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c1005 staining solution  (Beyotime)


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    Structured Review

    Beyotime c1005 staining solution
    C1005 Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2090 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+solution/DAPI+Staining+Solution/pm42880593-220-13-19
    Average 99 stars, based on 2090 article reviews
    c1005 staining solution - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: CTCF-FXN regulatory axis plays critical roles in clinical assessments and disease progression of primary open-angle glaucoma
    Article Snippet: .. Cells were moved into 4-well chamber slide and were incubated with TUNEL reaction mixture (Absin, China) in dark for 1 h. Nucleus was stained with DAPI solution (Beyotime, China) at 37 °C for 5 min. ..

    Article Title: Camellia nitidissima Flower Extract Alleviates Stress-Induced Sebaceous Dysfunction by Targeting the 11β-HSD1/PI3K/Akt/mTOR Axis.
    Article Snippet: .. Briefly, cells were incubated with 100 μL of DAPI solution (Beyotime Inc., Shanghai, China) for 10 min at room temperature to stain the nuclei. .. After removing the DAPI solution, 100 μL of Nile Red solution (10 μg/mL in PBS) was added to each well and incubated at 37 ◦C in the dark for 20 min. Stained cells were imaged using a fluorescence microscope (Leica Stellaris 5, Germany), and the fluorescence intensity of Nile Red was measured at excitation and emission wavelengths of 485 nm and 565 nm, respectively.

    Article Title: CTCF-FXN regulatory axis plays critical roles in clinical assessments and disease progression of primary open-angle glaucoma.
    Article Snippet: .. Cells were moved into 4-well chamber slide and were incubated with TUNEL reaction mixture (Absin, China) in dark for 1 h. Nucleus was stained with DAPI solution (Beyotime, China) at 37 °C for 5 min. ..

    TUNEL Assay:

    Article Title: CTCF-FXN regulatory axis plays critical roles in clinical assessments and disease progression of primary open-angle glaucoma
    Article Snippet: .. Cells were moved into 4-well chamber slide and were incubated with TUNEL reaction mixture (Absin, China) in dark for 1 h. Nucleus was stained with DAPI solution (Beyotime, China) at 37 °C for 5 min. ..

    Article Title: Electroacupuncture alleviates ferroptosis through miR-145-5p/ACSL4 axis to reduce post-reperfusion brain injury in ischemic stroke.
    Article Snippet: 20 μg/mL of Protease K (HY-108717A, MedChemExpress) without DNase was applied to the surface of the slice for 15 min at 37 °C. .. After being fully rinsed using PBS, tissue sections were covered with TUNEL detection solution (C1086, Beyotime) for 60 min in the dark, then covered with DAPI solution (C1006, Beyotime) for 15 min, and viewed through DM3000 fluorescence microscope (Leica). .. 2.8 Dihydroethidium (DHE) staining Fresh brain tissue of mice was taken and immersed in OCT embedding agent (C0171A, Beyotime) to ensure complete coverage of the tissue.

    Article Title: CTCF-FXN regulatory axis plays critical roles in clinical assessments and disease progression of primary open-angle glaucoma.
    Article Snippet: .. Cells were moved into 4-well chamber slide and were incubated with TUNEL reaction mixture (Absin, China) in dark for 1 h. Nucleus was stained with DAPI solution (Beyotime, China) at 37 °C for 5 min. ..

    Staining:

    Article Title: CTCF-FXN regulatory axis plays critical roles in clinical assessments and disease progression of primary open-angle glaucoma
    Article Snippet: .. Cells were moved into 4-well chamber slide and were incubated with TUNEL reaction mixture (Absin, China) in dark for 1 h. Nucleus was stained with DAPI solution (Beyotime, China) at 37 °C for 5 min. ..

    Article Title: Camellia nitidissima Flower Extract Alleviates Stress-Induced Sebaceous Dysfunction by Targeting the 11β-HSD1/PI3K/Akt/mTOR Axis.
    Article Snippet: .. Briefly, cells were incubated with 100 μL of DAPI solution (Beyotime Inc., Shanghai, China) for 10 min at room temperature to stain the nuclei. .. After removing the DAPI solution, 100 μL of Nile Red solution (10 μg/mL in PBS) was added to each well and incubated at 37 ◦C in the dark for 20 min. Stained cells were imaged using a fluorescence microscope (Leica Stellaris 5, Germany), and the fluorescence intensity of Nile Red was measured at excitation and emission wavelengths of 485 nm and 565 nm, respectively.

    Article Title: CTCF-FXN regulatory axis plays critical roles in clinical assessments and disease progression of primary open-angle glaucoma.
    Article Snippet: .. Cells were moved into 4-well chamber slide and were incubated with TUNEL reaction mixture (Absin, China) in dark for 1 h. Nucleus was stained with DAPI solution (Beyotime, China) at 37 °C for 5 min. ..

    Article Title: Bioactive PCL/nSi-BMSCs composite scaffold regulates periosteal cell functions for neurovascularized bone regeneration
    Article Snippet: .. Similarly, PCs were fixed, permeabilized, and blocked to be stained with rhodamine phalloidin (1:200; Beyotime, China) and DAPI solution (Beyotime, China). .. Next, using fluorescence microscopy (Zeiss, Germany) to view the stained cells.

    Saline:

    Article Title: Kaempferol ameliorates gestational diabetes mellitus by regulating STAT3-associated AIM2 inflammasome activation and ferroptotic injury in trophoblasts
    Article Snippet: Nuclear staining was performed using 4’,6-diamidino-2-phenylindole (DAPI, GDP1024, Servicebio), followed by a 10-minute incubation at room temperature. .. After removing the DAPI solution, the sections were washed twice with phosphate-buffered saline (PBS, ST447-1L, Beyotime) and then mounted with glycerin. ..

