2 d08 (MedChemExpress)
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2 d08
2 D08, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d08/2-D08/pmc12996142-43-4-15
Average 94 stars, based on 17 article reviews
2 D08, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d08/2-D08/pmc12996142-43-4-15
Average 94 stars, based on 17 article reviews
2 d08 - by Bioz Stars,
2026-09
94/100 stars
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1) Product Images from "CBX4 facilitates EV71 replication by SUMOylation and stabilizing 3D polymerase"
Article Title: CBX4 facilitates EV71 replication by SUMOylation and stabilizing 3D polymerase
Journal: Frontiers in Microbiology
doi: 10.3389/fmicb.2026.1775950
Figure Legend Snippet: SUMOylation inhibitor 2-D08 suppresses EV71 replication. (A) RD cells were added with 0, 20, 50, and 100 μΜ 2-D08 for 24 h, the cell viability was determined by CCK8 assay. The results were shown as percentage relative to control. (B–E) RD or HT29 cells were treated with EV71 (MOI = 1) and 2-D08 (0, 20, 50, 100 μM), after 16 h, the supernatants and cells were collected. The expression levels of CBX4, 3D, VP1, 3C and GAPDH proteins were detected by Western blotting (B) , the mRNA level of VP1 was determined by qPCR assay (C) , the viral titers in supernatants from RD (D) or HT29 (E) cells were measured by plaque assay. (F) HEK293T cells were transfected with HA-3D plasmid for 6 h, and then treated with different concentrations of 2-D08 (0, 20, 50, 100 μM) for 24 h. The protein levels of HA-3D and GAPDH were detected by immunoblotting. (G) HEK293T cells were transfected with GFP-3D, Myc-SUMO1, HA-Ubc9 and HA-CBX4 plasmids for 6 h, and then treated with 2-D08 (50 μM) for 24 h. The cell lysates were immunoprecipitated with anti-GFP immunomagnetic beads. The IP and input complex were analyzed with indicated antibodies. The graphs are shown as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.
Techniques Used: CCK-8 Assay, Control, Expressing, Western Blot, Plaque Assay, Transfection, Plasmid Preparation, Immunoprecipitation
Figure Legend Snippet: A model illustrating how CBX4 promotes EV71 replication. Host SUMO E3 ligase CBX4 interacts with EV71-encoded 3D polymerase and induces the SUMOylation of 3D, which results in the increased stability of 3D protein and EV71 replication. Pharmacological inhibition of protein SUMOylation with 2-D08 diminishes the SUMOylation and expression of 3D, and ultimately affects the replication of EV71.
Techniques Used: Inhibition, Expressing
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The SUMO inhibitors TAK-981 and Article Title: Comprehensive SUMO Proteomic Analyses Identify HIV Latency-Associated Proteins in Microglia Article Snippet: western blot; IF, immunofluorescence. .. The SUMO inhibitors TAK-981 and Article Title: Insights into degradation and targeting of the photoreceptor channelrhodopsin-1. Article Snippet: 3‐Isobutyl‐1‐methylxanthine (IBMX), 4‐hydroxy‐2,2,6,6‐tetramethyl‐piperidine 1‐oxyl (TEMPOL), Colchicine (CLC) and Pitstop‐2 (all Sigma) were dissolved in dimethylsulfoxide (DMSO) or TAP, respectively.3‐Isobutyl‐1‐methylxanthine (IBMX), 4‐hydroxy‐2,2,6,6‐tetramethyl‐piperidine 1‐oxyl (TEMPOL), Colchicine (CLC) and Pitstop‐2 (all Sigma) were dissolved in dimethylsulfoxide (DMSO) or TAP, respectively.. CK‐666, CK‐689 (Merck), Flow Cytometry:Article