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Structured Review

Proteintech drp1
Drp1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/drp1/DRP1+(C-terminal)+Antibody/10__3390_slash_antiox15101329-41-18-50
Average 96 stars, based on 339 article reviews
drp1 - by Bioz Stars, 2026-11
96/100 stars

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Related Articles

Saline:

Article Title: Adipose-derived mesenchymal stem cell exosomes enhance diabetic wound healing via the amelioration of fibroblast senescence through the SMARCAL1-Drp1 signaling pathway.
Article Snippet: Delayed healing of diabetic wounds (DW) represents a significant complication among diabetic patients, for which current therapeutic approaches remain suboptimal.. Accumulating evidence indicates that fibroblast senescence plays a critical role in the impaired healing of diabetic wounds.. Abnormal mitochondrial morphology has long been associated with cellular senescence and age-related pathologies, suggesting that mitochondrial dynamics are compromised during senescence.

Incubation:

Article Title: Adipose-derived mesenchymal stem cell exosomes enhance diabetic wound healing via the amelioration of fibroblast senescence through the SMARCAL1-Drp1 signaling pathway.
Article Snippet: Delayed healing of diabetic wounds (DW) represents a significant complication among diabetic patients, for which current therapeutic approaches remain suboptimal.. Accumulating evidence indicates that fibroblast senescence plays a critical role in the impaired healing of diabetic wounds.. Abnormal mitochondrial morphology has long been associated with cellular senescence and age-related pathologies, suggesting that mitochondrial dynamics are compromised during senescence.

Article Title: TRPV4 Deficiency Shifts Mitochondrial Dynamics Toward a Fragmented Morphology in Primary Microglia
Article Snippet: Following protein separation on 12% SDS-PAGE gels, samples were transferred to PVDF membranes (VWR, Radnor, PA, USA), which were blocked in 2% BSA (Sigma-Aldrich) for one hour at RT. .. Primary antibodies—Mfn1 (13798- 1-AP, 1:1000, Proteintech), pDrp1 (PA5-64821, 1:500, InvitrogenTM), total Drp1 (12957-1-AP, 1:1000, Proteintech), β-actin (sc-47778, 1:2000, Santa Cruz, Dallas, TX, USA), GAPDH (sc-137179, 1:2000, Santa Cruz)—were incubated overnight at 4 ◦C. .. Subsequently, the HRP-conjugated anti-rabbit (1:2000, Biotium, Fremont, CA, USA) secondary antibody was incubated for one hour at RT.

Article Title: CSRP3 promotes skeletal muscle remodeling toward aerobic metabolism and enhances exercise endurance through increasing LDHD activity.
Article Snippet: Exercise performance and skeletal muscle homeostasis are influenced by myofiber type composition.. Cysteine and glycine-rich protein 3 (CSRP3) is highly expressed in the oxidative fiber-rich mammalian soleus muscle.. However, the mechanistic basis of CSRP3's involvement in skeletal muscle development and myofiber type specification remains unclear.

Article Title: TRPV4 Deficiency Shifts Mitochondrial Dynamics Toward a Fragmented Morphology in Primary Microglia
Article Snippet: Following protein separation on 12% SDS-PAGE gels, samples were transferred to PVDF membranes (VWR, Radnor, PA, USA), which were blocked in 2% BSA (Sigma-Aldrich) for one hour at RT. .. Primary antibodies—Mfn1 (13798-1-AP, 1:1000, Proteintech), pDrp1 (PA5-64821, 1:500, Invitrogen TM ), total Drp1 (12957-1-AP, 1:1000, Proteintech), β-actin (sc-47778, 1:2000, Santa Cruz, Dallas, TX, USA), GAPDH (sc-137179, 1:2000, Santa Cruz)—were incubated overnight at 4 °C. .. Subsequently, the HRP-conjugated anti-rabbit (1:2000, Biotium, Fremont, CA, USA) secondary antibody was incubated for one hour at RT.

