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rhodococcus opacus pd630  (DSMZ)


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    Structured Review

    DSMZ rhodococcus opacus pd630
    Testing Rhodococcus strains for growth in the presence of DMT and degradation of DMT. a) and b) Growth curves of <t>PD630</t> and RPET in minimal media supplemented with 1 g/L glucose and varying concentrations of DMT (0, 5, 10, 15 and 20 mg/L), respectively; c) and d) DMT degradation percentages for PD630 and RPET, respectively. The inocula of both strains were pre-cultured using glucose-containing minimal media. All experiments were conducted in triplicate, and error bars indicate standard deviations.
    Rhodococcus Opacus Pd630, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dsm/Rhodococcus+opacus/pmc12856551-212-0-6
    Average 94 stars, based on 72 article reviews
    rhodococcus opacus pd630 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Elucidating biodegradation of dimethyl terephthalate by two Rhodococcus strains for its valorization applications"

    Article Title: Elucidating biodegradation of dimethyl terephthalate by two Rhodococcus strains for its valorization applications

    Journal: Metabolic Engineering Communications

    doi: 10.1016/j.mec.2026.e00271

    Testing Rhodococcus strains for growth in the presence of DMT and degradation of DMT. a) and b) Growth curves of PD630 and RPET in minimal media supplemented with 1 g/L glucose and varying concentrations of DMT (0, 5, 10, 15 and 20 mg/L), respectively; c) and d) DMT degradation percentages for PD630 and RPET, respectively. The inocula of both strains were pre-cultured using glucose-containing minimal media. All experiments were conducted in triplicate, and error bars indicate standard deviations.
    Figure Legend Snippet: Testing Rhodococcus strains for growth in the presence of DMT and degradation of DMT. a) and b) Growth curves of PD630 and RPET in minimal media supplemented with 1 g/L glucose and varying concentrations of DMT (0, 5, 10, 15 and 20 mg/L), respectively; c) and d) DMT degradation percentages for PD630 and RPET, respectively. The inocula of both strains were pre-cultured using glucose-containing minimal media. All experiments were conducted in triplicate, and error bars indicate standard deviations.

    Techniques Used: Cell Culture

    Resting cell assay of PD630 and RPET in the presence of DMT. a) and b) DMT concentration profiles of PD630 and RPET, respectively; c) and d) MMT concentration profiles of PD630 and RPET, respectively; e) and f) MMT concentration profiles of PD630 and RPET over an extended period, respectively. The control groups were pre-grown in minimal media with 1 g/L glucose, while the induced groups were pre-grown in minimal media with 1 g/L glucose and 20 mg/L DMT to promote the expression of genes related to DMT degradation. Then, cells were harvested at the mid-exponential phase and resuspended in 1X phosphate-buffered saline (pH 7.0) containing 20 mg/L DMT at an equal initial OD 600 value. For a), b), c), and d), the OD 600 profiles after resuspension are shown in . For e) and f), the DMT concentration profiles and OD 600 profiles are shown in . All experiments were performed in triplicate, and error bars represent standard deviations.
    Figure Legend Snippet: Resting cell assay of PD630 and RPET in the presence of DMT. a) and b) DMT concentration profiles of PD630 and RPET, respectively; c) and d) MMT concentration profiles of PD630 and RPET, respectively; e) and f) MMT concentration profiles of PD630 and RPET over an extended period, respectively. The control groups were pre-grown in minimal media with 1 g/L glucose, while the induced groups were pre-grown in minimal media with 1 g/L glucose and 20 mg/L DMT to promote the expression of genes related to DMT degradation. Then, cells were harvested at the mid-exponential phase and resuspended in 1X phosphate-buffered saline (pH 7.0) containing 20 mg/L DMT at an equal initial OD 600 value. For a), b), c), and d), the OD 600 profiles after resuspension are shown in . For e) and f), the DMT concentration profiles and OD 600 profiles are shown in . All experiments were performed in triplicate, and error bars represent standard deviations.

