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cxadr (Proteintech)

Name: cxadr
Brand: Proteintech
Rating: 93 (7 reviews)

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Proteintech cxadr

GC protein is a key factor involved in the PNI of PDAC. A) Representative H&E images showed the nerves in PNI‐low and PNI‐high PDAC samples; the asterisk indicates nerves, arrow represent cancer cells, scale bar, 100 µm. B) Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation of differentially expressed genes (DEGs) between PNI‐low and PNI‐high group. C) Venn diagram showed the filter criteria of the candidate DEGs between the PNI‐low and PNI‐high groups. D) Single cell RNA sequencing analysis showed the expression pattern of CRISP3, SCGB3A1, CGB5, <t>CXADR,</t> CST6, <t>DEFB1,</t> <t>TCN1,</t> PCSK2, SERPINA4, CFC1, GC, SERPINA5 , and SPINK1 in human PDAC tissues. Data were obtained from CRA001160 and GSE202051 . E) Representative immunohistochemical staining of CRISP3, TCN1, SCGB3A1, CXADR, and GC protein expression level in human PDAC tissues in the Ren Ji cohort, scale bar, 100 µm. F) The association between CRISP3, TCN1, SCGB3A1, CXADR, and GC expression level and PNI degree in PDAC tissue in the Ren Ji cohort (n = 134). The yellow, cyan, turquoise, and purple represented ‐, +, ++, and +++ expression level of indicated proteins, respectively. G) Representative immunohistochemical staining of GC protein in human PDAC tissues from the Ren Ji cohort. The asterisk indicates nerves, and arrow represents cancer cells; scale bar, 100 µm. H) Immunofluorescence analysis showed the expression pattern of GC protein in the PNI‐low and PNI‐high human PDAC tissues. Scale bar, 50 µm. **** P < 0.0001.

Cxadr, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cxadr/product/Proteintech
Average 93 stars, based on 7 article reviews

cxadr - by Bioz Stars, 2026-02

93/100 stars

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1) Product Images from "Vitamin D Binding Protein, a Ligand of Integrin beta 1, Motivates Both Tumor Cells and Schwann Cells to Promote Perineural Invasion in Pancreatic Ductal Adenocarcinoma"

Article Title: Vitamin D Binding Protein, a Ligand of Integrin beta 1, Motivates Both Tumor Cells and Schwann Cells to Promote Perineural Invasion in Pancreatic Ductal Adenocarcinoma

Journal: Advanced Science

doi: 10.1002/advs.202511726

Figure Legend Snippet: GC protein is a key factor involved in the PNI of PDAC. A) Representative H&E images showed the nerves in PNI‐low and PNI‐high PDAC samples; the asterisk indicates nerves, arrow represent cancer cells, scale bar, 100 µm. B) Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation of differentially expressed genes (DEGs) between PNI‐low and PNI‐high group. C) Venn diagram showed the filter criteria of the candidate DEGs between the PNI‐low and PNI‐high groups. D) Single cell RNA sequencing analysis showed the expression pattern of CRISP3, SCGB3A1, CGB5, CXADR, CST6, DEFB1, TCN1, PCSK2, SERPINA4, CFC1, GC, SERPINA5 , and SPINK1 in human PDAC tissues. Data were obtained from CRA001160 and GSE202051 . E) Representative immunohistochemical staining of CRISP3, TCN1, SCGB3A1, CXADR, and GC protein expression level in human PDAC tissues in the Ren Ji cohort, scale bar, 100 µm. F) The association between CRISP3, TCN1, SCGB3A1, CXADR, and GC expression level and PNI degree in PDAC tissue in the Ren Ji cohort (n = 134). The yellow, cyan, turquoise, and purple represented ‐, +, ++, and +++ expression level of indicated proteins, respectively. G) Representative immunohistochemical staining of GC protein in human PDAC tissues from the Ren Ji cohort. The asterisk indicates nerves, and arrow represents cancer cells; scale bar, 100 µm. H) Immunofluorescence analysis showed the expression pattern of GC protein in the PNI‐low and PNI‐high human PDAC tissues. Scale bar, 50 µm. **** P < 0.0001.

