Journal: Scientific Reports

Article Title: Pseudolaric acid B promotes lung cancer cells ferroptosis depending on JNK/ERK-mediated upregulation of survivin

doi: 10.1038/s41598-026-36423-3

Figure Lengend Snippet: PAB induces ferroptosis in lung cancer cells. ( A ) Volcano plots of differentially expressed genes. ( B ) The heat map mainly exhibits the expression levels of 10 up-regulated and 10 down-regulated differentially expressed genes (> 2.0-fold). ( C ) Representative transmission electron microscopy images show that the A549 cell is treated with PAB (10 µM) for 12 h. Scale bars, 2 μm. ( D ) Representative images of the impacts of PAB on mitochondrial morphology in A549 and H460 cells follow 12 h treated, with mitochondria labeled by Mito tracker. Scale bars, 5 μm. ( E ) The changes in mitochondrial reactive oxygen species in A549 and H460 cells treated with PAB (10 µM) for 0, 4, 8, 12 and 24 h are detected by mtSOX red probe. ( F , L ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of DFO (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( G , M ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB (10 µM) stimulation of A549 and H460 cells in the presence or absence of DFO (10 µM). The red color indicated non-oxidized form while green color indicated oxidized form. Scale bar, 20 μm. ( H ) Western blotting analysis is performed on the lysates of A549 and H460 cells treated with PAB (0, 2.5, 5, 10 µM) for 36 h. ( I ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of DFO (10 µM) is determined by CCK8 assay. ( J ) Cell viability of A549 and H460 cells following 72 h treatment with PAB (10 µM), either alone or in combination with DFO (10 µM), is assessed using crystal violet staining. ( K ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of DFO (10 µM) is determined. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.

Article Snippet: PAB (HY-N6939), DFO (HY-B0568), SP600125 (HY-12041), U0126 (HY-12031 A), YM155 (HY-10194), z-VAD-FMK (HY-16658B), CQ (HY-17589 A) were purchased from MedChem Express (Shanghai, China).

Techniques: Expressing, Transmission Assay, Electron Microscopy, Labeling, Extraction, Western Blot, CCK-8 Assay, Staining, Control

Journal: Parasites & Vectors

Article Title: Toxoplasma gondii -induced ferroptosis contributes to acute lung injury in mice

doi: 10.1186/s13071-025-07207-x

Figure Lengend Snippet: Deferiprone reduces T. gondii -induced oxidative stress, iron overload, and mitochondrial damage in mouse lungs. ( A) Schematic of the experimental design for T. gondii infection and DFP treatment. From −1 to 6 dpi, mice in the NC and RH groups received water (i.g.), whereas the DFP and DFP + RH group mice received DFP (100 mg/kg, i.g.); mice in the RH and DFP + RH groups were injected i.p. with 1 × 10 3 tachyzoites; n = 5 mice per group. ( B) Serum iron concentration. The levels of MDA ( C) , GSH ( D) , total iron ( E) , and Fe 2+ ( F) in lungs of mice. ( G) Prussian blue staining of lung sections from mice at 6 dpi. Green arrows denote iron deposition (blue granules). Scale bar = 50 μm. ( H) Representative TEM images of mitochondrial ultrastructures in lungs. Top: low-magnification overview (scale bar = 2 μm). Bottom: high-magnification view of boxed region (N, nucleus; Mi, mitochondria; black arrows point to mitochondrial shrinkage and cristae rupture. Scale bar = 1 μm). Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparison test

Article Snippet: In the DFP group and DFP + RH group, mice were intragastric administrated (i.g.) daily with 100 mg/kg DFP (an inhibitor of ferroptosis; cat. no. HY-B0568; MCE, New Jersey, USA); then in the DFP + RH group mice, were challenged with 1 × 10 3 tachyzoites i.p. on the second day after the first administration of DFP.

Techniques: Infection, Injection, Concentration Assay, Staining, Comparison

Journal: Parasites & Vectors

Article Title: Toxoplasma gondii -induced ferroptosis contributes to acute lung injury in mice

doi: 10.1186/s13071-025-07207-x

Figure Lengend Snippet: Antioxidant and iron metabolism pathways in T. gondii- induced lung ferroptosis were modulated by DFP. Western blot analysis of ( A ) antioxidant proteins (NRF2, SLC7A11, HO-1, and GPX4) and ( B ) iron metabolism proteins (TFR1, FPN, DMT1, ferritin) in mouse lung tissues. Relative gray values of ( C ) TFR1, ( D ) FPN, ( E ) DMT1, ( F ) ferritin, ( G ) NRF2, ( H ) SLC7A11, ( I ) HO-1, and ( J ) GPX4 expression were normalized to β-actin by ImageJ. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparison test

Article Snippet: In the DFP group and DFP + RH group, mice were intragastric administrated (i.g.) daily with 100 mg/kg DFP (an inhibitor of ferroptosis; cat. no. HY-B0568; MCE, New Jersey, USA); then in the DFP + RH group mice, were challenged with 1 × 10 3 tachyzoites i.p. on the second day after the first administration of DFP.

Techniques: Western Blot, Expressing, Comparison

Journal: Parasites & Vectors

Article Title: Toxoplasma gondii -induced ferroptosis contributes to acute lung injury in mice

doi: 10.1186/s13071-025-07207-x

Figure Lengend Snippet: Toxoplasma gondii -induced mortality and lung pathology were mitigated by DFP treatment. ( A) Body weight changes were recorded daily for 10 days. ( B) Survival curves were monitored for 10 days. ( C) Parasite burden in lungs from RH- and DFP + RH-treated mice were quantified by qPCR. The inflammatory cytokines IL-12 ( D) , IL-6 ( E) , TNF-α ( F) , and IFN-γ ( G) were measured by ELISA ( n = 5 mice/group). ( H) Representative H&E staining of lung sections from mice were performed. Black arrows indicate alveolar hemorrhage and alveolar wall thickening. Top: low-magnification (scale bar = 100 μm). Bottom: high-magnification (scale bar = 50 μm). Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined by two-way ANOVA with Šidák’s multiple comparisons test ( A ), log-rank test ( B ), unpaired two-tailed Student’s t -test ( C ), or one-way ANOVA with Tukey’s multiple comparison test ( D - G )

Article Snippet: In the DFP group and DFP + RH group, mice were intragastric administrated (i.g.) daily with 100 mg/kg DFP (an inhibitor of ferroptosis; cat. no. HY-B0568; MCE, New Jersey, USA); then in the DFP + RH group mice, were challenged with 1 × 10 3 tachyzoites i.p. on the second day after the first administration of DFP.

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Two Tailed Test, Comparison