Title: SUMOylation-dependent degradation of nucleocapsid is responsible for Pestivirus uncoating Article Snippet: Porcine kidney (PK-15) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO) supplemented with 10% fetal bovine serum (FBS) (GIBCO, Invitrogen), 100 mg/mL streptomycin, and 100 IU/mL penicillin (GIBCO, Invitrogen), and maintained at 37°C in a humidified atmosphere with 5% CO 2 .Porcine kidney (PK-15) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO) supplemented with 10% fetal bovine serum (FBS) (GIBCO, Invitrogen), 100 mg/mL streptomycin, and 100 IU/mL penicillin (GIBCO, Invitrogen), and maintained at 37°C in a humidified atmosphere with 5% CO 2 .. The compounds MG132, Rapamycin, BafA1, Bortezomib, Pyr-41, and Article Title: SUMOylation-dependent degradation of nucleocapsid is responsible for Pestivirus uncoating. Article Snippet: Porcine kidney (PK-15) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO) supplemented with 10% fetal bovine serum (FBS) (GIBCO, Invitrogen), 100 mg/mL streptomycin, and 100 IU/mL penicillin (GIBCO, Invitrogen), and maintained at 37°C in a humidified atmosphere with 5% CO2.Porcine kidney (PK-15) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO) supplemented with 10% fetal bovine serum (FBS) (GIBCO, Invitrogen), 100 mg/mL streptomycin, and 100 IU/mL penicillin (GIBCO, Invitrogen), and maintained at 37°C in a humidified atmosphere with 5% CO2.. The compounds MG132, Rapamycin, BafA1, Bortezomib, Pyr-41, and Article Title: Nanomaterial signatures program biomolecular condensates via triphasic separation for chemoplasticity remodeling. Article Snippet: China).. TAK-243, Spautin-1, Myricetin, Grp94, AMP-PCP, CB5083, DBeQ, Article Title: Nanomaterial signatures program biomolecular condensates via triphasic separation for chemoplasticity remodeling Article Snippet: TAK-243, Spautin-1, Myricetin, Grp94, AMP-PCP, CB5083, DBeQ, Article Title: Hypoxia Exacerbates Kir2.1 Channel Dysfunction in an Andersen-Tawil Syndrome Variant Through a SUMO-Dependent Mechanism Article Snippet: The monoclonal antibody against Kir2.1 (RRID: AB_11000720) was purchased from Neuromab and the polyclonal antibody for SUMO1 [Y299] (ab32058) was from Abcam.The monoclonal antibody against Kir2.1 (RRID: AB_11000720) was purchased from Neuromab and the polyclonal antibody for SUMO1 [Y299] (ab32058) was from Abcam.. TAK-981 was from Article Title: Comprehensive SUMO Proteomic Analyses Identify HIV Latency-Associated Proteins in Microglia. Article Snippet: WB, western blot; IF, immunofluorescence.WB, western blot; IF, immunofluorescence.. The SUMO inhibitors TAK-981 and Article Title: Comprehensive SUMO Proteomic Analyses Identify HIV Latency-Associated Proteins in Microglia Article Snippet: western blot; IF, immunofluorescence. .. The SUMO inhibitors TAK-981 and Article Title: Insights into degradation and targeting of the photoreceptor channelrhodopsin-1. Article Snippet: 3‐Isobutyl‐1‐methylxanthine (IBMX), 4‐hydroxy‐2,2,6,6‐tetramethyl‐piperidine 1‐oxyl (TEMPOL), Colchicine (CLC) and Pitstop‐2 (all Sigma) were dissolved in dimethylsulfoxide (DMSO) or TAP, respectively.3‐Isobutyl‐1‐methylxanthine (IBMX), 4‐hydroxy‐2,2,6,6‐tetramethyl‐piperidine 1‐oxyl (TEMPOL), Colchicine (CLC) and Pitstop‐2 (all Sigma) were dissolved in dimethylsulfoxide (DMSO) or TAP, respectively.. CK‐666, CK‐689 (Merck), Incubation:Article