Techniques Used: RNA Sequencing, Expressing, Immunohistochemical staining, Staining, Immunofluorescence

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Journal: Advanced Science

Article Title: Vitamin D Binding Protein, a Ligand of Integrin beta 1, Motivates Both Tumor Cells and Schwann Cells to Promote Perineural Invasion in Pancreatic Ductal Adenocarcinoma

doi: 10.1002/advs.202511726

Figure Lengend Snippet: GC protein is a key factor involved in the PNI of PDAC. A) Representative H&E images showed the nerves in PNI‐low and PNI‐high PDAC samples; the asterisk indicates nerves, arrow represent cancer cells, scale bar, 100 µm. B) Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation of differentially expressed genes (DEGs) between PNI‐low and PNI‐high group. C) Venn diagram showed the filter criteria of the candidate DEGs between the PNI‐low and PNI‐high groups. D) Single cell RNA sequencing analysis showed the expression pattern of CRISP3, SCGB3A1, CGB5, CXADR, CST6, DEFB1, TCN1, PCSK2, SERPINA4, CFC1, GC, SERPINA5 , and SPINK1 in human PDAC tissues. Data were obtained from CRA001160 and GSE202051 . E) Representative immunohistochemical staining of CRISP3, TCN1, SCGB3A1, CXADR, and GC protein expression level in human PDAC tissues in the Ren Ji cohort, scale bar, 100 µm. F) The association between CRISP3, TCN1, SCGB3A1, CXADR, and GC expression level and PNI degree in PDAC tissue in the Ren Ji cohort (n = 134). The yellow, cyan, turquoise, and purple represented ‐, +, ++, and +++ expression level of indicated proteins, respectively. G) Representative immunohistochemical staining of GC protein in human PDAC tissues from the Ren Ji cohort. The asterisk indicates nerves, and arrow represents cancer cells; scale bar, 100 µm. H) Immunofluorescence analysis showed the expression pattern of GC protein in the PNI‐low and PNI‐high human PDAC tissues. Scale bar, 50 µm. **** P < 0.0001.

Article Snippet: The primary antibodies were listed as follows: GC (1:100, Proteintech, 16922‐1‐AP, RRID: AB_10597098), ITGB1 (1:100, Proteintech, 12594‐1‐AP, RRID: AB_2130085), FAK (1:1000, Cell Signaling Technology, 71433, RRID: AB_2799801), p‐FAK (Tyr397) (1:50, Cell Signaling Technology, 8556, RRID: AB_10891442), PGP9.5 (1:4000, Proteintech, 66230‐1‐Ig, RRID: AB_2881621), CRISP3 (1:500, Proteintech, 14847‐1‐AP, RRID: AB_1607510), TCN1 (1:100, Proteintech, 16078‐1‐AP, RRID: AB_2878215), SCGB3A1 (1:100, Novus, NBP1‐36983, RRID: AB_2183538), CXADR (1:100, Proteintech, 11777‐1‐AP, RRID: AB_2087443), and HPR‐link secondary antibodies (anti‐Rb, 1:5000, ab205718, RRID: AB_2819160, anti‐Ms, 1:5000, ab205719, RRID: AB_2755049, Abcam, USA).

Techniques: RNA Sequencing, Expressing, Immunohistochemical staining, Staining, Immunofluorescence

The cell surface CAR- or HBGA-positive cell population did not increase after two rounds of sorting and expansion or incubating cells in human AB serum, respectively. ( A ) Live cell staining of the original or sorted and expanded CAR-positive cell populations with anti-CAR-FITC antibody. No enrichment of CAR-positive cells was achieved. ( B ) Live cell staining of the original (unsoaked) and human AB serum-soaked BJAB cell populations with anti-B mouse Mab and AF488-labeled secondary antibody. Soaking BJAB cells in human AB serum did not increase the number of HBGA-positive cells. One representative of three independent experiments with the BJAB-LSU cell clone is shown.

Journal: Journal of Virology

Article Title: B cell lines fail to support efficient rhesus enteric calicivirus and human norovirus replication

doi: 10.1128/jvi.00143-25

Figure Lengend Snippet: The cell surface CAR- or HBGA-positive cell population did not increase after two rounds of sorting and expansion or incubating cells in human AB serum, respectively. ( A ) Live cell staining of the original or sorted and expanded CAR-positive cell populations with anti-CAR-FITC antibody. No enrichment of CAR-positive cells was achieved. ( B ) Live cell staining of the original (unsoaked) and human AB serum-soaked BJAB cell populations with anti-B mouse Mab and AF488-labeled secondary antibody. Soaking BJAB cells in human AB serum did not increase the number of HBGA-positive cells. One representative of three independent experiments with the BJAB-LSU cell clone is shown.