    Techniques Used: Concentration Assay, Control, Expressing, Saline

    Transcriptomic analyses of PD630 and RPET. a) Results of PD630; b) Results of RPET. The volcano plots illustrate differential gene expression in response to DMT, determined by transcriptomic analysis. Red and blue dots represent genes with statistically significant up- and down-regulation, respectively (|log 2 (fold change)| > 1 and adjusted p -value <0.05); black dots indicate genes with statistically insignificant changes. The horizontal dashed line represents adjusted p -value = 0.05. Transcriptomic data were generated by Genewiz® using RNA-seq service. The control groups were cultured in minimal media with glucose as the sole carbon source, while the experimental groups were cultured in minimal media with glucose and DMT (see Materials and Methods for details). Data analysis was conducted in R software using DESeq2 and clusterProfiler.
    Figure Legend Snippet: Transcriptomic analyses of PD630 and RPET. a) Results of PD630; b) Results of RPET. The volcano plots illustrate differential gene expression in response to DMT, determined by transcriptomic analysis. Red and blue dots represent genes with statistically significant up- and down-regulation, respectively (|log 2 (fold change)| > 1 and adjusted p -value <0.05); black dots indicate genes with statistically insignificant changes. The horizontal dashed line represents adjusted p -value = 0.05. Transcriptomic data were generated by Genewiz® using RNA-seq service. The control groups were cultured in minimal media with glucose as the sole carbon source, while the experimental groups were cultured in minimal media with glucose and DMT (see Materials and Methods for details). Data analysis was conducted in R software using DESeq2 and clusterProfiler.

    Techniques Used: Gene Expression, Generated, RNA Sequencing, Control, Cell Culture, Software

    DMT and MMT degradation by PD630 and RPET knockout strains. a) DMT and MMT concentration profiles of PD630 knockout strains with DMT as substrate; b) DMT and MMT concentration profiles of RPET knockout strains with DMT Feed or MMT Feed. The strains were cultured in minimal media supplemented with 1 g/L glucose and 20 mg/L DMT or MMT, and samples were collected 8 h after inoculation. c) RPET ΔRS21885 cultured in two conditions: Glu only (1 g/L glucose alone) and Glu + DMT (1 g/L glucose and 1 g/L DMT); d) DMT and MMT concentration profiles of RPET ΔRS21885 under Glu + DMT condition. The inocula for all were pre-grown in glucose-containing minimal media. In a) and b), dashed lines indicate the initial substrate concentrations; ∗∗ indicates p < 0.01, and ∗∗∗ indicates p < 0.001. In d), the dashed line indicates the theoretical concentration of 1 g/L DMT if completely dissolved. All experiments were performed in triplicate, and error bars represent standard deviations. Details related to the knockout genes are provided in .
    Figure Legend Snippet: DMT and MMT degradation by PD630 and RPET knockout strains. a) DMT and MMT concentration profiles of PD630 knockout strains with DMT as substrate; b) DMT and MMT concentration profiles of RPET knockout strains with DMT Feed or MMT Feed. The strains were cultured in minimal media supplemented with 1 g/L glucose and 20 mg/L DMT or MMT, and samples were collected 8 h after inoculation. c) RPET ΔRS21885 cultured in two conditions: Glu only (1 g/L glucose alone) and Glu + DMT (1 g/L glucose and 1 g/L DMT); d) DMT and MMT concentration profiles of RPET ΔRS21885 under Glu + DMT condition. The inocula for all were pre-grown in glucose-containing minimal media. In a) and b), dashed lines indicate the initial substrate concentrations; ∗∗ indicates p < 0.01, and ∗∗∗ indicates p < 0.001. In d), the dashed line indicates the theoretical concentration of 1 g/L DMT if completely dissolved. All experiments were performed in triplicate, and error bars represent standard deviations. Details related to the knockout genes are provided in .