Aqueous Normal-phase Chromatography:

Article Title: Cryptotanshinone Alleviates Cardiac Hypertrophy Through SIRT3-Mediated Epigenetic Regulation and Mitochondrial Dynamics
Article Snippet: Phalloidin-iFluor 488 Reagent (ab176753) was obtained from Abcam (Waltham, MA, USA). .. Antibodies against ANP (Cat. No. 27426-1-AP), BNP (Cat. No. 28214-1-AP), SIRT3 (Cat. No. 10099-1-AP), H3K9ac (Cat. No. 39137), DRP1 (Cat. No. 12957-1-AP), MFN2 (Cat. No. 12186-1-AP), Bax (Cat. No. 50599-2-Ig), Bcl-2 (Cat. 68103-1-Ig), caspase-3 (Cat. No. 19677-1- AP), GAPDH (Cat. No. 60004-1-Ig), and β-actin (Cat. No. 66009-1-Ig) were obtained from Proteintech (Wuhan, China). .. 3-TYP (Cat. No. S8628) was supplied by Selleck Chemicals (Houston, TX, USA).

Cell-Signaling:

Article Title: GPR65 protects adipose-derived stem cell therapy against acidosis in hindlimb ischemia
Article Snippet: .. The primary antibodies are listed as follow: phospho-Akt (Ser473, 1:500, Signalway Antibody, Maryland, USA), Akt (1:1000, Proteintech, Rosemont, USA), phospho-p42/44 MAPK (1:1000, p-ERK1/2, Cell Signaling Technology, Danvers, USA), p42/44 MAPK (1:1000, ERK1/2, Cell Signaling Technology, Danvers, USA), GPR65 (1:500, Immunoway, California, USA), MFN1(1:2000, Proteintech, Rosemont, USA), MFN2 (1:2000, Abcam, Cambridge, UK), Drp1(1:2000, Proteintech, Rosemont, USA), OPA1(1:5000, Proteintech, Rosemont, USA), VEGF-A (1:500, Proteintech, Rosemont, USA), GAPDH (1:1000, Cell Signaling Technology, Danvers, USA) and β-actin (1:5000, Proteintech, Rosemont, USA). .. Cell viability and caspase 3/7 activation assay Cell viability and caspase -3 and -7 activation were determined using the CellTiter-Glo Luminescent Cell Viability Assay and Caspase-Glo 3/7 Assay (Promega), respectively.

Western Blot:

Article Title: AP39 alleviates HHCY-induced myocardial remodeling by regulating FUNDC1-mediated mitochondrial dynamics via S-sulfhydration of NEDD8/CUL4B
Article Snippet: .. All primary antibodies used for Western blotting (WB) and immunofluorescence (IF) were purchased from the corresponding commercial suppliers, and their detailed information is listed as follows: GAPDH (Proteintech, USA; 10494-1-AP; WB 1:8000), α-smooth muscle actin (α-SMA) (Proteintech, USA; 14395-1-AP; WB 1:4000), Collagen type III (Proteintech, USA; 22734-1-AP; WB 1:800), FUNDC1 (Proteintech, China; AWA51295 ; IF 1:100, WB 1:1000), LC3B (Proteintech, USA; 14600-1-AP; IF 1:500, WB 1:4000), MFN1 (Proteintech, USA; 13798-1-AP; WB 1:5000), MFN2 (Proteintech, USA; 12186-1-AP; WB 1:20000), DRP1 (Proteintech, USA; 12957-1-AP; WB 1:5000), P53 (Proteintech, USA; 10442-1-AP; WB 1:20000), P16 (Proteintech, USA; 10883-1-AP; WB 1:3000), NEDD8 (Abways, China; AWA11003 ; WB 1:1000), and CUL4B (Abways, China; AWA48229 ; WB 1:1000). .. For secondary antibodies, HRP-conjugated goat anti-mouse/goat anti-rabbit IgG (Proteintech, USA; SA00001-2; 1:5000) was used for Western blot detection.

