daudi cell  (ATCC)

Journal: bioRxiv

Article Title: CRISPR Screens Reveal Epstein-Barr Virus-activated JunB as a Key Lymphoblastoid B cell Dependency Factor that Represses Cyclin Dependent Kinase Inhibitor P18INK4c

doi: 10.64898/2026.03.31.715635

Figure Lengend Snippet: (A) CCND2 sgRNA abundances in Brunello P3HR-1 and GM12878 dropout screens. Log 2 CCND2 sgRNA abundances from GM12878 (red) and P3HR-1 (blue) cells 21 days post library selection were determined via deep sequencing and compared to input library sgRNA abundance (green). (B) CCND2 transcript levels during EBV-mediated B cell transformation. RNAseq was performed on primary B cells infected with B95-8 virus on indicated days post infection (dpi). Normalized reads for CCND2 are shown. (C) Immunoblot analysis of CCND2 levels in response to LMP1 expression. Daudi Burkitt cells harboring selectively inducible WT or transformation effector site (TES) mutant LMP1 cassettes were mock induced or treated with 250 ng / mL doxycycline for 24 hours. Cells were then harvested and lysates analyzed via immunoblot with indicated antibodies. (D) Effect of JunB knockout on CCND2 levels. Cas9+ P3HR-1, Jijoye, or GM12878 cells expressing control or JunB sgRNAs. Seven days post selection, WCL was extracted from cells and immunoblot performed using indicated antibodies. P-values were determined by one-sided Fisher’s exact test. * p<0.05, **p<0.005, ***p<0.0005

Article Snippet: 293T, Daudi and Jijoye were purchased from American Type Culture Collection.

Techniques: Selection, Sequencing, Transformation Assay, RNA sequencing, Infection, Virus, Western Blot, Expressing, Mutagenesis, Knock-Out, Control