Journal: Chemmedchem
Article Title: Cytotoxicity of Atropisomeric [1,1′‐Binaphthalene]‐2,2′‐Diamines (BINAM) and Analogs in Human Cancer Cells: Enantioselectivity, Structure–Activity Relationships, and Mechanism
doi: 10.1002/cmdc.202500426
Figure Lengend Snippet: Impact of 1( R ) and 1( S ) on the motor proteins KIF1C, Eg5, and the microtubule destabilizing protein stathmin. Nuclear DNA is displayed in blue in all images. The scale bar (10 µm) is valid for the respective row of images. A) 1( R ) has no effects on the distribution of KIF1C (green). B) Eg5 (green) is diffusely distributed in mitotic cells treated with 1( R ) . C) 1( R ) treated cells do not show an induction of stathmin (green).
Article Snippet: Antibodies used were as follows: Alexa Fluor 488 conjugate (#4412, CST, 1:500), Alexa Fluor 594 conjugate (#8890, CST, 1:500), alpha‐tubulin (#11224‐1‐AP, Proteintech, 1:100), acetyl alpha‐tubulin (#5335, CST, 1:100), centrin (#ZMS1054, Sigma–Aldrich, 1:100), detyrosinated alpha‐tubulin (#abx375026, Abbexa, 1:100), EB1 (#17717‐1‐AP, Proteintech, 1:200), Eg5 (#23333‐1‐AP, Proteintech, 1:200), gamma‐tubulin (#CL594‐66320, Proteintech, 1:100), KIF1C (#12760‐1‐AP, Proteintech, 1:100), stathmin 1 (#11157‐1‐AP, Proteintech, 1:100).
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