Article Snippet: Recombinant anti-CXADR/CAR antibody (FITC) (SinoBiological, Cat# 10799-R271-F) and anti-A (ABO1) and anti-B (ABO2) (Diagast, Cat# 70501 and 70502) mouse monoclonal antibodies (IgM) were used for CAR and type A or type B HBGA staining, respectively.

Techniques: Staining, Labeling

Cell surface expression of CAR and the type A HBGA in rBJAB-CAR+/A + cells. ( A ) Nonpermeabilized BJAB and rBJAB-CAR+/A + cells were stained by the anti-CAR antibody (RmcB) and AF-594 secondary antibody. Cell surface expression of CAR (white arrows) could be detected only in the rBJAB-CAR+/A + cultures. ( B ) Live cell staining with anti-CAR-FITC antibody and flow cytometry analysis revealed cell surface CAR on ~99% of the rBJAB-CAR+/A + cells, while only ~1% of the parental BJAB cells stained positive. ( C ) Live cell staining and flow cytometry analysis of the rBJAB-CAR+/A + cell population with anti-A mouse Mab- and AF488-labeled secondary antibody revealed the presence of cell surface type A HBGA in ~65% of the cells. One of three individual experiments with BJAB-LSU is shown. (D) BJAB and rBJAB CAR +A + cells were infected with ReCV-FT285 (1 MOI), and infectious virus titers were evaluated by titration on LLC-MK2 monolayers at 0 hpi and 72 hpi. The mean and SD of three independent experiments are shown.

Journal: Journal of Virology

Article Title: B cell lines fail to support efficient rhesus enteric calicivirus and human norovirus replication

doi: 10.1128/jvi.00143-25

Figure Lengend Snippet: Cell surface expression of CAR and the type A HBGA in rBJAB-CAR+/A + cells. ( A ) Nonpermeabilized BJAB and rBJAB-CAR+/A + cells were stained by the anti-CAR antibody (RmcB) and AF-594 secondary antibody. Cell surface expression of CAR (white arrows) could be detected only in the rBJAB-CAR+/A + cultures. ( B ) Live cell staining with anti-CAR-FITC antibody and flow cytometry analysis revealed cell surface CAR on ~99% of the rBJAB-CAR+/A + cells, while only ~1% of the parental BJAB cells stained positive. ( C ) Live cell staining and flow cytometry analysis of the rBJAB-CAR+/A + cell population with anti-A mouse Mab- and AF488-labeled secondary antibody revealed the presence of cell surface type A HBGA in ~65% of the cells. One of three individual experiments with BJAB-LSU is shown. (D) BJAB and rBJAB CAR +A + cells were infected with ReCV-FT285 (1 MOI), and infectious virus titers were evaluated by titration on LLC-MK2 monolayers at 0 hpi and 72 hpi. The mean and SD of three independent experiments are shown.

Techniques: Expressing, Staining, Flow Cytometry, Labeling, Infection, Virus, Titration

a UMAP plots of the Inhibit dataset of genes enriched in the presumptive JZP cluster. Phlda2 is a well-known marker of the JZ. Yet SynT-I markers Slc16a1 and Hbegf are enriched in the same or adjacent cluster (arrow), as is Cxadr . Colour gradient shows normalised expression. b UMAP plots of locations of cells with top 10% expression of Phlda2 , Slc16a1 and overlap between the two cell populations. c Immunostaining for SynT-I marker STRA6 (green) and junctional zone marker NCAM1 (red) reveals non-overlap between individual LP and JZP cells entering these lineages. Staining was carried out after 48 h of culture in Inhibit conditions. Image is representative of three independent experiments. d Diagram depicting the organization of emerging trophoblast cell types at sites of fetal vessel invagination into the chorionic ectoderm. JZP = junctional zone precursor, mbs = maternal blood space, sTGC =sinusoidal trophoblast giant cell, LP = labyrinth precursor. e Cxadr expression dynamics across the 48 h differentiation time course in Inhibit and Remove conditions. During these early stages of differentiation, Cxadr expression is up-regulated, in particular in Inhibit conditions that promote LP differentiation. Data are normalized to stem cell conditions and plotted as mean +/- SEM of three independent replicates. Statistical significance was calculated using 2-way ANOVA. **** p < 0.0001. Source data are provided as a Source Data file which contains exact P values. f Representative image of differentiated TSCs at 3 days (3D) of differentiation stained for CXADR (green) and cell membrane marker ZO1 (red). The encircled area highlights early-stage syncytializing cells that have lost CXADR while still retaining some ZO1. Image is representative of three independent experiments. g E10.5 placenta stained for CXADR (green) and MCT1 (red) showing the immediately adjacent localisation of CXADR-positive single cells to syncytial MCT1-positive cells. Images are representative of n = 4 placentas. The middle column of images depicts a higher magnification of the boxed area in the left column. The right-hand column of images depicts an area at the base of the placental labyrinth taken from a consecutive section of that shown on the left. Images are representative of three independent samples.

