with ERK antibodies. The activation of the two forms of MAP kinase is indicated by the phosphorylation-mediated shift in mobility of p42 (ERK2) and p44 (ERK1). (b) Analysis of MEK activation by immunoblotting with a phospho-speci®c MEK antibody. (c) Analysis of MEK protein levels by immunoblotting with a polyclonal MEK antibody

Article Snippet: The proteins were transferred to a nitrocellulose membrane and immunoblotted with a rabbit antiMAPK antibody which recognizes ERK1 and ERK2 (Santa Cruz, SC-94).

Techniques: Expressing, Activation Assay, Electrophoretic Mobility Shift Assay, Construct, Lysis, Western Blot, Phospho-proteomics