SYBR Green Assay:Article Title: Dectin-1 Binding to Annexins on Apoptotic Cells Induces Peripheral Immune Tolerance via NADPH Oxidase-2.
Article Snippet: Geyer Cat# CAY10011659-250 sgp91 ds - tat, scrambled Anaspc Cat# AS-63821 gp91 ds - tat Anaspc Cat# AS-63818 GSK2795039 MedChem Express Cat# HY-18950 Collagenase Typ IV Biochrome Cat# C 4-28 DNase I Roche Cat# 10104159001 RPMI 1640 Sigma-Aldrich Cat# R8758-500ML FCS Sigma-Aldrich Cat# 12133C-500ML 2-Mercaptoethanol Sigma-Aldrich Cat# M6250 OPI-supplement Sigma-Aldrich Cat# O5003 Geneticin sulfate GERBU Biotechnik Cat# 1103,0250 Triton X-114 Sigma-Aldrich Cat# X114 Blasticidin S Hydrochloride Thermo Fisher Scientific Cat# R21001 Hygromycin B Gerbu Cat# 1312,1 Penicillin-Streptomycin Sigma-Aldrich Cat# P4333 Polymyxin B sulfate salt Sigma-Aldrich Cat# P4932 CpG ODN 1668 - TLR9 ligand Invivogen Cat# tlrl-1668 R848 (Resiquimod) Invivogen Cat# tlrl-r848-5 SYBR Green I nucleic acid gel stain Sigma-Aldrich Cat# S9430 H2DCFDA Sigma-Aldrich Cat# D6883 NAC Sigma-Aldrich Cat# A7250 PC Avanti Lipids Polar Cat# 850375 PS Avanti Lipids Polar Cat# 840035 Cy5-PE Avanti Lipids Polar Cat# 810335 Chloroform Sigma-Aldrich Cat# C2432 EndoFit Ovalbumin Invivogen Cat# vac-pova DPBS2+ Sigma-Aldrich Cat# D8662 Sucrose Merck Cat# S8501 GIBCO PBS, pH 7.4 Thermo Fisher Scientific Cat# 11503387 4% formaldehyde, methanol-free Thermo Fisher Scientific Cat# FB002 DAPI Merck Cat# 1246530100 AMV Reverse Transcriptase New England Biolabs Cat# M0277S p-nitrophenyl phosphate Thermo Fisher Scientific Cat# 34045 SYK-inhibitor-2 Merck Cat# 574712-1MG (Continued on next page) e2 Cell Reports 29, 4435–4446.e1–e9, December 24, 2019
Staining:Article Title: Dectin-1 Binding to Annexins on Apoptotic Cells Induces Peripheral Immune Tolerance via NADPH Oxidase-2.
Article Snippet: Geyer Cat# CAY10011659-250 sgp91 ds - tat, scrambled Anaspc Cat# AS-63821 gp91 ds - tat Anaspc Cat# AS-63818 GSK2795039 MedChem Express Cat# HY-18950 Collagenase Typ IV Biochrome Cat# C 4-28 DNase I Roche Cat# 10104159001 RPMI 1640 Sigma-Aldrich Cat# R8758-500ML FCS Sigma-Aldrich Cat# 12133C-500ML 2-Mercaptoethanol Sigma-Aldrich Cat# M6250 OPI-supplement Sigma-Aldrich Cat# O5003 Geneticin sulfate GERBU Biotechnik Cat# 1103,0250 Triton X-114 Sigma-Aldrich Cat# X114 Blasticidin S Hydrochloride Thermo Fisher Scientific Cat# R21001 Hygromycin B Gerbu Cat# 1312,1 Penicillin-Streptomycin Sigma-Aldrich Cat# P4333 Polymyxin B sulfate salt Sigma-Aldrich Cat# P4932 CpG ODN 1668 - TLR9 ligand Invivogen Cat# tlrl-1668 R848 (Resiquimod) Invivogen Cat# tlrl-r848-5 SYBR Green I nucleic acid gel stain Sigma-Aldrich Cat# S9430 H2DCFDA Sigma-Aldrich Cat# D6883 NAC Sigma-Aldrich Cat# A7250 PC Avanti Lipids Polar Cat# 850375 PS Avanti Lipids Polar Cat# 840035 Cy5-PE Avanti Lipids Polar Cat# 810335 Chloroform Sigma-Aldrich Cat# C2432 EndoFit Ovalbumin Invivogen Cat# vac-pova DPBS2+ Sigma-Aldrich Cat# D8662 Sucrose Merck Cat# S8501 GIBCO PBS, pH 7.4 Thermo Fisher Scientific Cat# 11503387 4% formaldehyde, methanol-free Thermo Fisher Scientific Cat# FB002 DAPI Merck Cat# 1246530100 AMV Reverse Transcriptase New England Biolabs Cat# M0277S p-nitrophenyl phosphate Thermo Fisher Scientific Cat# 34045 SYK-inhibitor-2 Merck Cat# 574712-1MG (Continued on next page) e2 Cell Reports 29, 4435–4446.e1–e9, December 24, 2019
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