Journal: Nature Communications

Article Title: Single-cell RNA sequencing identifies CXADR as a fate determinant of the placental exchange surface

doi: 10.1038/s41467-024-55597-w

Figure Lengend Snippet: a UMAP plots of the Inhibit dataset of genes enriched in the presumptive JZP cluster. Phlda2 is a well-known marker of the JZ. Yet SynT-I markers Slc16a1 and Hbegf are enriched in the same or adjacent cluster (arrow), as is Cxadr . Colour gradient shows normalised expression. b UMAP plots of locations of cells with top 10% expression of Phlda2 , Slc16a1 and overlap between the two cell populations. c Immunostaining for SynT-I marker STRA6 (green) and junctional zone marker NCAM1 (red) reveals non-overlap between individual LP and JZP cells entering these lineages. Staining was carried out after 48 h of culture in Inhibit conditions. Image is representative of three independent experiments. d Diagram depicting the organization of emerging trophoblast cell types at sites of fetal vessel invagination into the chorionic ectoderm. JZP = junctional zone precursor, mbs = maternal blood space, sTGC =sinusoidal trophoblast giant cell, LP = labyrinth precursor. e Cxadr expression dynamics across the 48 h differentiation time course in Inhibit and Remove conditions. During these early stages of differentiation, Cxadr expression is up-regulated, in particular in Inhibit conditions that promote LP differentiation. Data are normalized to stem cell conditions and plotted as mean +/- SEM of three independent replicates. Statistical significance was calculated using 2-way ANOVA. **** p < 0.0001. Source data are provided as a Source Data file which contains exact P values. f Representative image of differentiated TSCs at 3 days (3D) of differentiation stained for CXADR (green) and cell membrane marker ZO1 (red). The encircled area highlights early-stage syncytializing cells that have lost CXADR while still retaining some ZO1. Image is representative of three independent experiments. g E10.5 placenta stained for CXADR (green) and MCT1 (red) showing the immediately adjacent localisation of CXADR-positive single cells to syncytial MCT1-positive cells. Images are representative of n = 4 placentas. The middle column of images depicts a higher magnification of the boxed area in the left column. The right-hand column of images depicts an area at the base of the placental labyrinth taken from a consecutive section of that shown on the left. Images are representative of three independent samples.

Article Snippet: Primary antibodies and dilutions used were: CXADR 1:100 (R&D Systems AF2654), MCT1 1:100 (EMD Millipore AB1286-I), MCT4 1:100 (EMD Millipore AB3314P), Embigin 1:100 (ThermoFisher Scientific 12-5839-82), ZO1 1:200 (ThermoFisher Scientific 339100), NCAM1 1:100 (R&D AF2408-SP), STRA6 1:100 (Novus Biologicals NBP3-12353), SOX2 1:200 (R&D AF2018), KRT18 1:100 (Research Diagnostics RDI-PRO 61028), and NICOL1 1:100 (St Johns STJ196219).

