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custom dna array  (Azenta)

 
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    Structured Review

    Azenta custom dna array
    Determining assembly efficiency by gel electrophoresis, related to step <t>7</t> <t>sgRNA</t> library insert (∼140 bp), digested pLentiCRISPR_v2 backbone (∼13,000 bp), and HiFi assembly reaction product were resolved on a 2% TAE agarose gel. Electrophoresis was performed at 120 V for 30 min in 1× TAE buffer, followed by SYBR green staining and UV visualization. 1 Kb <t>DNA</t> ladder were included for size validation.
    Custom Dna Array, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom+array/array+custom+dna/pmc12478239-239-8-19
    Average 86 stars, based on 1 article reviews
    custom dna array - by Bioz Stars, 2026-09
    86/100 stars

    Images

    1) Product Images from "In vivo CRISPR screening protocol to identify metastasis mediators using iteratively selected mouse models"

    Article Title: In vivo CRISPR screening protocol to identify metastasis mediators using iteratively selected mouse models

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2025.104042

    Determining assembly efficiency by gel electrophoresis, related to step 7 sgRNA library insert (∼140 bp), digested pLentiCRISPR_v2 backbone (∼13,000 bp), and HiFi assembly reaction product were resolved on a 2% TAE agarose gel. Electrophoresis was performed at 120 V for 30 min in 1× TAE buffer, followed by SYBR green staining and UV visualization. 1 Kb DNA ladder were included for size validation.
    Figure Legend Snippet: Determining assembly efficiency by gel electrophoresis, related to step 7 sgRNA library insert (∼140 bp), digested pLentiCRISPR_v2 backbone (∼13,000 bp), and HiFi assembly reaction product were resolved on a 2% TAE agarose gel. Electrophoresis was performed at 120 V for 30 min in 1× TAE buffer, followed by SYBR green staining and UV visualization. 1 Kb DNA ladder were included for size validation.

    Techniques Used: Nucleic Acid Electrophoresis, Agarose Gel Electrophoresis, SYBR Green Assay, Staining, Biomarker Discovery

    Related Articles

    DNA Array:

    Article Title: In vivo CRISPR screening protocol to identify metastasis mediators using iteratively selected mouse models
    Article Snippet: Note: The trans-activating CRISPR RNA ( tracr RNA) sequence varies in the pipeline of the Genetic Perurbation Platform (GPP) tool ( https://portals.broadinstitute.org/gpp/public/ , gtttv for the Chen version and gtttt for the Hsu version)., 3. .. Synthesize the pooled sgRNA oligo library as a custom DNA array using a commercial high-throughput synthesis service (for instance, GeneWiz or GenScript). ..

    Article Title: In vivo CRISPR screening protocol to identify metastasis mediators using iteratively selected mouse models.
    Article Snippet: .. Synthesize the pooled sgRNA oligo library as a custom DNA array using a commercial high- throughput synthesis service (for instance, GeneWiz or GenScript). ..

    High Throughput Screening Assay:

    Article Title: In vivo CRISPR screening protocol to identify metastasis mediators using iteratively selected mouse models.
    Article Snippet: .. Synthesize the pooled sgRNA oligo library as a custom DNA array using a commercial high- throughput synthesis service (for instance, GeneWiz or GenScript). ..



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    custom dna array  (Azenta)
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    Azenta custom dna array
    Determining assembly efficiency by gel electrophoresis, related to step <t>7</t> <t>sgRNA</t> library insert (∼140 bp), digested pLentiCRISPR_v2 backbone (∼13,000 bp), and HiFi assembly reaction product were resolved on a 2% TAE agarose gel. Electrophoresis was performed at 120 V for 30 min in 1× TAE buffer, followed by SYBR green staining and UV visualization. 1 Kb <t>DNA</t> ladder were included for size validation.
    Custom Dna Array, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom+array/array+custom+dna/pmc12478239-239-8-19
    Average 86 stars, based on 1 article reviews
    custom dna array - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Determining assembly efficiency by gel electrophoresis, related to step 7 sgRNA library insert (∼140 bp), digested pLentiCRISPR_v2 backbone (∼13,000 bp), and HiFi assembly reaction product were resolved on a 2% TAE agarose gel. Electrophoresis was performed at 120 V for 30 min in 1× TAE buffer, followed by SYBR green staining and UV visualization. 1 Kb DNA ladder were included for size validation.

    Journal: STAR Protocols

    Article Title: In vivo CRISPR screening protocol to identify metastasis mediators using iteratively selected mouse models

    doi: 10.1016/j.xpro.2025.104042

    Figure Lengend Snippet: Determining assembly efficiency by gel electrophoresis, related to step 7 sgRNA library insert (∼140 bp), digested pLentiCRISPR_v2 backbone (∼13,000 bp), and HiFi assembly reaction product were resolved on a 2% TAE agarose gel. Electrophoresis was performed at 120 V for 30 min in 1× TAE buffer, followed by SYBR green staining and UV visualization. 1 Kb DNA ladder were included for size validation.

    Article Snippet: Synthesize the pooled sgRNA oligo library as a custom DNA array using a commercial high-throughput synthesis service (for instance, GeneWiz or GenScript).

    Techniques: Nucleic Acid Electrophoresis, Agarose Gel Electrophoresis, SYBR Green Assay, Staining, Biomarker Discovery