d1r agonist (Merck & Co)
86
Structured Review
Merck & Co
d1r agonist
D1r Agonist, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/d1r agonist/product/Merck & Co
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
D1r Agonist, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/d1r agonist/product/Merck & Co
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
d1r agonist - by Bioz Stars,
2024-10
86/100 stars
Images
1) Product Images from "Behavior- and circuit-specific cortico-striatal decoupling during the early development of Parkinson’s disease-like syndrome"
Article Title: Behavior- and circuit-specific cortico-striatal decoupling during the early development of Parkinson’s disease-like syndrome
Journal: bioRxiv
doi: 10.1101/2024.08.13.607859
Figure Legend Snippet: A . Schematic diagram showing the experimental design for analyzing cell type-specific cortico-striatal coupling. B . Immunofluorescence images showing the colocalization between DRD1 (red) and DIO-GCaMP (green) in D1R-Cre mice (top panels), and the colocalization between DRD2 (magenta) and DIO-GCaMP (green) in the D2R-Cre mice (bottom panels). Scale bar: 10μm. C, E, G . Z-scored average traces of M1 (red) and STR dMSN (light green) and iMSN (dark green) Ca 2+ activities temporally aligned to the initiation of contralateral turning (C), licking (E), and digging (G). D, F, H . Comparison of cell type-specific cortico-striatal coupling indices for turning (D), licking (F), and digging behavior (H, P = 0.0087). Mann-Whitney test. ** P <0.01.
Techniques Used: Immunofluorescence, Comparison, MANN-WHITNEY
Figure Legend Snippet: A-E. Dual fiber photometry imaging of M1 and striatal dMSN in PFF- and PBS-injected mice at 2 Mpi (B-C), and 3-4 Mpi (D-E) during digging. (A) Scheme of virus injection and optic fiber implantation. Note the CreON-GCaMP virus was injected into the STR of D1R-Cre mice, while CreOFF-GCaMP virus was injected into the STR of D2R-Cre mice. Right panel, experiment timeline. (B, D) Z-scored average traces of M1 (red) and STR dMSN (aqua) Ca 2+ activities temporally aligned to the initiation of digging. Left, PBS; Right, PFF. (C, E) Quantitative analyses of the M1-dMSN coupling and plateau amplitude of dMSN Ca 2+ activities at 2 Mpi (C) and 3-4 Mpi (E). The hollow signal represents D1R-Cre mice; the cross signal represents D2-Cre mice. (C1, E1) Coupling indices (C1, P = 0.0043. E1, P = 0.0062). (C2, E2) Phase lags (E2, P = 0.0043. G2, P = 0.0062). (C3, E3) Plateau amplitude of dMSN Ca 2+ activities (E3, P = 0.0451). F-J. Dual fiber photometry imaging of M1 and striatal iMSN in PFF- and PBS-injected mice at 2 Mpi (G-H), and 3-4 Mpi (I-J) during digging. Right panel, experiment timeline. (F) Scheme of virus injection and optic fiber implantation. (G, I) Z-scored average traces of M1 (red) and striatal iMSN (dark green) Ca 2+ activities temporally aligned to the initiation of digging. Left, PBS; Right, PFF. (H, J) Quantitative analyses of the M1-iMSN coupling and plateau amplitude of iMSN Ca 2+ activities at 2 Mpi (H) and 3-4 Mpi (J). (H1, J1) Comparison of the coupling indices. (H2, J2) Comparison of the phase lags. (H3, J3) Comparison of the plateau amplitude of iMSN Ca 2+ activities. Mann-Whitney test. * P <0.05. ** P <0.01.
Techniques Used: Imaging, Injection, Virus, Comparison, MANN-WHITNEY
Figure Legend Snippet: A. Schematic diagram for pharmacological experiments. i.p., intraperitoneal administration. B-E. Dual fiber photometry imaging of M1 and STR in PFF- and PBS-injected mice at 4Mpi during digging, after ip. SAL (saline), L-dopa, SKF (SKF-81297, a D1R selective agonist), or Quin (quinpirole, a D2R selective agonist). (B) Z-scored average traces of M1 (red) and STR (green) temporally aligned, digging-associated Ca 2+ activities after administration of drugs. Left, PBS; Right, PFF. (C, D, E) Quantitative analyses of the digging-associated cortico-striatal coupling (C,D) and amplitude of striatal Ca 2+ activities (E) after administration of drugs. (C1) Comparison of the phase lags (SAL-PBS vs. SAL-PFF: Mann-Whitney test, P = 0.0152. PFF groups: SAL-PFF as control, F(1.588, 9.529) = 9.270, SAL-PFF vs. Ldopa-PFF, P =0.0156; SAL-PFF vs. SKF-PFF, P = 0.0328). (C2) Comparison of the phase lags, after saline or quinpirole administration. (D1) Comparison of the coupling indices (SAL-PBS vs. SAL-PFF: Mann-Whitney test, P = 0.0043). (D2) Comparison of the coupling indices, after saline or quinpirole administration. (E1) Comparison of the plateau amplitude of striatal activity (SAL-PBS vs. SAL-PFF: Mann-Whitney test, P = 0.0424. PFF groups: SAL-PFF as control, F(1.453, 8.719) = 6.152, SAL-PFF vs. SKF-PFF, P = 0.0459). (E2) Comparison of the plateau amplitude of striatal activity, after saline or quinpirole administration. F-G. Effect of dopaminergic drugs on digging behavior. (F) Comparison of digging duration (PFF groups: SAL-PFF as control, F(1.463, 7.317) = 19.97, SAL-PFF vs. Ldopa-PFF, P = 0.0018; SAL-PFF vs. SKF-PFF, P = 0.0388). (G) Comparison of digging frequency (PFF groups: SAL-PFF as control, F(2.120, 10.60) = 11.78, SAL-PFF vs. SKF-PFF, P = 0.0143; SAL-PFF vs. Quin-PFF, P = 0.0192). One-way ANOVA followed by Dunnett’s multiple comparisons test, unless otherwise noted. Wilcoxon matched-pairs test (C2, D2, E2). * P <0.05. ** P <0.01.
Techniques Used: Imaging, Injection, Saline, Comparison, MANN-WHITNEY, Control, Activity Assay