Techniques: Marker, Expressing, Immunostaining, Staining, Membrane

a Schematic depicting the generation of Cxadr -null TSCs by CRISPR-Cas9 targeting of exon 2. b Immunofluorescence staining of wild-type (WT) and knockout (KO) TSCs against CXADR, showing cell membrane localisation in WT cells, and lack of positive staining in KO TSCs. Images are representative of n = 5 WT independently derived clones and n = 3 independently derived KO clones. c Diagram depicting experimental set-up, where WT and KO TSCs were used for RT-qPCR and immunofluorescence staining across a differentiation time course. d Proliferation rates of WT (n = 3 clones) and KO ( n = 3 clones) TSCs in differentiation conditions (Remove) over a 4-day period. No differences were observed. Statistical analysis was performed by two-sided unpaired t-test per day. Data are displayed as mean +/- SEM. e Immunofluorescence staining of WT and KO TSCs after 4 days of differentiation against cell membrane marker ZO1, depicting the presence of a smooth continuous ZO1-stained cell border in non-syncytial, single cells in the WT clone, and complete syncytialisation in the KO clone. Images are representative of n = 4 WT clones and n = 3 KO clones. f Bar chart showing the significant increase in syncytialized cells in KO ( n = 3 clones) compared to WT TSCs at 3 days (3D) and 4 days (4D) of differentiation (WT n = 5 TSC clones at 3D and n = 4 at 4D). Statistical significance was calculated using two-sided unpaired t-test; data are plotted as mean +/- SEM. *** * p < 0.0001. g RT-qPCR analysis of wild-type (WT, n = 5 clones) and Cxadr knockout (KO, n = 3 clones) TSC clones grown in stem cell conditions and after 2-, 3-, and 4-days of differentiation in Remove conditions. Genes assessed included TSC markers ( Cdx2 , Eomes ), and markers of the syncytiotrophoblast layers II ( = SynT-II; Gcm1 , Synb , Gabrp ) and SynT-I ( Syna , Slc16a / Mct1 , Atp11a , Hbegf ). Data are normalized to WT TSCs in stem cell conditions and plotted as mean +/- SEM. Statistical significance was calculated using 2-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Source data and full P values for d, f, and g are provided as a Source Data file.

Journal: Nature Communications

Article Title: Single-cell RNA sequencing identifies CXADR as a fate determinant of the placental exchange surface

doi: 10.1038/s41467-024-55597-w

Figure Lengend Snippet: a Schematic depicting the generation of Cxadr -null TSCs by CRISPR-Cas9 targeting of exon 2. b Immunofluorescence staining of wild-type (WT) and knockout (KO) TSCs against CXADR, showing cell membrane localisation in WT cells, and lack of positive staining in KO TSCs. Images are representative of n = 5 WT independently derived clones and n = 3 independently derived KO clones. c Diagram depicting experimental set-up, where WT and KO TSCs were used for RT-qPCR and immunofluorescence staining across a differentiation time course. d Proliferation rates of WT (n = 3 clones) and KO ( n = 3 clones) TSCs in differentiation conditions (Remove) over a 4-day period. No differences were observed. Statistical analysis was performed by two-sided unpaired t-test per day. Data are displayed as mean +/- SEM. e Immunofluorescence staining of WT and KO TSCs after 4 days of differentiation against cell membrane marker ZO1, depicting the presence of a smooth continuous ZO1-stained cell border in non-syncytial, single cells in the WT clone, and complete syncytialisation in the KO clone. Images are representative of n = 4 WT clones and n = 3 KO clones. f Bar chart showing the significant increase in syncytialized cells in KO ( n = 3 clones) compared to WT TSCs at 3 days (3D) and 4 days (4D) of differentiation (WT n = 5 TSC clones at 3D and n = 4 at 4D). Statistical significance was calculated using two-sided unpaired t-test; data are plotted as mean +/- SEM. *** * p < 0.0001. g RT-qPCR analysis of wild-type (WT, n = 5 clones) and Cxadr knockout (KO, n = 3 clones) TSC clones grown in stem cell conditions and after 2-, 3-, and 4-days of differentiation in Remove conditions. Genes assessed included TSC markers ( Cdx2 , Eomes ), and markers of the syncytiotrophoblast layers II ( = SynT-II; Gcm1 , Synb , Gabrp ) and SynT-I ( Syna , Slc16a / Mct1 , Atp11a , Hbegf ). Data are normalized to WT TSCs in stem cell conditions and plotted as mean +/- SEM. Statistical significance was calculated using 2-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Source data and full P values for d, f, and g are provided as a Source Data file.

Techniques: CRISPR, Immunofluorescence, Staining, Knock-Out, Membrane, Derivative Assay, Clone Assay, Quantitative RT-PCR, Marker

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1) Product Images from "Vitamin D Binding Protein, a Ligand of Integrin beta 1, Motivates Both Tumor Cells and Schwann Cells to Promote Perineural Invasion in Pancreatic Ductal Adenocarcinoma"

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