Journal: Microbial Biotechnology
Article Title: A Modified Method Incorporating Multiplex PCR Reveals Fusobacterium Prevalence in Southern Chinese Population and Its Correlations in Cancers
doi: 10.1111/1751-7915.70292
Figure Lengend Snippet: The prevalence of Fusobacterium in faeces of cancer patients with correlations to host conditions. (A) The prevalence of Fusobacterium in cancer patients ( n = 254) and healthy controls ( n = 82); (B–E) The prevalence of Fusobacterium species and subspecies in cancer patients ( n = 254) and healthy controls ( n = 82); (F–N) The relative abundance of FN (F), FNN (G), FNA (H), FNV (I), FNP (J), FM (K), FU (L), FV (M) and FNE (N) in faecal samples of cancer patients ( n = 254) and healthy controls ( n = 82). Mann–Whitney test. (O) The correlations between Fusobacterium and host parameters in cancer patients ( n = 254). The correlation analysis is spearman analysis. A higher absolute value of the correlation coefficient signifies a stronger correlation, with red denoting a positive correlation and blue indicating a negative correlation. The size of the nodes and the depth of their colour represent the strength of the correlation, where larger nodes and darker hues correspond to stronger correlations. An asterisk (*) denotes the p ‐value. (P) The correlations between Fusobacterium and host parameters in healthy controls ( n = 82). The correlation analysis is spearman analysis. A higher absolute value of the correlation coefficient signifies a stronger correlation, with red denoting a positive correlation and blue indicating a negative correlation. The size of the nodes and the depth of their colour represent the strength of the correlation, where larger nodes and darker hues correspond to stronger correlations. An asterisk (*) denotes the p ‐value. (Q) The receiver operating characteristic (ROC) curve for predicting cancers. AUC, the area under the ROC curve. The 95% confidence intervals are shown in brackets. (R) The prevalence of Fusobacterium in untreated ( n = 64), treated (190) and control ( n = 82) group. (S, T) The prevalence of Fusobacterium species and subspecies in untreated ( n = 64), treated (190) and control ( n = 82) group. (U–AC) The relative abundance of FN (U), FNN (V), FNA (W), FNV (X), FNP (Y), FM (Z), FU (AA), FV (AB) and FNE (AC) in faecal samples of untreated ( n = 64) and treated ( n = 190) cancer patients and control group ( n = 82). Kruskal–Wallis H test followed by Dunn's test. FM, Fusobacterium mortiferum ; FN, Fusobacterium nucleatum ; FNA, Fusobacterium nucleatum subsp. animalis ; FNE, Fusobacterium necrophorum .; FNN, Fusobacterium nucleatum subsp. nucleatum ; FNP, Fusobacterium nucleatum subsp. polymorphum ; FNV, Fusobacterium nucleatum subsp. vincentii ; FU, Fusobacterium ulcerans ; FV, Fusobacterium varium .
Article Snippet: F. nucleatum subsp. nucleatum (ATCC 25586), F. nucleatum subsp. nucleatum (ATCC 23726), F. nucleatum subsp. polymorphum (ATCC 10953), F. nucleatum subsp. vincentii (ATCC 49256), F. nucleatum subsp. animals (ATCC 51191), F. necrophorum ATCC (25286), Lactobacillus iners (ATCC 55195), Bacteroides thetaiotaomicron (ATCC 29148) were from American Type Culture Collection (ATCC, USA).
Techniques: MANN-WHITNEY, Control