Article Title: CSRP3 promotes skeletal muscle remodeling toward aerobic metabolism and enhances exercise endurance through increasing LDHD activity.
Article Snippet: Exercise performance and skeletal muscle homeostasis are influenced by myofiber type composition.. Cysteine and glycine-rich protein 3 (CSRP3) is highly expressed in the oxidative fiber-rich mammalian soleus muscle.. However, the mechanistic basis of CSRP3's involvement in skeletal muscle development and myofiber type specification remains unclear.

Immunofluorescence:

Article Title: AP39 alleviates HHCY-induced myocardial remodeling by regulating FUNDC1-mediated mitochondrial dynamics via S-sulfhydration of NEDD8/CUL4B
Article Snippet: .. All primary antibodies used for Western blotting (WB) and immunofluorescence (IF) were purchased from the corresponding commercial suppliers, and their detailed information is listed as follows: GAPDH (Proteintech, USA; 10494-1-AP; WB 1:8000), α-smooth muscle actin (α-SMA) (Proteintech, USA; 14395-1-AP; WB 1:4000), Collagen type III (Proteintech, USA; 22734-1-AP; WB 1:800), FUNDC1 (Proteintech, China; AWA51295 ; IF 1:100, WB 1:1000), LC3B (Proteintech, USA; 14600-1-AP; IF 1:500, WB 1:4000), MFN1 (Proteintech, USA; 13798-1-AP; WB 1:5000), MFN2 (Proteintech, USA; 12186-1-AP; WB 1:20000), DRP1 (Proteintech, USA; 12957-1-AP; WB 1:5000), P53 (Proteintech, USA; 10442-1-AP; WB 1:20000), P16 (Proteintech, USA; 10883-1-AP; WB 1:3000), NEDD8 (Abways, China; AWA11003 ; WB 1:1000), and CUL4B (Abways, China; AWA48229 ; WB 1:1000). .. For secondary antibodies, HRP-conjugated goat anti-mouse/goat anti-rabbit IgG (Proteintech, USA; SA00001-2; 1:5000) was used for Western blot detection.

Membrane:

Article Title: CSRP3 promotes skeletal muscle remodeling toward aerobic metabolism and enhances exercise endurance through increasing LDHD activity.
Article Snippet: Exercise performance and skeletal muscle homeostasis are influenced by myofiber type composition.. Cysteine and glycine-rich protein 3 (CSRP3) is highly expressed in the oxidative fiber-rich mammalian soleus muscle.. However, the mechanistic basis of CSRP3's involvement in skeletal muscle development and myofiber type specification remains unclear.

Pyrolysis Gas Chromatography:

Article Title: Di-(2-Ethylhexyl) phthalate causes testicular ferroptosis though deficiency of androgen receptor and disruption of mitochondrial quality control.
Article Snippet: Di (2-ethylhexyl) phthalate (DEHP) is a widely used plasticizer known to cause testicular toxicity, though its mechanism remains incompletely understood.. This study investigated whether DEHP-induced testicular injury involved suppression of androgen receptor (AR) expression.. In vivo, rats were exposed to DEHP (500 mg/kg) for 60 days, causing damage to the testicles.



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A , gene expression of fission and fusion markers Fis1, <t>Drp1,</t> OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.
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A , gene expression of fission and fusion markers Fis1, <t>Drp1,</t> OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.
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Image Search Results


