Journal: Scientific Reports

Article Title: Pseudolaric acid B promotes lung cancer cells ferroptosis depending on JNK/ERK-mediated upregulation of survivin

doi: 10.1038/s41598-026-36423-3

Figure Lengend Snippet: PAB induces ferroptosis in lung cancer cells. ( A ) Volcano plots of differentially expressed genes. ( B ) The heat map mainly exhibits the expression levels of 10 up-regulated and 10 down-regulated differentially expressed genes (> 2.0-fold). ( C ) Representative transmission electron microscopy images show that the A549 cell is treated with PAB (10 µM) for 12 h. Scale bars, 2 μm. ( D ) Representative images of the impacts of PAB on mitochondrial morphology in A549 and H460 cells follow 12 h treated, with mitochondria labeled by Mito tracker. Scale bars, 5 μm. ( E ) The changes in mitochondrial reactive oxygen species in A549 and H460 cells treated with PAB (10 µM) for 0, 4, 8, 12 and 24 h are detected by mtSOX red probe. ( F , L ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of DFO (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( G , M ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB (10 µM) stimulation of A549 and H460 cells in the presence or absence of DFO (10 µM). The red color indicated non-oxidized form while green color indicated oxidized form. Scale bar, 20 μm. ( H ) Western blotting analysis is performed on the lysates of A549 and H460 cells treated with PAB (0, 2.5, 5, 10 µM) for 36 h. ( I ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of DFO (10 µM) is determined by CCK8 assay. ( J ) Cell viability of A549 and H460 cells following 72 h treatment with PAB (10 µM), either alone or in combination with DFO (10 µM), is assessed using crystal violet staining. ( K ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of DFO (10 µM) is determined. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.

Article Snippet: PAB (HY-N6939), DFO (HY-B0568), SP600125 (HY-12041), U0126 (HY-12031 A), YM155 (HY-10194), z-VAD-FMK (HY-16658B), CQ (HY-17589 A) were purchased from MedChem Express (Shanghai, China).

Techniques: Expressing, Transmission Assay, Electron Microscopy, Labeling, Extraction, Western Blot, CCK-8 Assay, Staining, Control

Journal:

Article Title: Antifungal Activities of Natural and Synthetic Iron Chelators Alone and in Combination with Azole and Polyene Antibiotics against Aspergillus fumigatus ▿

doi: 10.1128/AAC.01547-08

Figure Lengend Snippet: Iron chelator effect on Aspergillus fumigatus conidial growth. Fungal growth inhibition was performed as described in the text, and the data are expressed as percentages of control growth in vehicle alone. Dose-response curves were estimated from multiple experiments (for deferoxamine, n = 3; for deferiprone, n = 38; for ciclopirox, n = 40; for lactoferrin, n = 43) using three-parameter logistic models and are plotted with 95% confidence intervals.

Article Snippet: As shown in Fig. , lactoferrin (partially saturated native human; Sigma Chemicals, St. Louis, MO) was most potent on a molar basis, requiring only 105 ± 9 nM to reduce untreated control growth by 50% (50% inhibitory concentration [IC 50 ]), followed by ciclopirox (6-cyclohexyl-1-hydroxy-4-methyl-2(1H)-pyridinone; Toronto Research Chemicals, North York, Ontario, Canada) (IC 50 = 4.22 ± 0.18 μM) and deferiprone (1,2-dimethyl-3-hydroxypyrid-4-one; Toronto Research Chemicals) (IC 50 = 1.29 ± 0.2 mM).

Techniques: Inhibition, Control

Journal: bioRxiv

Article Title: An Iron-regulated Signalling Pathway Controls Adipose Browning and Cancer Cachexia

doi: 10.1101/2025.07.16.664180

Figure Lengend Snippet: MSRA is stabilized upon iron binding. a , Immunoblot analysis of sWAT from mice treated with vehicle, 5 mg/kg β3-adrenergic agonist (CL-316243), 50 mg/kg deferiprone (DFP), or in combination (CL+DFP) for 6 days. b , Cycloheximide (CHX) pulse-chase to measure MSRA protein half-life in white adipocyte cultures treated with saline or ferric ammonium citrate (FAC). Right, quantification of MSRA relative to VINCULIN. c - g , Absorbance properties of recombinant human MSRA in the presence of different elements. Inset, zoomed-in spectra of MSRA in the presence of ferric ammonium citrate (FAC). 5eq, five molar equivalents in concentration. 10eq, ten molar equivalents in concentration. h, Inductively coupled plasma mass spectrometry (ICP-MS) is used to measure intracellular trace elements that are enriched in MSRA immunoprecipitants.

Article Snippet: To study the effects of β3-adrenergic receptor activation and iron chelation, the β3-adrenergic receptor agonist CL-316243 disodium salt (Cayman Chemical, Cat#17499) was systemically administered by daily intraperitoneal injection (5 mg/kg in saline), and deferiprone (EDQM, Cat#Y0001976) was administered by oral gavage (50 mg/kg in water).

Techniques: Binding Assay, Western Blot, Pulse Chase, Saline, Recombinant, Concentration Assay, Clinical Proteomics, Mass Spectrometry