Journal: bioRxiv
Article Title: A Novel Therapeutic Approach: Notch Inhibition Enhances Radiotherapy and Checkpoint Blockade Therapy via Reprogramming of the Tumor Microenvironment
doi: 10.64898/2025.12.30.696685
Figure Lengend Snippet: (A) UMAPs showing M1-like and M2-like macrophage states across treatments in 9464D tumor scRNA-seq. (B) Proportion of M1- and M2-like macrophages by treatment group (C) Expression of classical M1 (Il1b, Tnf) and M2 (Mrc1, Mgl2) markers. (D) M-MDSC module scores increase following RT + aPD-1 + GSI (E) Changes in the proportion of M1 and M2 macrophages subpopulation and Myeloid-derived suppressor cells (MDSC) subpopulations by flow cytometry in 9464D tumor (F) Changes in the proportion of M1 and M2 macrophages subpopulation and MDSC subpopulations by flow cytometry in EO771 tumor. Bars represent the mean. Each dot represents one animal. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 using one-way ANOVA followed by Tukey post-hoc comparisons.
Article Snippet: EO771 cells (purchased from ATCC) and the 9464D cells (derived from a TH-MYCN transgenic neuroblastoma mouse, obtained as a gift from Dr.
Techniques: Expressing, Derivative Assay, Flow Cytometry