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6 diamidino 2 phenylindole  (Beyotime)


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    Structured Review

    Beyotime 6 diamidino 2 phenylindole
    6 Diamidino 2 Phenylindole, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+solution/Beyo3D+DAPI+Staining+Solution/pm42908353-49-46-47
    Average 99 stars, based on 1 article reviews
    6 diamidino 2 phenylindole - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: All-Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27-Mediated Autophagy Modulation.
    Article Snippet: .. For immunofluorescence labeling, cells were blocked for 10min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6-diamidino-2-phenylindole (Beyotime) for 5min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Labeling:

    Article Title: All-Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27-Mediated Autophagy Modulation.
    Article Snippet: .. For immunofluorescence labeling, cells were blocked for 10min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6-diamidino-2-phenylindole (Beyotime) for 5min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Blocking Assay:

    Article Title: All-Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27-Mediated Autophagy Modulation.
    Article Snippet: .. For immunofluorescence labeling, cells were blocked for 10min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6-diamidino-2-phenylindole (Beyotime) for 5min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Staining:

    Article Title: All-Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27-Mediated Autophagy Modulation.
    Article Snippet: .. For immunofluorescence labeling, cells were blocked for 10min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6-diamidino-2-phenylindole (Beyotime) for 5min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).

    Incubation:

    Article Title: All-Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27-Mediated Autophagy Modulation.
    Article Snippet: .. For immunofluorescence labeling, cells were blocked for 10min at room temperature (QuickBlock Blocking Buffer for Immunol Staining, Beyotime, Shanghai, China) and then incubated overnight at 4°C with the primary antibody and for 1 h at room temperature with the secondary antibody; nuclei were stained with 4′, 6-diamidino-2-phenylindole (Beyotime) for 5min. .. The fluorescence signal was observed with a confocal laser scanning microscope (SP8 TCS; Leica, Germany).



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    Beyotime antifade mounting solution
    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were <t>counterstained</t> with <t>DAPI.</t> ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.
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    Image Search Results


    Journal: iScience

    Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa

    doi: 10.1016/j.isci.2026.117730

    Figure Lengend Snippet:

    Article Snippet: Finally, sections were counterstained with Diamidin-2-phenylindol (DAPI, ACD Bio-Techne GmbH) for 30 seconds at RT and mounted directly using ProLong Gold antifade mounting medium (Thermo Fisher Scientific, USA).

    Techniques: Plasmid Preparation, Virus, Indirect ELISA, Recombinant, RNAscope, Multiplex Assay, Software, Negative Control, Confocal, Laser-Scanning Microscopy

    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Journal: International Journal of Nanomedicine

    Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

    doi: 10.2147/IJN.S645818

    Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

    Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing