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Structured Review

Proteintech cyc1
Cyc1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cyc1/CYC1+Antibody/pm42849801-69-20-21
Average 93 stars, based on 34 article reviews
cyc1 - by Bioz Stars, 2026-11
93/100 stars

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Related Articles

Ubiquitin Proteomics:

Article Title: UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation
Article Snippet: .. The rabbit UBXN1 (16135-1-AP 1:7000 dilution), p97 (10736-1-AP), BAG6 (26417-1-AP), ATF6 (24169-1-AP), Sec61β (51020-2-AP 1:3000 dilution), TIMM17A (11189-1-AP), Aconitase 2 (11134-1-AP), calnexin (10427-2-AP), CYC1 (10242-1-AP), and G3BP1 (13057-2-AP) antibodies were obtained from Proteintech Inc; rabbit BiP (3177 dilution), peIF2α (3398 1:500 dilution), and MCM3 (4012S) antibodies were obtained from Cell Signaling Technology; mouse PCNA (sc-56 1:3000 dilution), ATF4 (sc-390063 1:500 dilution), TOMM20 (sc-17764), TOMM70 (sc-390545), TIMM23 (sc-514463), ubiquitin (P4D1; sc8017), β-actin (sc-69879 1:3000 dilution), GAPDH (sc-47724 1:3000 dilution), c-myc (sc-40 1:3000 dilution), Sec61α (sc-393182 1:500 dilution), and TRAPα (sc-373916) were from Santa Cruz Biotechnology; mouse ubiquitin FK2 (04-263 1:100 dilution) for immunofluorescence and Anti-Puromycin, clone 12D10 (MABE343 1:3000 dilution) was from EMD Millipore; mouse FLAG-M2 antibody (F3165 1:1000 dilution) was from Sigma Aldrich. .. The rabbit anti-Prion protein PrP antibody (ab52604) was purchased from Abcam.

Article Title: UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation.
Article Snippet: .. The rabbit UBXN1 (16135-1-AP 1:7000 dilution), p97 (10736-1- AP), BAG6 (26417-1-AP), ATF6 (24169-1-AP), Sec61β (51020-2- AP 1:3000 dilution), TIMM17A (11189-1-AP), Aconitase 2 (11134- 1-AP), calnexin (10427-2-AP), CYC1 (10242-1-AP), and G3BP1 (13057-2-AP) antibodies were obtained from Proteintech Inc; rabbit BiP (3177 dilution), peIF2α (3398 1:500 dilution), and MCM3 (4012S) antibodies were obtained from Cell Signaling Technology; mouse PCNA (sc-56 1:3000 dilution), ATF4 (sc390063 1:500 dilution), TOMM20 (sc-17764), TOMM70 (sc390545), TIMM23 (sc-514463), ubiquitin (P4D1; sc8017), β-actin (sc-69879 1:3000 dilution), GAPDH (sc-47724 1:3000 dilution), c-myc (sc-40 1:3000 dilution), Sec61α (sc-393182 1:500 dilution), and TRAPα (sc-373916) were from Santa Cruz Biotechnology; mouse ubiquitin FK2 (04-263 1:100 dilution) for immunofluorescence and Anti-Puromycin, clone 12D10 (MABE343 1:3000 dilution) was from EMD Millipore; mouse FLAG-M2 antibody (F3165 1:1000 dilution) was from Sigma Aldrich. .. The rabbit antiPrion protein PrP antibody (ab52604) was purchased from Abcam.

Immunofluorescence:

Article Title: UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation
Article Snippet: .. The rabbit UBXN1 (16135-1-AP 1:7000 dilution), p97 (10736-1-AP), BAG6 (26417-1-AP), ATF6 (24169-1-AP), Sec61β (51020-2-AP 1:3000 dilution), TIMM17A (11189-1-AP), Aconitase 2 (11134-1-AP), calnexin (10427-2-AP), CYC1 (10242-1-AP), and G3BP1 (13057-2-AP) antibodies were obtained from Proteintech Inc; rabbit BiP (3177 dilution), peIF2α (3398 1:500 dilution), and MCM3 (4012S) antibodies were obtained from Cell Signaling Technology; mouse PCNA (sc-56 1:3000 dilution), ATF4 (sc-390063 1:500 dilution), TOMM20 (sc-17764), TOMM70 (sc-390545), TIMM23 (sc-514463), ubiquitin (P4D1; sc8017), β-actin (sc-69879 1:3000 dilution), GAPDH (sc-47724 1:3000 dilution), c-myc (sc-40 1:3000 dilution), Sec61α (sc-393182 1:500 dilution), and TRAPα (sc-373916) were from Santa Cruz Biotechnology; mouse ubiquitin FK2 (04-263 1:100 dilution) for immunofluorescence and Anti-Puromycin, clone 12D10 (MABE343 1:3000 dilution) was from EMD Millipore; mouse FLAG-M2 antibody (F3165 1:1000 dilution) was from Sigma Aldrich. .. The rabbit anti-Prion protein PrP antibody (ab52604) was purchased from Abcam.