USP18 aggravates cardiac I/R injury through regulation of mitochondria and inhibition of mitophagy. a Electron microscopy image showing mitophagy in USP18-cKO mouse hearts ( n= 5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. b Protein levels of PINK1, Parkin, ubiquitinated proteins (Ub), P62, and LC3II in mitochondria from heart tissue in USP18-cKO and WT mice 24 h after I/R injury ( n =4). c Electron microscopy image showing mitophagy in USP18-overexpres (OV) mouse hearts ( n =5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. d Protein levels of PINK1, Parkin, Ub, P62, and LC3II proteins in mitochondria from the heart tissue of USP18-OV mice 24 h after I/R injury ( n =4). Color shift in mitophagy dye (red) and lysosomal dye (green) in NRVMs showing mitophagy in NRVMs with USP18 siRNA transfection ( e ) or Ad-USP18 infection ( f ) and the quantitative mitophagy index in each group ( n= 5). Scale bar=9 μm. Protein levels of PINK1, Parkin, Ub, P62, and LC3II in mitochondria from NRVMs transfected with USP18 siRNA ( g ) or infected with Ad-USP18 ( h ). ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001 ⁎⁎⁎⁎ P <0.0001. USP18. Ubiquitin-specific protease 18; I/R. Ischemia/reperfusion; WT. Wild-type; KO. Knockout; P62. Sequestosome 1; LC3. Microtubule-associated protein 1 light chain 3; VDAC. Voltage-dependent anion channel.

Journal: Military Medical Research

Article Title: USP18 exacerbates myocardial I/R injury by inhibiting Parkin mitophagy through the deubiquitinase PTEN-L

doi: 10.1016/j.mmr.2026.100004

Figure Lengend Snippet: USP18 aggravates cardiac I/R injury through regulation of mitochondria and inhibition of mitophagy. a Electron microscopy image showing mitophagy in USP18-cKO mouse hearts ( n= 5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. b Protein levels of PINK1, Parkin, ubiquitinated proteins (Ub), P62, and LC3II in mitochondria from heart tissue in USP18-cKO and WT mice 24 h after I/R injury ( n =4). c Electron microscopy image showing mitophagy in USP18-overexpres (OV) mouse hearts ( n =5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. d Protein levels of PINK1, Parkin, Ub, P62, and LC3II proteins in mitochondria from the heart tissue of USP18-OV mice 24 h after I/R injury ( n =4). Color shift in mitophagy dye (red) and lysosomal dye (green) in NRVMs showing mitophagy in NRVMs with USP18 siRNA transfection ( e ) or Ad-USP18 infection ( f ) and the quantitative mitophagy index in each group ( n= 5). Scale bar=9 μm. Protein levels of PINK1, Parkin, Ub, P62, and LC3II in mitochondria from NRVMs transfected with USP18 siRNA ( g ) or infected with Ad-USP18 ( h ). ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001 ⁎⁎⁎⁎ P <0.0001. USP18. Ubiquitin-specific protease 18; I/R. Ischemia/reperfusion; WT. Wild-type; KO. Knockout; P62. Sequestosome 1; LC3. Microtubule-associated protein 1 light chain 3; VDAC. Voltage-dependent anion channel.

Article Snippet: To block mitophagy, the selective dynamin-related protein 1 (Drp1) inhibitor Mdivi-1 was used (50 μmol/L, MedChemExpress, USA).

Techniques: Inhibition, Electron Microscopy, Transfection, Infection, Ubiquitin Proteomics, Knock-Out

A , gene expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.

Journal: The Journal of Physiology

Article Title: Mitochondrial dynamics and repair differences in two mouse models of Friedreich's ataxia

doi: 10.1113/JP291356

Figure Lengend Snippet: A , gene expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.

Article Snippet: Primary antibodies: TFAM (Cell Signalling Technology, 8076), DRP1 (Cell Signalling Technology, 8570), FIS1 (Cell Signalling Technology, 32525), MFN2 (Abcam, ab56889), OPA1 (Cell Signalling Technology, 8047), Pink1 (Abcam, 23707), Parkin (Cell Signalling Technology, 32833), p62 (Abcam, ab56416), FUNDC1 (Cell Signalling Technology, 49240), BNIP3L (Cell Signalling Technology, 12396), LC3 (Cell Signalling Technology, 12 741), ATG7 (Cell Signalling Technology, 8558), ATG12–5 (Cell Signalling Technology, 4180), LAMP1 (Abcam, 24170), CD63 (Cell Signalling Technology, 10112), Cathepsin D (Cell Signalling, 74089), GAPDH (Abcam, ab8245), αTubulin (Millipore Sigma, T6199).

Techniques: Gene Expression, Western Blot, Expressing