    Techniques Used: Knock-Out, Concentration Assay, Cell Culture

    Related Articles

    Derivative Assay:

    Article Title: Corallopyronin A exhibits potent activity against staphylococci including MRSA and isolates from prosthetic infections.
    Article Snippet: .. 7 S. aureus ATCC 29213, DSM 2569 0.5 DSMZ - wound ST5 8 S. aureus HG001 0.25 Herbert et al. 2010 [37] - lab strain ST8 9 S. aureus DSM 28763 ECC5055 0.25 DSMZ - wound ST8 10 S. aureus Newman ATCC25904 0.25 ATCC - lab strain ST254 11 S. aureus Wood 46 DSM 20491 0.25 DSMZ - lab strain ST97 12 S. aureus SG511-Berlin 0.25 Dietrich et al. 2021[38] - lab strain ST30 13 S. aureus SA113, NCTC 8325 0.25 NCTC - lab strain ST8 14 S. aureus DSM 20231 0.25 DSMZ - type strain, pleural fluid ST8 15 S. aureus 15981 0.25 Valle et al. 2003 [39] - tissue ST50 16 S. aureus I10 0.25 von Eiff et al. 1997, derived from S. aureus NCTC 8325–4 [29] Ery Stable SCV, lab strain ST8 At hourly intervals from 1 to 5 h and again after 24 h, 100 μL samples were collected from both the control and the CorA-treated cultures. ..

    Control:

    Article Title: Corallopyronin A exhibits potent activity against staphylococci including MRSA and isolates from prosthetic infections.
    Article Snippet: .. 7 S. aureus ATCC 29213, DSM 2569 0.5 DSMZ - wound ST5 8 S. aureus HG001 0.25 Herbert et al. 2010 [37] - lab strain ST8 9 S. aureus DSM 28763 ECC5055 0.25 DSMZ - wound ST8 10 S. aureus Newman ATCC25904 0.25 ATCC - lab strain ST254 11 S. aureus Wood 46 DSM 20491 0.25 DSMZ - lab strain ST97 12 S. aureus SG511-Berlin 0.25 Dietrich et al. 2021[38] - lab strain ST30 13 S. aureus SA113, NCTC 8325 0.25 NCTC - lab strain ST8 14 S. aureus DSM 20231 0.25 DSMZ - type strain, pleural fluid ST8 15 S. aureus 15981 0.25 Valle et al. 2003 [39] - tissue ST50 16 S. aureus I10 0.25 von Eiff et al. 1997, derived from S. aureus NCTC 8325–4 [29] Ery Stable SCV, lab strain ST8 At hourly intervals from 1 to 5 h and again after 24 h, 100 μL samples were collected from both the control and the CorA-treated cultures. ..

    Food & Beverages:

    Article Title: In vitro digestive system simulation and anticancer activity of soymilk fermented by probiotics and synbiotics immobilised on agro-industrial residues
    Article Snippet: .. These strains were Lactiplantibacillus plantarum ATCC 14,917, Lacticasibacillus casei DS 20,011, Lactobacillus acidophilus ATCC 20,552, Streptococcus thermophilus DSM 20,259, and Bifidobacterium longum B 41,409) which collected from the Food Technology Research Institute, Agricultural Research Center in Giza, Egypt, while Lactiplantibacillus plantarum DSA 20,174, Limosilactobacillus reuteri NRRL B-14171, Lactobacillus delbrueckii subsp. bulgaricus DSMZ 20,080, Lacticaseibacillus rhamnosus NRRL B-442, and Bifidobacterium bifidum NRRL B-41410 were collected from the Dairy Department, National Research Center in Giza, Egypt. ..

    Plasmid Preparation:

    Article Title: Algal exudates promote conjugation in marine Roseobacters
    Article Snippet: .. Phaeobacter inhibens DSM 17395 262 kb plasmid 138,002 Dr. Jӧrn Petersen from the Leibniz Institute, DSMZ, Germany. ..