Article Title: UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation.
Article Snippet: .. The rabbit UBXN1 (16135-1-AP 1:7000 dilution), p97 (10736-1- AP), BAG6 (26417-1-AP), ATF6 (24169-1-AP), Sec61β (51020-2- AP 1:3000 dilution), TIMM17A (11189-1-AP), Aconitase 2 (11134- 1-AP), calnexin (10427-2-AP), CYC1 (10242-1-AP), and G3BP1 (13057-2-AP) antibodies were obtained from Proteintech Inc; rabbit BiP (3177 dilution), peIF2α (3398 1:500 dilution), and MCM3 (4012S) antibodies were obtained from Cell Signaling Technology; mouse PCNA (sc-56 1:3000 dilution), ATF4 (sc390063 1:500 dilution), TOMM20 (sc-17764), TOMM70 (sc390545), TIMM23 (sc-514463), ubiquitin (P4D1; sc8017), β-actin (sc-69879 1:3000 dilution), GAPDH (sc-47724 1:3000 dilution), c-myc (sc-40 1:3000 dilution), Sec61α (sc-393182 1:500 dilution), and TRAPα (sc-373916) were from Santa Cruz Biotechnology; mouse ubiquitin FK2 (04-263 1:100 dilution) for immunofluorescence and Anti-Puromycin, clone 12D10 (MABE343 1:3000 dilution) was from EMD Millipore; mouse FLAG-M2 antibody (F3165 1:1000 dilution) was from Sigma Aldrich. .. The rabbit antiPrion protein PrP antibody (ab52604) was purchased from Abcam.

Membrane:

Article Title: Rhodoquinone carries electrons in the mammalian electron transport chain.
Article Snippet: Mitochondrial lysates were centrifuged at 20000 g for 20 min at 4 C, the supernatant was mixed with NativePAGE G-250 sample additive (0.25 % detergent concentration) loaded onto NativePAGE 3-12 %, Bis-Tris gels (Thermo BN1001) and run according to manufacturer’s instructions (Thermo BN2007). .. Proteins were transferred onto a 0.45 mm PVDF (Sigma) membrane using 1x NuPAGE transfer buffer (Thermo NP0006), and subsequently probed for NDUFS1 (Cell Signaling 60153S), SDHA (ProteinTech 14865-1-AP), CYC1 (ProteinTech 10242-1-AP), COX4 (Cell Signaling 4850S) and ATP5A1 (Cell Signaling 18023S). ..

Incubation:

Article Title: CYC1 silencing exacerbates hypoxia-induced neuronal death via mitochondrial apoptosis under hyperglycemia.
Article Snippet: Cytochrome c1 (CYC1) is a subunit of mitochondrial complex III, which is known to play a key role in ischemic brain injury.. However, the functional role of CYC1 in hyperglycemic cerebral ischemia/reperfusion remains unclear.. In this study, we investigated the effect of CYC1 gene knockdown in an in vitro model mimicking hyperglycemic ischemia/reperfusion.

Saline:

Article Title: CYC1 silencing exacerbates hypoxia-induced neuronal death via mitochondrial apoptosis under hyperglycemia.
Article Snippet: Cytochrome c1 (CYC1) is a subunit of mitochondrial complex III, which is known to play a key role in ischemic brain injury.. However, the functional role of CYC1 in hyperglycemic cerebral ischemia/reperfusion remains unclear.. In this study, we investigated the effect of CYC1 gene knockdown in an in vitro model mimicking hyperglycemic ischemia/reperfusion.

Cell-Signaling:

Article Title: CYC1 silencing exacerbates hypoxia-induced neuronal death via mitochondrial apoptosis under hyperglycemia.
Article Snippet: Cytochrome c1 (CYC1) is a subunit of mitochondrial complex III, which is known to play a key role in ischemic brain injury.. However, the functional role of CYC1 in hyperglycemic cerebral ischemia/reperfusion remains unclear.. In this study, we investigated the effect of CYC1 gene knockdown in an in vitro model mimicking hyperglycemic ischemia/reperfusion.



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