Title: SUMOylation-dependent degradation of nucleocapsid is responsible for Pestivirus uncoating Article Snippet: Porcine kidney (PK-15) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO) supplemented with 10% fetal bovine serum (FBS) (GIBCO, Invitrogen), 100 mg/mL streptomycin, and 100 IU/mL penicillin (GIBCO, Invitrogen), and maintained at 37°C in a humidified atmosphere with 5% CO 2 .Porcine kidney (PK-15) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO) supplemented with 10% fetal bovine serum (FBS) (GIBCO, Invitrogen), 100 mg/mL streptomycin, and 100 IU/mL penicillin (GIBCO, Invitrogen), and maintained at 37°C in a humidified atmosphere with 5% CO 2 .. The compounds MG132, Rapamycin, BafA1, Bortezomib, Pyr-41, and Article Title: SUMOylation-dependent degradation of nucleocapsid is responsible for Pestivirus uncoating. Article Snippet: Porcine kidney (PK-15) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO) supplemented with 10% fetal bovine serum (FBS) (GIBCO, Invitrogen), 100 mg/mL streptomycin, and 100 IU/mL penicillin (GIBCO, Invitrogen), and maintained at 37°C in a humidified atmosphere with 5% CO2.Porcine kidney (PK-15) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO) supplemented with 10% fetal bovine serum (FBS) (GIBCO, Invitrogen), 100 mg/mL streptomycin, and 100 IU/mL penicillin (GIBCO, Invitrogen), and maintained at 37°C in a humidified atmosphere with 5% CO2.. The compounds MG132, Rapamycin, BafA1, Bortezomib, Pyr-41, and Article Title: Nanomaterial signatures program biomolecular condensates via triphasic separation for chemoplasticity remodeling. Article Snippet: China).. TAK-243, Spautin-1, Myricetin, Grp94, AMP-PCP, CB5083, DBeQ, Article Title: Nanomaterial signatures program biomolecular condensates via triphasic separation for chemoplasticity remodeling Article Snippet: TAK-243, Spautin-1, Myricetin, Grp94, AMP-PCP, CB5083, DBeQ, Article Title: Hypoxia Exacerbates Kir2.1 Channel Dysfunction in an Andersen-Tawil Syndrome Variant Through a SUMO-Dependent Mechanism Article Snippet: The monoclonal antibody against Kir2.1 (RRID: AB_11000720) was purchased from Neuromab and the polyclonal antibody for SUMO1 [Y299] (ab32058) was from Abcam.The monoclonal antibody against Kir2.1 (RRID: AB_11000720) was purchased from Neuromab and the polyclonal antibody for SUMO1 [Y299] (ab32058) was from Abcam.. TAK-981 was from Article Title: Comprehensive SUMO Proteomic Analyses Identify HIV Latency-Associated Proteins in Microglia. Article Snippet: WB, western blot; IF, immunofluorescence.WB, western blot; IF, immunofluorescence.. The SUMO inhibitors TAK-981 and Article Title: Comprehensive SUMO Proteomic Analyses Identify HIV Latency-Associated Proteins in Microglia Article Snippet: western blot; IF, immunofluorescence. .. The SUMO inhibitors TAK-981 and Article Title: Insights into degradation and targeting of the photoreceptor channelrhodopsin-1. Article Snippet: 3‐Isobutyl‐1‐methylxanthine (IBMX), 4‐hydroxy‐2,2,6,6‐tetramethyl‐piperidine 1‐oxyl (TEMPOL), Colchicine (CLC) and Pitstop‐2 (all Sigma) were dissolved in dimethylsulfoxide (DMSO) or TAP, respectively.3‐Isobutyl‐1‐methylxanthine (IBMX), 4‐hydroxy‐2,2,6,6‐tetramethyl‐piperidine 1‐oxyl (TEMPOL), Colchicine (CLC) and Pitstop‐2 (all Sigma) were dissolved in dimethylsulfoxide (DMSO) or TAP, respectively.. CK‐666, CK‐689 (Merck), Solvent:Article Title: SUMOylation-dependent degradation of nucleocapsid is responsible for Pestivirus uncoating Article Snippet: Porcine kidney (PK-15) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO) supplemented with 10% fetal bovine serum (FBS) (GIBCO, Invitrogen), 100 mg/mL streptomycin, and 100 IU/mL penicillin (GIBCO, Invitrogen), and maintained at 37°C in a humidified atmosphere with 5% CO 2 .Porcine kidney (PK-15) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO) supplemented with 10% fetal bovine serum (FBS) (GIBCO, Invitrogen), 100 mg/mL streptomycin, and 100 IU/mL penicillin (GIBCO, Invitrogen), and maintained at 37°C in a humidified atmosphere with 5% CO 2 .. The compounds MG132, Rapamycin, BafA1, Bortezomib, Pyr-41, and Article Title: SUMOylation-dependent degradation of nucleocapsid is responsible for Pestivirus uncoating. Article Snippet: Porcine kidney (PK-15) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO) supplemented with 10% fetal bovine serum (FBS) (GIBCO, Invitrogen), 100 mg/mL streptomycin, and 100 IU/mL penicillin (GIBCO, Invitrogen), and maintained at 37°C in a humidified atmosphere with 5% CO2.Porcine kidney (PK-15) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO) supplemented with 10% fetal bovine serum (FBS) (GIBCO, Invitrogen), 100 mg/mL streptomycin, and 100 IU/mL penicillin (GIBCO, Invitrogen), and maintained at 37°C in a humidified atmosphere with 5% CO2.. The compounds MG132, Rapamycin, BafA1, Bortezomib, Pyr-41, and Article Title: Nanomaterial signatures program biomolecular condensates via triphasic separation for chemoplasticity remodeling. Article Snippet: China).. TAK-243, Spautin-1, Myricetin, Grp94, AMP-PCP, CB5083, DBeQ, Article Title: Nanomaterial signatures program biomolecular condensates via triphasic separation for chemoplasticity remodeling Article Snippet: TAK-243, Spautin-1, Myricetin, Grp94, AMP-PCP, CB5083, DBeQ, Article Title: Hypoxia Exacerbates Kir2.1 Channel Dysfunction in an Andersen-Tawil Syndrome Variant Through a SUMO-Dependent Mechanism Article Snippet: The monoclonal antibody against Kir2.1 (RRID: AB_11000720) was purchased from Neuromab and the polyclonal antibody for SUMO1 [Y299] (ab32058) was from Abcam.The monoclonal antibody against Kir2.1 (RRID: AB_11000720) was purchased from Neuromab and the polyclonal antibody for SUMO1 [Y299] (ab32058) was from Abcam.. TAK-981 was from Article Title: Comprehensive SUMO Proteomic Analyses Identify HIV Latency-Associated Proteins in Microglia. Article Snippet: WB, western blot; IF, immunofluorescence.WB, western blot; IF, immunofluorescence.. The SUMO inhibitors TAK-981 and Article Title: Comprehensive SUMO Proteomic Analyses Identify HIV Latency-Associated Proteins in Microglia Article Snippet: western blot; IF, immunofluorescence. .. The SUMO inhibitors TAK-981 and Article Title: Insights into degradation and targeting of the photoreceptor channelrhodopsin-1. Article Snippet: 3‐Isobutyl‐1‐methylxanthine (IBMX), 4‐hydroxy‐2,2,6,6‐tetramethyl‐piperidine 1‐oxyl (TEMPOL), Colchicine (CLC) and Pitstop‐2 (all Sigma) were dissolved in dimethylsulfoxide (DMSO) or TAP, respectively.3‐Isobutyl‐1‐methylxanthine (IBMX), 4‐hydroxy‐2,2,6,6‐tetramethyl‐piperidine 1‐oxyl (TEMPOL), Colchicine (CLC) and Pitstop‐2 (all Sigma) were dissolved in dimethylsulfoxide (DMSO) or TAP, respectively.. CK‐666, CK‐689 (Merck), |