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    Image Search Results


    Testing Rhodococcus strains for growth in the presence of DMT and degradation of DMT. a) and b) Growth curves of PD630 and RPET in minimal media supplemented with 1 g/L glucose and varying concentrations of DMT (0, 5, 10, 15 and 20 mg/L), respectively; c) and d) DMT degradation percentages for PD630 and RPET, respectively. The inocula of both strains were pre-cultured using glucose-containing minimal media. All experiments were conducted in triplicate, and error bars indicate standard deviations.

    Journal: Metabolic Engineering Communications

    Article Title: Elucidating biodegradation of dimethyl terephthalate by two Rhodococcus strains for its valorization applications

    doi: 10.1016/j.mec.2026.e00271

    Figure Lengend Snippet: Testing Rhodococcus strains for growth in the presence of DMT and degradation of DMT. a) and b) Growth curves of PD630 and RPET in minimal media supplemented with 1 g/L glucose and varying concentrations of DMT (0, 5, 10, 15 and 20 mg/L), respectively; c) and d) DMT degradation percentages for PD630 and RPET, respectively. The inocula of both strains were pre-cultured using glucose-containing minimal media. All experiments were conducted in triplicate, and error bars indicate standard deviations.

    Article Snippet: Rhodococcus opacus PD630 was obtained from DSMZ (identifier: DSMZ 44193).

    Techniques: Cell Culture

    Resting cell assay of PD630 and RPET in the presence of DMT. a) and b) DMT concentration profiles of PD630 and RPET, respectively; c) and d) MMT concentration profiles of PD630 and RPET, respectively; e) and f) MMT concentration profiles of PD630 and RPET over an extended period, respectively. The control groups were pre-grown in minimal media with 1 g/L glucose, while the induced groups were pre-grown in minimal media with 1 g/L glucose and 20 mg/L DMT to promote the expression of genes related to DMT degradation. Then, cells were harvested at the mid-exponential phase and resuspended in 1X phosphate-buffered saline (pH 7.0) containing 20 mg/L DMT at an equal initial OD 600 value. For a), b), c), and d), the OD 600 profiles after resuspension are shown in . For e) and f), the DMT concentration profiles and OD 600 profiles are shown in . All experiments were performed in triplicate, and error bars represent standard deviations.

    Journal: Metabolic Engineering Communications

    Article Title: Elucidating biodegradation of dimethyl terephthalate by two Rhodococcus strains for its valorization applications

    doi: 10.1016/j.mec.2026.e00271

    Figure Lengend Snippet: Resting cell assay of PD630 and RPET in the presence of DMT. a) and b) DMT concentration profiles of PD630 and RPET, respectively; c) and d) MMT concentration profiles of PD630 and RPET, respectively; e) and f) MMT concentration profiles of PD630 and RPET over an extended period, respectively. The control groups were pre-grown in minimal media with 1 g/L glucose, while the induced groups were pre-grown in minimal media with 1 g/L glucose and 20 mg/L DMT to promote the expression of genes related to DMT degradation. Then, cells were harvested at the mid-exponential phase and resuspended in 1X phosphate-buffered saline (pH 7.0) containing 20 mg/L DMT at an equal initial OD 600 value. For a), b), c), and d), the OD 600 profiles after resuspension are shown in . For e) and f), the DMT concentration profiles and OD 600 profiles are shown in . All experiments were performed in triplicate, and error bars represent standard deviations.

    Article Snippet: Rhodococcus opacus PD630 was obtained from DSMZ (identifier: DSMZ 44193).

    Techniques: Concentration Assay, Control, Expressing, Saline

    Transcriptomic analyses of PD630 and RPET. a) Results of PD630; b) Results of RPET. The volcano plots illustrate differential gene expression in response to DMT, determined by transcriptomic analysis. Red and blue dots represent genes with statistically significant up- and down-regulation, respectively (|log 2 (fold change)| > 1 and adjusted p -value <0.05); black dots indicate genes with statistically insignificant changes. The horizontal dashed line represents adjusted p -value = 0.05. Transcriptomic data were generated by Genewiz® using RNA-seq service. The control groups were cultured in minimal media with glucose as the sole carbon source, while the experimental groups were cultured in minimal media with glucose and DMT (see Materials and Methods for details). Data analysis was conducted in R software using DESeq2 and clusterProfiler.

    Journal: Metabolic Engineering Communications

    Article Title: Elucidating biodegradation of dimethyl terephthalate by two Rhodococcus strains for its valorization applications

    doi: 10.1016/j.mec.2026.e00271

    Figure Lengend Snippet: Transcriptomic analyses of PD630 and RPET. a) Results of PD630; b) Results of RPET. The volcano plots illustrate differential gene expression in response to DMT, determined by transcriptomic analysis. Red and blue dots represent genes with statistically significant up- and down-regulation, respectively (|log 2 (fold change)| > 1 and adjusted p -value <0.05); black dots indicate genes with statistically insignificant changes. The horizontal dashed line represents adjusted p -value = 0.05. Transcriptomic data were generated by Genewiz® using RNA-seq service. The control groups were cultured in minimal media with glucose as the sole carbon source, while the experimental groups were cultured in minimal media with glucose and DMT (see Materials and Methods for details). Data analysis was conducted in R software using DESeq2 and clusterProfiler.

    Article Snippet: Rhodococcus opacus PD630 was obtained from DSMZ (identifier: DSMZ 44193).

    Techniques: Gene Expression, Generated, RNA Sequencing, Control, Cell Culture, Software

    DMT and MMT degradation by PD630 and RPET knockout strains. a) DMT and MMT concentration profiles of PD630 knockout strains with DMT as substrate; b) DMT and MMT concentration profiles of RPET knockout strains with DMT Feed or MMT Feed. The strains were cultured in minimal media supplemented with 1 g/L glucose and 20 mg/L DMT or MMT, and samples were collected 8 h after inoculation. c) RPET ΔRS21885 cultured in two conditions: Glu only (1 g/L glucose alone) and Glu + DMT (1 g/L glucose and 1 g/L DMT); d) DMT and MMT concentration profiles of RPET ΔRS21885 under Glu + DMT condition. The inocula for all were pre-grown in glucose-containing minimal media. In a) and b), dashed lines indicate the initial substrate concentrations; ∗∗ indicates p < 0.01, and ∗∗∗ indicates p < 0.001. In d), the dashed line indicates the theoretical concentration of 1 g/L DMT if completely dissolved. All experiments were performed in triplicate, and error bars represent standard deviations. Details related to the knockout genes are provided in .

    Journal: Metabolic Engineering Communications

    Article Title: Elucidating biodegradation of dimethyl terephthalate by two Rhodococcus strains for its valorization applications

    doi: 10.1016/j.mec.2026.e00271

    Figure Lengend Snippet: DMT and MMT degradation by PD630 and RPET knockout strains. a) DMT and MMT concentration profiles of PD630 knockout strains with DMT as substrate; b) DMT and MMT concentration profiles of RPET knockout strains with DMT Feed or MMT Feed. The strains were cultured in minimal media supplemented with 1 g/L glucose and 20 mg/L DMT or MMT, and samples were collected 8 h after inoculation. c) RPET ΔRS21885 cultured in two conditions: Glu only (1 g/L glucose alone) and Glu + DMT (1 g/L glucose and 1 g/L DMT); d) DMT and MMT concentration profiles of RPET ΔRS21885 under Glu + DMT condition. The inocula for all were pre-grown in glucose-containing minimal media. In a) and b), dashed lines indicate the initial substrate concentrations; ∗∗ indicates p < 0.01, and ∗∗∗ indicates p < 0.001. In d), the dashed line indicates the theoretical concentration of 1 g/L DMT if completely dissolved. All experiments were performed in triplicate, and error bars represent standard deviations. Details related to the knockout genes are provided in .

    Article Snippet: Rhodococcus opacus PD630 was obtained from DSMZ (identifier: DSMZ 44193).

    Techniques: Knock-Out, Concentration Assay, Cell Culture