    Immunofluorescence:

    Article Title: Panaxytriol up-regulates CYP2B6 expression through PXR and CAR and their interactions with RXRα and SRC-1.
    Article Snippet: 2 3 Ethnopharmacological relevance: Cytochrome P450 2B6 (CYP2B6) is a critical drug2 4 metabolizing enzyme, and its expression is regulated by the nuclear receptors.. Panaxytriol 2 5 (PXT), a prominent bioactive constituent extracted from the roots of Panax ginseng C.A.. Meyer, 2 6 was found to induce cytochrome P450 3A4 (CYP3A4) via pregnane X receptor (PXR) and 2 7 constitutive androstane receptor (CAR) in our previous work.

    Confocal:

    Article Title: Panaxytriol up-regulates CYP2B6 expression through PXR and CAR and their interactions with RXRα and SRC-1.
    Article Snippet: 2 3 Ethnopharmacological relevance: Cytochrome P450 2B6 (CYP2B6) is a critical drug2 4 metabolizing enzyme, and its expression is regulated by the nuclear receptors.. Panaxytriol 2 5 (PXT), a prominent bioactive constituent extracted from the roots of Panax ginseng C.A.. Meyer, 2 6 was found to induce cytochrome P450 3A4 (CYP3A4) via pregnane X receptor (PXR) and 2 7 constitutive androstane receptor (CAR) in our previous work.

    Laser-Scanning Microscopy:

    Article Title: Panaxytriol up-regulates CYP2B6 expression through PXR and CAR and their interactions with RXRα and SRC-1.
    Article Snippet: 2 3 Ethnopharmacological relevance: Cytochrome P450 2B6 (CYP2B6) is a critical drug2 4 metabolizing enzyme, and its expression is regulated by the nuclear receptors.. Panaxytriol 2 5 (PXT), a prominent bioactive constituent extracted from the roots of Panax ginseng C.A.. Meyer, 2 6 was found to induce cytochrome P450 3A4 (CYP3A4) via pregnane X receptor (PXR) and 2 7 constitutive androstane receptor (CAR) in our previous work.

    Tissue:

    Article Title: Electroacupuncture alleviates ferroptosis through miR-145-5p/ACSL4 axis to reduce post-reperfusion brain injury in ischemic stroke.
    Article Snippet: 20 μg/mL of Protease K (HY-108717A, MedChemExpress) without DNase was applied to the surface of the slice for 15 min at 37 °C. .. After being fully rinsed using PBS, tissue sections were covered with TUNEL detection solution (C1086, Beyotime) for 60 min in the dark, then covered with DAPI solution (C1006, Beyotime) for 15 min, and viewed through DM3000 fluorescence microscope (Leica). .. 2.8 Dihydroethidium (DHE) staining Fresh brain tissue of mice was taken and immersed in OCT embedding agent (C0171A, Beyotime) to ensure complete coverage of the tissue.

    Fluorescence:

    Article Title: Electroacupuncture alleviates ferroptosis through miR-145-5p/ACSL4 axis to reduce post-reperfusion brain injury in ischemic stroke.
    Article Snippet: 20 μg/mL of Protease K (HY-108717A, MedChemExpress) without DNase was applied to the surface of the slice for 15 min at 37 °C. .. After being fully rinsed using PBS, tissue sections were covered with TUNEL detection solution (C1086, Beyotime) for 60 min in the dark, then covered with DAPI solution (C1006, Beyotime) for 15 min, and viewed through DM3000 fluorescence microscope (Leica). .. 2.8 Dihydroethidium (DHE) staining Fresh brain tissue of mice was taken and immersed in OCT embedding agent (C0171A, Beyotime) to ensure complete coverage of the tissue.

    Microscopy:

    Article Title: Electroacupuncture alleviates ferroptosis through miR-145-5p/ACSL4 axis to reduce post-reperfusion brain injury in ischemic stroke.
    Article Snippet: 20 μg/mL of Protease K (HY-108717A, MedChemExpress) without DNase was applied to the surface of the slice for 15 min at 37 °C. .. After being fully rinsed using PBS, tissue sections were covered with TUNEL detection solution (C1086, Beyotime) for 60 min in the dark, then covered with DAPI solution (C1006, Beyotime) for 15 min, and viewed through DM3000 fluorescence microscope (Leica). .. 2.8 Dihydroethidium (DHE) staining Fresh brain tissue of mice was taken and immersed in OCT embedding agent (C0171A, Beyotime) to ensure complete coverage of the tissue.



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    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were <t>counterstained</t> with <t>DAPI.</t> ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.
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    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were <t>counterstained</t> with <t>DAPI.</t> ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.
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    Image Search Results


    Journal: iScience

    Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa

    doi: 10.1016/j.isci.2026.117730

    Figure Lengend Snippet:

    Article Snippet: Finally, sections were counterstained with Diamidin-2-phenylindol (DAPI, ACD Bio-Techne GmbH) for 30 seconds at RT and mounted directly using ProLong Gold antifade mounting medium (Thermo Fisher Scientific, USA).

    Techniques: Plasmid Preparation, Virus, Indirect ELISA, Recombinant, RNAscope, Multiplex Assay, Software, Negative Control, Confocal, Laser-Scanning Microscopy

    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Journal: International Journal of Nanomedicine

    Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

    doi: 10.2147/IJN.S645818

    Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

    Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing